Click here to switch to the map view.

The map label for this gene is cysL [H]

Identifier: 78044734

GI number: 78044734

Start: 10537

End: 11430

Strand: Direct

Name: cysL [H]

Synonym: CHY_0013

Alternate gene names: 78044734

Gene position: 10537-11430 (Clockwise)

Preceding gene: 78043356

Following gene: 78043587

Centisome position: 0.44

GC content: 47.87

Gene sequence:

>894_bases
TTGAACCGAAATCAGCTGGAAATTTTCCGGGTGGTGGCGGAAAAGAAAAGTTTTTCTGAAGCTGCTCGCTTACTGCATAT
TTCTCAGCCTGCCATTAGTATGCATATCCAGGCTTTAGAAGAATATTATGGGACAAAGCTTCTTGACAGGACCACTAAGC
GGGTTACCCTCACCCCGGCAGGAGAAATTCTCTATAAGTACACCCTGCACCTGTTATCCCTAATGGACAAAGCTCAACGG
GATATTAGCCAGGCTGCTGGTATCATTAAGGGCAAGCTTATTTTGGGGGCGAGTTTAACCATCGGGGGTCATGTTTTACC
CCCGCTGCTTAGCTCCTTTTACCGAAAATACCCGGATGTAAATATTACCATGGAAATAATGAATACCGAAAAGGTGCTGG
AACGGGTAAGCGATCTTACGTTAGATCTGGGTTTTGTAGAGGGAGAGGTAACCACCGGAGAACTTCACGTGGAAGCTTTA
GCCCGGGATGAACTGGTAGTAGTGGTAGCGGCCAACCATCCTCTTGCTGGACGCGGGCGGATTTCCTGGGAGGAATTAGC
AACATTACCTCTGGTAATGCGGGAACCCGGTTCCGGTACCCGGAAGGTGATAGAAGAGCGACTGCAGGCAGCTGGCTTTA
AACTGCCGGAACTAAAAATAGTAATGGAACTGGGCAGCACCGAGGCAATAAAGGGAGCAGTCGAAGCGGGATTAGGAGCG
GCTATCCTTTCAAAGTGGACGGTAAGGCGGGAGATTAACTGGGGGATATTTAAGCAGCTTCTGATAGAAAATTTACCGAT
TTACCGGGAATTTTATGTAGTTTTAAATAATAATAAGTTTCACTCGCCGGCGACCCAGGCCCTGATTCAATTCTGCCGGG
AGCATCTATCGTAG

Upstream 100 bases:

>100_bases
GTGATCGGATCTCTGGCATTAGCCCTGTTTGGCTGGGGATTGCTGATATTATTTTCAATTACTTAAGCCTTAAAAAATAA
TGCAAAAAGGAGGTTCCTTT

Downstream 100 bases:

>100_bases
GTTTAAAGCTACCAAAAAGGAGCCGGAATACGGCTCCTTTTTAGCAATTAACTTTTAAACTGCTTCTGCCAAACGGCCAG
AGCTTCCTCATAAACTTCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNRNQLEIFRVVAEKKSFSEAARLLHISQPAISMHIQALEEYYGTKLLDRTTKRVTLTPAGEILYKYTLHLLSLMDKAQR
DISQAAGIIKGKLILGASLTIGGHVLPPLLSSFYRKYPDVNITMEIMNTEKVLERVSDLTLDLGFVEGEVTTGELHVEAL
ARDELVVVVAANHPLAGRGRISWEELATLPLVMREPGSGTRKVIEERLQAAGFKLPELKIVMELGSTEAIKGAVEAGLGA
AILSKWTVRREINWGIFKQLLIENLPIYREFYVVLNNNKFHSPATQALIQFCREHLS

Sequences:

>Translated_297_residues
MNRNQLEIFRVVAEKKSFSEAARLLHISQPAISMHIQALEEYYGTKLLDRTTKRVTLTPAGEILYKYTLHLLSLMDKAQR
DISQAAGIIKGKLILGASLTIGGHVLPPLLSSFYRKYPDVNITMEIMNTEKVLERVSDLTLDLGFVEGEVTTGELHVEAL
ARDELVVVVAANHPLAGRGRISWEELATLPLVMREPGSGTRKVIEERLQAAGFKLPELKIVMELGSTEAIKGAVEAGLGA
AILSKWTVRREINWGIFKQLLIENLPIYREFYVVLNNNKFHSPATQALIQFCREHLS
>Mature_297_residues
MNRNQLEIFRVVAEKKSFSEAARLLHISQPAISMHIQALEEYYGTKLLDRTTKRVTLTPAGEILYKYTLHLLSLMDKAQR
DISQAAGIIKGKLILGASLTIGGHVLPPLLSSFYRKYPDVNITMEIMNTEKVLERVSDLTLDLGFVEGEVTTGELHVEAL
ARDELVVVVAANHPLAGRGRISWEELATLPLVMREPGSGTRKVIEERLQAAGFKLPELKIVMELGSTEAIKGAVEAGLGA
AILSKWTVRREINWGIFKQLLIENLPIYREFYVVLNNNKFHSPATQALIQFCREHLS

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=288, Percent_Identity=32.9861111111111, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1788748, Length=294, Percent_Identity=29.2517006802721, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=31.7391304347826, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693105, Length=240, Percent_Identity=30, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787879, Length=256, Percent_Identity=32.421875, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=26.530612244898, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787530, Length=260, Percent_Identity=28.4615384615385, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=28.3400809716599, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787601, Length=294, Percent_Identity=27.2108843537415, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=27.843137254902, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788887, Length=199, Percent_Identity=31.1557788944724, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790262, Length=297, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081904, Length=253, Percent_Identity=24.901185770751, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693193, Length=269, Percent_Identity=30.4832713754647, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789204, Length=297, Percent_Identity=25.5892255892256, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=169, Percent_Identity=27.2189349112426, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082132, Length=304, Percent_Identity=23.6842105263158, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=174, Percent_Identity=28.1609195402299, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI2367136, Length=289, Percent_Identity=25.9515570934256, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787949, Length=291, Percent_Identity=25.4295532646048, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789173, Length=167, Percent_Identity=28.1437125748503, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=27.7591973244147, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=23.4817813765182, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788706, Length=167, Percent_Identity=28.1437125748503, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786713, Length=243, Percent_Identity=27.5720164609054, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33211; Mature: 33211

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNQLEIFRVVAEKKSFSEAARLLHISQPAISMHIQALEEYYGTKLLDRTTKRVTLTPA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHCCCCEEEECCH
GEILYKYTLHLLSLMDKAQRDISQAAGIIKGKLILGASLTIGGHVLPPLLSSFYRKYPDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHCCCC
NITMEIMNTEKVLERVSDLTLDLGFVEGEVTTGELHVEALARDELVVVVAANHPLAGRGR
EEEEEECCHHHHHHHHHHCEEEEEEEECEEEECCEEEEEECCCCEEEEEECCCCCCCCCC
ISWEELATLPLVMREPGSGTRKVIEERLQAAGFKLPELKIVMELGSTEAIKGAVEAGLGA
CCHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCHHH
AILSKWTVRREINWGIFKQLLIENLPIYREFYVVLNNNKFHSPATQALIQFCREHLS
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRNQLEIFRVVAEKKSFSEAARLLHISQPAISMHIQALEEYYGTKLLDRTTKRVTLTPA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHCCCCEEEECCH
GEILYKYTLHLLSLMDKAQRDISQAAGIIKGKLILGASLTIGGHVLPPLLSSFYRKYPDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHCCCC
NITMEIMNTEKVLERVSDLTLDLGFVEGEVTTGELHVEALARDELVVVVAANHPLAGRGR
EEEEEECCHHHHHHHHHHCEEEEEEEECEEEECCEEEEEECCCCEEEEEECCCCCCCCCC
ISWEELATLPLVMREPGSGTRKVIEERLQAAGFKLPELKIVMELGSTEAIKGAVEAGLGA
CCHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCHHH
AILSKWTVRREINWGIFKQLLIENLPIYREFYVVLNNNKFHSPATQALIQFCREHLS
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]