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The map label for this gene is cobS

Identifier: 78044598

GI number: 78044598

Start: 686678

End: 687445

Strand: Direct

Name: cobS

Synonym: CHY_0779

Alternate gene names: 78044598

Gene position: 686678-687445 (Clockwise)

Preceding gene: 78045040

Following gene: 78042928

Centisome position: 28.59

GC content: 44.01

Gene sequence:

>768_bases
ATGTTAACTACCTTTCTTTTAGGCCTTACCTTTTTCACCAGGATTCCGGTGCCGGGAAAACTTAATTTTTCCGAAGAAAA
ATTTAACAGGGCTCCTATCTTTCTTCCCGCTTATGGTTTAGTTACCGGTGGTATCTTAGCCTTAATAATTGAATTATTCG
GAAGGTCCTTTCCAGGCTTTTTCTGGGCCGGAGTTATAATAGCCGGGCAAATCTATTTATCGGGAGCCCTGCATATCGAT
GGGTTACTGGATAGCCTTGATGCAATTTACTCCAACCGCGACCGGGAAAAAAGGCTGGAAATTTTAAAGGACTCCCGGGT
AGGCTCGATGGCGGTGGCGTTTTTTGGAGCGTTTTTAATTCTAAAATACGGAAGTTATGCTTCCTTTACTCCGAAAGTGC
AGGCCTTTACGGTGCTGATTTCCGAAATTATCCTGCGTGGAACGGGCTACCTGGTGATTTATTCCTTCCCCTATGTGGGC
TCTTCCCTGGGACGGGGTTTTAAAGATAATGCTTCAACTGCCGGGTTAATCTTTACCCTGGGGCAAACCTTAATCTTTAC
CCTGGGAGCAGCGGCGTTTTTTAATTTTTCTCTAATTAAAATTTTAATAATTTTGCTTTTGGCTTATCTTTTTGCGTTTG
TGGTGGCAGCTCGCTGGCAGCAGTTTTTTGGGGGACTAACGGGAGATAATTACGGCGGAATTATGGAATTAACCGGCCTT
TTTGTGCCGGTGGCGGTTTTACTTATCAATAATATTGGGGTGGTGTGA

Upstream 100 bases:

>100_bases
GGAATCAAGCATCCGGCGGTAATTGTGGTGGGTGATGTGGTAAAATTACAAGGGGAGCTTGGCTGGTATTCCCCATCAGC
ATTAACGGAGTGAGCTTAAA

Downstream 100 bases:

>100_bases
TACCACCCCTTTTAGCTTTTAATGGATTGTTAAGGTAAAATTTGCTTTTATTTCTCCGACCATTTCTTTTTTGTGATTTT
TAATAACCGGACGGTAGTGG

Product: putative cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTTFLLGLTFFTRIPVPGKLNFSEEKFNRAPIFLPAYGLVTGGILALIIELFGRSFPGFFWAGVIIAGQIYLSGALHID
GLLDSLDAIYSNRDREKRLEILKDSRVGSMAVAFFGAFLILKYGSYASFTPKVQAFTVLISEIILRGTGYLVIYSFPYVG
SSLGRGFKDNASTAGLIFTLGQTLIFTLGAAAFFNFSLIKILIILLLAYLFAFVVAARWQQFFGGLTGDNYGGIMELTGL
FVPVAVLLINNIGVV

Sequences:

>Translated_255_residues
MLTTFLLGLTFFTRIPVPGKLNFSEEKFNRAPIFLPAYGLVTGGILALIIELFGRSFPGFFWAGVIIAGQIYLSGALHID
GLLDSLDAIYSNRDREKRLEILKDSRVGSMAVAFFGAFLILKYGSYASFTPKVQAFTVLISEIILRGTGYLVIYSFPYVG
SSLGRGFKDNASTAGLIFTLGQTLIFTLGAAAFFNFSLIKILIILLLAYLFAFVVAARWQQFFGGLTGDNYGGIMELTGL
FVPVAVLLINNIGVV
>Mature_255_residues
MLTTFLLGLTFFTRIPVPGKLNFSEEKFNRAPIFLPAYGLVTGGILALIIELFGRSFPGFFWAGVIIAGQIYLSGALHID
GLLDSLDAIYSNRDREKRLEILKDSRVGSMAVAFFGAFLILKYGSYASFTPKVQAFTVLISEIILRGTGYLVIYSFPYVG
SSLGRGFKDNASTAGLIFTLGQTLIFTLGAAAFFNFSLIKILIILLLAYLFAFVVAARWQQFFGGLTGDNYGGIMELTGL
FVPVAVLLINNIGVV

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=259, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_CARHZ (Q3AE01)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359633.1
- STRING:   Q3AE01
- GeneID:   3727719
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0779
- NMPDR:   fig|246194.3.peg.929
- TIGR:   CHY_0779
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   ELTECAV
- BioCyc:   CHYD246194:CHY_0779-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27809; Mature: 27809

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x104a76ac)-; HASH(0x1180ba20)-; HASH(0x1171a26c)-; HASH(0x11857f68)-; HASH(0x11411b5c)-; HASH(0x953c3a4)-; HASH(0x11819660)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCH
>Mature Secondary Structure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HHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA