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The map label for this gene is purD [H]

Identifier: 78046101

GI number: 78046101

Start: 612207

End: 613502

Strand: Reverse

Name: purD [H]

Synonym: XCV0545

Alternate gene names: 78046101

Gene position: 613502-612207 (Counterclockwise)

Preceding gene: 78046102

Following gene: 78046100

Centisome position: 11.85

GC content: 68.83

Gene sequence:

>1296_bases
TTGAAAATCCTCGTCATCGGCTCCGGCGGCCGTGAACATGCCCTGGCCTGGAAGATCGCGCAGTCCGCGCGCGTGCGCGA
GGTGCTGGTGGCACCGGGCAATGCCGGCACCGCCACCGAAGCCAAATGCCGCAATGTCGCGATCAAGGTCAATGATCTGG
ACGGCCTGCTCGCGCTGGCGCAGCGCGAAGCCGTCGCGCTGACCGTGGTCGGCCCGGAAGTGCCGCTGGTGCTGGGCGTG
GTCGACCGCTTCCATGCCGCCGGCCTGCGCATCTTCGGCCCCACCGCCAAGGCCGCGCAGCTGGAAGGCAGCAAGGCCTT
CGCCAAGGATTTCCTCGCCCGCCACGGCATTCCCACCGCGTATTACGCCGTGCACACCGAAGTCGACGCCGCGCTGGCGT
ATGTGCGCGAGAAAGGCGCGCCGATCGTGGTCAAGGCCGATGGCCTGGCTGCCGGCAAGGGCGTGATCGTGGCGATGACC
CTGGCCGAGGCCGAAGACGCGGTGCGCGACATGCTCTCGGGCAATGCGTTCGGCGATGCCGGCGCACGCGTGGTCATCGA
AGAATTCCTCGACGGCGAAGAGGCCAGCTTCATCTCCATGGTCGATGGCAAACACGCCTTGCCGATGGCCACCAGCCAGG
ACCACAAGCGCGTCGGCGACGGCGACACCGGCCCCAATACCGGCGGCATGGGCGCCTACTCGCCGGCGCCGGTGGTCACG
CCCGAGGTGCATGCGCGGGTCATGCGCGAGGTGGTCGAGCCGACCGTGCAGGGCATGATCGCCGATGGCGTGCCGTTCAC
CGGTTTTCTGTATGCCGGGCTGATGATCGACGCGCACGGCGCGCCGAAGGTGATCGAATTCAACGTGCGTTTCGGCGACC
CGGAAACCCAGCCGGTAATGCTGCGCCTGCAGTCGGATCTGGTCGATCTGCTCGACGCGGCCATCGACGGCAAGCTCGAT
GGCGCGCACGCGCAGTGGGACCCGCGCCCGTCGCTGGGCGTGGTCATCGCCGCCAAGCCTTACCCGGACACACCGGTCAC
CGGTGAGGTCATCACCGGGCTTGACGCGGTGCCGGCCAGCGCCAAGGTTTTCCACGCCGGCACTGCGCTGAATGCCGCCG
GCGAGGTGATCAGCGCCGGTGGCCGCGTGCTGTGCGTTGCGGCGCTCGGCGACAGCGTGCAGGACGCCCAGCGCAACGCC
TATGCTGGCCTCAAGCCGATCCACTGGCCCAACGCCTTCCAGCGCAGCGACATCGGCTGGCGGGCCATCGCCCGCGAGCA
GGCGGCGCAGGGCTAA

Upstream 100 bases:

>100_bases
TCGCGCTCGACACCAAGACCTGGACCTACCCGTTTCCCAATGCACTGCAGCGCGAAGGCTGCAGGTAACGCCCCTTCCCC
CGTACTAGCGAGCAACTGCC

Downstream 100 bases:

