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The map label for this gene is hydB [H]

Identifier: 78044500

GI number: 78044500

Start: 644371

End: 646149

Strand: Direct

Name: hydB [H]

Synonym: CHY_0732

Alternate gene names: 78044500

Gene position: 644371-646149 (Clockwise)

Preceding gene: 78044572

Following gene: 78044647

Centisome position: 26.83

GC content: 47.05

Gene sequence:

>1779_bases
ATGAGAAAGGTTTTGATTTGTGCGGGGACTGGTTGCATTTCGTCCGGTTCACCTAAAGTTACCGAAAAGTTTCGGGAAGA
ACTATCAAAAAGAGGTATTACCGATATAGAGGTTTACAATACCGGCTGTCACGGTTTTTGCGAACAGGGACCAATTGTGA
TCATTGAGCCCGATAAAACTTTTTACTGCCGGGTAGAGGTGGACGATGTACCGGAAATTATTGAAAAGCACCTTTTAGGT
GGACAAATTGTGGAAAAAGTTTTATTCCACGAACCCCAGGTAGACAAAAAGGTGGAAAAGTACACCGATATCTCTTTTTA
TGCTAAACAAAAGCGGCTGGTCCTCCATAACTGCGGGCATATTAACCCGGAACGGATTGAAGATTATGTAGCAAATGGTG
GTTATGAAGCCTTAAGAAAGGTACTTACTTCCATGTCGCCGCAGGAAGTGGTTGATGAAATTAAAAAATCCGGCTTACGC
GGACGGGGTGGCGGCGGTTTTCCTACCGGTTTGAAGTGGCAGTTTACCAGGGATGCCAAAGGGGATAAAAAATATGTGGT
GTGTAACGCCGACGAAGGCGACCCGGGAGCTTTTATGGACCGGTCCGTGTTAGAAGGAGATCCCCACGCGGTTTTAGAAG
GGATGGCCATTTGCGGCTATGCGGTGGGGGCTGACGAAGGTTATATCTACGTGCGGGCTGAGTACCCTCTGGCCATAAAA
CGCTTGCAAATTGCCATAAAACAGGCGGAAGAAAAGGGTTATTTAGGAGAAAATATCTTTGGTAGCGGCTTTAATTTCCG
GATAAAAATTAAAGCTGGTGCGGGAGCTTTTGTTTGCGGTGAAGAAACCGCCCTTTTGACTTCCATTGAAGGAAATAGAG
GAATGCCAAGACCGCGGCCGCCTTTTCCGGCGGTAAAAGGCCTCTGGGGTAAGCCCACCAATATCAATAACGTCGAAACA
TTTGCCAACGTGCCCTTAATCATCAAAAACGGCGCCGACTGGTATGCCTCCATGGGTACCGAAGGAAGTAAAGGAACAAA
GATTTTTGCTTTAACCGGTAAGGTTAATAATACCGGTCTGGTGGAAGTACCGATGGGCATTACCATGCGCCAGATAATTT
TTGATATTGGCGGTGGCATTAAGGGAGGTCGGAAGTTTAAAGCGGTACAAATTGGGGGTCCATCAGGTGGGTGCTTGCCG
GAAGAACTTTTAGACTTACCGGTAGATTATGATTCGTTAATTAAAGCCGGGGCGATGATGGGTTCCGGCGGGCTTGTGGT
AATGGACGAAACTACCTGCATGGTGGACGTGGCCAAGTTTTTCTTAAGATTTACCCAAAAGGAATCGTGCGGGAAATGCG
TTCCCTGCCGGGAAGGAACTAAACGGATGTTAGAAATCTTAGAAAAAATCACCGAAGGGAAAGGCGAACCCGGGGATCTT
GAAACCTTAGAAAAACTTGCCTATAACATTAAGAACTCCAGCCTTTGCGGTCTGGGCCAAACGGCACCCAATCCGGTTTT
ATCAACATTGAGGTATTTCCGGCATGAGTACGAGGCTCATATTTACGATAAAAAGTGTCCGGCCGGCGTTTGCGCGGCGC
TCTTAGAATATAAGATCCTTGAAGATAAATGTGTCGGTTGCGGCGCCTGTGCCCGGGTTTGCCCCGTGGGAGCAATCTCC
GGCGAAAGAAAACAGCCGCACCAGATTGACCCTGAAAAGTGCATTAAGTGCGGTTCGTGTATGGAAAAATGTAAGTTTGG
CGCAATAGTTAAAGGTTAA

Upstream 100 bases:

>100_bases
GGGTTAGCTCCGGTAATTATGGTCAACAATGATACTTACGGCCGGCTTACCCCGGATAAGGTAAAAGAAATTCTTGCCAA
GTACGAATAGGGGGTGCCTT

Downstream 100 bases:

>100_bases
AGGGGGGATACGGATGGCAGACGTTACTTTAAAAATAAACGGTATAGAAGTGACGGTGCCCGAAGGCACGACGATTTTGG
AAGCGGCTCAAATTGCTGGC

Product: Fe-hydrogenase subunit beta

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MRKVLICAGTGCISSGSPKVTEKFREELSKRGITDIEVYNTGCHGFCEQGPIVIIEPDKTFYCRVEVDDVPEIIEKHLLG
GQIVEKVLFHEPQVDKKVEKYTDISFYAKQKRLVLHNCGHINPERIEDYVANGGYEALRKVLTSMSPQEVVDEIKKSGLR
GRGGGGFPTGLKWQFTRDAKGDKKYVVCNADEGDPGAFMDRSVLEGDPHAVLEGMAICGYAVGADEGYIYVRAEYPLAIK
RLQIAIKQAEEKGYLGENIFGSGFNFRIKIKAGAGAFVCGEETALLTSIEGNRGMPRPRPPFPAVKGLWGKPTNINNVET
FANVPLIIKNGADWYASMGTEGSKGTKIFALTGKVNNTGLVEVPMGITMRQIIFDIGGGIKGGRKFKAVQIGGPSGGCLP
EELLDLPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVAKFFLRFTQKESCGKCVPCREGTKRMLEILEKITEGKGEPGDL
ETLEKLAYNIKNSSLCGLGQTAPNPVLSTLRYFRHEYEAHIYDKKCPAGVCAALLEYKILEDKCVGCGACARVCPVGAIS
GERKQPHQIDPEKCIKCGSCMEKCKFGAIVKG

Sequences:

>Translated_592_residues
MRKVLICAGTGCISSGSPKVTEKFREELSKRGITDIEVYNTGCHGFCEQGPIVIIEPDKTFYCRVEVDDVPEIIEKHLLG
GQIVEKVLFHEPQVDKKVEKYTDISFYAKQKRLVLHNCGHINPERIEDYVANGGYEALRKVLTSMSPQEVVDEIKKSGLR
GRGGGGFPTGLKWQFTRDAKGDKKYVVCNADEGDPGAFMDRSVLEGDPHAVLEGMAICGYAVGADEGYIYVRAEYPLAIK
RLQIAIKQAEEKGYLGENIFGSGFNFRIKIKAGAGAFVCGEETALLTSIEGNRGMPRPRPPFPAVKGLWGKPTNINNVET
FANVPLIIKNGADWYASMGTEGSKGTKIFALTGKVNNTGLVEVPMGITMRQIIFDIGGGIKGGRKFKAVQIGGPSGGCLP
EELLDLPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVAKFFLRFTQKESCGKCVPCREGTKRMLEILEKITEGKGEPGDL
ETLEKLAYNIKNSSLCGLGQTAPNPVLSTLRYFRHEYEAHIYDKKCPAGVCAALLEYKILEDKCVGCGACARVCPVGAIS
GERKQPHQIDPEKCIKCGSCMEKCKFGAIVKG
>Mature_592_residues
MRKVLICAGTGCISSGSPKVTEKFREELSKRGITDIEVYNTGCHGFCEQGPIVIIEPDKTFYCRVEVDDVPEIIEKHLLG
GQIVEKVLFHEPQVDKKVEKYTDISFYAKQKRLVLHNCGHINPERIEDYVANGGYEALRKVLTSMSPQEVVDEIKKSGLR
GRGGGGFPTGLKWQFTRDAKGDKKYVVCNADEGDPGAFMDRSVLEGDPHAVLEGMAICGYAVGADEGYIYVRAEYPLAIK
RLQIAIKQAEEKGYLGENIFGSGFNFRIKIKAGAGAFVCGEETALLTSIEGNRGMPRPRPPFPAVKGLWGKPTNINNVET
FANVPLIIKNGADWYASMGTEGSKGTKIFALTGKVNNTGLVEVPMGITMRQIIFDIGGGIKGGRKFKAVQIGGPSGGCLP
EELLDLPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVAKFFLRFTQKESCGKCVPCREGTKRMLEILEKITEGKGEPGDL
ETLEKLAYNIKNSSLCGLGQTAPNPVLSTLRYFRHEYEAHIYDKKCPAGVCAALLEYKILEDKCVGCGACARVCPVGAIS
GERKQPHQIDPEKCIKCGSCMEKCKFGAIVKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=452, Percent_Identity=38.0530973451327, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI260656005, Length=400, Percent_Identity=40.75, Blast_Score=321, Evalue=1e-87,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=44.7761194029851, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17531727, Length=400, Percent_Identity=40.25, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=41.3333333333333, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI45550452, Length=390, Percent_Identity=40.7692307692308, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24653819, Length=385, Percent_Identity=34.2857142857143, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=32.6760563380282, Blast_Score=208, Evalue=9e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 64450; Mature: 64450

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVLICAGTGCISSGSPKVTEKFREELSKRGITDIEVYNTGCHGFCEQGPIVIIEPDKT
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCE
FYCRVEVDDVPEIIEKHLLGGQIVEKVLFHEPQVDKKVEKYTDISFYAKQKRLVLHNCGH
EEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCEEEECCCC
INPERIEDYVANGGYEALRKVLTSMSPQEVVDEIKKSGLRGRGGGGFPTGLKWQFTRDAK
CCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCC
GDKKYVVCNADEGDPGAFMDRSVLEGDPHAVLEGMAICGYAVGADEGYIYVRAEYPLAIK
CCCEEEEEECCCCCCCCHHCCHHHCCCCHHHHHHHHHHEEEECCCCCEEEEEECCCHHHH
RLQIAIKQAEEKGYLGENIFGSGFNFRIKIKAGAGAFVCGEETALLTSIEGNRGMPRPRP
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCEEEEEECCCCCCCCCCC
PFPAVKGLWGKPTNINNVETFANVPLIIKNGADWYASMGTEGSKGTKIFALTGKVNNTGL
CCHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHCCCCCCCCCEEEEEECCCCCCCE
VEVPMGITMRQIIFDIGGGIKGGRKFKAVQIGGPSGGCLPEELLDLPVDYDSLIKAGAMM
EEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHEE
GSGGLVVMDETTCMVDVAKFFLRFTQKESCGKCVPCREGTKRMLEILEKITEGKGEPGDL
CCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCH
ETLEKLAYNIKNSSLCGLGQTAPNPVLSTLRYFRHEYEAHIYDKKCPAGVCAALLEYKIL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EDKCVGCGACARVCPVGAISGERKQPHQIDPEKCIKCGSCMEKCKFGAIVKG
HHHCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRKVLICAGTGCISSGSPKVTEKFREELSKRGITDIEVYNTGCHGFCEQGPIVIIEPDKT
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCE
FYCRVEVDDVPEIIEKHLLGGQIVEKVLFHEPQVDKKVEKYTDISFYAKQKRLVLHNCGH
EEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCEEEECCCC
INPERIEDYVANGGYEALRKVLTSMSPQEVVDEIKKSGLRGRGGGGFPTGLKWQFTRDAK
CCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCC
GDKKYVVCNADEGDPGAFMDRSVLEGDPHAVLEGMAICGYAVGADEGYIYVRAEYPLAIK
CCCEEEEEECCCCCCCCHHCCHHHCCCCHHHHHHHHHHEEEECCCCCEEEEEECCCHHHH
RLQIAIKQAEEKGYLGENIFGSGFNFRIKIKAGAGAFVCGEETALLTSIEGNRGMPRPRP
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCEEEEEECCCCCCCCCCC
PFPAVKGLWGKPTNINNVETFANVPLIIKNGADWYASMGTEGSKGTKIFALTGKVNNTGL
CCHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHCCCCCCCCCEEEEEECCCCCCCE
VEVPMGITMRQIIFDIGGGIKGGRKFKAVQIGGPSGGCLPEELLDLPVDYDSLIKAGAMM
EEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHEE
GSGGLVVMDETTCMVDVAKFFLRFTQKESCGKCVPCREGTKRMLEILEKITEGKGEPGDL
CCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCH
ETLEKLAYNIKNSSLCGLGQTAPNPVLSTLRYFRHEYEAHIYDKKCPAGVCAALLEYKIL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EDKCVGCGACARVCPVGAISGERKQPHQIDPEKCIKCGSCMEKCKFGAIVKG
HHHCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]