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The map label for this gene is tig

Identifier: 78044834

GI number: 78044834

Start: 279138

End: 280421

Strand: Direct

Name: tig

Synonym: CHY_0324

Alternate gene names: 78044834

Gene position: 279138-280421 (Clockwise)

Preceding gene: 78044258

Following gene: 78043074

Centisome position: 11.62

GC content: 40.97

Gene sequence:

>1284_bases
ATGAAAGTTACCAAAGAAACTTTAGAAAAGAGTCGGGTGGAGTTAACCATCGAGGTAGAGGCGGAGGAAGTTTCTAAAGC
ATACGAAAAAGCTTATAAAAAGATTGCTCAAAAAGTGGTTATCCCTGGTTTTAGAAAAGGAAAGGCTCCCAGGGTTTTAG
TGGAAAGACATGTGGGTAAAGAGTATATTTTAGAAGAAGCCCTGGATGAACTGTTGCCGGAGAGCTATGTTAAAGCGGTT
AATGAGGCAGGAATCGAACCGGTAGATAAGCCGGAAGTATCGTTAGTTTCCTACGGGGTTAACGAACCGCTGGTTTATAA
AGCGGTAGTGGATGTGAAGCCCGAGGTTGAGCTTGGTCAATATACCGGCTTGGAAGTAACCAAAATGCCCGTTGAAGTTA
CCGACGAAGAAGTAGAAAAAGAACTGGAATATCTTCAGAATCGGTATGCGAAACTTATTACCGTTGAAGATGGTGAAGCA
AAATTTGGTGATATTGTGGTAATTGACTTTGCTGGGAAAATGAATGGAGAACCTCTCGAAGGGGGTAGTGCGGACAATTA
CCGGTTAGAACTGGGCTCAAAGGTTTTTATTCCTGGCTTTGAAGAGCAAATTGTGGGTATGAAACCGGGAGAAACAAAGG
AAATTAACGTTACCTTCCCGGAAGATTACCAGAAAGAAGACCTTGCTGGGAAACCGGCAGTCTTTACTGTAACCTTAAAA
GAAATTAAACGGAAAGAGTTAGCGCCCCTTGATGATGAGTTTGCCAAAGATGTTTCCGAATTTTCAACTTTGGCGGAGTT
AAAAGAAGACCTCAAGAAAAAGATTGCTCAAACCAAGGAAAATATTTCCCGGGAAAAAATGGAAGCAGAAGTAGTTGAAA
AAGCGGTAGATAATGCTAATGTGGAAATTCCCGCAAGCATGGTAAATCATGAAGTTGAGCATATACTTCATCATTTTGAG
GAAGAGCTAAAGTACCGGCGGTTAACCTTGGAACAGTACCTGAATTACCAGAAGAAAACCCTGGATGAATTAAAGGAAGA
ATTAAAACCAAGAGCCGAACGCAATGTTAAAACCGAACTGGTTTTAGAAGCTATTGCCAAAAAAGAAGGAATTACCGCTA
CCGATGAAGAAATAGATAAGGAACTTGGTAAAATCGCCGAACTTTACCAGCAACCGGTAGAGGAAATTAAAAAGCTTTTT
GCTCAGCGGATGGATGATCTTGCCTATTCCATTGTTCGCCGAAAAACGATTGACTTTTTGGTTGAAAATGCTAAAGCTGT
TTAA

Upstream 100 bases:

>100_bases
GTAGAATATAATAGTAATTAAATATATTGTTGCAACGGGATATGCCAAAAAAAGGAAAAGGTGACAACAGATTAAAATAC
ATATTGCAAGGAGGATTAAG

Downstream 100 bases:

>100_bases
TTAAACAGATAAGGGGGTTTGGTTATGAGCTATCTTGTTCCCATTGTAATAGAACAAACCAGCCGGGGAGAAAGAAGTTA
CGATATTTGGTCAAGGTTAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKVTKETLEKSRVELTIEVEAEEVSKAYEKAYKKIAQKVVIPGFRKGKAPRVLVERHVGKEYILEEALDELLPESYVKAV
NEAGIEPVDKPEVSLVSYGVNEPLVYKAVVDVKPEVELGQYTGLEVTKMPVEVTDEEVEKELEYLQNRYAKLITVEDGEA
KFGDIVVIDFAGKMNGEPLEGGSADNYRLELGSKVFIPGFEEQIVGMKPGETKEINVTFPEDYQKEDLAGKPAVFTVTLK
EIKRKELAPLDDEFAKDVSEFSTLAELKEDLKKKIAQTKENISREKMEAEVVEKAVDNANVEIPASMVNHEVEHILHHFE
EELKYRRLTLEQYLNYQKKTLDELKEELKPRAERNVKTELVLEAIAKKEGITATDEEIDKELGKIAELYQQPVEEIKKLF
AQRMDDLAYSIVRRKTIDFLVENAKAV

Sequences:

>Translated_427_residues
MKVTKETLEKSRVELTIEVEAEEVSKAYEKAYKKIAQKVVIPGFRKGKAPRVLVERHVGKEYILEEALDELLPESYVKAV
NEAGIEPVDKPEVSLVSYGVNEPLVYKAVVDVKPEVELGQYTGLEVTKMPVEVTDEEVEKELEYLQNRYAKLITVEDGEA
KFGDIVVIDFAGKMNGEPLEGGSADNYRLELGSKVFIPGFEEQIVGMKPGETKEINVTFPEDYQKEDLAGKPAVFTVTLK
EIKRKELAPLDDEFAKDVSEFSTLAELKEDLKKKIAQTKENISREKMEAEVVEKAVDNANVEIPASMVNHEVEHILHHFE
EELKYRRLTLEQYLNYQKKTLDELKEELKPRAERNVKTELVLEAIAKKEGITATDEEIDKELGKIAELYQQPVEEIKKLF
AQRMDDLAYSIVRRKTIDFLVENAKAV
>Mature_427_residues
MKVTKETLEKSRVELTIEVEAEEVSKAYEKAYKKIAQKVVIPGFRKGKAPRVLVERHVGKEYILEEALDELLPESYVKAV
NEAGIEPVDKPEVSLVSYGVNEPLVYKAVVDVKPEVELGQYTGLEVTKMPVEVTDEEVEKELEYLQNRYAKLITVEDGEA
KFGDIVVIDFAGKMNGEPLEGGSADNYRLELGSKVFIPGFEEQIVGMKPGETKEINVTFPEDYQKEDLAGKPAVFTVTLK
EIKRKELAPLDDEFAKDVSEFSTLAELKEDLKKKIAQTKENISREKMEAEVVEKAVDNANVEIPASMVNHEVEHILHHFE
EELKYRRLTLEQYLNYQKKTLDELKEELKPRAERNVKTELVLEAIAKKEGITATDEEIDKELGKIAELYQQPVEEIKKLF
AQRMDDLAYSIVRRKTIDFLVENAKAV

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=397, Percent_Identity=30.4785894206549, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CARHZ (Q3AF97)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359187.1
- HSSP:   Q9RT21
- ProteinModelPortal:   Q3AF97
- STRING:   Q3AF97
- GeneID:   3727959
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0324
- NMPDR:   fig|246194.3.peg.405
- TIGR:   CHY_0324
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   CHYD246194:CHY_0324-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48608; Mature: 48608

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTKETLEKSRVELTIEVEAEEVSKAYEKAYKKIAQKVVIPGFRKGKAPRVLVERHVGK
CCCCHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCH
EYILEEALDELLPESYVKAVNEAGIEPVDKPEVSLVSYGVNEPLVYKAVVDVKPEVELGQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCEEEEEEECCCCCCCCCC
YTGLEVTKMPVEVTDEEVEKELEYLQNRYAKLITVEDGEAKFGDIVVIDFAGKMNGEPLE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCCCC
GGSADNYRLELGSKVFIPGFEEQIVGMKPGETKEINVTFPEDYQKEDLAGKPAVFTVTLK
CCCCCCEEEEECCEEECCCHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCEEEEEEHH
EIKRKELAPLDDEFAKDVSEFSTLAELKEDLKKKIAQTKENISREKMEAEVVEKAVDNAN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEIPASMVNHEVEHILHHFEEELKYRRLTLEQYLNYQKKTLDELKEELKPRAERNVKTEL
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCHHHHH
VLEAIAKKEGITATDEEIDKELGKIAELYQQPVEEIKKLFAQRMDDLAYSIVRRKTIDFL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VENAKAV
HHHCCCC
>Mature Secondary Structure
MKVTKETLEKSRVELTIEVEAEEVSKAYEKAYKKIAQKVVIPGFRKGKAPRVLVERHVGK
CCCCHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCH
EYILEEALDELLPESYVKAVNEAGIEPVDKPEVSLVSYGVNEPLVYKAVVDVKPEVELGQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCEEEEEEECCCCCCCCCC
YTGLEVTKMPVEVTDEEVEKELEYLQNRYAKLITVEDGEAKFGDIVVIDFAGKMNGEPLE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCCCC
GGSADNYRLELGSKVFIPGFEEQIVGMKPGETKEINVTFPEDYQKEDLAGKPAVFTVTLK
CCCCCCEEEEECCEEECCCHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCEEEEEEHH
EIKRKELAPLDDEFAKDVSEFSTLAELKEDLKKKIAQTKENISREKMEAEVVEKAVDNAN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEIPASMVNHEVEHILHHFEEELKYRRLTLEQYLNYQKKTLDELKEELKPRAERNVKTEL
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCHHHHH
VLEAIAKKEGITATDEEIDKELGKIAELYQQPVEEIKKLFAQRMDDLAYSIVRRKTIDFL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VENAKAV
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA