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The map label for this gene is mrdA [H]

Identifier: 78043714

GI number: 78043714

Start: 299740

End: 301692

Strand: Direct

Name: mrdA [H]

Synonym: CHY_0345

Alternate gene names: 78043714

Gene position: 299740-301692 (Clockwise)

Preceding gene: 78043106

Following gene: 78043204

Centisome position: 12.48

GC content: 46.34

Gene sequence:

>1953_bases
ATGGGTGTGGTGGTAACGTTTTTCCTGTTTATTTTATGGGGAAGGCTTTTTTATCTTCAAGTATACGCTACGGCCAAATA
TCAGACCTTGGCCCGCAAAAACGCCATCAGGATAATTGATGTTAAAGCCCCCCGGGGAGAAATATTGGACCGGAATGGCA
AAAAAATAGTTACCAATAAAGCCGTTTATACCGTTTCTTTACTGTTTACCGGCTTAAAAAATGCCGATGTAATTTTACCC
AAACTTGCGCAAATCCTTCAGGTTTCCGAAGAGGAACTGCGGTTAAAAATTAAAAACTCTAAAGCACGGCCGTATGAACC
GATTCGTTTAGCTACCGATGTTCCCTGGGAGGTGGTAGCGAAAATTGAAGAGAGAAGGTACGAACTTCCGGGAGTTACGG
TAAGCATCGAGCCCGCCCGGGATTATCCTTACGGTAATTTATTGGCTCACGTGGTGGGATATATTCAGGAGATAAAAGAT
ACCCAGCTAAAAGAGCATCAGGCCGAAGGATACAAGTTGGGGGATATGTTCGGACAAACGGGCTTAGAGAGTTTTTATGA
ATCGGTTTTGCGCGGAAAAAATGGTGGTCGGGAAATTGAAGTGGATGCCAAGGGCAATCCTGTCCGGGACTTAGGGATAA
AAACCCGACCGGAGCAGGGTAATTCCTTAGTTTTAACAATTGATGTAGACCTGCAAAAAGCTGCGGAAGAAAGTTTGAAA
AAAACCATTGCAGCGCTGCAAAATCGTTATCCCGATGCCAGAGCGGGTGCGGTAGTGGCCCTTGATGTGAGAAGCGGGGA
AGTGCTGGCCATGGCCAGTTACCCCACTTTTGATCCGGCGGCTTTTCCCAAGGGGTTAACCCAAAAGCAGGTGGATGAGT
ACATAAGGAATCCCGATCGGCCGCTTTATAATCGCGCGGTATCAGCTACCTATCCTCCAGGCTCCACCTTTAAAATGGTC
ACCGGCATGGCTGCCCTCTTAAATCGGGTAACTACTCCTTCCGAAACAATTTTCGATCCTGGTTACTACAAACTGGGAAA
CCGTGCTTTTAAAGATTGGAACCCAAGGGGCCACGGGGTTGTAAACTTTGTTAAAGCCATTAAAGAGTCTTGCAACGTTT
ATTTTTGGACGATGGCCCGGCGGCTGGGAGTAGAAAAAATTGTGGAAGTGGGTAAACAATTTGGTTTGGGAATGATGACA
GGCATAGACCTTCCCGGGGAGAGTTCGGGACTTTTACCGACTCCCGAGTGGAAAAAGGAAACCAATACTGCCATTATCGA
GAAAAATTTAAAGCCTAAAATAGATAAAATAAAAAGTGATTACGAGCAAAGAATAGCCCGGGCTAAAACTTCCGAGGAGA
AAAAGCGGTTGGAGGCGGAACGGGACAAAAAAATTCAGGCAATTAACCGGCAAATTGAAATTTACCGCTGGGAAACCGAG
TGGCAGCAGTATGATACGCTTAATATGGCCATCGGTCAGGGTTATAACAATTATACCCTTTTGCAGCTTGCCAATTATGT
AGCCATGATAGCCAATGGGGGAATTCGTTACCAACCTCATTTGGTAAAAAAAGTTTTGGACAATCGGGGAAAGGTTATAG
AGGAGGTAAAACCCAGGGTTTTAAGTAAAGCAAAGATCCCCGAGAATATTTTAAATTACGTGCGGCAGGGGATGCGGGAA
GTAACGATGCCCGGGGGAACTGCCGGAGGAGTGTTTGCAGGATTTCCGGTAGCGGTGGCGGCAAAAACCGGAACAGCTCA
GGTTTTCGGTAAAGACGATCACGGCTTGTTCGTGGCGTATGCCCCTTTTGAAAATCCCCGAATTGCAGTTGCGGCCATTA
TTGAACATGGCGGTCATGGTGGTTCTTCGGCAGGTTTGGTGGCCAGGGATGTTTTAGCGGCGTATTTTAAGGTGCCAAAA
GTAAATACCAGGGTTTTTGGCCCGGTGGAATAA

