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The map label for this gene is lutR [H]

Identifier: 78044814

GI number: 78044814

Start: 376765

End: 377460

Strand: Direct

Name: lutR [H]

Synonym: CHY_0434

Alternate gene names: 78044814

Gene position: 376765-377460 (Clockwise)

Preceding gene: 78042735

Following gene: 78044955

Centisome position: 15.69

GC content: 40.09

Gene sequence:

>696_bases
ATGAATTTTCAGCAGATAAAAACCAAAAAAATTTACGAAGAAATAATCGAGCAAATTAGGTTGCTAATTGCCCGGGGTGA
ATTAAAGCCAGGGGATAAACTTCCGTCAGAAAGAGAACTTGCCGAAAGCCTTGGGGTCAGTAGAGCTTCGGTTAGAGAGG
CATTAAGTGCGTTAGAAGTATTAGGGGTATTAGAAGTAAGACCGGGGGAGGGTACTTTTGTCCGGGAAGTGGCCGCGGAT
AAGTCCTTTCATTCCCTAACTCTGTTGTTACTGTTAGGTACAACTTTGGAAGTTTTAGAAGTAAGAAAGATTTTAGAATC
CGGAGCAGTAGTACTGGCTACAGAAAGAGCTACCGATGAAGATATAAAACGCTTAGAAGATGCCGTAGTAAAAATGGAAA
AAGATTTAAAGCAGGGTGATTTAGGGGATGAGGCTGATTTTATGTTTCATTACGGATTGGCTCTTGCTACCCATAATTCC
CTTTTGGTACGTCTTATGAATTCCATTGCCGACACCATGCGCCAGTCTTTAAAAATAAACCGTGAGCGGCTTTTTAGAAC
TCCGGGCATGCCTGAAATTTTTTATAAGTACCACAAAGAAATTTTAGAGGCGATTAAAGCTCGCGATAAAATTAGAGCGC
AAAAAGTTTTAGAGGAACATTTAACGGTGGCTGAAGAAAAATTGTTAGAAGAGTAA

Upstream 100 bases:

>100_bases
GGCGGAAGGATATAAAGAAAATTAATTCACCCCTTAGAAGGATTTAACGGAAAAAGGGAGAAAATATAAAGGCAAAAATT
ACTTTTGCCGGAGGTTTCGC

Downstream 100 bases:

>100_bases
TGGATTGAAAATAGTTTGATTTTAAAAAAGGGAAAATATAAAATCTAAGGTCAGACCATCAGGACATAATACCGAAGGTG
GTGATTGGATGTTAAAGGAA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNFQQIKTKKIYEEIIEQIRLLIARGELKPGDKLPSERELAESLGVSRASVREALSALEVLGVLEVRPGEGTFVREVAAD
KSFHSLTLLLLLGTTLEVLEVRKILESGAVVLATERATDEDIKRLEDAVVKMEKDLKQGDLGDEADFMFHYGLALATHNS
LLVRLMNSIADTMRQSLKINRERLFRTPGMPEIFYKYHKEILEAIKARDKIRAQKVLEEHLTVAEEKLLEE

Sequences:

>Translated_231_residues
MNFQQIKTKKIYEEIIEQIRLLIARGELKPGDKLPSERELAESLGVSRASVREALSALEVLGVLEVRPGEGTFVREVAAD
KSFHSLTLLLLLGTTLEVLEVRKILESGAVVLATERATDEDIKRLEDAVVKMEKDLKQGDLGDEADFMFHYGLALATHNS
LLVRLMNSIADTMRQSLKINRERLFRTPGMPEIFYKYHKEILEAIKARDKIRAQKVLEEHLTVAEEKLLEE
>Mature_231_residues
MNFQQIKTKKIYEEIIEQIRLLIARGELKPGDKLPSERELAESLGVSRASVREALSALEVLGVLEVRPGEGTFVREVAAD
KSFHSLTLLLLLGTTLEVLEVRKILESGAVVLATERATDEDIKRLEDAVVKMEKDLKQGDLGDEADFMFHYGLALATHNS
LLVRLMNSIADTMRQSLKINRERLFRTPGMPEIFYKYHKEILEAIKARDKIRAQKVLEEHLTVAEEKLLEE

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=236, Percent_Identity=35.5932203389831, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790032, Length=221, Percent_Identity=33.0316742081448, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1790780, Length=239, Percent_Identity=32.6359832635983, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789353, Length=227, Percent_Identity=29.9559471365639, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1789621, Length=227, Percent_Identity=30.8370044052863, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082208, Length=219, Percent_Identity=27.8538812785388, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI48994955, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786950, Length=79, Percent_Identity=46.8354430379747, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26223; Mature: 26223

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQQIKTKKIYEEIIEQIRLLIARGELKPGDKLPSERELAESLGVSRASVREALSALEV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
LGVLEVRPGEGTFVREVAADKSFHSLTLLLLLGTTLEVLEVRKILESGAVVLATERATDE
HHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHH
DIKRLEDAVVKMEKDLKQGDLGDEADFMFHYGLALATHNSLLVRLMNSIADTMRQSLKIN
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERLFRTPGMPEIFYKYHKEILEAIKARDKIRAQKVLEEHLTVAEEKLLEE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFQQIKTKKIYEEIIEQIRLLIARGELKPGDKLPSERELAESLGVSRASVREALSALEV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
LGVLEVRPGEGTFVREVAADKSFHSLTLLLLLGTTLEVLEVRKILESGAVVLATERATDE
HHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHH
DIKRLEDAVVKMEKDLKQGDLGDEADFMFHYGLALATHNSLLVRLMNSIADTMRQSLKIN
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERLFRTPGMPEIFYKYHKEILEAIKARDKIRAQKVLEEHLTVAEEKLLEE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]