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The map label for this gene is pth

Identifier: 78043715

GI number: 78043715

Start: 163814

End: 164377

Strand: Direct

Name: pth

Synonym: CHY_0196

Alternate gene names: 78043715

Gene position: 163814-164377 (Clockwise)

Preceding gene: 78042985

Following gene: 78043733

Centisome position: 6.82

GC content: 42.91

Gene sequence:

>564_bases
ATGTTTATCATAGCAGGATTGGGGAACCCCGGACAGGAATATGAAAATACCCGGCATAATGCCGGGTTTATGGTTGTAGA
TGAGTTAGCTAAAAAACATGGAATATTAATAACCAAAAGAAAATTTAAAAGCCTTGTCGGTGAAGGGGAAATTTTAGGGG
TAAAAGTTTTATTGCTTAAACCTCAAACCTATATGAATTTAAGCGGGACAGCAGTCCAGGAGGCGGTTTCTTTTTACAAA
CTCCCTCTGTCCCGCTTAGTTGTTGTTTATGATGATTTAGACTTGCCTTTAGGGAAAATTCGTCTTCGTTTAAAAGGGAG
TGCCGGCGGTCATCGGGGAATGGGGTCTATTATTAGTTGTCTGGGAAGTGAGGAAATACCCCGATTAAAAATTGGGATTG
GTCGACCGGCGGTGGGAGATGTTAAGGATTATGTTTTACAGCCCTTTACCGGTGCCGAGAGGGAGATTTTAGAGCCTACC
CTTAAACTGGCGGCGGAAGCCATAACCGTAGCTCTAACGGAAGGATTTAATAAAGCAATGACTGACTTTAACCGGGGAGG
TTAA

Upstream 100 bases:

>100_bases
GCGTTAATATCTGCTCCCAGGGATTATGTTGAAGAGGAAGAGGAAACAACTACCTCCGCCCCGGAAGCTACAGCTTAAAA
TTTTCGTAGGGAGAGGAAAA

Downstream 100 bases:

>100_bases
TAATTTACAAGGTGGCTTCTTTTTTTTATGGTTTTTTGGCAGCGGTGCTGGCTTATGGGGTAAATAAACTTTTAGTGCGC
TTGCTAACAAAGGAGAAGGT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MFIIAGLGNPGQEYENTRHNAGFMVVDELAKKHGILITKRKFKSLVGEGEILGVKVLLLKPQTYMNLSGTAVQEAVSFYK
LPLSRLVVVYDDLDLPLGKIRLRLKGSAGGHRGMGSIISCLGSEEIPRLKIGIGRPAVGDVKDYVLQPFTGAEREILEPT
LKLAAEAITVALTEGFNKAMTDFNRGG

Sequences:

>Translated_187_residues
MFIIAGLGNPGQEYENTRHNAGFMVVDELAKKHGILITKRKFKSLVGEGEILGVKVLLLKPQTYMNLSGTAVQEAVSFYK
LPLSRLVVVYDDLDLPLGKIRLRLKGSAGGHRGMGSIISCLGSEEIPRLKIGIGRPAVGDVKDYVLQPFTGAEREILEPT
LKLAAEAITVALTEGFNKAMTDFNRGG
>Mature_187_residues
MFIIAGLGNPGQEYENTRHNAGFMVVDELAKKHGILITKRKFKSLVGEGEILGVKVLLLKPQTYMNLSGTAVQEAVSFYK
LPLSRLVVVYDDLDLPLGKIRLRLKGSAGGHRGMGSIISCLGSEEIPRLKIGIGRPAVGDVKDYVLQPFTGAEREILEPT
LKLAAEAITVALTEGFNKAMTDFNRGG

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=177, Percent_Identity=37.2881355932203, Blast_Score=116, Evalue=1e-26,
Organism=Escherichia coli, GI1787455, Length=182, Percent_Identity=40.1098901098901, Blast_Score=100, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321983, Length=188, Percent_Identity=31.3829787234043, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_CARHZ (Q9F8Q3)

Other databases:

- EMBL:   AF244580
- EMBL:   CP000141
- RefSeq:   YP_359068.1
- ProteinModelPortal:   Q9F8Q3
- SMR:   Q9F8Q3
- STRING:   Q9F8Q3
- GeneID:   3726668
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0196
- NMPDR:   fig|246194.3.peg.1015
- TIGR:   CHY_0196
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   CHYD246194:CHY_0196-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20267; Mature: 20267

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIIAGLGNPGQEYENTRHNAGFMVVDELAKKHGILITKRKFKSLVGEGEILGVKVLLLK
CEEEEECCCCCHHHHCCCCCCCEEHHHHHHHHCCEEEEHHHHHHHHCCCCEEEEEEEEEC
PQTYMNLSGTAVQEAVSFYKLPLSRLVVVYDDLDLPLGKIRLRLKGSAGGHRGMGSIISC
CCHHCCCCCHHHHHHHHHHHCCHHHEEEEEECCCCCHHHEEEEEECCCCCCCCHHHHHHH
LGSEEIPRLKIGIGRPAVGDVKDYVLQPFTGAEREILEPTLKLAAEAITVALTEGFNKAM
HCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
TDFNRGG
HHCCCCC
>Mature Secondary Structure
MFIIAGLGNPGQEYENTRHNAGFMVVDELAKKHGILITKRKFKSLVGEGEILGVKVLLLK
CEEEEECCCCCHHHHCCCCCCCEEHHHHHHHHCCEEEEHHHHHHHHCCCCEEEEEEEEEC
PQTYMNLSGTAVQEAVSFYKLPLSRLVVVYDDLDLPLGKIRLRLKGSAGGHRGMGSIISC
CCHHCCCCCHHHHHHHHHHHCCHHHEEEEEECCCCCHHHEEEEEECCCCCCCCHHHHHHH
LGSEEIPRLKIGIGRPAVGDVKDYVLQPFTGAEREILEPTLKLAAEAITVALTEGFNKAM
HCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
TDFNRGG
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA