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The map label for this gene is hrcU [H]

Identifier: 78045982

GI number: 78045982

Start: 476245

End: 477318

Strand: Reverse

Name: hrcU [H]

Synonym: XCV0426

Alternate gene names: 78045982

Gene position: 477318-476245 (Counterclockwise)

Preceding gene: 78045992

Following gene: 78045981

Centisome position: 9.22

GC content: 61.17

Gene sequence:

>1074_bases
ATGTCGGAAGAAAAAACCGAGAAGCCGACCGAGAAGAAACTGCGGGATGCGCGCAAGGACGGGGAAGTTCCGGTCAGCCC
GGACGTCACTGCAGCGGCGGTGCTCTTCGGCGCGTTATTGGTGATGAAATCGGCCGGCGATTACTTCTCCGATCACATGC
GCGCATTGATGACGATCGGCTTCGATTTTTCCGAACATACACGCGATGCAGCGGCCATCAACCGCGCACTCGGCCATATC
GGCATCCAGGGATTGCTGTTGATGCTGCCGTTCCTGGCTGCCTGCCTGGTGGCCGGTATGGCGGGCGGTGCGTTCCAGAC
TGGCCTGAATGCCTCGCTCAAGCCGGTGGCGCCCAAGTTCGATTCCCTCAATCCCGCAACCGGGGTCAAGAAACTCTTTT
CGTTGCGAAGCCTCATCAACTTGCTGAAGTTGATCATCAAGGCCATCTTGATTGGCGTGGTGCTGTGGGTCGGTATCCGC
GCCCTCATGCCGATGATCATTGGGCTTGCCTACGAGACACCGCTTGACGTTGCGCAAATTGCCTGGCGCACCCTGGGCAT
GCTCTTCGCACTGGGCGTGCTGCTGTTCGTCCTGGTCGGCGCTGCAGACTGGAGCGTGCAGCGCTGGCTGTTCATCCGCG
ACAAGCGAATGAGCAAGGACGAGCAGAAGCGCGAAGTCAAGGAAAGCGAGGGCGACCCCGAAATCAAGGACAAGCGCAAG
GAATTCGCCAAGGAGCTGGTCTTCGGCGACCCGCGCGAGCGCGTGGCCAAGGCCAAGGTGATGGTGGTCAACCCGACCCA
TTACGCAGTGGCGCTGGCCTACGAGCCCGACGACTTCGGCTTGCCGCAGGTCGTTGCCAAGGGCGTGGACGACGGCGCAC
TGGAACTGCGCGCCTTCGCGCATAACCAGGGCATTCCGATCGTTGCCAATCCGCCACTGGCGCGTGCGCTGTATCAGGTT
GAATTGGGCGATGCAATCCCCGAGCCACTGTTCGAAACGGTTGCGGTGGTGTTGCGCTGGGTCGATGAACTCGGTCGCGA
CCACGGCGACGGCGATGGAGCCCTGCCATGCTAG

Upstream 100 bases:

>100_bases
TCCAGCTTCGCCAGGCCATCCACACATTCGCCTGGAAAACGGATCGCTCCACCACAGCTGCCCACAATCGGGGGCGCAGC
GATCGCGGAAGGAGAAGGCA

Downstream 100 bases:

>100_bases
GAGACCGCGTGCGAGCGACAAGGTATTTCGCTTATAGCGGCGAAGTAGCCATCGCCGCCCTGGTAGTGGCGGTCATCGGG
CTGATGATCCTGCCGCTGCC

Product: type III secretion system protein HrcU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFSDHMRALMTIGFDFSEHTRDAAAINRALGHI
GIQGLLLMLPFLAACLVAGMAGGAFQTGLNASLKPVAPKFDSLNPATGVKKLFSLRSLINLLKLIIKAILIGVVLWVGIR
ALMPMIIGLAYETPLDVAQIAWRTLGMLFALGVLLFVLVGAADWSVQRWLFIRDKRMSKDEQKREVKESEGDPEIKDKRK
EFAKELVFGDPRERVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFAHNQGIPIVANPPLARALYQV
ELGDAIPEPLFETVAVVLRWVDELGRDHGDGDGALPC

Sequences:

>Translated_357_residues
MSEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFSDHMRALMTIGFDFSEHTRDAAAINRALGHI
GIQGLLLMLPFLAACLVAGMAGGAFQTGLNASLKPVAPKFDSLNPATGVKKLFSLRSLINLLKLIIKAILIGVVLWVGIR
ALMPMIIGLAYETPLDVAQIAWRTLGMLFALGVLLFVLVGAADWSVQRWLFIRDKRMSKDEQKREVKESEGDPEIKDKRK
EFAKELVFGDPRERVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFAHNQGIPIVANPPLARALYQV
ELGDAIPEPLFETVAVVLRWVDELGRDHGDGDGALPC
>Mature_356_residues
SEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFSDHMRALMTIGFDFSEHTRDAAAINRALGHIG
IQGLLLMLPFLAACLVAGMAGGAFQTGLNASLKPVAPKFDSLNPATGVKKLFSLRSLINLLKLIIKAILIGVVLWVGIRA
LMPMIIGLAYETPLDVAQIAWRTLGMLFALGVLLFVLVGAADWSVQRWLFIRDKRMSKDEQKREVKESEGDPEIKDKRKE
FAKELVFGDPRERVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFAHNQGIPIVANPPLARALYQVE
LGDAIPEPLFETVAVVLRWVDELGRDHGDGDGALPC

Specific function: Involved in the secretion of popA, a proteinaceous elicitor of the hypersensitivity response in plants [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=347, Percent_Identity=32.5648414985591, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 38917; Mature: 38786

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFSDHMRALMTIG
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
FDFSEHTRDAAAINRALGHIGIQGLLLMLPFLAACLVAGMAGGAFQTGLNASLKPVAPKF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCC
DSLNPATGVKKLFSLRSLINLLKLIIKAILIGVVLWVGIRALMPMIIGLAYETPLDVAQI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AWRTLGMLFALGVLLFVLVGAADWSVQRWLFIRDKRMSKDEQKREVKESEGDPEIKDKRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHH
EFAKELVFGDPRERVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFA
HHHHHHHCCCHHHHHHHCEEEEECCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHH
HNQGIPIVANPPLARALYQVELGDAIPEPLFETVAVVLRWVDELGRDHGDGDGALPC
CCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFSDHMRALMTIG
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
FDFSEHTRDAAAINRALGHIGIQGLLLMLPFLAACLVAGMAGGAFQTGLNASLKPVAPKF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCC
DSLNPATGVKKLFSLRSLINLLKLIIKAILIGVVLWVGIRALMPMIIGLAYETPLDVAQI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AWRTLGMLFALGVLLFVLVGAADWSVQRWLFIRDKRMSKDEQKREVKESEGDPEIKDKRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHH
EFAKELVFGDPRERVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFA
HHHHHHHCCCHHHHHHHCEEEEECCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHH
HNQGIPIVANPPLARALYQVELGDAIPEPLFETVAVVLRWVDELGRDHGDGDGALPC
CCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8316211; 11823852 [H]