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The map label for this gene is phaD [H]

Identifier: 78046046

GI number: 78046046

Start: 553077

End: 554621

Strand: Reverse

Name: phaD [H]

Synonym: XCV0490

Alternate gene names: 78046046

Gene position: 554621-553077 (Counterclockwise)

Preceding gene: 78046047

Following gene: 78046045

Centisome position: 10.71

GC content: 66.41

Gene sequence:

>1545_bases
ATGAACCATCTGCTGATCCTGCCCATCCTGATCCCGATGCTGGGCGCGTCGGCATCGTTGTTCGTCGAGCACCGCCGCTA
TGGGCCGCGCGTGCAGCGCAGCGTGGCGTGGGTCAGCATCGCCTTGTTGGCCGCGGCGGTCATCGCCCTGTTCGCACGCG
CGGCCGACGGCCGGATCCTGGTCTATCTGCTCGGCGACTGGCCGGCGCGCATCGGCATCGTGCTGATGGGCGACCGCTTG
TCGGCGTGGATGCTGCTGACGACCTTGATCCTGGGCTCGGCGTGCCTGCTGCATGCATGCGCAGGCTGGGACCGGCGCGC
ACCGCATTTCCATGCATTGTTTCAGTTCCAGTTGATGGGCTTGAACGGCGCATTCCTGACCGGCGACATCTTCAACCTGT
TCGTGTTCTTCGAGGTGATGCTCATCGCCTCCTACGGCCTGCTGCTCAGCGGCGGGCGCGGGTTGCAGATGCGGGTGGGC
CTGCATTACGTGGTGTTCAACGTGTGTGCGTCGACGTTGTTCCTGATTGCGCTGGGCCTGCTGTTCGGTGTTTTCGGCAC
GCTCAACATGGCCGAGCTGTCGCAGCGCATTGCCGATCTGCCGGCGCAGGACCTGCCGTTGGCAAAGGCCGCGCTGGGCT
TGTTGCTGCTGGTGTTCTGCAGCAAGGCCGCATTGCTGCCGCTGTATCTGTGGCTACCGGAAACCTATTCGCGTGCGCCG
GCGGCGGTGGCTGCATTGTTCGCCATCATGACCAAGGTGGGGTTATACGCAGCGCTGCGGGTATCGTCGTTGTGGTTCGG
TGCCGGTGCCGGCGCGCTGCACGGCTTCGGTCGCCACGCGCTGTTGTGGCTGGGCATCGCGACGCTGCTGATGGCCGCGT
TCGGGGTGATGGCCGCATCGCGGCTGCGGGTGATGGTGTCGTACCTGGTGGTGTTTTCGGCAGCGACCTTGTTCATCGCG
TTTTCGCTGGACGATGCCGGCGCACTGGGCGCGGGACTGTATTACCTGCCGCACAGCAGCTTTGTCGCCGCTGCGTTGTT
CATGGTCTCTGACCTGATCCGCCGTCGCCGCGGCAGTGCCAGCGACCGCAAGGAAGTGGTGGCGCCGCTGCCGGGGCGGG
CCATTCCGGGCACGATGTTCATGATTGCCGCGGTGGCGGTGGCCGGGTTGCCGCCACTCTCCGGATTTCTGGCCAAGGCC
GCATTGCTGCAACACGTGCCTGAAGGGCTGGTGGGACCGGTGTGGATCGCGGTGCTCGGCAGTAGCCTGCTGATGGTGAT
CGGCCTCACCCGCGCCGGCGTACGGTTGTTCTGGCGGGTGCCGGCGGCGGCGGAAAATGCGCCGGAACTGCAGCCGCCGC
GACGTGGCCGCATGCGTCCGGTGGAGACCGCCGCCACGGTGCTGTTGCTGAGCGGGCTGGTTGCGATGACCGGCGCCGCA
GGTCCCCTGATGCATTACACCGATGCGGCCGCTGCGCAGTTGCGCGACCCCGGCGCGTATGTTGACCAGGTCCGCGCCAC
CACGCCGCAACGGAGGCAGCCATGA

Upstream 100 bases:

>100_bases
GCCTGGTGCTGGCGATCCGCAGCCGTAGCGACAATGGCAGCGACCATGTGGATGCGCACGAAGATGCCGCGACCGGCACG
GATACGCGGCAGGCCCGGCG

Downstream 100 bases:

>100_bases
ACACACGTCTGCCACTGCGTCGCCGCCTGTTTCCCTCGCCGCCCTTGAGCGTGACGGTCTTCGTGTTCTGGCTGCTGCTC
AGCGATAGCTTCGGCCTGCA

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSIALLAAAVIALFARAADGRILVYLLGDWPARIGIVLMGDRL
SAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVG
LHYVVFNVCASTLFLIALGLLFGVFGTLNMAELSQRIADLPAQDLPLAKAALGLLLLVFCSKAALLPLYLWLPETYSRAP
AAVAALFAIMTKVGLYAALRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSASDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLSGFLAKA
ALLQHVPEGLVGPVWIAVLGSSLLMVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLSGLVAMTGAA
GPLMHYTDAAAAQLRDPGAYVDQVRATTPQRRQP

Sequences:

>Translated_514_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSIALLAAAVIALFARAADGRILVYLLGDWPARIGIVLMGDRL
SAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVG
LHYVVFNVCASTLFLIALGLLFGVFGTLNMAELSQRIADLPAQDLPLAKAALGLLLLVFCSKAALLPLYLWLPETYSRAP
AAVAALFAIMTKVGLYAALRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSASDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLSGFLAKA
ALLQHVPEGLVGPVWIAVLGSSLLMVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLSGLVAMTGAA
GPLMHYTDAAAAQLRDPGAYVDQVRATTPQRRQP
>Mature_514_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSIALLAAAVIALFARAADGRILVYLLGDWPARIGIVLMGDRL
SAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVG
LHYVVFNVCASTLFLIALGLLFGVFGTLNMAELSQRIADLPAQDLPLAKAALGLLLLVFCSKAALLPLYLWLPETYSRAP
AAVAALFAIMTKVGLYAALRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSASDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLSGFLAKA
ALLQHVPEGLVGPVWIAVLGSSLLMVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLSGLVAMTGAA
GPLMHYTDAAAAQLRDPGAYVDQVRATTPQRRQP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788829, Length=498, Percent_Identity=24.4979919678715, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788613, Length=428, Percent_Identity=24.2990654205607, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54871; Mature: 54871

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSIALLAAAVIALFARAADGRIL
CCCEEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VYLLGDWPARIGIVLMGDRLSAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMG
EEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEC
LNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVGLHYVVFNVCASTLFLIALGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LFGVFGTLNMAELSQRIADLPAQDLPLAKAALGLLLLVFCSKAALLPLYLWLPETYSRAP
HHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AAVAALFAIMTKVGLYAALRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLRVMVSYLVVFSAATLFIAFSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSA
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCC
SDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLSGFLAKAALLQHVPEGLVGPVWIAVLG
CCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SSLLMVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLSGLVAMTGAA
HHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GPLMHYTDAAAAQLRDPGAYVDQVRATTPQRRQP
CCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRSVAWVSIALLAAAVIALFARAADGRIL
CCCEEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VYLLGDWPARIGIVLMGDRLSAWMLLTTLILGSACLLHACAGWDRRAPHFHALFQFQLMG
EEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEC
LNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRVGLHYVVFNVCASTLFLIALGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LFGVFGTLNMAELSQRIADLPAQDLPLAKAALGLLLLVFCSKAALLPLYLWLPETYSRAP
HHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AAVAALFAIMTKVGLYAALRVSSLWFGAGAGALHGFGRHALLWLGIATLLMAAFGVMAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLRVMVSYLVVFSAATLFIAFSLDDAGALGAGLYYLPHSSFVAAALFMVSDLIRRRRGSA
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCC
SDRKEVVAPLPGRAIPGTMFMIAAVAVAGLPPLSGFLAKAALLQHVPEGLVGPVWIAVLG
CCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SSLLMVIGLTRAGVRLFWRVPAAAENAPELQPPRRGRMRPVETAATVLLLSGLVAMTGAA
HHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GPLMHYTDAAAAQLRDPGAYVDQVRATTPQRRQP
CCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]