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The map label for this gene is cobA [H]

Identifier: 78045040

GI number: 78045040

Start: 685897

End: 686670

Strand: Direct

Name: cobA [H]

Synonym: CHY_0778

Alternate gene names: 78045040

Gene position: 685897-686670 (Clockwise)

Preceding gene: 78044437

Following gene: 78044598

Centisome position: 28.56

GC content: 49.1

Gene sequence:

>774_bases
ATGACCAGGTTTGCAATGAACGGCAAGGTATATTTAGTAGGGGCGGGACCGGGAGACCCGGAGCTCATTACGGTAAAAGG
GATAAAACTTTTAAAAGAGGCTCAGGTGGTCGTTTACGACCGGCTGATAAGCCCCGAGCTTCTAAAATATGTAAATCCTT
CTGCTGAACTTTACTATGCCGGAAAAGCTCCTGGCCGACACAGTTTAAATCAGGAGGAGATAAACGAGCTTTTAATACAA
AAAGCCCGGGAAGGAAAAATGGTGGTACGGCTTAAAGGGGGAGATCCTTTTGTTTTTGGCCGGGGAGGGGAAGAAGCTTT
GGCTTTAACCCGGGCCGGGATTCCCTTTGAGGTGGTTCCGGGAGTTACTTCGGCGGTAGCGGTACCGGCGTATGCGGGTA
TTCCGGTAACTCACCGCCGGATTGCTTCAACTTTTACGGTAGTTACCGGCAATGAAACGGAAAATAAAGAAGAGCGGGAG
GTAGACTGGGAAAAAATTGCCCGGGTCGGGGGTACCATTGTCATTTTAATGGGATTAAGTAACCTTCCGTTTATAAAAGG
TGAACTTTTAAAATACTTGCCTGCCACCACACCGGTGGCGGTAATTTACCGGGGAACTACCCCCAGCCAGAAAATTGTCA
CCGGGACCCTGGAAGACATTGTAGAAAAGGTATTGGCGGCGGGAATCAAGCATCCGGCGGTAATTGTGGTGGGTGATGTG
GTAAAATTACAAGGGGAGCTTGGCTGGTATTCCCCATCAGCATTAACGGAGTGA

Upstream 100 bases:

>100_bases
TATGGCTGTCCCAAATACCGCCAGTTGGGGCTGGGGCAGTCTGAAAGGCGGACAACAAATACCCCAACCTTCTAAACTGA
TGCAGTTTTGCACAGTTTGG

Downstream 100 bases:

>100_bases
GCTTAAAATGTTAACTACCTTTCTTTTAGGCCTTACCTTTTTCACCAGGATTCCGGTGCCGGGAAAACTTAATTTTTCCG
AAGAAAAATTTAACAGGGCT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTRFAMNGKVYLVGAGPGDPELITVKGIKLLKEAQVVVYDRLISPELLKYVNPSAELYYAGKAPGRHSLNQEEINELLIQ
KAREGKMVVRLKGGDPFVFGRGGEEALALTRAGIPFEVVPGVTSAVAVPAYAGIPVTHRRIASTFTVVTGNETENKEERE
VDWEKIARVGGTIVILMGLSNLPFIKGELLKYLPATTPVAVIYRGTTPSQKIVTGTLEDIVEKVLAAGIKHPAVIVVGDV
VKLQGELGWYSPSALTE

Sequences:

>Translated_257_residues
MTRFAMNGKVYLVGAGPGDPELITVKGIKLLKEAQVVVYDRLISPELLKYVNPSAELYYAGKAPGRHSLNQEEINELLIQ
KAREGKMVVRLKGGDPFVFGRGGEEALALTRAGIPFEVVPGVTSAVAVPAYAGIPVTHRRIASTFTVVTGNETENKEERE
VDWEKIARVGGTIVILMGLSNLPFIKGELLKYLPATTPVAVIYRGTTPSQKIVTGTLEDIVEKVLAAGIKHPAVIVVGDV
VKLQGELGWYSPSALTE
>Mature_256_residues
TRFAMNGKVYLVGAGPGDPELITVKGIKLLKEAQVVVYDRLISPELLKYVNPSAELYYAGKAPGRHSLNQEEINELLIQK
AREGKMVVRLKGGDPFVFGRGGEEALALTRAGIPFEVVPGVTSAVAVPAYAGIPVTHRRIASTFTVVTGNETENKEEREV
DWEKIARVGGTIVILMGLSNLPFIKGELLKYLPATTPVAVIYRGTTPSQKIVTGTLEDIVEKVLAAGIKHPAVIVVGDVV
KLQGELGWYSPSALTE

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=245, Percent_Identity=46.1224489795918, Blast_Score=226, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=33.8983050847458, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27689; Mature: 27557

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFAMNGKVYLVGAGPGDPELITVKGIKLLKEAQVVVYDRLISPELLKYVNPSAELYYA
CCEEEECCEEEEEECCCCCCCEEEECCHHHHHHHHHEEEHHHCCHHHHHHCCCCCEEEEE
GKAPGRHSLNQEEINELLIQKAREGKMVVRLKGGDPFVFGRGGEEALALTRAGIPFEVVP
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCCEEECC
GVTSAVAVPAYAGIPVTHRRIASTFTVVTGNETENKEEREVDWEKIARVGGTIVILMGLS
CCHHHHHCCCCCCCCHHHHHHHEEEEEEECCCCCCCHHHCCCHHHHHHCCCEEEEEEECC
NLPFIKGELLKYLPATTPVAVIYRGTTPSQKIVTGTLEDIVEKVLAAGIKHPAVIVVGDV
CCCCHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEECE
VKLQGELGWYSPSALTE
EEEECCCCEECCHHCCC
>Mature Secondary Structure 
TRFAMNGKVYLVGAGPGDPELITVKGIKLLKEAQVVVYDRLISPELLKYVNPSAELYYA
CEEEECCEEEEEECCCCCCCEEEECCHHHHHHHHHEEEHHHCCHHHHHHCCCCCEEEEE
GKAPGRHSLNQEEINELLIQKAREGKMVVRLKGGDPFVFGRGGEEALALTRAGIPFEVVP
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCCEEECC
GVTSAVAVPAYAGIPVTHRRIASTFTVVTGNETENKEEREVDWEKIARVGGTIVILMGLS
CCHHHHHCCCCCCCCHHHHHHHEEEEEEECCCCCCCHHHCCCHHHHHHCCCEEEEEEECC
NLPFIKGELLKYLPATTPVAVIYRGTTPSQKIVTGTLEDIVEKVLAAGIKHPAVIVVGDV
CCCCHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEECE
VKLQGELGWYSPSALTE
EEEECCCCEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]