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The map label for this gene is lipA [C]

Identifier: 78044603

GI number: 78044603

Start: 429043

End: 429993

Strand: Direct

Name: lipA [C]

Synonym: CHY_0494

Alternate gene names: 78044603

Gene position: 429043-429993 (Clockwise)

Preceding gene: 78044055

Following gene: 78042941

Centisome position: 17.87

GC content: 44.79

Gene sequence:

>951_bases
ATGAGGGCATCGATTGGGACCCTGGCGGCGTTAAAGCTTAAAAAAGTCAAGGTAGAGGCTCTTCCCACTACTGCATATTT
TATGCTCGGTGAAAAGTGCCAGCATGATTGTGCCTTTTGTTCCCAGGCGCGCTCCAGTCACGCCACTCCTGAGCTTTTAT
CCCGGGTAACCTGGCCAAAAATAGAGGAGGAGGCGGTTTTTAAAGCGCTGGGGGAGGAACCTTCTGAAATACGCCGGGCT
TGTTTTCAAGTCGTGCGCACTAAAGAAAGTTTTGCGGAAATTTTGGAGAAGGCCAAAAAGTTAAAAGAAAAAGCGGCAAT
ACCTCTTTGTGTTTCGTACAATGTTCGGGATGCCATTGAGGCTCAACAGATATTTGCGGCCGGGGCTGACCATATAAGTC
TTCCTTTAGACGGTGCGGTTAAAAGGGTTTATGAAAAAGTTAAAGGCAAAAATTATGAGAAGTTTTACAATAAGCTTCTG
GACTTAGCCGAGAAGTTTCCGGGGAAAATAGGTACCCATTTAATTGCGGGCCTTGGGGAGACCGAAGAGGAAATGCTGAA
AGTAATGGACGATCTTCTAAAACACCAGATTACCATAGCTTTATTTGCTTTCACTCCCGTACCCGGGACCCGGTTAGAAA
AAGAAAGTCCACCGGATATAAAAAGTTACCGCCGGATTCAAGCGGGTTTTTATCTTTTAAAAAAGAATTTAAAAAGTATT
TCTGAATTTACCTTTAAAAATGGACGGGTAGTAAGCTTTGGCTTGACGCGGGAGGAAATCCTGGAAATTTTTCAGGATGG
CGAAGCTTTTCGCACCAGCGGTTGTCCTGACTGTAACCGTCCTTATTACAACGAACGGCCGGGAGGAACAATATATAATT
ATCCTCGCCCGTTGACTCCTGAAGAAAGAGAGCGGTGTTTGGAAGAACTTTTAACCGAACTGGAGGTGTAA

Upstream 100 bases:

>100_bases
AGCTGGGCTTTGCGCGGATTGTGCAGCCCCATCCGGAGGTAGTGAAAAAAGCCCGGGAATTGGGATATGAAATTGAGGAA
GCGGGAGAGTGCTGCGTGTT

Downstream 100 bases:

>100_bases
TATGGAAACCTGGCGCTTTATCGATCACTGCTCCTTTTCCGGAGCAGAAAATATGGCGATTGATGAAGCATTATTACAGG
AAGCTATAAATAATCACGGG

Product: radical SAM domain-containing protein

Products: biotin

Alternate protein names: Radical SAM Domain-Containing Protein; Biotin Synthase Related Protein; Biotin Synthase; Biotin Synthase-Related Protein; Biotin Synthase-Related; Radical SAM Superfamily Protein; Radical SAM Family Protein

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRASIGTLAALKLKKVKVEALPTTAYFMLGEKCQHDCAFCSQARSSHATPELLSRVTWPKIEEEAVFKALGEEPSEIRRA
CFQVVRTKESFAEILEKAKKLKEKAAIPLCVSYNVRDAIEAQQIFAAGADHISLPLDGAVKRVYEKVKGKNYEKFYNKLL
DLAEKFPGKIGTHLIAGLGETEEEMLKVMDDLLKHQITIALFAFTPVPGTRLEKESPPDIKSYRRIQAGFYLLKKNLKSI
SEFTFKNGRVVSFGLTREEILEIFQDGEAFRTSGCPDCNRPYYNERPGGTIYNYPRPLTPEERERCLEELLTELEV

Sequences:

>Translated_316_residues
MRASIGTLAALKLKKVKVEALPTTAYFMLGEKCQHDCAFCSQARSSHATPELLSRVTWPKIEEEAVFKALGEEPSEIRRA
CFQVVRTKESFAEILEKAKKLKEKAAIPLCVSYNVRDAIEAQQIFAAGADHISLPLDGAVKRVYEKVKGKNYEKFYNKLL
DLAEKFPGKIGTHLIAGLGETEEEMLKVMDDLLKHQITIALFAFTPVPGTRLEKESPPDIKSYRRIQAGFYLLKKNLKSI
SEFTFKNGRVVSFGLTREEILEIFQDGEAFRTSGCPDCNRPYYNERPGGTIYNYPRPLTPEERERCLEELLTELEV
>Mature_316_residues
MRASIGTLAALKLKKVKVEALPTTAYFMLGEKCQHDCAFCSQARSSHATPELLSRVTWPKIEEEAVFKALGEEPSEIRRA
CFQVVRTKESFAEILEKAKKLKEKAAIPLCVSYNVRDAIEAQQIFAAGADHISLPLDGAVKRVYEKVKGKNYEKFYNKLL
DLAEKFPGKIGTHLIAGLGETEEEMLKVMDDLLKHQITIALFAFTPVPGTRLEKESPPDIKSYRRIQAGFYLLKKNLKSI
SEFTFKNGRVVSFGLTREEILEIFQDGEAFRTSGCPDCNRPYYNERPGGTIYNYPRPLTPEERERCLEELLTELEV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group. Free octanoate is not a substrate for lipA. But is also able to catalyze sulfur insertion into an octanoy

COG id: COG2516

COG function: function code R; Biotin synthase-related enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.6

Molecular weight: Translated: 35791; Mature: 35791

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASIGTLAALKLKKVKVEALPTTAYFMLGEKCQHDCAFCSQARSSHATPELLSRVTWPK
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
IEEEAVFKALGEEPSEIRRACFQVVRTKESFAEILEKAKKLKEKAAIPLCVSYNVRDAIE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
AQQIFAAGADHISLPLDGAVKRVYEKVKGKNYEKFYNKLLDLAEKFPGKIGTHLIAGLGE
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
TEEEMLKVMDDLLKHQITIALFAFTPVPGTRLEKESPPDIKSYRRIQAGFYLLKKNLKSI
CHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SEFTFKNGRVVSFGLTREEILEIFQDGEAFRTSGCPDCNRPYYNERPGGTIYNYPRPLTP
HHHHHCCCEEEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EERERCLEELLTELEV
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRASIGTLAALKLKKVKVEALPTTAYFMLGEKCQHDCAFCSQARSSHATPELLSRVTWPK
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
IEEEAVFKALGEEPSEIRRACFQVVRTKESFAEILEKAKKLKEKAAIPLCVSYNVRDAIE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
AQQIFAAGADHISLPLDGAVKRVYEKVKGKNYEKFYNKLLDLAEKFPGKIGTHLIAGLGE
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
TEEEMLKVMDDLLKHQITIALFAFTPVPGTRLEKESPPDIKSYRRIQAGFYLLKKNLKSI
CHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SEFTFKNGRVVSFGLTREEILEIFQDGEAFRTSGCPDCNRPYYNERPGGTIYNYPRPLTP
HHHHHCCCEEEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EERERCLEELLTELEV
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA