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The map label for this gene is thiC

Identifier: 78043716

GI number: 78043716

Start: 658050

End: 659339

Strand: Direct

Name: thiC

Synonym: CHY_0747

Alternate gene names: 78043716

Gene position: 658050-659339 (Clockwise)

Preceding gene: 78044820

Following gene: 78042771

Centisome position: 27.4

GC content: 47.91

Gene sequence:

>1290_bases
ATGACTCAGCTTTTAAAAGCAAAAGAGGGAGTTATTACCAGGGAAATGGAAGTAGTGGCGGCGGAGGAAAGAAAATCGCC
GGAAGAAATCCGGCAAAAGGTAGCTTTGGGGGAAGTGGTAATACCCGCTAATGTTAATCACCAAAATTTGCATCCCAAAG
GTATTGGAGCGGGGCTTAAAGTAAAAGTAAATGCTAACCTTGGGACTTCGGAAAATCGCTGTTTTTACGAAGATGAACTA
AAAAAAGTAAAGGTGGCTATTAAAGCGGGAGCCGATGCAATAATGGACTTAAGTACCGGCGGAAATCTTGATGAAATTCG
AAGGGCGATTATAAAAGAAAGTTCGGTGCCGGTAGGGACCGTACCTTTATATCAGGCGGCGGCTGAAACGCTAAATAAAT
ACGGTGATATAAGTCGTTTGGACCCGGAACTCCTGTTTGACGTTATCGAAAAACAGGCAGCTGACGGCGTTGACTTTATG
ACGGTGCATGTGGGGGTGACCCGGGAAATCCTTAAGGTTCTTGACCGTTTTCCCAGGGTAACGGAGGTGGTAAGCCGGGG
AGGCTCTCTGACCATTGCCTGGATGGAGAAAAACGGAAGAGAAAATCCATTGTATGAACAGTTCGACCGGCTTTTAGCTA
TTTGCCGGAAATACGACGTTACCTTAAGCCTGGGGGACGGTTTAAGACCGGGAAGTATTGCGGATGCTACTGACCAGCTG
CAAATTATGGAATTAATGAAGCTCGGCGAACTGGTTAAGTATGCCCAAAATCAAGGCGTCCAGGTTATGGTGGAAGGCCC
CGGGCATGTGCCCATCAATCAAATTGAAATGAACGTTAAACTTATGAAGCGGCTTTGCGCCAATGCTCCCTTTTATGTAT
TAGGGCCACTGGTTACCGACATTGCTCCGGGATACGACCATATTACTGCGGCGATTGGTGGCGCCTGGGCTGCGTATTTT
GGGGCGGATTTCCTCTGCTACGTTACTCCAGCGGAGCATTTAGGATTGCCTACGGTGGAAGATGTAGAAGAAGGGGTGAT
TGCCTTAAAAATTGCGGCCCATGCAGCCGATTTGGCCCGGGGGAACCGGGAAGCCTGGAACCGGGATTATGAAATGAGTG
TAGCCCGAAAAGAGTTAAACTGGGAACGGCAGTTTGAACTGGCAATAAATCCCGAGCGGGCCAGGAAAATGCGAATAGAG
CGCGGCTCTCAAGACATAAAGAGCTGTTCCATGTGCGGGGAACTTTGTGCCATGAAAATTATGAATGAAAGGGGCGGAAA
AAATGCTTAA

Upstream 100 bases:

>100_bases
TGGGATTGCGGTAATTTCGGCTGTATTTGGAGCGGAAAGTCCGGCTCGGGCAGTTTATGAACTACGGGAAACAATAATTA
GAGCAAGGGAGTGAGCGATA

Downstream 100 bases:

>100_bases
CACCCTATGGGAAATCAGGGAGAAGGTCCGGGACTTATCTCCTCTGGTGGTAAATTTAACCAATAATGTGGTGACTAATT
TTACCGCAAATGTACTTTTA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTQLLKAKEGVITREMEVVAAEERKSPEEIRQKVALGEVVIPANVNHQNLHPKGIGAGLKVKVNANLGTSENRCFYEDEL
KKVKVAIKAGADAIMDLSTGGNLDEIRRAIIKESSVPVGTVPLYQAAAETLNKYGDISRLDPELLFDVIEKQAADGVDFM
TVHVGVTREILKVLDRFPRVTEVVSRGGSLTIAWMEKNGRENPLYEQFDRLLAICRKYDVTLSLGDGLRPGSIADATDQL
QIMELMKLGELVKYAQNQGVQVMVEGPGHVPINQIEMNVKLMKRLCANAPFYVLGPLVTDIAPGYDHITAAIGGAWAAYF
GADFLCYVTPAEHLGLPTVEDVEEGVIALKIAAHAADLARGNREAWNRDYEMSVARKELNWERQFELAINPERARKMRIE
RGSQDIKSCSMCGELCAMKIMNERGGKNA

Sequences:

>Translated_429_residues
MTQLLKAKEGVITREMEVVAAEERKSPEEIRQKVALGEVVIPANVNHQNLHPKGIGAGLKVKVNANLGTSENRCFYEDEL
KKVKVAIKAGADAIMDLSTGGNLDEIRRAIIKESSVPVGTVPLYQAAAETLNKYGDISRLDPELLFDVIEKQAADGVDFM
TVHVGVTREILKVLDRFPRVTEVVSRGGSLTIAWMEKNGRENPLYEQFDRLLAICRKYDVTLSLGDGLRPGSIADATDQL
QIMELMKLGELVKYAQNQGVQVMVEGPGHVPINQIEMNVKLMKRLCANAPFYVLGPLVTDIAPGYDHITAAIGGAWAAYF
GADFLCYVTPAEHLGLPTVEDVEEGVIALKIAAHAADLARGNREAWNRDYEMSVARKELNWERQFELAINPERARKMRIE
RGSQDIKSCSMCGELCAMKIMNERGGKNA
>Mature_428_residues
TQLLKAKEGVITREMEVVAAEERKSPEEIRQKVALGEVVIPANVNHQNLHPKGIGAGLKVKVNANLGTSENRCFYEDELK
KVKVAIKAGADAIMDLSTGGNLDEIRRAIIKESSVPVGTVPLYQAAAETLNKYGDISRLDPELLFDVIEKQAADGVDFMT
VHVGVTREILKVLDRFPRVTEVVSRGGSLTIAWMEKNGRENPLYEQFDRLLAICRKYDVTLSLGDGLRPGSIADATDQLQ
IMELMKLGELVKYAQNQGVQVMVEGPGHVPINQIEMNVKLMKRLCANAPFYVLGPLVTDIAPGYDHITAAIGGAWAAYFG
ADFLCYVTPAEHLGLPTVEDVEEGVIALKIAAHAADLARGNREAWNRDYEMSVARKELNWERQFELAINPERARKMRIER
GSQDIKSCSMCGELCAMKIMNERGGKNA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=456, Percent_Identity=46.4912280701754, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CARHZ (Q3AE33)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359601.1
- STRING:   Q3AE33
- GeneID:   3726669
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0747
- NMPDR:   fig|246194.3.peg.780
- TIGR:   CHY_0747
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   CHYD246194:CHY_0747-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47317; Mature: 47186

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLLKAKEGVITREMEVVAAEERKSPEEIRQKVALGEVVIPANVNHQNLHPKGIGAGLK
CCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEE
VKVNANLGTSENRCFYEDELKKVKVAIKAGADAIMDLSTGGNLDEIRRAIIKESSVPVGT
EEEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCC
VPLYQAAAETLNKYGDISRLDPELLFDVIEKQAADGVDFMTVHVGVTREILKVLDRFPRV
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCHH
TEVVSRGGSLTIAWMEKNGRENPLYEQFDRLLAICRKYDVTLSLGDGLRPGSIADATDQL
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHH
QIMELMKLGELVKYAQNQGVQVMVEGPGHVPINQIEMNVKLMKRLCANAPFYVLGPLVTD
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
IAPGYDHITAAIGGAWAAYFGADFLCYVTPAEHLGLPTVEDVEEGVIALKIAAHAADLAR
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHCCEEEEEHHHHHHHHHC
GNREAWNRDYEMSVARKELNWERQFELAINPERARKMRIERGSQDIKSCSMCGELCAMKI
CCHHHHCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
MNERGGKNA
HHHCCCCCC
>Mature Secondary Structure 
TQLLKAKEGVITREMEVVAAEERKSPEEIRQKVALGEVVIPANVNHQNLHPKGIGAGLK
CCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEE
VKVNANLGTSENRCFYEDELKKVKVAIKAGADAIMDLSTGGNLDEIRRAIIKESSVPVGT
EEEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCC
VPLYQAAAETLNKYGDISRLDPELLFDVIEKQAADGVDFMTVHVGVTREILKVLDRFPRV
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCHH
TEVVSRGGSLTIAWMEKNGRENPLYEQFDRLLAICRKYDVTLSLGDGLRPGSIADATDQL
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHH
QIMELMKLGELVKYAQNQGVQVMVEGPGHVPINQIEMNVKLMKRLCANAPFYVLGPLVTD
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
IAPGYDHITAAIGGAWAAYFGADFLCYVTPAEHLGLPTVEDVEEGVIALKIAAHAADLAR
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHCCEEEEEHHHHHHHHHC
GNREAWNRDYEMSVARKELNWERQFELAINPERARKMRIERGSQDIKSCSMCGELCAMKI
CCHHHHCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
MNERGGKNA
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA