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The map label for this gene is pleD [H]

Identifier: 78046489

GI number: 78046489

Start: 1060995

End: 1061459

Strand: Reverse

Name: pleD [H]

Synonym: XCV0933

Alternate gene names: 78046489

Gene position: 1061459-1060995 (Counterclockwise)

Preceding gene: 78046490

Following gene: 78046486

Centisome position: 20.5

GC content: 64.73

Gene sequence:

>465_bases
ATGACACGCGACGAAATTCTCGGTTCCCGCATCCTCATCGTCGACGACGAGCCGGCCAACGTGCGGTTGCTCGAAGACTT
GCTGCAACGCGAAGGTTTCCAGCAAGTGATCGCCACCACCGATCCGCTACGGGTCATGGGCCTGGTCTCGGCATTTGCCC
CGGACCTGATCCTGCTGGATCTGAAGATGCCCGAGTTGGACGGCTACGCGCTGCTGGAACAGCTGGCGCGGCTGTCAGAC
CCTGGCCAGTTTCTGCCGGTGATCGTGCTCACCGCCGACCCCAGCCGCAGCGCGCGCCACCGCGCCCTGGGCCTGGGCGC
CAAGGACTTTTTGACCAAGCCGCTGGATACCTTCGAAGTCGCACTGCGGGTCTGGAACGTGGCCGAAACCACCGTGTTGT
TCAAGCGTCTGCGCGCATTGGCCGCGCCGGATGCAGCGCCGCCGGGGTGGCGTCAGCAGGAATGA

Upstream 100 bases:

>100_bases
CCGCCACACTGGCGCGCGTGGGCAATCAAGGCGTGCGCGCCTATCTGACCAAGCCGCTCGACGTGGCCGAATTCTTCACC
GTAATCGATGGGTTGCTGGC

Downstream 100 bases:

>100_bases
CGCGCATACATCGTAGGAGCGCACCTGGGCGCGACGTGGCGTTTATCGGCAGAGCCTCGTCGCGCCCGGGTGCGCTCCTA
CGACTGGCGCTGCGCTACAG

Product: two-component system regulatory protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTRDEILGSRILIVDDEPANVRLLEDLLQREGFQQVIATTDPLRVMGLVSAFAPDLILLDLKMPELDGYALLEQLARLSD
PGQFLPVIVLTADPSRSARHRALGLGAKDFLTKPLDTFEVALRVWNVAETTVLFKRLRALAAPDAAPPGWRQQE

Sequences:

>Translated_154_residues
MTRDEILGSRILIVDDEPANVRLLEDLLQREGFQQVIATTDPLRVMGLVSAFAPDLILLDLKMPELDGYALLEQLARLSD
PGQFLPVIVLTADPSRSARHRALGLGAKDFLTKPLDTFEVALRVWNVAETTVLFKRLRALAAPDAAPPGWRQQE
>Mature_153_residues
TRDEILGSRILIVDDEPANVRLLEDLLQREGFQQVIATTDPLRVMGLVSAFAPDLILLDLKMPELDGYALLEQLARLSDP
GQFLPVIVLTADPSRSARHRALGLGAKDFLTKPLDTFEVALRVWNVAETTVLFKRLRALAAPDAAPPGWRQQE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=140, Percent_Identity=36.4285714285714, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=117, Percent_Identity=36.7521367521368, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788192, Length=135, Percent_Identity=31.1111111111111, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1788550, Length=142, Percent_Identity=30.9859154929577, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1787487, Length=110, Percent_Identity=34.5454545454545, Blast_Score=61, Evalue=4e-11,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=36.2068965517241, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 17096; Mature: 16965

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDEILGSRILIVDDEPANVRLLEDLLQREGFQQVIATTDPLRVMGLVSAFAPDLILLD
CCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEE
LKMPELDGYALLEQLARLSDPGQFLPVIVLTADPSRSARHRALGLGAKDFLTKPLDTFEV
ECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHHHHH
ALRVWNVAETTVLFKRLRALAAPDAAPPGWRQQE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TRDEILGSRILIVDDEPANVRLLEDLLQREGFQQVIATTDPLRVMGLVSAFAPDLILLD
CCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEE
LKMPELDGYALLEQLARLSDPGQFLPVIVLTADPSRSARHRALGLGAKDFLTKPLDTFEV
ECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHHHHH
ALRVWNVAETTVLFKRLRALAAPDAAPPGWRQQE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA