Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 78045592

GI number: 78045592

Start: 38809

End: 40290

Strand: Reverse

Name: gltD [H]

Synonym: XCV0036

Alternate gene names: 78045592

Gene position: 40290-38809 (Counterclockwise)

Preceding gene: 78045593

Following gene: 78045591

Centisome position: 0.78

GC content: 68.29

Gene sequence:

>1482_bases
ATGAGCCGCAAGCAAGCCTTCCAATTCCTCGACCTGCCCCGGACCATGCCCACGCGCATTCCGGTGGAACTGCGCACCTC
CGGCGACTGGGGCGAGTTGTACGGCAAGTTCGACAAGGCCGATGCGCAATACCAGGCCGGCCGTTGCCTGGACTGCGGCA
ATCCGTATTGCAGCTGGAAATGCCCGGTACACAACGCCATCCCGCAGTGGCTGCAGCTGGTGCAGGAAAACCGCATCGAG
GAAGCCGCCGCGCTATGCCATTCCACCAACCCGCTGCCGGAAGTGTGCGGGCGGGTGTGTCCGCAGGACCGCCTGTGCGA
AGGCAGCTGCACGCTGGAGGAATTCGGCGCAGTCACCATCGGCGCGGTGGAGAAGTACATCGTCGACACCGCCTTCAAGA
TGGGCTGGCGCCCGGACCTGGGCAACGTGCAGCCGACCGGCCACCGCGTGGCGGTGATCGGCGCCGGCCCGGCTGGGCTG
GCGTGTGCGGACCGGCTGGCGCGTGCCGGCATCGAGGCGGTGGTCTACGACCGCTATGAGCAGATCGGCGGGCTGCTGCA
GTTCGGCATCCCCAGCTTCAAGCTGGACAAGTCGGTGATCGGCAAGCGCCGCGAGATCCTCGAAGGCATGGGCGTGCAGT
TCCGCCTGGGCGTGGAGATCGGTAAGGACGTCAGCATCGAGCAGTTGCTGGGCGAATACGATGCGGTGTTCTTAGGGACC
GGCGCCTATCGCTACACCGACGGCGGCCTGCCCGGGCAAGACCTGAAGAACGTGCTGCCGGCGCTGCCGTTCCTGGTGCA
GAACAGCCGCATCGTCAGCGGCAACGACCCGCACGGCCGGCCGATCGCCGGCTGGGAAGACCAGCTCGCCCTGCCCGACC
TCAGCGGCAAGCGCGTGGTGGTGCTGGGCGGCGGCGATACCGGCATGGACTGCGTGCGCAGCGCCATTCGCCTGGGCGCG
GCCAAGGTCACCTGCGCCTACCGCCGCGACGAAGCCAGCATGCCCGGCTCGGCGCGCGAGGTGGCCAACGCGCGCGAAGA
AGGCGTGCGCTTTTTGTTCAACCGCCAGCCGCTGTCGATCGAATCCGGCGCCGACGACGAAGCCATCGGCGTGACCGTGG
TGGAAACCAGATTAGGTGAGCCCGATGCCAGCGGCCGCCGCAATGCCGTGCCGGTGGAAGGCAGCGAATCGCTGCTGGAA
GCGGACGTGGTGATCATCGCCTTCGGCTTCTCGCCCACCCTGCCCGATTGGTTGGCCTCGCAAGGCGTGGAAGCCGGCAG
CAACGGCCGCATTGTCGCCCCGGCCGACTGCAACGGCCGCCTGCCCTACCAGACCAGCAACCCCCGCCTGTTCGCCGGCG
GCGACTGCGTGCGCGGTGCGGACCTGGTGGTCACCGCAGTGGCCGAAGGCCGCGATGCGGCGGGAAGCATCGTGCAGCTG
CTGGGGGTGAACGCGCAGGTCAAGGAGCCGGCTGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGGAATTGTAAAACCGGTTCCCCGACCCTCTTCCCGTTCGTCGCGACCCGCTTTTCCGATTCTCCATTCCCCATTCCCC
ATTCTCGATTCCCAGCGTCT

Downstream 100 bases:

>100_bases
GGGCGTGAGGGCGTGAGGGCGTGAGGGCGTGAGCGTATGCTACGCGCCATGGCCAATTCCACCACAAATCTAGACAAGGC
ATCAAAGCGCGTCGCCTGGT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIE
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVQPTGHRVAVIGAGPAGL
ACADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLSGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETRLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPTLPDWLASQGVEAGSNGRIVAPADCNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
LGVNAQVKEPAAA

Sequences:

>Translated_493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIE
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVQPTGHRVAVIGAGPAGL
ACADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLSGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETRLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPTLPDWLASQGVEAGSNGRIVAPADCNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
LGVNAQVKEPAAA
>Mature_492_residues
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIEE
AAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVQPTGHRVAVIGAGPAGLA
CADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGTG
AYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLSGKRVVVLGGGDTGMDCVRSAIRLGAA
KVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETRLGEPDASGRRNAVPVEGSESLLEA
DVVIIAFGFSPTLPDWLASQGVEAGSNGRIVAPADCNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQLL
GVNAQVKEPAAA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=29.0748898678414, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=57.8838174273859, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1788811, Length=476, Percent_Identity=55.0420168067227, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI87082180, Length=458, Percent_Identity=53.056768558952, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1788468, Length=451, Percent_Identity=29.490022172949, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789243, Length=435, Percent_Identity=32.6436781609195, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17570289, Length=482, Percent_Identity=34.6473029045643, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71984108, Length=459, Percent_Identity=30.5010893246187, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17543860, Length=172, Percent_Identity=29.0697674418605, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=473, Percent_Identity=35.0951374207188, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI28574881, Length=477, Percent_Identity=37.3165618448637, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24665539, Length=477, Percent_Identity=37.3165618448637, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24665547, Length=478, Percent_Identity=37.6569037656904, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24665543, Length=478, Percent_Identity=37.6569037656904, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24640763, Length=458, Percent_Identity=31.0043668122271, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI18858217, Length=458, Percent_Identity=31.0043668122271, Blast_Score=168, Evalue=9e-42,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52725; Mature: 52594

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQAGRCLDCGNPYCSWK
CCHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCHHCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQENRIEEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFKMGWRPDLGNVQPTGHRVAVIGAGPAGLACADRLARAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
HHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLSGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETRLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPTLPDWLAS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVEAGSNGRIVAPADCNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
CCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
LGVNAQVKEPAAA
HCCCCCCCCCCCC
>Mature Secondary Structure 
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQAGRCLDCGNPYCSWK
CHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCHHCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQENRIEEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFKMGWRPDLGNVQPTGHRVAVIGAGPAGLACADRLARAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
HHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLSGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETRLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPTLPDWLAS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVEAGSNGRIVAPADCNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
CCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
LGVNAQVKEPAAA
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]