Upstream 100 bases:

>100_bases
TATACAATTTTTTCCTAACACCCGTCTTTTATTTCTTTTTTTACCGCGCCGTTAGGGGGAATAAGCCTTGAGAAAGAACA
TTAAAGCCAATTTAGGCTGG

Downstream 100 bases:

>100_bases
GAGCAGGAATTTTTCCAAAGATGTAGAATACATCAACTTAGCTAATTTTAAAGAAAAGGTGCTTTACGATGTTGAGAGGA
GAGTTTTTCTTAGAAAATAC

Product: peptidoglycan biosynthesis transpeptidase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MGVVVTFFLFILWGRLFYLQVYATAKYQTLARKNAIRIIDVKAPRGEILDRNGKKIVTNKAVYTVSLLFTGLKNADVILP
KLAQILQVSEEELRLKIKNSKARPYEPIRLATDVPWEVVAKIEERRYELPGVTVSIEPARDYPYGNLLAHVVGYIQEIKD
TQLKEHQAEGYKLGDMFGQTGLESFYESVLRGKNGGREIEVDAKGNPVRDLGIKTRPEQGNSLVLTIDVDLQKAAEESLK
KTIAALQNRYPDARAGAVVALDVRSGEVLAMASYPTFDPAAFPKGLTQKQVDEYIRNPDRPLYNRAVSATYPPGSTFKMV
TGMAALLNRVTTPSETIFDPGYYKLGNRAFKDWNPRGHGVVNFVKAIKESCNVYFWTMARRLGVEKIVEVGKQFGLGMMT
GIDLPGESSGLLPTPEWKKETNTAIIEKNLKPKIDKIKSDYEQRIARAKTSEEKKRLEAERDKKIQAINRQIEIYRWETE
WQQYDTLNMAIGQGYNNYTLLQLANYVAMIANGGIRYQPHLVKKVLDNRGKVIEEVKPRVLSKAKIPENILNYVRQGMRE
VTMPGGTAGGVFAGFPVAVAAKTGTAQVFGKDDHGLFVAYAPFENPRIAVAAIIEHGGHGGSSAGLVARDVLAAYFKVPK
VNTRVFGPVE

Sequences:

>Translated_650_residues
MGVVVTFFLFILWGRLFYLQVYATAKYQTLARKNAIRIIDVKAPRGEILDRNGKKIVTNKAVYTVSLLFTGLKNADVILP
KLAQILQVSEEELRLKIKNSKARPYEPIRLATDVPWEVVAKIEERRYELPGVTVSIEPARDYPYGNLLAHVVGYIQEIKD
TQLKEHQAEGYKLGDMFGQTGLESFYESVLRGKNGGREIEVDAKGNPVRDLGIKTRPEQGNSLVLTIDVDLQKAAEESLK
KTIAALQNRYPDARAGAVVALDVRSGEVLAMASYPTFDPAAFPKGLTQKQVDEYIRNPDRPLYNRAVSATYPPGSTFKMV
TGMAALLNRVTTPSETIFDPGYYKLGNRAFKDWNPRGHGVVNFVKAIKESCNVYFWTMARRLGVEKIVEVGKQFGLGMMT
GIDLPGESSGLLPTPEWKKETNTAIIEKNLKPKIDKIKSDYEQRIARAKTSEEKKRLEAERDKKIQAINRQIEIYRWETE
WQQYDTLNMAIGQGYNNYTLLQLANYVAMIANGGIRYQPHLVKKVLDNRGKVIEEVKPRVLSKAKIPENILNYVRQGMRE
VTMPGGTAGGVFAGFPVAVAAKTGTAQVFGKDDHGLFVAYAPFENPRIAVAAIIEHGGHGGSSAGLVARDVLAAYFKVPK
VNTRVFGPVE
>Mature_649_residues
GVVVTFFLFILWGRLFYLQVYATAKYQTLARKNAIRIIDVKAPRGEILDRNGKKIVTNKAVYTVSLLFTGLKNADVILPK
LAQILQVSEEELRLKIKNSKARPYEPIRLATDVPWEVVAKIEERRYELPGVTVSIEPARDYPYGNLLAHVVGYIQEIKDT
QLKEHQAEGYKLGDMFGQTGLESFYESVLRGKNGGREIEVDAKGNPVRDLGIKTRPEQGNSLVLTIDVDLQKAAEESLKK
TIAALQNRYPDARAGAVVALDVRSGEVLAMASYPTFDPAAFPKGLTQKQVDEYIRNPDRPLYNRAVSATYPPGSTFKMVT
GMAALLNRVTTPSETIFDPGYYKLGNRAFKDWNPRGHGVVNFVKAIKESCNVYFWTMARRLGVEKIVEVGKQFGLGMMTG
IDLPGESSGLLPTPEWKKETNTAIIEKNLKPKIDKIKSDYEQRIARAKTSEEKKRLEAERDKKIQAINRQIEIYRWETEW
QQYDTLNMAIGQGYNNYTLLQLANYVAMIANGGIRYQPHLVKKVLDNRGKVIEEVKPRVLSKAKIPENILNYVRQGMREV
TMPGGTAGGVFAGFPVAVAAKTGTAQVFGKDDHGLFVAYAPFENPRIAVAAIIEHGGHGGSSAGLVARDVLAAYFKVPKV
NTRVFGPVE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=645, Percent_Identity=30.3875968992248, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1786272, Length=419, Percent_Identity=26.2529832935561, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 72490; Mature: 72358

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVVTFFLFILWGRLFYLQVYATAKYQTLARKNAIRIIDVKAPRGEILDRNGKKIVTNK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECC
AVYTVSLLFTGLKNADVILPKLAQILQVSEEELRLKIKNSKARPYEPIRLATDVPWEVVA
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHEEEEECCCCCCCCCEEEECCCCHHHHH
KIEERRYELPGVTVSIEPARDYPYGNLLAHVVGYIQEIKDTQLKEHQAEGYKLGDMFGQT
HHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GLESFYESVLRGKNGGREIEVDAKGNPVRDLGIKTRPEQGNSLVLTIDVDLQKAAEESLK
HHHHHHHHHHCCCCCCCEEEECCCCCCCHHCCCEECCCCCCEEEEEEECCHHHHHHHHHH
KTIAALQNRYPDARAGAVVALDVRSGEVLAMASYPTFDPAAFPKGLTQKQVDEYIRNPDR
HHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHCCCCCCHHHHHHHHHCCCC
PLYNRAVSATYPPGSTFKMVTGMAALLNRVTTPSETIFDPGYYKLGNRAFKDWNPRGHGV
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHCCCCCCCCCCCCCHH
VNFVKAIKESCNVYFWTMARRLGVEKIVEVGKQFGLGMMTGIDLPGESSGLLPTPEWKKE
HHHHHHHHHHCCEEEEHHHHHHCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCC
TNTAIIEKNLKPKIDKIKSDYEQRIARAKTSEEKKRLEAERDKKIQAINRQIEIYRWETE
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECC
WQQYDTLNMAIGQGYNNYTLLQLANYVAMIANGGIRYQPHLVKKVLDNRGKVIEEVKPRV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCHHHHHHHHHH
LSKAKIPENILNYVRQGMREVTMPGGTAGGVFAGFPVAVAAKTGTAQVFGKDDHGLFVAY
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCEEEEECCCCEEEECCCCCCEEEEE
APFENPRIAVAAIIEHGGHGGSSAGLVARDVLAAYFKVPKVNTRVFGPVE
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GVVVTFFLFILWGRLFYLQVYATAKYQTLARKNAIRIIDVKAPRGEILDRNGKKIVTNK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECC
AVYTVSLLFTGLKNADVILPKLAQILQVSEEELRLKIKNSKARPYEPIRLATDVPWEVVA
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHEEEEECCCCCCCCCEEEECCCCHHHHH
KIEERRYELPGVTVSIEPARDYPYGNLLAHVVGYIQEIKDTQLKEHQAEGYKLGDMFGQT
HHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GLESFYESVLRGKNGGREIEVDAKGNPVRDLGIKTRPEQGNSLVLTIDVDLQKAAEESLK
HHHHHHHHHHCCCCCCCEEEECCCCCCCHHCCCEECCCCCCEEEEEEECCHHHHHHHHHH
KTIAALQNRYPDARAGAVVALDVRSGEVLAMASYPTFDPAAFPKGLTQKQVDEYIRNPDR
HHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHCCCCCCHHHHHHHHHCCCC
PLYNRAVSATYPPGSTFKMVTGMAALLNRVTTPSETIFDPGYYKLGNRAFKDWNPRGHGV
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHCCCCCCCCCCCCCHH
VNFVKAIKESCNVYFWTMARRLGVEKIVEVGKQFGLGMMTGIDLPGESSGLLPTPEWKKE
HHHHHHHHHHCCEEEEHHHHHHCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCC
TNTAIIEKNLKPKIDKIKSDYEQRIARAKTSEEKKRLEAERDKKIQAINRQIEIYRWETE
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECC
WQQYDTLNMAIGQGYNNYTLLQLANYVAMIANGGIRYQPHLVKKVLDNRGKVIEEVKPRV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCHHHHHHHHHH
LSKAKIPENILNYVRQGMREVTMPGGTAGGVFAGFPVAVAAKTGTAQVFGKDDHGLFVAY
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCEEEEECCCCEEEECCCCCCEEEEE
APFENPRIAVAAIIEHGGHGGSSAGLVARDVLAAYFKVPKVNTRVFGPVE
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]