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The map label for this gene is purH [H]

Identifier: 78046103

GI number: 78046103

Start: 614405

End: 615988

Strand: Reverse

Name: purH [H]

Synonym: XCV0547

Alternate gene names: 78046103

Gene position: 615988-614405 (Counterclockwise)

Preceding gene: 78046112

Following gene: 78046102

Centisome position: 11.9

GC content: 66.35

Gene sequence:

>1584_bases
ATGGCCTCTGATTTCCTGCCCGTGCGCCGGGCCCTGCTCTCCGTTTCCGACAAGACCGGCCTGATCGACCTGGCCCGCGC
GCTGGTGGCGCGCAACGTCGAGCTGCTGTCCACCGGCGGCACCGCCAAGGCGATCCGCGAGGCCGGCCTGCCGGTGAAGG
ACGTTGCCGAGCTGACCGGTTTCCCGGAAATGATGGATGGCCGCGTCAAGACCCTGCACCCGCTGGTGCACGGCGGCCTG
CTCGGGCGCGCCGGTGTCGACGAAGCGGTGATGGCCGAGCACGGCATCGTGCCGATCGACCTGCTGGTATTGAACCTGTA
TCCATTCGAGTCGGTCACGGTGAAAGCCGATTGCACGCTGGCCGATGCGGTGGAAAACATCGACATCGGCGGCCCGGCGA
TGTTGCGCAGTGCGGCCAAGAATTTCGCGCGCGTGGCCGTGGCCACCGACCCGGCGCAGTACGCCGACCTGCTGGCCGAG
CTGGAAGTCAACGACGGGCAGCTGTCGGCGGCCAAGCGCTTTGCGCTGTCGGTGGCTGCGTTCAATCGCGTGGCCCAGTA
CGACGCGGCGATCAGTAACTATCTCTCGGCAGTGGCCGACAGCAGCGAGGCGGTGCCCACGCGCAGCCCGTTCCCGGCGC
AGATCAATTCCAACTTCGTCAAGGTGATGGACCTGCGTTACGGTGAGAACCCGCACCAGTCCGGCGCGTTCTACCGCGAC
CTGTATCCGGTGCCGGGCACGCTGGCCACCTTCCAGCAGTTGCAGGGCAAGGAACTGAGCTACAACAACCTGGCCGATGC
CGATGCGGCGTGGGAATGCGTGCGCCAGTTCGACGCGCCGGCCTGCGTGATCGTCAAGCACGCCAACCCCTGCGGCGTGG
CGGTGGGCGCGGGCTGCGGCGATGCCTATGAACTGGCCTACGCCACCGACCCCACCAGCGCGTTCGGCGGCATCCTGGCG
TTCAACAAGACCCTGGACGCAGCGACGGCAAAGGCCATTCTGGACCGCCAGTTCGTCGAAGTGCTGATCGCGCCCGACTA
CGAGGCCGGCGCACTGGAATACGCCACCAGGAAGGCCAACGTGCGCGTGCTCAAGATTCCGCACGGCAATGGCTTGAACA
ACTACGACACCAAGCGGATCGGCTCGGGCCTGTTGATGCAGTCGGCCGACAACCGCAGCATGTCGCTGGGCGAGTTGAGC
GTGGTCACCCAACGTGCGCCCAACGAGGCAGAACTGGGCGATTTGCTGTTTGCCTGGCGCGTGGCCAAGTACGTCAAATC
CAACGCGATCGTCTATGCCAAGGACAGCCGCACCATTGGCGTGGGCGCCGGGCAGATGAGCCGCGTGGTCAGCGCCAAGA
TTGCCGCGCTCAAGGCCGAAGAAGCCAAGCTCACCGTGGCCGGCTCGGTGATGGCATCGGACGCGTTCTTCCCGTTCCGC
GACGGCATCGATGCGGCGGCTGCGGCCGGCATCAAGGCGGTGATCCAGCCGGGCGGTTCGATGCGCGATGGCGAGGTGAT
CGCCGCCGCCGACGAACACGGCATCGCGATGGTGTTCACCGGCGTGCGCCACTTCCGGCATTGA

Upstream 100 bases:

>100_bases
TCCCTTCAGTGCATAGATCGAGGCCATACGGGGTGTTGAGTCCATTCGAGCGCTGGCGCAGCCTACAATATCGGCTGCTC
CGCAACGACATCCCCCACCG

Downstream 100 bases:

>100_bases
TGTCACCGGCACAGGGATAGGTCGATGCAGCGTCTGCGTTGTTTTGCCGCGTTTCTTGCCACCGCCGCGCTGTTGAGCGC
GTGCAAGCAGGCGCCTGCGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGL
LGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTLADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAE
LEVNDGQLSAAKRFALSVAAFNRVAQYDAAISNYLSAVADSSEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCGDAYELAYATDPTSAFGGILA
FNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATRKANVRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRSMSLGELS
VVTQRAPNEAELGDLLFAWRVAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH

Sequences:

>Translated_527_residues
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGL
LGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTLADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAE
LEVNDGQLSAAKRFALSVAAFNRVAQYDAAISNYLSAVADSSEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCGDAYELAYATDPTSAFGGILA
FNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATRKANVRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRSMSLGELS
VVTQRAPNEAELGDLLFAWRVAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH
>Mature_526_residues
ASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGLL
GRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTLADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAEL
EVNDGQLSAAKRFALSVAAFNRVAQYDAAISNYLSAVADSSEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRDL
YPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCGDAYELAYATDPTSAFGGILAF
NKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATRKANVRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRSMSLGELSV
VTQRAPNEAELGDLLFAWRVAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFRD
GIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=511, Percent_Identity=36.7906066536204, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=60.3053435114504, Blast_Score=627, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=611, Percent_Identity=33.3878887070376, Blast_Score=274, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI71985574, Length=350, Percent_Identity=28, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=36.1344537815126, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=481, Percent_Identity=36.5904365904366, Blast_Score=269, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6323056, Length=484, Percent_Identity=36.3636363636364, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24649832, Length=507, Percent_Identity=39.0532544378698, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55788; Mature: 55657

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPLVHGGLLGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCH
ADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAELEVNDGQLSAAKRFALSVAA
HHHHHHCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FNRVAQYDAAISNYLSAVADSSEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEHC
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCG
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCC
DAYELAYATDPTSAFGGILAFNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATRKAN
CCEEEEEECCCCHHHCCCEEECCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCC
VRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRSMSLGELSVVTQRAPNEAELGDLLFAWR
EEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHH
VAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCC
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH
CCCCHHHHHCHHEEECCCCCCCCCCEEEEECCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
ASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIREAGLPVKDVAELTG
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPLVHGGLLGRAGVDEAVMAEHGIVPIDLLVLNLYPFESVTVKADCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCH
ADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAELEVNDGQLSAAKRFALSVAA
HHHHHHCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FNRVAQYDAAISNYLSAVADSSEAVPTRSPFPAQINSNFVKVMDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEHC
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGAGCG
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCC
DAYELAYATDPTSAFGGILAFNKTLDAATAKAILDRQFVEVLIAPDYEAGALEYATRKAN
CCEEEEEECCCCHHHCCCEEECCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCC
VRVLKIPHGNGLNNYDTKRIGSGLLMQSADNRSMSLGELSVVTQRAPNEAELGDLLFAWR
EEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHH
VAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCC
DGIDAAAAAGIKAVIQPGGSMRDGEVIAAADEHGIAMVFTGVRHFRH
CCCCHHHHHCHHEEECCCCCCCCCCEEEEECCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]