>100_bases
GCGCCTCGCGCCTGCAACCACCGCGTTGCGCGCTGTCGCCACGCGGTGGTTGCAGGTTCCTGCGCACCACCAGGCATCCT
GCCGATACCGGCGCGGGCCA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKILVIGSGGREHALAWKIAQSARVREVLVAPGNAGTATEAKCRNVAIKVNDLDGLLALAQREAVALTVVGPEVPLVLGV
VDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTAYYAVHTEVDAALAYVREKGAPIVVKADGLAAGKGVIVAMT
LAEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVMLRLQSDLVDLLDAAIDGKLD
GAHAQWDPRPSLGVVIAAKPYPDTPVTGEVITGLDAVPASAKVFHAGTALNAAGEVISAGGRVLCVAALGDSVQDAQRNA
YAGLKPIHWPNAFQRSDIGWRAIAREQAAQG

Sequences:

>Translated_431_residues
MKILVIGSGGREHALAWKIAQSARVREVLVAPGNAGTATEAKCRNVAIKVNDLDGLLALAQREAVALTVVGPEVPLVLGV
VDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTAYYAVHTEVDAALAYVREKGAPIVVKADGLAAGKGVIVAMT
LAEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVMLRLQSDLVDLLDAAIDGKLD
GAHAQWDPRPSLGVVIAAKPYPDTPVTGEVITGLDAVPASAKVFHAGTALNAAGEVISAGGRVLCVAALGDSVQDAQRNA
YAGLKPIHWPNAFQRSDIGWRAIAREQAAQG
>Mature_431_residues
MKILVIGSGGREHALAWKIAQSARVREVLVAPGNAGTATEAKCRNVAIKVNDLDGLLALAQREAVALTVVGPEVPLVLGV
VDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTAYYAVHTEVDAALAYVREKGAPIVVKADGLAAGKGVIVAMT
LAEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVMLRLQSDLVDLLDAAIDGKLD
GAHAQWDPRPSLGVVIAAKPYPDTPVTGEVITGLDAVPASAKVFHAGTALNAAGEVISAGGRVLCVAALGDSVQDAQRNA
YAGLKPIHWPNAFQRSDIGWRAIAREQAAQG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=48.951048951049, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=48.951048951049, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=48.951048951049, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=48.951048951049, Blast_Score=394, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=70.0934579439252, Blast_Score=588, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=40.9411764705882, Blast_Score=301, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=41.4798206278027, Blast_Score=347, Evalue=2e-96,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.3488372093023, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.3488372093023, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44914; Mature: 44914

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50975 ATP_GRASP ; PS00583 PFKB_KINASES_1 ; PS00178 AA_TRNA_LIGASE_I ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGSGGREHALAWKIAQSARVREVLVAPGNAGTATEAKCRNVAIKVNDLDGLLALA
CEEEEEECCCCCCHHEEHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHH
QREAVALTVVGPEVPLVLGVVDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTA
CCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCHHHCCCCHHHHHHHHHHCCCCEE
YYAVHTEVDAALAYVREKGAPIVVKADGLAAGKGVIVAMTLAEAEDAVRDMLSGNAFGDA
EEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCC
GARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEECCCCCEEEEEEEEECCCCCCCEE
LRLQSDLVDLLDAAIDGKLDGAHAQWDPRPSLGVVIAAKPYPDTPVTGEVITGLDAVPAS
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCC
AKVFHAGTALNAAGEVISAGGRVLCVAALGDSVQDAQRNAYAGLKPIHWPNAFQRSDIGW
CEEEECCCHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCH
RAIAREQAAQG
HHHHHHHHCCC
>Mature Secondary Structure
MKILVIGSGGREHALAWKIAQSARVREVLVAPGNAGTATEAKCRNVAIKVNDLDGLLALA
CEEEEEECCCCCCHHEEHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHH
QREAVALTVVGPEVPLVLGVVDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTA
CCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCHHHCCCCHHHHHHHHHHCCCCEE
YYAVHTEVDAALAYVREKGAPIVVKADGLAAGKGVIVAMTLAEAEDAVRDMLSGNAFGDA
EEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCC
GARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEECCCCCEEEEEEEEECCCCCCCEE
LRLQSDLVDLLDAAIDGKLDGAHAQWDPRPSLGVVIAAKPYPDTPVTGEVITGLDAVPAS
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCC
AKVFHAGTALNAAGEVISAGGRVLCVAALGDSVQDAQRNAYAGLKPIHWPNAFQRSDIGW
CEEEECCCHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCH
RAIAREQAAQG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]