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The map label for this gene is ispE

Identifier: 78044899

GI number: 78044899

Start: 156534

End: 157400

Strand: Direct

Name: ispE

Synonym: CHY_0188

Alternate gene names: 78044899

Gene position: 156534-157400 (Clockwise)

Preceding gene: 78043003

Following gene: 78045171

Centisome position: 6.52

GC content: 39.68

Gene sequence:

>867_bases
TTGTTAATTTTTGCGCCGGCTAAAATAAATCTCACTTTAGATATATTAGGAAAACGTCCCGATGGTTACCACGAACTCTG
GTCGGTAATGCAGGCAATTACTCTGGGAGATTTAGTGGAAATTGAACCGGCAGAGGGAATAAACTTAAGGGTTGTAGGGG
CAGATTTACCTGTGGATTCTACCAATATTGCGTTTAAAGCTGTTCAGGCTTTAAGGGCCGCCACTGGGAAAGCCATAGGA
GCCAGCATTACTATCCGTAAAAAAATCCCCTTAGAAGCGGGACTGGCCGGTGGCTCGGCGGATGGAGCCGCAGTTCTTTA
TGGTTTAAATAAACTTTATAATTTAAATTTAAGTCAGGAAGAGCTTTTAGAAATTGGGGCAAAAATAAGCGCCGATATTC
CCTTTTGCCTTACCGGTGGCACCGCCCTGGTGCAGGGGATAGGGGAAAAAGTAAAAAAACTTCCGCCCTTAAAAAAAGGT
TATTTTGTTATCTATAAACCCCCTTTTGGGATCTCGACAAAAGAAGCTTATTTAAGGCTTGCGGGTAAGGATTTAACAAA
GGAGCATCCTGATCGCGAAAAAATCCTGAAAGCTTTAGGCAAAGAAAATTTAGAGGATTTGGGAAAGTTTTTAAAGAATC
TTTTAGAAATAAGTGCTTTGGAAATAAATCCGGAAATATATAAATATAAAAACGAACTTTTAAATTTAAAACCTTTAGGG
GTGCTAATGTCGGGTAGCGGTTCTGCTCTTTTTGCTTTAACGGAAAATTTAAAAAAAGCGAAGGAAATTTATTATCAATT
GACATTGCCTGGACAAAAATTTATTGTCCGCCCGTATGCGGCCGGTCCTACTTGCTTAAAGCTTTAA

Upstream 100 bases:

>100_bases
GTTGTTTTTATAGAGGGTTAGAAGCTTCTTTTTTAGACTGACGGTTAAATGGACTATCATTACTTTCGCCTCCTTTCCGG
GGTGATGGGGAGGAAAAAAG

Downstream 100 bases:

>100_bases
CTCCATAGTAAAATATAATAATACAAATTATCGTAGAAATGAGGGGTTAAAATGGGGAGATTAAAACCTATTATCCTTGA
TAATTACAAGCCTTTAAGGG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MLIFAPAKINLTLDILGKRPDGYHELWSVMQAITLGDLVEIEPAEGINLRVVGADLPVDSTNIAFKAVQALRAATGKAIG
ASITIRKKIPLEAGLAGGSADGAAVLYGLNKLYNLNLSQEELLEIGAKISADIPFCLTGGTALVQGIGEKVKKLPPLKKG
YFVIYKPPFGISTKEAYLRLAGKDLTKEHPDREKILKALGKENLEDLGKFLKNLLEISALEINPEIYKYKNELLNLKPLG
VLMSGSGSALFALTENLKKAKEIYYQLTLPGQKFIVRPYAAGPTCLKL

Sequences:

>Translated_288_residues
MLIFAPAKINLTLDILGKRPDGYHELWSVMQAITLGDLVEIEPAEGINLRVVGADLPVDSTNIAFKAVQALRAATGKAIG
ASITIRKKIPLEAGLAGGSADGAAVLYGLNKLYNLNLSQEELLEIGAKISADIPFCLTGGTALVQGIGEKVKKLPPLKKG
YFVIYKPPFGISTKEAYLRLAGKDLTKEHPDREKILKALGKENLEDLGKFLKNLLEISALEINPEIYKYKNELLNLKPLG
VLMSGSGSALFALTENLKKAKEIYYQLTLPGQKFIVRPYAAGPTCLKL
>Mature_288_residues
MLIFAPAKINLTLDILGKRPDGYHELWSVMQAITLGDLVEIEPAEGINLRVVGADLPVDSTNIAFKAVQALRAATGKAIG
ASITIRKKIPLEAGLAGGSADGAAVLYGLNKLYNLNLSQEELLEIGAKISADIPFCLTGGTALVQGIGEKVKKLPPLKKG
YFVIYKPPFGISTKEAYLRLAGKDLTKEHPDREKILKALGKENLEDLGKFLKNLLEISALEINPEIYKYKNELLNLKPLG
VLMSGSGSALFALTENLKKAKEIYYQLTLPGQKFIVRPYAAGPTCLKL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=276, Percent_Identity=30.7971014492754, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CARHZ (Q3AFM4)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359060.1
- ProteinModelPortal:   Q3AFM4
- SMR:   Q3AFM4
- STRING:   Q3AFM4
- GeneID:   3728024
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0188
- NMPDR:   fig|246194.3.peg.1007
- TIGR:   CHY_0188
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- BioCyc:   CHYD246194:CHY_0188-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31185; Mature: 31185

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIFAPAKINLTLDILGKRPDGYHELWSVMQAITLGDLVEIEPAEGINLRVVGADLPVDS
CEEEECEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCC
TNIAFKAVQALRAATGKAIGASITIRKKIPLEAGLAGGSADGAAVLYGLNKLYNLNLSQE
CHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCCHHEEEECHHHEECCCCHH
ELLEIGAKISADIPFCLTGGTALVQGIGEKVKKLPPLKKGYFVIYKPPFGISTKEAYLRL
HHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHEE
AGKDLTKEHPDREKILKALGKENLEDLGKFLKNLLEISALEINPEIYKYKNELLNLKPLG
CCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCEE
VLMSGSGSALFALTENLKKAKEIYYQLTLPGQKFIVRPYAAGPTCLKL
EEEECCCCEEEHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCEECC
>Mature Secondary Structure
MLIFAPAKINLTLDILGKRPDGYHELWSVMQAITLGDLVEIEPAEGINLRVVGADLPVDS
CEEEECEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCC
TNIAFKAVQALRAATGKAIGASITIRKKIPLEAGLAGGSADGAAVLYGLNKLYNLNLSQE
CHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCCHHEEEECHHHEECCCCHH
ELLEIGAKISADIPFCLTGGTALVQGIGEKVKKLPPLKKGYFVIYKPPFGISTKEAYLRL
HHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHEE
AGKDLTKEHPDREKILKALGKENLEDLGKFLKNLLEISALEINPEIYKYKNELLNLKPLG
CCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCEE
VLMSGSGSALFALTENLKKAKEIYYQLTLPGQKFIVRPYAAGPTCLKL
EEEECCCCEEEHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA