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The map label for this gene is ilvB1 [H]

Identifier: 78044561

GI number: 78044561

Start: 447992

End: 449650

Strand: Direct

Name: ilvB1 [H]

Synonym: CHY_0517

Alternate gene names: 78044561

Gene position: 447992-449650 (Clockwise)

Preceding gene: 78045160

Following gene: 78043291

Centisome position: 18.65

GC content: 48.04

Gene sequence:

>1659_bases
ATGGAGTTAACCGGAGCGCAAATTTTAATTAAAGTATTAGAGGATTTAGGAGTGGATACTGTATTTGGCTATCCCGGTGG
AGCGGTACTGCCCATTTACGATGCCCTTTATGATTCAAAAATTACCCATTACTTGACCCGGCACGAACAGGGAGCGGCCC
ATGCTGCCGATGGCTATGCCCGCGCTTCGGGGAAGGTAGGGGTATGTTTTGCAACTTCCGGCCCAGGAGCCACTAACCTG
GTAACCGGTCTTGCCACTGCCTATATGGATTCGGTTCCGGTGGTTGCCATTACCGGACAGGTGGCAACCTCGCTTTTGGG
ACGGGATTCCTTTCAGGAAGCGGATATTACCGGCATAACCATGCCGATTACCAAACATAATTATTTGGTAAAAGATCCGG
GGGAACTGGCCCAGACGGTGGTGGAAGCATTTTACATTGCCCGGACGGGACGACCGGGGCCGGTTTTAATTGATATTCCC
AAGGATGTTTCGGCGGCCCGGGCGCGCTATGAATTTCCCGAAAAAGTTGAGCTTCCAGGGTACAAACCGGTTTTAACTCC
CGGCGAGGAAGAGGTTAATAAGGCTATAGCGTTAATTAATTCCTCCGAGCGTCCGTTAATTTTTAGCGGAGGCGGGACTG
TTAACGCCGGGGCCGAGCAATTATTGCTGGCTTTTGCCGAGAAAATCAATGCACCGGTGGTTGCAAGTTTAATGGGTTTA
GGAGGATTTCCCGGAGACCATCCCTTATTCTTGGGAATGCTGGGTATGCACGGGACCAAGCCGGCCAATTATGCGGTCAG
CGAATGTGATGTCCTGATAGCCGTGGGAGTGCGCTTTGACGACCGGGTAACCGGAGATGTTAAAAGCTTTGCCCCTAATG
CCAAGATTGTTCACATAGATATTGACCCGGCGGAAATCAATAAGAATGTGCTGGTGGAAGTGCCTTTGGTGGGGGATGTA
AAGAACAGCTTAGAGCTTTTAATTAAAGGGGTAAAGGAAAAAAAGCCGGAGAAATGGCTTAAGTATGTTTTTGGTTTAAA
GGAAAAATATCCTTTAACTTATAACCGGGAGTGTGGCTTAAAACCCCAAAAAGTTATCGAAACTCTTTATAAGATAACCG
GAGGCGAAGCAATTATCGTTACCGATGTGGGACAGCATCAGATGTGGGCTGCCCAGTTTTACAAATTTAATAAGCCGAGG
AGATTTATTTCCTCCGGTGGGTTAGGAACGATGGGGTTTGGTTTACCGGCAGGAATTGGGGCTCAGGTGGCAAGACCGGA
GCAGCAGGTAATAGTGGTATCGGGCGATGGTAGTATCCAGATGAATAGCCAGGAACTAATGACCGTTGCGGCCTATAATA
TCCCCGTTAAGGTTATTATCTTAAACAACGGTTACCTGGGGATGGTGCGGCAGTGGCAGGAACTTTTCCATCAGCGCCGT
TATTCGCATACGGAACTTAAAAATCCCGACTTTGTTAAGTTAGCCGATGCTTACGGGATAAGGGGTTTGCGCATTAAAAA
AGAAGAAAACTTGGAAGAAAAACTTTTGGAGGCAATAAATTATGCCGGACCGGTAGTGGTAGATGTTTGGGTAGACCGGG
AGGAAAATGTTTTTCCCATGGTGCCACCGGGAGGCGCAATTCACAAAATGATCGGTTAA

Upstream 100 bases:

>100_bases
TTCCCGAGCCTAAGATTAAAGAAGGTTACCTTGCTCGTTATCAAAAATTAGTGAGCTCTGCTAATTTAGGTGCGGTGTTT
TTAAAGTAGGGGTGAGCGAA

Downstream 100 bases:

>100_bases
AGGGGGGATTTGATGCGGCATACCCTGGCAGTATTGGTGGAAAACAATCCTGGGGTTTTAGCAAGGGTGGCGGGGCTTTT
TAGCCGCCGGGGCTATAACA

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MELTGAQILIKVLEDLGVDTVFGYPGGAVLPIYDALYDSKITHYLTRHEQGAAHAADGYARASGKVGVCFATSGPGATNL
VTGLATAYMDSVPVVAITGQVATSLLGRDSFQEADITGITMPITKHNYLVKDPGELAQTVVEAFYIARTGRPGPVLIDIP
KDVSAARARYEFPEKVELPGYKPVLTPGEEEVNKAIALINSSERPLIFSGGGTVNAGAEQLLLAFAEKINAPVVASLMGL
GGFPGDHPLFLGMLGMHGTKPANYAVSECDVLIAVGVRFDDRVTGDVKSFAPNAKIVHIDIDPAEINKNVLVEVPLVGDV
KNSLELLIKGVKEKKPEKWLKYVFGLKEKYPLTYNRECGLKPQKVIETLYKITGGEAIIVTDVGQHQMWAAQFYKFNKPR
RFISSGGLGTMGFGLPAGIGAQVARPEQQVIVVSGDGSIQMNSQELMTVAAYNIPVKVIILNNGYLGMVRQWQELFHQRR
YSHTELKNPDFVKLADAYGIRGLRIKKEENLEEKLLEAINYAGPVVVDVWVDREENVFPMVPPGGAIHKMIG

Sequences:

>Translated_552_residues
MELTGAQILIKVLEDLGVDTVFGYPGGAVLPIYDALYDSKITHYLTRHEQGAAHAADGYARASGKVGVCFATSGPGATNL
VTGLATAYMDSVPVVAITGQVATSLLGRDSFQEADITGITMPITKHNYLVKDPGELAQTVVEAFYIARTGRPGPVLIDIP
KDVSAARARYEFPEKVELPGYKPVLTPGEEEVNKAIALINSSERPLIFSGGGTVNAGAEQLLLAFAEKINAPVVASLMGL
GGFPGDHPLFLGMLGMHGTKPANYAVSECDVLIAVGVRFDDRVTGDVKSFAPNAKIVHIDIDPAEINKNVLVEVPLVGDV
KNSLELLIKGVKEKKPEKWLKYVFGLKEKYPLTYNRECGLKPQKVIETLYKITGGEAIIVTDVGQHQMWAAQFYKFNKPR
RFISSGGLGTMGFGLPAGIGAQVARPEQQVIVVSGDGSIQMNSQELMTVAAYNIPVKVIILNNGYLGMVRQWQELFHQRR
YSHTELKNPDFVKLADAYGIRGLRIKKEENLEEKLLEAINYAGPVVVDVWVDREENVFPMVPPGGAIHKMIG
>Mature_552_residues
MELTGAQILIKVLEDLGVDTVFGYPGGAVLPIYDALYDSKITHYLTRHEQGAAHAADGYARASGKVGVCFATSGPGATNL
VTGLATAYMDSVPVVAITGQVATSLLGRDSFQEADITGITMPITKHNYLVKDPGELAQTVVEAFYIARTGRPGPVLIDIP
KDVSAARARYEFPEKVELPGYKPVLTPGEEEVNKAIALINSSERPLIFSGGGTVNAGAEQLLLAFAEKINAPVVASLMGL
GGFPGDHPLFLGMLGMHGTKPANYAVSECDVLIAVGVRFDDRVTGDVKSFAPNAKIVHIDIDPAEINKNVLVEVPLVGDV
KNSLELLIKGVKEKKPEKWLKYVFGLKEKYPLTYNRECGLKPQKVIETLYKITGGEAIIVTDVGQHQMWAAQFYKFNKPR
RFISSGGLGTMGFGLPAGIGAQVARPEQQVIVVSGDGSIQMNSQELMTVAAYNIPVKVIILNNGYLGMVRQWQELFHQRR
YSHTELKNPDFVKLADAYGIRGLRIKKEENLEEKLLEAINYAGPVVVDVWVDREENVFPMVPPGGAIHKMIG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=592, Percent_Identity=24.3243243243243, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=24.5115452930728, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=48.8454706927176, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=45.8483754512635, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1786717, Length=552, Percent_Identity=35.6884057971014, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=33.1511839708561, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=26.3913824057451, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17531299, Length=491, Percent_Identity=29.5315682281059, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=491, Percent_Identity=29.5315682281059, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=534, Percent_Identity=25.0936329588015, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=47.5609756097561, Blast_Score=509, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6320588, Length=564, Percent_Identity=23.4042553191489, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=524, Percent_Identity=24.236641221374, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320816, Length=486, Percent_Identity=23.0452674897119, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=532, Percent_Identity=23.8721804511278, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323073, Length=526, Percent_Identity=23.574144486692, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320123, Length=534, Percent_Identity=22.8464419475655, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922626, Length=478, Percent_Identity=25.7322175732218, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59842; Mature: 59842

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTGAQILIKVLEDLGVDTVFGYPGGAVLPIYDALYDSKITHYLTRHEQGAAHAADGYA
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
RASGKVGVCFATSGPGATNLVTGLATAYMDSVPVVAITGQVATSLLGRDSFQEADITGIT
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCEEE
MPITKHNYLVKDPGELAQTVVEAFYIARTGRPGPVLIDIPKDVSAARARYEFPEKVELPG
EEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCC
YKPVLTPGEEEVNKAIALINSSERPLIFSGGGTVNAGAEQLLLAFAEKINAPVVASLMGL
CCCCCCCCHHHHHHHEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHC
GGFPGDHPLFLGMLGMHGTKPANYAVSECDVLIAVGVRFDDRVTGDVKSFAPNAKIVHID
CCCCCCCCHHHEECCCCCCCCCCCCCCCCCEEEEECCEECCCCCCCHHHCCCCCEEEEEE
IDPAEINKNVLVEVPLVGDVKNSLELLIKGVKEKKPEKWLKYVFGLKEKYPLTYNRECGL
CCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCC
KPQKVIETLYKITGGEAIIVTDVGQHQMWAAQFYKFNKPRRFISSGGLGTMGFGLPAGIG
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
AQVARPEQQVIVVSGDGSIQMNSQELMTVAAYNIPVKVIILNNGYLGMVRQWQELFHQRR
CCCCCCCCEEEEEECCCCEEECHHHEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHC
YSHTELKNPDFVKLADAYGIRGLRIKKEENLEEKLLEAINYAGPVVVDVWVDREENVFPM
CCCCCCCCCCEEEEEHHCCCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
VPPGGAIHKMIG
CCCCCHHHHHCC
>Mature Secondary Structure
MELTGAQILIKVLEDLGVDTVFGYPGGAVLPIYDALYDSKITHYLTRHEQGAAHAADGYA
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
RASGKVGVCFATSGPGATNLVTGLATAYMDSVPVVAITGQVATSLLGRDSFQEADITGIT
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCEEE
MPITKHNYLVKDPGELAQTVVEAFYIARTGRPGPVLIDIPKDVSAARARYEFPEKVELPG
EEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCC
YKPVLTPGEEEVNKAIALINSSERPLIFSGGGTVNAGAEQLLLAFAEKINAPVVASLMGL
CCCCCCCCHHHHHHHEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHC
GGFPGDHPLFLGMLGMHGTKPANYAVSECDVLIAVGVRFDDRVTGDVKSFAPNAKIVHID
CCCCCCCCHHHEECCCCCCCCCCCCCCCCCEEEEECCEECCCCCCCHHHCCCCCEEEEEE
IDPAEINKNVLVEVPLVGDVKNSLELLIKGVKEKKPEKWLKYVFGLKEKYPLTYNRECGL
CCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCC
KPQKVIETLYKITGGEAIIVTDVGQHQMWAAQFYKFNKPRRFISSGGLGTMGFGLPAGIG
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
AQVARPEQQVIVVSGDGSIQMNSQELMTVAAYNIPVKVIILNNGYLGMVRQWQELFHQRR
CCCCCCCCEEEEEECCCCEEECHHHEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHC
YSHTELKNPDFVKLADAYGIRGLRIKKEENLEEKLLEAINYAGPVVVDVWVDREENVFPM
CCCCCCCCCCEEEEEHHCCCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
VPPGGAIHKMIG
CCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]