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The map label for this gene is proB

Identifier: 78043896

GI number: 78043896

Start: 324500

End: 325618

Strand: Direct

Name: proB

Synonym: CHY_0380

Alternate gene names: 78043896

Gene position: 324500-325618 (Clockwise)

Preceding gene: 78043770

Following gene: 78044812

Centisome position: 13.51

GC content: 46.65

Gene sequence:

>1119_bases
ATGCGCGAAATATTAAAAAATGTCCGCCGGCTGGTTGTAAAAATCGGTTCTTCGTCCTTAACCCACCCCGAGACGGGGAA
AATTAACCTTGCGGCTATGGAGCGTTTTGTAAGGGAATGTGCCGATTTAAAAAATGCAGGATTCGAAGTAATTATTGTCT
CTTCCGGAGCAATAGCTGCCGGAATGGGAAGGCTTGCCCTTCCAGAAAAGCCGAAAAATTTACCGGAAAAACAAGCATGT
GCTGCGGTAGGCCAGGGGGTATTAATGCATTTATACGAAAAATTTTTTTCCGAATACCAGGTGATCGCAGCGCAGGTGCT
TTTAACCGGGGAAGATTTGGCGGTAAGGAAGCGTTTTTTGAACGCCAAACATACTTTTTCGGCCCTTTTAAATTACGGGG
TTGTTCCGGTGGTTAATGAAAACGATACGGTAGCGGTAGAGGAAATTCGCTTTGGGGATAACGATACATTATCGGCCCGG
GTAGCGGTACTGGTGGAGGCTGATCTCCTGGTTCTTCTTTCCGATATCGATGGCTTGTATACCGCAGACCCCAGGAAGAA
TAGTAATGCCCGTTTTCTTCCGGAGGTTTTGGAAATTAATGAGGAAATCGAACAGCTGGCCGGGGGAAGCGGGACGGCAG
TTGGTACCGGTGGGATGGAGACCAAAATAGAGGCGGCGAAAATAGCGGTAAATGCGGGGATACCCATGGTAATTGCCCGG
GCAGATTATGGCAATTTGCGGCGAATTTTAAGAGGGGAAGAGGTTGGAACCTTTTTTTACCCGAAAAAGAAAAAGCAGTG
GAAAAAGCAATGGCTGTTATCCGGGGCAAGAGTGCAGGGAAGTATCATAGTTGATTTGGGAGCTGAAGAAGCCCTTTGCA
GTGGCGGAAAGAGCCTTTTGCCTTCGGGAGTGCTGGGAGTGGAAGGAGAATTTCCGACAGGAGCTATAGTTGCGGTTAAA
AATTTAAAGGGACGGGTGATTGCTAAAGGGCTTACCAATTATGCGGCTCAGGATATCTTAAAAATTAAGGGACTTCATTC
CTGGGAAATTGCTGACGTTTTAGGCCATAAAGATTATGACGAAATTATTCACCGGGATAATCTGGTGCTGGTGGATTAA

Upstream 100 bases:

>100_bases
GCTGCGGCAGGTTCGCCCAACTCAGCTGTACCAATAACGGTATCAAGGCTTTTAGAAATAACCGGAGGCCAAAAATGCCG
GGTAACGGAGTGAGTTAACA

Downstream 100 bases:

>100_bases
GGAGGTAAGCAGCAGCTTCGATAAATTGCACTGTAAGTGACTGTGTTTAACTGTTTTGTTATCATAAAAGTATGGAACTC
TTATCCATTGGAGAAGTGGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MREILKNVRRLVVKIGSSSLTHPETGKINLAAMERFVRECADLKNAGFEVIIVSSGAIAAGMGRLALPEKPKNLPEKQAC
AAVGQGVLMHLYEKFFSEYQVIAAQVLLTGEDLAVRKRFLNAKHTFSALLNYGVVPVVNENDTVAVEEIRFGDNDTLSAR
VAVLVEADLLVLLSDIDGLYTADPRKNSNARFLPEVLEINEEIEQLAGGSGTAVGTGGMETKIEAAKIAVNAGIPMVIAR
ADYGNLRRILRGEEVGTFFYPKKKKQWKKQWLLSGARVQGSIIVDLGAEEALCSGGKSLLPSGVLGVEGEFPTGAIVAVK
NLKGRVIAKGLTNYAAQDILKIKGLHSWEIADVLGHKDYDEIIHRDNLVLVD

Sequences:

>Translated_372_residues
MREILKNVRRLVVKIGSSSLTHPETGKINLAAMERFVRECADLKNAGFEVIIVSSGAIAAGMGRLALPEKPKNLPEKQAC
AAVGQGVLMHLYEKFFSEYQVIAAQVLLTGEDLAVRKRFLNAKHTFSALLNYGVVPVVNENDTVAVEEIRFGDNDTLSAR
VAVLVEADLLVLLSDIDGLYTADPRKNSNARFLPEVLEINEEIEQLAGGSGTAVGTGGMETKIEAAKIAVNAGIPMVIAR
ADYGNLRRILRGEEVGTFFYPKKKKQWKKQWLLSGARVQGSIIVDLGAEEALCSGGKSLLPSGVLGVEGEFPTGAIVAVK
NLKGRVIAKGLTNYAAQDILKIKGLHSWEIADVLGHKDYDEIIHRDNLVLVD
>Mature_372_residues
MREILKNVRRLVVKIGSSSLTHPETGKINLAAMERFVRECADLKNAGFEVIIVSSGAIAAGMGRLALPEKPKNLPEKQAC
AAVGQGVLMHLYEKFFSEYQVIAAQVLLTGEDLAVRKRFLNAKHTFSALLNYGVVPVVNENDTVAVEEIRFGDNDTLSAR
VAVLVEADLLVLLSDIDGLYTADPRKNSNARFLPEVLEINEEIEQLAGGSGTAVGTGGMETKIEAAKIAVNAGIPMVIAR
ADYGNLRRILRGEEVGTFFYPKKKKQWKKQWLLSGARVQGSIIVDLGAEEALCSGGKSLLPSGVLGVEGEFPTGAIVAVK
NLKGRVIAKGLTNYAAQDILKIKGLHSWEIADVLGHKDYDEIIHRDNLVLVD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=293, Percent_Identity=34.1296928327645, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI62912457, Length=291, Percent_Identity=34.020618556701, Blast_Score=148, Evalue=8e-36,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=43.5967302452316, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17569969, Length=278, Percent_Identity=33.8129496402878, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17569967, Length=278, Percent_Identity=33.8129496402878, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=37.6811594202899, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6321822, Length=415, Percent_Identity=37.3493975903614, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=34.8275862068965, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_CARHZ (Q3AF41)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359243.1
- ProteinModelPortal:   Q3AF41
- SMR:   Q3AF41
- STRING:   Q3AF41
- GeneID:   3726519
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0380
- NMPDR:   fig|246194.3.peg.597
- TIGR:   CHY_0380
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   CHYD246194:CHY_0380-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40297; Mature: 40297

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREILKNVRRLVVKIGSSSLTHPETGKINLAAMERFVRECADLKNAGFEVIIVSSGAIAA
CHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCHHH
GMGRLALPEKPKNLPEKQACAAVGQGVLMHLYEKFFSEYQVIAAQVLLTGEDLAVRKRFL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NAKHTFSALLNYGVVPVVNENDTVAVEEIRFGDNDTLSARVAVLVEADLLVLLSDIDGLY
CHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHCCCCE
TADPRKNSNARFLPEVLEINEEIEQLAGGSGTAVGTGGMETKIEAAKIAVNAGIPMVIAR
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEEEECCCCEEEEE
ADYGNLRRILRGEEVGTFFYPKKKKQWKKQWLLSGARVQGSIIVDLGAEEALCSGGKSLL
CCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCEECCEEEEEECCCHHHHCCCHHHC
PSGVLGVEGEFPTGAIVAVKNLKGRVIAKGLTNYAAQDILKIKGLHSWEIADVLGHKDYD
CCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHH
EIIHRDNLVLVD
HHHCCCCEEEEC
>Mature Secondary Structure
MREILKNVRRLVVKIGSSSLTHPETGKINLAAMERFVRECADLKNAGFEVIIVSSGAIAA
CHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCHHH
GMGRLALPEKPKNLPEKQACAAVGQGVLMHLYEKFFSEYQVIAAQVLLTGEDLAVRKRFL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NAKHTFSALLNYGVVPVVNENDTVAVEEIRFGDNDTLSARVAVLVEADLLVLLSDIDGLY
CHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHCCCCE
TADPRKNSNARFLPEVLEINEEIEQLAGGSGTAVGTGGMETKIEAAKIAVNAGIPMVIAR
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEEEECCCCEEEEE
ADYGNLRRILRGEEVGTFFYPKKKKQWKKQWLLSGARVQGSIIVDLGAEEALCSGGKSLL
CCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCEECCEEEEEECCCHHHHCCCHHHC
PSGVLGVEGEFPTGAIVAVKNLKGRVIAKGLTNYAAQDILKIKGLHSWEIADVLGHKDYD
CCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHH
EIIHRDNLVLVD
HHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA