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The map label for this gene is grpE

Identifier: 78044836

GI number: 78044836

Start: 357288

End: 357872

Strand: Direct

Name: grpE

Synonym: CHY_0414

Alternate gene names: 78044836

Gene position: 357288-357872 (Clockwise)

Preceding gene: 78043200

Following gene: 78045047

Centisome position: 14.88

GC content: 38.46

Gene sequence:

>585_bases
ATGGAGGAAAAAGATAAAGAGGAAAAAGTAACCGGAGAAAATTTGGAACCGGAAGATAAAAATTTGGAACAGGAAGATAA
AGAGGAGGTTGTCGGGCCCCAGGAGGAACAACAAATTGATGAGGCCAAAAATTGGGAAGAAGAATACAATAAGCTTTTGG
ATGAACATAACCGCTTAAAAAATCAGTATTTAAGGCTATATGCCGATTTCGATAATTACCGGAAAAGAACCCAGAGAGAA
AAAGAGGAGCTTTTAAAGTACGAGGGAATGGAGTTTCTAAAAAAGCTCCTGCCGGTGCTTGATAATTTTGAAAGGGCTTT
AAAGGAAAAAGACACTGATCCGCAAAAAGTGATTGAAGGAGTTGAGCTTACGCACCGGCAGTTACTGGAAATATTAAACC
AGCATGAAGTAAAAGCTATAGAAGCTCAAGGTCAGCCGTTTAATCCCGAACTCCACGAAGCTTTAATGGTGGAGGTACGG
GAGGATTTAGAAGAAAATACCGTAATTGAGGAGCTGGTAAAAGGGTATTTTTATAAAGATAAGGTTTTAAGACCTGCTTT
GGTAAAAGTTTCGAAAAAACAATAA

Upstream 100 bases:

>100_bases
GCAGTAAAAAATGCCAGCGAGGTAGCTATTTCGTTATTGAAAATTAATACCATAATTAAAATGAAAGATTACGAAGTTTT
AAAGGAAGGGGAGCAGGAAG

Downstream 100 bases:

>100_bases
CTTAAAGGGGGTAGAGATAATGGGTAGAATTGTTGGTATTGACCTTGGAACTACAAACTCCTGTATTGCGGTAATGGAAG
GTGGAGAAGTAATCGTTATT

Product: grpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MEEKDKEEKVTGENLEPEDKNLEQEDKEEVVGPQEEQQIDEAKNWEEEYNKLLDEHNRLKNQYLRLYADFDNYRKRTQRE
KEELLKYEGMEFLKKLLPVLDNFERALKEKDTDPQKVIEGVELTHRQLLEILNQHEVKAIEAQGQPFNPELHEALMVEVR
EDLEENTVIEELVKGYFYKDKVLRPALVKVSKKQ

Sequences:

>Translated_194_residues
MEEKDKEEKVTGENLEPEDKNLEQEDKEEVVGPQEEQQIDEAKNWEEEYNKLLDEHNRLKNQYLRLYADFDNYRKRTQRE
KEELLKYEGMEFLKKLLPVLDNFERALKEKDTDPQKVIEGVELTHRQLLEILNQHEVKAIEAQGQPFNPELHEALMVEVR
EDLEENTVIEELVKGYFYKDKVLRPALVKVSKKQ
>Mature_194_residues
MEEKDKEEKVTGENLEPEDKNLEQEDKEEVVGPQEEQQIDEAKNWEEEYNKLLDEHNRLKNQYLRLYADFDNYRKRTQRE
KEELLKYEGMEFLKKLLPVLDNFERALKEKDTDPQKVIEGVELTHRQLLEILNQHEVKAIEAQGQPFNPELHEALMVEVR
EDLEENTVIEELVKGYFYKDKVLRPALVKVSKKQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=31.875, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653432, Length=141, Percent_Identity=31.2056737588652, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CARHZ (Q3AF09)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359275.1
- ProteinModelPortal:   Q3AF09
- SMR:   Q3AF09
- STRING:   Q3AF09
- GeneID:   3727961
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0414
- NMPDR:   fig|246194.3.peg.747
- TIGR:   CHY_0414
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   LMVANDE
- BioCyc:   CHYD246194:CHY_0414-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 23240; Mature: 23240

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKDKEEKVTGENLEPEDKNLEQEDKEEVVGPQEEQQIDEAKNWEEEYNKLLDEHNRLK
CCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
NQYLRLYADFDNYRKRTQREKEELLKYEGMEFLKKLLPVLDNFERALKEKDTDPQKVIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VELTHRQLLEILNQHEVKAIEAQGQPFNPELHEALMVEVREDLEENTVIEELVKGYFYKD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVLRPALVKVSKKQ
HHHHHHHHHHCCCC
>Mature Secondary Structure
MEEKDKEEKVTGENLEPEDKNLEQEDKEEVVGPQEEQQIDEAKNWEEEYNKLLDEHNRLK
CCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
NQYLRLYADFDNYRKRTQREKEELLKYEGMEFLKKLLPVLDNFERALKEKDTDPQKVIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VELTHRQLLEILNQHEVKAIEAQGQPFNPELHEALMVEVREDLEENTVIEELVKGYFYKD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVLRPALVKVSKKQ
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA