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The map label for this gene is leuB

Identifier: 78044738

GI number: 78044738

Start: 456170

End: 457294

Strand: Direct

Name: leuB

Synonym: CHY_0524

Alternate gene names: 78044738

Gene position: 456170-457294 (Clockwise)

Preceding gene: 78043773

Following gene: 78042646

Centisome position: 19.0

GC content: 45.42

Gene sequence:

>1125_bases
ATGTCCGGGAGAAGCTGCAAGGGACCGTTTAAAATTTTGGTGCTGCCGGGGGATGGTATTGGACCGGAGATAATTAAGGA
AGCGGTGAAGGTTTTAAAAGCGTTAGGGGAGAAAAAGGGCATTACTTTTAATTTTCAGTACGGTTTAATCGGGGGAGCGG
CCATCGACGAAAGGGGAGTGCCCCTTCCCGAAGAAACGGTGGAACTTGGAAAACAATGTGATGCTATTTTGTTAGGGGCT
GTAGGGGGGCCCAAGTGGGATAATTTGCCGCCGGAAATTCGGCCCGAATTGGGAGGGCTTTTAAAAATTCGCAAAGTATT
TGACCTGTATGCCAACCTGCGGCCGGTAATGTTTTTTCCGGAGCTGAAAAATGCTTCCCCTTTAAAGCCGGACATAATTG
AGGGTGTGGATATTTTAATGGTCCGGGAGTTAACGGGAGGTCTTTATTTTGGCGAGAAGAAGCGGTTCACTACCGAGCAA
GGGGAACAAGCGGTAATTGATACCTTGATTTATACCGAAAAAGAAGTGGAAAGGGTAGTAAGACTTGGTTTTGAGTTAGC
GCAAAAACGAAGAGGTAAACTTACTTTGGTGGATAAAGCCAATGTTTTAGAAAGCTCCCGTTTTTGGCGGGAGATAACCG
GGGAGATTAAAAAAGAGTATCCGGATGTTGAGCTTTCCTACATGTATGTTGATAACTGTGCAATGCAGCTTATAAGGAAT
CCCCGGCAGTTTGATGTAATCGTTACCGAAAACATGTTTGGGGATATTTTAACCGATGAAGGCTCGGTTTTGGCCGGTTC
CATTGGCCTTTTACCCTCGGCCAGCTTAAACGGAAAGTTTGGGTTATATGAGCCGATCCACGGTTCGGCACCGGATATTG
CCGGACAAAACAAAGCTAACCCCCTGGCGACAATTTTATCCGCCGGGATGATGTTAAGATACAGTTTAGACTGCCCGGAA
GAAGCGTTACTTATTGAAAAAGCGGTAAAAGCAGTATTACAAAAAGGTTACCGGACGGGTGATATTTTGGAGCCGGGTAC
TACGTTAGTTACCTGTGAAGAAATGGGTGATTTAGTAGCGGAAGAAATCTTAGGTGGGGTTTATGATGAAGGTTTACCTT
TATGA

Upstream 100 bases:

>100_bases
ACCGGAGAAATAAAAAATCTTACTACCGGCGAAAGCTACTTTGCCAAACCCATACCGGAGTTTATGCAGAATATTATTGC
CAAAGGAGGACTTATTAACT

Downstream 100 bases:

>100_bases
TACTACTTTAAGGGATGGAGCTCAGGCGGAAGGGATAAATTATACCGTTTCCGATAAATTAAAAATTGCCAGGAAATTAA
TTGCCTTTGGCATTGATTAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSGRSCKGPFKILVLPGDGIGPEIIKEAVKVLKALGEKKGITFNFQYGLIGGAAIDERGVPLPEETVELGKQCDAILLGA
VGGPKWDNLPPEIRPELGGLLKIRKVFDLYANLRPVMFFPELKNASPLKPDIIEGVDILMVRELTGGLYFGEKKRFTTEQ
GEQAVIDTLIYTEKEVERVVRLGFELAQKRRGKLTLVDKANVLESSRFWREITGEIKKEYPDVELSYMYVDNCAMQLIRN
PRQFDVIVTENMFGDILTDEGSVLAGSIGLLPSASLNGKFGLYEPIHGSAPDIAGQNKANPLATILSAGMMLRYSLDCPE
EALLIEKAVKAVLQKGYRTGDILEPGTTLVTCEEMGDLVAEEILGGVYDEGLPL

Sequences:

>Translated_374_residues
MSGRSCKGPFKILVLPGDGIGPEIIKEAVKVLKALGEKKGITFNFQYGLIGGAAIDERGVPLPEETVELGKQCDAILLGA
VGGPKWDNLPPEIRPELGGLLKIRKVFDLYANLRPVMFFPELKNASPLKPDIIEGVDILMVRELTGGLYFGEKKRFTTEQ
GEQAVIDTLIYTEKEVERVVRLGFELAQKRRGKLTLVDKANVLESSRFWREITGEIKKEYPDVELSYMYVDNCAMQLIRN
PRQFDVIVTENMFGDILTDEGSVLAGSIGLLPSASLNGKFGLYEPIHGSAPDIAGQNKANPLATILSAGMMLRYSLDCPE
EALLIEKAVKAVLQKGYRTGDILEPGTTLVTCEEMGDLVAEEILGGVYDEGLPL
>Mature_373_residues
SGRSCKGPFKILVLPGDGIGPEIIKEAVKVLKALGEKKGITFNFQYGLIGGAAIDERGVPLPEETVELGKQCDAILLGAV
GGPKWDNLPPEIRPELGGLLKIRKVFDLYANLRPVMFFPELKNASPLKPDIIEGVDILMVRELTGGLYFGEKKRFTTEQG
EQAVIDTLIYTEKEVERVVRLGFELAQKRRGKLTLVDKANVLESSRFWREITGEIKKEYPDVELSYMYVDNCAMQLIRNP
RQFDVIVTENMFGDILTDEGSVLAGSIGLLPSASLNGKFGLYEPIHGSAPDIAGQNKANPLATILSAGMMLRYSLDCPEE
ALLIEKAVKAVLQKGYRTGDILEPGTTLVTCEEMGDLVAEEILGGVYDEGLPL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=31.9402985074627, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI28178816, Length=317, Percent_Identity=29.6529968454259, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI28178821, Length=317, Percent_Identity=29.6529968454259, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=30.635838150289, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI28178838, Length=330, Percent_Identity=30.3030303030303, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI28178819, Length=164, Percent_Identity=35.9756097560976, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=54.016620498615, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788101, Length=348, Percent_Identity=37.3563218390805, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787381, Length=401, Percent_Identity=27.431421446384, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71986051, Length=356, Percent_Identity=32.3033707865169, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI25144293, Length=340, Percent_Identity=28.5294117647059, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=29.3948126801153, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17550882, Length=348, Percent_Identity=30.1724137931034, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=51.0869565217391, Blast_Score=336, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=36.4942528735632, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=354, Percent_Identity=32.2033898305085, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=349, Percent_Identity=32.9512893982808, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=31.8713450292398, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=31.8713450292398, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24661184, Length=350, Percent_Identity=30.5714285714286, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078635, Length=343, Percent_Identity=27.9883381924198, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078637, Length=343, Percent_Identity=27.9883381924198, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078633, Length=343, Percent_Identity=27.9883381924198, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24650122, Length=343, Percent_Identity=27.9883381924198, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=28.1899109792285, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281362242, Length=342, Percent_Identity=29.2397660818713, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648872, Length=342, Percent_Identity=29.2397660818713, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI20130355, Length=213, Percent_Identity=25.8215962441315, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_CARHZ (Q3AEQ2)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359382.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q3AEQ2
- SMR:   Q3AEQ2
- STRING:   Q3AEQ2
- GeneID:   3727861
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0524
- NMPDR:   fig|246194.3.peg.1091
- TIGR:   CHY_0524
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   CHYD246194:CHY_0524-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 40975; Mature: 40844

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 104-104 BINDING 114-114 BINDING 142-142 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRSCKGPFKILVLPGDGIGPEIIKEAVKVLKALGEKKGITFNFQYGLIGGAAIDERGV
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCC
PLPEETVELGKQCDAILLGAVGGPKWDNLPPEIRPELGGLLKIRKVFDLYANLRPVMFFP
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEC
ELKNASPLKPDIIEGVDILMVRELTGGLYFGEKKRFTTEQGEQAVIDTLIYTEKEVERVV
CCCCCCCCCCHHHCCCHHEEHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHH
RLGFELAQKRRGKLTLVDKANVLESSRFWREITGEIKKEYPDVELSYMYVDNCAMQLIRN
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
PRQFDVIVTENMFGDILTDEGSVLAGSIGLLPSASLNGKFGLYEPIHGSAPDIAGQNKAN
CCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLATILSAGMMLRYSLDCPEEALLIEKAVKAVLQKGYRTGDILEPGTTLVTCEEMGDLVA
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHH
EEILGGVYDEGLPL
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SGRSCKGPFKILVLPGDGIGPEIIKEAVKVLKALGEKKGITFNFQYGLIGGAAIDERGV
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCC
PLPEETVELGKQCDAILLGAVGGPKWDNLPPEIRPELGGLLKIRKVFDLYANLRPVMFFP
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEC
ELKNASPLKPDIIEGVDILMVRELTGGLYFGEKKRFTTEQGEQAVIDTLIYTEKEVERVV
CCCCCCCCCCHHHCCCHHEEHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHH
RLGFELAQKRRGKLTLVDKANVLESSRFWREITGEIKKEYPDVELSYMYVDNCAMQLIRN
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
PRQFDVIVTENMFGDILTDEGSVLAGSIGLLPSASLNGKFGLYEPIHGSAPDIAGQNKAN
CCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLATILSAGMMLRYSLDCPEEALLIEKAVKAVLQKGYRTGDILEPGTTLVTCEEMGDLVA
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHH
EEILGGVYDEGLPL
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA