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The map label for this gene is bioF [H]

Identifier: 162138544

GI number: 162138544

Start: 454651

End: 455856

Strand: Reverse

Name: bioF [H]

Synonym: XCV0402

Alternate gene names: 162138544

Gene position: 455856-454651 (Counterclockwise)

Preceding gene: 78045959

Following gene: 78045957

Centisome position: 8.8

GC content: 67.58

Gene sequence:

>1206_bases
ATGGCTCGCCCCGATCTGCACGAACGGATTTCGTCGCTGCGTAAATTGCGTGTGGCCCAGGAACGGGTGCGGGTGCGGCG
ACAGGTGGGGCGGCGCGATGGCGTGCGGCTGGAGATCGATGGCCGTTGGCTGACCGGTTTCTGTTCCAACGACTACCTGG
GTCTGTCGCAACAGTTCGAAGTGGTCGCCGCGCTGCAGGATGCCGCTGCCCGCGATGGTGCCGGCGCCACTGCCTCGCAT
CTGATCTGCGGCCACCACACCGCGCACGAAACGCTCGAACGCGAAATCGCCGACTGGTTGGGCTATCCGTCGGCGTTGCT
GTTCGGCAGCGGCTTCATCGCCAATCTTGCCGTGCAGCAAGCGTTACTCAGCGAAGAAGACGACGTCTGCGTGCAGGACC
GGCTCAATCACGCCAGCCTGCTCGATGCCACGCGGCTGGCCGGTTGCCGGCTGCGGCGCTATCCGCACCTGGACGTGGAA
GGCGCGATGCGCCAGCTCAAGGGCGCGCCCGAAGGCGCGGCGATGCTGGCCACCGACGGCGTTTTCAGCATGGATGGCGA
TGTCGCGCCGCTGCGCGCCTTGAGCCTGGTGGCACGCATGCAGCACGCGCTGTTCTATGTAGACGACGCGCACGGTGTCG
GCGTGCTCGGTCCGCAGGGGCGCGGTTGCGTGGCCGATGCCGGGTTGGGCGTGGCCGAAGTGCCGTTGCAGTTGGTCACG
CTGGGCAAGGCGCTCGGTGGTTACGGCGCGGTGGTGGTGGGCGAAGAGGCGTTGGTGCGCCATCTGGCCGAAACTGCGCG
CCCCTACATCTACACCACTGCACTGCCGCCGGCGCAAGTCGCGGCCACCCTGGCGGCCGTGCGCCTGGCGCGTCGCGACG
ATTGGCGGCGTACGCGCCTGGTCGAATTGATCGGTGCCTTCCGCGATGGCGCGCGCAAGCACGGCTTCGAACTGATGGCC
TCCGACACGCCGATCCAGCCGCTGCTATGCGGCGAGGAAGCAACCGTGATGGCGATGTCGGCTGCGCTCGAACACGCAGG
TTTCATGGTTGGCGCCATTCGTCCGCCCACCGTGCCCGAAGGCAAGGCGCGCTTGCGCGTCACCTTGTCTGCGCTGCATA
CGCCGCAGCAGGTGCAGGCATTGATCGAGGCCATCGTGCAGGCGCGCGACGTGGTCAGTCGGCAGCCGCTGCGTGCCTCT
GCCTGA

Upstream 100 bases:

>100_bases
GCCGCCTGACCCGGCGCCGGGCACCGGTCGTCGGTGGCGGCTGGGCGCCCCGAGCCCGGCACGCTACGCTAGCGGGCCTT
CCCTGCCGTTGAACGCCCCC

Downstream 100 bases:

>100_bases
GACACGCGGCAGTGCGTGGCTGGCCTGCATGCGAGCACGCGTGGGCCACTCAGCCAATGCAGCGATTGCACGACGCAGCT
GCATGGCAGGCGACAACGGC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVVAALQDAAARDGAGATASH
LICGHHTAHETLEREIADWLGYPSALLFGSGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVE
GAMRQLKGAPEGAAMLATDGVFSMDGDVAPLRALSLVARMQHALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRTRLVELIGAFRDGARKHGFELMA
SDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPEGKARLRVTLSALHTPQQVQALIEAIVQARDVVSRQPLRAS
A

Sequences:

>Translated_401_residues
MARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVVAALQDAAARDGAGATASH
LICGHHTAHETLEREIADWLGYPSALLFGSGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVE
GAMRQLKGAPEGAAMLATDGVFSMDGDVAPLRALSLVARMQHALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRTRLVELIGAFRDGARKHGFELMA
SDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPEGKARLRVTLSALHTPQQVQALIEAIVQARDVVSRQPLRAS
A
>Mature_400_residues
ARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVVAALQDAAARDGAGATASHL
ICGHHTAHETLEREIADWLGYPSALLFGSGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEG
AMRQLKGAPEGAAMLATDGVFSMDGDVAPLRALSLVARMQHALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVTL
GKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRTRLVELIGAFRDGARKHGFELMAS
DTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPEGKARLRVTLSALHTPQQVQALIEAIVQARDVVSRQPLRASA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=369, Percent_Identity=36.5853658536585, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI284448556, Length=347, Percent_Identity=37.463976945245, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI83977442, Length=354, Percent_Identity=31.9209039548023, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI83977440, Length=354, Percent_Identity=31.9209039548023, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI83977444, Length=354, Percent_Identity=31.9209039548023, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI4502025, Length=405, Percent_Identity=29.6296296296296, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI40316939, Length=405, Percent_Identity=29.6296296296296, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=27.3972602739726, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI119220554, Length=362, Percent_Identity=25.6906077348066, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI5454084, Length=250, Percent_Identity=22.4, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786993, Length=354, Percent_Identity=43.2203389830509, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1790046, Length=352, Percent_Identity=35.5113636363636, Blast_Score=211, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI71994529, Length=368, Percent_Identity=36.4130434782609, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI32566772, Length=360, Percent_Identity=27.7777777777778, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560912, Length=385, Percent_Identity=25.974025974026, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17560914, Length=385, Percent_Identity=25.974025974026, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71982625, Length=283, Percent_Identity=23.321554770318, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71982617, Length=283, Percent_Identity=23.321554770318, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320438, Length=366, Percent_Identity=29.7814207650273, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320267, Length=372, Percent_Identity=22.8494623655914, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323954, Length=310, Percent_Identity=23.2258064516129, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24662918, Length=367, Percent_Identity=34.3324250681199, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137420, Length=396, Percent_Identity=30.3030303030303, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17136286, Length=381, Percent_Identity=26.246719160105, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24653280, Length=280, Percent_Identity=24.6428571428571, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653276, Length=280, Percent_Identity=24.6428571428571, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653278, Length=280, Percent_Identity=24.6428571428571, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 43176; Mature: 43045

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCHHHHH
VVAALQDAAARDGAGATASHLICGHHTAHETLEREIADWLGYPSALLFGSGFIANLAVQQ
HHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHH
ALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEGAMRQLKGAPEGAAMLATDG
HHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECC
VFSMDGDVAPLRALSLVARMQHALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCCCCCEECCCCCHHHHHHHHHH
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRTRL
HHHHHCCCCEEEECHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
VELIGAFRDGARKHGFELMASDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPE
HHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GKARLRVTLSALHTPQQVQALIEAIVQARDVVSRQPLRASA
CCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCHHHHH
VVAALQDAAARDGAGATASHLICGHHTAHETLEREIADWLGYPSALLFGSGFIANLAVQQ
HHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHH
ALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEGAMRQLKGAPEGAAMLATDG
HHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECC
VFSMDGDVAPLRALSLVARMQHALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCCCCCEECCCCCHHHHHHHHHH
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRTRL
HHHHHCCCCEEEECHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
VELIGAFRDGARKHGFELMASDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPE
HHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GKARLRVTLSALHTPQQVQALIEAIVQARDVVSRQPLRASA
CCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]