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The map label for this gene is thiE [H]

Identifier: 78044820

GI number: 78044820

Start: 656949

End: 658043

Strand: Direct

Name: thiE [H]

Synonym: CHY_0746

Alternate gene names: 78044820

Gene position: 656949-658043 (Clockwise)

Preceding gene: 78042802

Following gene: 78043716

Centisome position: 27.36

GC content: 45.75

Gene sequence:

>1095_bases
ATGATAAGGTACAACCAACCTCTTCCATCTTCTGGAAGGGGTTTATTTTTTTCGGGGAGGTATTTGATGGAAATTTACCG
GATTATTGATGTTAATTTAAACCGGGCCCGGGAAGGAATCCGGGTGCTGGAGGAAGTAGCTCGCTTTGGTTTGGCTAACA
GAAAATTATTTGAGGAATTTAAACATTTGCGACATTTTATCAAAGAAATAGAAAAAAGTCTTGCTGGAAATCCCTTGTGG
TATCGGGATGCTCAAAACGACCCGGGACAGGAACTAAGCTCAGAGGAGATGGCGAGGAATAATTTGCGGGAGGTGGTGTT
AGCCAACGCCAAAAGAGCCCAGGAAAGTCTGAGGGTTTTAGAAGAATTTGGGAAGCTGTTAGATGCTGCGATTGTCCTTA
ATGCCAAAAAAGCAAGGTTTTTACTTTATGAGCTGGAAAAAGAAGTTTTAACCTTGATTAAGCCTTCGGTGGATTTAACC
TTATACGTACTTACCGATGATGCTTACCTTAACGAAGAAAAGTTCTGGAGGGTTGTCGAAGATTGCCTGAAAAATGGAGT
GACGGCTTTCCAGTACCGGGCAAAAGGAAAAAAGGGAGCCGAAATGTACCGGGAGGGGCTAAGGCTGAAAGAGCTTTGTG
CAAAATATGGGGTTTCGTTTTTTGTAAACGACCGGCTGGACCTGGGTCTGGCTTTAAATGCCGATGGGGTACATCTGGGA
CAGGAGGATTTACTCCTGGAGGTGGCAAGAAAGCATTTTCCCGGAAAAATTATCGGGCTTTCGGCTACCAATTATGAAGA
AGGGGTTTTAGGAATAAAGGCGGGAGCCGATTATCTTGGGCTTGGGCCAATTTTTCCCACGTCCACCAAGGAAGATGCCG
CACCGCCCTGCGGGGTAGAAGTGATTCAGAAGCTTAAAGAGGAGTTCCCCAATTCTCCGGTAATAGCTATTGGGGGTATT
GATCGGGAAAAGGTTTTTGAGGTTATTCGTGCCGGGGCCGATGGGATTGCGGTAATTTCGGCTGTATTTGGAGCGGAAAG
TCCGGCTCGGGCAGTTTATGAACTACGGGAAACAATAATTAGAGCAAGGGAGTGA

Upstream 100 bases:

>100_bases
CCTCAGGGGTGCCGGATGAGCCCGGCTGAGAAGGCTTCGCGATGAAGAAGTCTACCCTTTGAACCTGCTCTGGGTAATGC
CAGCGGAGGGAGAGGAAATA

Downstream 100 bases:

>100_bases
GCGATAATGACTCAGCTTTTAAAAGCAAAAGAGGGAGTTATTACCAGGGAAATGGAAGTAGTGGCGGCGGAGGAAAGAAA
ATCGCCGGAAGAAATCCGGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MIRYNQPLPSSGRGLFFSGRYLMEIYRIIDVNLNRAREGIRVLEEVARFGLANRKLFEEFKHLRHFIKEIEKSLAGNPLW
YRDAQNDPGQELSSEEMARNNLREVVLANAKRAQESLRVLEEFGKLLDAAIVLNAKKARFLLYELEKEVLTLIKPSVDLT
LYVLTDDAYLNEEKFWRVVEDCLKNGVTAFQYRAKGKKGAEMYREGLRLKELCAKYGVSFFVNDRLDLGLALNADGVHLG
QEDLLLEVARKHFPGKIIGLSATNYEEGVLGIKAGADYLGLGPIFPTSTKEDAAPPCGVEVIQKLKEEFPNSPVIAIGGI
DREKVFEVIRAGADGIAVISAVFGAESPARAVYELRETIIRARE

Sequences:

>Translated_364_residues
MIRYNQPLPSSGRGLFFSGRYLMEIYRIIDVNLNRAREGIRVLEEVARFGLANRKLFEEFKHLRHFIKEIEKSLAGNPLW
YRDAQNDPGQELSSEEMARNNLREVVLANAKRAQESLRVLEEFGKLLDAAIVLNAKKARFLLYELEKEVLTLIKPSVDLT
LYVLTDDAYLNEEKFWRVVEDCLKNGVTAFQYRAKGKKGAEMYREGLRLKELCAKYGVSFFVNDRLDLGLALNADGVHLG
QEDLLLEVARKHFPGKIIGLSATNYEEGVLGIKAGADYLGLGPIFPTSTKEDAAPPCGVEVIQKLKEEFPNSPVIAIGGI
DREKVFEVIRAGADGIAVISAVFGAESPARAVYELRETIIRARE
>Mature_364_residues
MIRYNQPLPSSGRGLFFSGRYLMEIYRIIDVNLNRAREGIRVLEEVARFGLANRKLFEEFKHLRHFIKEIEKSLAGNPLW
YRDAQNDPGQELSSEEMARNNLREVVLANAKRAQESLRVLEEFGKLLDAAIVLNAKKARFLLYELEKEVLTLIKPSVDLT
LYVLTDDAYLNEEKFWRVVEDCLKNGVTAFQYRAKGKKGAEMYREGLRLKELCAKYGVSFFVNDRLDLGLALNADGVHLG
QEDLLLEVARKHFPGKIIGLSATNYEEGVLGIKAGADYLGLGPIFPTSTKEDAAPPCGVEVIQKLKEEFPNSPVIAIGGI
DREKVFEVIRAGADGIAVISAVFGAESPARAVYELRETIIRARE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=180, Percent_Identity=32.7777777777778, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=226, Percent_Identity=34.070796460177, Blast_Score=112, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 40909; Mature: 40909

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYNQPLPSSGRGLFFSGRYLMEIYRIIDVNLNRAREGIRVLEEVARFGLANRKLFEEF
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KHLRHFIKEIEKSLAGNPLWYRDAQNDPGQELSSEEMARNNLREVVLANAKRAQESLRVL
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEFGKLLDAAIVLNAKKARFLLYELEKEVLTLIKPSVDLTLYVLTDDAYLNEEKFWRVVE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHH
DCLKNGVTAFQYRAKGKKGAEMYREGLRLKELCAKYGVSFFVNDRLDLGLALNADGVHLG
HHHHCCCEEEHHHHCCCCHHHHHHHCCHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCC
QEDLLLEVARKHFPGKIIGLSATNYEEGVLGIKAGADYLGLGPIFPTSTKEDAAPPCGVE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
VIQKLKEEFPNSPVIAIGGIDREKVFEVIRAGADGIAVISAVFGAESPARAVYELRETII
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
RARE
HCCC
>Mature Secondary Structure
MIRYNQPLPSSGRGLFFSGRYLMEIYRIIDVNLNRAREGIRVLEEVARFGLANRKLFEEF
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KHLRHFIKEIEKSLAGNPLWYRDAQNDPGQELSSEEMARNNLREVVLANAKRAQESLRVL
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEFGKLLDAAIVLNAKKARFLLYELEKEVLTLIKPSVDLTLYVLTDDAYLNEEKFWRVVE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHH
DCLKNGVTAFQYRAKGKKGAEMYREGLRLKELCAKYGVSFFVNDRLDLGLALNADGVHLG
HHHHCCCEEEHHHHCCCCHHHHHHHCCHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCC
QEDLLLEVARKHFPGKIIGLSATNYEEGVLGIKAGADYLGLGPIFPTSTKEDAAPPCGVE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
VIQKLKEEFPNSPVIAIGGIDREKVFEVIRAGADGIAVISAVFGAESPARAVYELRETII
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
RARE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]