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The map label for this gene is pilD [H]

Identifier: 78045067

GI number: 78045067

Start: 549064

End: 549813

Strand: Direct

Name: pilD [H]

Synonym: CHY_0634

Alternate gene names: 78045067

Gene position: 549064-549813 (Clockwise)

Preceding gene: 78043168

Following gene: 78044377

Centisome position: 22.86

GC content: 39.2

Gene sequence:

>750_bases
GTGGAAGTTTTAATAATCTTTCTTTTTGGCCTGATAATTGGAAGCTTTTTAAATGTAGTTATCTATAGACTTCCCAGAGG
ACAATCAATACTGTATCCTCCATCGACCTGTCCAAACTGCGGTCAAAGACTAAAACCATGGCACTTAATTCCAGTATTAA
GCTTTCTCTTGCAAAAAGGTCGCTGTGCTTATTGCGGGGGGAAGATATCTTTTAAATACCCTTTGGTGGAGTTAATCACC
GCTTTTATCTTTAGCGTTGTGCACTTGAAATTTGGCTTAAGTTTATTAACTCTAAAATATCTACTTTTTTTTATTTTCCT
TATCCCGCTATTTTTTCTTGACCTGGAAAACTTTCTCCTGCCGGATAAGCTAACTTACCCTTTATTTCTGGCAGGAGTTT
TGATAAACCTTATTACCCGGGAGCTTTCCTGGAAAAGCTTAATTTTTGGCGTTATTACCGGTTTTGGCTTACTGTTCCTC
CTGGCTCTTATCTCCCGCGGAGGTATGGGCGGAGGGGATATTAAGCTTGCCGCAGGGCTCGGGGCATTTTTGGGTTTTCC
CCTTATTTTAGAGACGCTATTTTTAGCGTTTTTCTTTGGCGGGCTTACTGGGATAATTTTGCTTTTGACGAAAAAAAAGG
CTCGCGGTGACATGGTACCTTTTGGCCCGTTTTTAATAGGGGCAGCATTTATTACCGTTTTATGGGGAGAAAAAATTATA
AAATGGTATTTAAAAATATTTTTCCTGTAA

Upstream 100 bases:

>100_bases
CTGATGTTAATGGGGTTGATCAAGGTAAGAAGCCTTAATTTTTAAATATTTTTGGGCGGGGTTGTCCCGCCCTAATATCC
ATTTTTTTCGAGGTGAAGAA

Downstream 100 bases:

>100_bases
CCGAAAAAGGCTTTACTTTAATCGAGCTCATTGTGGTAATAACTATAGCCGGTATAATTTTTTTAACGGCGGCAATTAGC
TTAGATTATACTTTAGCAAA

Product: type IV prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MEVLIIFLFGLIIGSFLNVVIYRLPRGQSILYPPSTCPNCGQRLKPWHLIPVLSFLLQKGRCAYCGGKISFKYPLVELIT
AFIFSVVHLKFGLSLLTLKYLLFFIFLIPLFFLDLENFLLPDKLTYPLFLAGVLINLITRELSWKSLIFGVITGFGLLFL
LALISRGGMGGGDIKLAAGLGAFLGFPLILETLFLAFFFGGLTGIILLLTKKKARGDMVPFGPFLIGAAFITVLWGEKII
KWYLKIFFL

Sequences:

>Translated_249_residues
MEVLIIFLFGLIIGSFLNVVIYRLPRGQSILYPPSTCPNCGQRLKPWHLIPVLSFLLQKGRCAYCGGKISFKYPLVELIT
AFIFSVVHLKFGLSLLTLKYLLFFIFLIPLFFLDLENFLLPDKLTYPLFLAGVLINLITRELSWKSLIFGVITGFGLLFL
LALISRGGMGGGDIKLAAGLGAFLGFPLILETLFLAFFFGGLTGIILLLTKKKARGDMVPFGPFLIGAAFITVLWGEKII
KWYLKIFFL
>Mature_249_residues
MEVLIIFLFGLIIGSFLNVVIYRLPRGQSILYPPSTCPNCGQRLKPWHLIPVLSFLLQKGRCAYCGGKISFKYPLVELIT
AFIFSVVHLKFGLSLLTLKYLLFFIFLIPLFFLDLENFLLPDKLTYPLFLAGVLINLITRELSWKSLIFGVITGFGLLFL
LALISRGGMGGGDIKLAAGLGAFLGFPLILETLFLAFFFGGLTGIILLLTKKKARGDMVPFGPFLIGAAFITVLWGEKII
KWYLKIFFL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=240, Percent_Identity=35, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 27843; Mature: 27843

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVLIIFLFGLIIGSFLNVVIYRLPRGQSILYPPSTCPNCGQRLKPWHLIPVLSFLLQKG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHCC
RCAYCGGKISFKYPLVELITAFIFSVVHLKFGLSLLTLKYLLFFIFLIPLFFLDLENFLL
CEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDKLTYPLFLAGVLINLITRELSWKSLIFGVITGFGLLFLLALISRGGMGGGDIKLAAGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GAFLGFPLILETLFLAFFFGGLTGIILLLTKKKARGDMVPFGPFLIGAAFITVLWGEKII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KWYLKIFFL
HHHHHHHCC
>Mature Secondary Structure
MEVLIIFLFGLIIGSFLNVVIYRLPRGQSILYPPSTCPNCGQRLKPWHLIPVLSFLLQKG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHCC
RCAYCGGKISFKYPLVELITAFIFSVVHLKFGLSLLTLKYLLFFIFLIPLFFLDLENFLL
CEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDKLTYPLFLAGVLINLITRELSWKSLIFGVITGFGLLFLLALISRGGMGGGDIKLAAGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GAFLGFPLILETLFLAFFFGGLTGIILLLTKKKARGDMVPFGPFLIGAAFITVLWGEKII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KWYLKIFFL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]