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The map label for this gene is uvrB

Identifier: 78043646

GI number: 78043646

Start: 219819

End: 221807

Strand: Direct

Name: uvrB

Synonym: CHY_0258

Alternate gene names: 78043646

Gene position: 219819-221807 (Clockwise)

Preceding gene: 78043052

Following gene: 78044591

Centisome position: 9.15

GC content: 47.91

Gene sequence:

>1989_bases
ATGGAACGTTTTTTGTTAAAAGCAAATTATACGCCCAAGGGAGATCAACCCAAAGCCATAAAGGAACTTACCGAAGGGAT
TGAGAAGGGGCTAAAAATGCAGACCCTTCTGGGGGTAACCGGTTCTGGGAAAACTTTTACCATGGCCAATGTGATTGCCA
ATGTAAACAAGCCCACTTTAGTAATTGCACCCAATAAAACGCTGGCGGCCCAGCTTTGTGCGGAGTTCAGGGAGTTTTTT
CCCGAAAACGCGGTGGAGTATTTTGTAAGCTACTACGATTACTATCAACCGGAAGCTTACTTACCGGCCACCGATACGTA
TATAGAAAAAGACTCGGCTATAAACGATGAAATTGATAAACTCCGCCATTCAGCAACGGCGGCGCTTTTTGAACGGCGGG
ATGTTATTATCGTGGCATCGGTCTCCTGCATTTACGGTTTAGGGGATCCCCAGGAATATCGGGAACTTCTACTTTCCGTT
AGAAAAGGGCAGATTTACGAGCGGGAAGCAATTTTAAGAAAGCTTGTAGATATTCAGTATGAGCGGAATGAGTACGACTT
AACCCGGGGGAAATTTCGGGTAAGAGGGGATGTAATTGAAGTTTTTCCGGCGTCGTATACCGACCGGGCGGTGCGCATCG
AGCTTTTTGGCGATGAGGTGGATAGGATTTTAGAATTTGATACTTTAACGGGAGAGATTATCGGGGAGCTAAAACACGTG
GCCATTTTTCCGGCAAGCCACTTTGCTACTTCCAAAGAAAAGTTAGAACGGGCGATAAAAAGCATTGAAGAAGAGCTTGA
AGAACGGCTTCGCTATTTTGAAGAGCGGGGCAAACTCCTTGAAGCCCAGCGTTTAAAGCAGAGGACCTTGTACGATATTG
AAATGCTGCGGGAAGTAGGTTACACTAAAGGAATTGAAAACTATTCCCGGCATTTAACCGGAAGAAAACCCGGCGAGCCT
CCCTATACGTTAATTGATTATTTTCCCAAAGATTTTCTTATGATTATTGATGAAAGCCATATAACGATTCCCCAGATTCG
GGGGATGTATGAAGGGGACCGCTCCCGGAAGGAGGCGCTGGTGGAGTACGGTTTTCGCCTGCCTTCGGCCTTTGACAACC
GGCCACTGAAATTTCATGAGTTTGAAGCAAGAATTAACCAGGTAGTGTTTGTTTCGGCTACTCCCGGACCCTATGAGTTA
AAGCATTCTCAAAAGATTGTGGAGCAGATTATCCGGCCGACGGGGCTGGTTGACCCCGAGGTAGAAGTTCGACCCACCCG
GGGGCAGGTGGATGACCTCTACGGGGAAATTAAAGAGCGGGTAGCCCGAAACGAGCGGGTGCTGGTAACCACCCTCACCA
AGAAGATGGCCGAGGACCTGACCGAGTATTTCCGGGAGATGGGAGTGAAGGTCCGGTACCTTCATTCGGATATTGATACC
CTGGAACGGGTTGAAATCCTGCGAGACTTAAGGCTGGGAGTTTTTGATGTTTTGGTGGGAATAAACCTCCTCAGGGAAGG
TCTGGATTTACCCGAAGTTAGCCTGGTGGCCATTTTGGATGCCGACAAGGAAGGGTATTTACGTTCGGAACGCTCTTTAA
TCCAAACCATCGGACGGGCGGCGCGGAACGTAAACGGCAAAGTAATCATGTATGCCGATACCGTTACCGCTTCCATGCAA
AAAGCCATTGATGAAACCAACCGGCGCCGTAAACTTCAGATGGAATACAACCGGAAGCACGGAATAACGCCGCAAACGGT
GCAAAAGGCGGTAAGGGATGTTATTGAGGCGACCAGGGCGGTGACTGCAGAACTACCGGAAGTAAAGCGGGATTTCATCC
AGAAAATGAGTGCGAAAGAGTTTAAGCAGTATGTGGAAAAACTTACCCGGGAAATGAAAGAAGCAGCTAAAGCTTTGGAG
TTTGAAAAAGCGGCGATGCTGCGCGACTTAATAATTGAGCTTCGGGCGCAAAAGGCGGTCAAAAAGTAA

Upstream 100 bases:

>100_bases
TATGCCGGGGAAATTCTTTCCAAGCTGGCGGATATGTTAGGTGTTTAAGCAGGCCCGAAGGAACCCTTCGGGTTTTATAA
TATAGTTTGGGGTGTTTTGT

Downstream 100 bases:

>100_bases
TAATGGAGGTTTTACACACTGATGGATAAGATTGTCATTAAAGGGGCCAGGGTTCATAATCTAAAAAATATAGATTTAAC
CCTGCCGCGCAATAAATTTA

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MERFLLKANYTPKGDQPKAIKELTEGIEKGLKMQTLLGVTGSGKTFTMANVIANVNKPTLVIAPNKTLAAQLCAEFREFF
PENAVEYFVSYYDYYQPEAYLPATDTYIEKDSAINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPQEYRELLLSV
RKGQIYEREAILRKLVDIQYERNEYDLTRGKFRVRGDVIEVFPASYTDRAVRIELFGDEVDRILEFDTLTGEIIGELKHV
AIFPASHFATSKEKLERAIKSIEEELEERLRYFEERGKLLEAQRLKQRTLYDIEMLREVGYTKGIENYSRHLTGRKPGEP
PYTLIDYFPKDFLMIIDESHITIPQIRGMYEGDRSRKEALVEYGFRLPSAFDNRPLKFHEFEARINQVVFVSATPGPYEL
KHSQKIVEQIIRPTGLVDPEVEVRPTRGQVDDLYGEIKERVARNERVLVTTLTKKMAEDLTEYFREMGVKVRYLHSDIDT
LERVEILRDLRLGVFDVLVGINLLREGLDLPEVSLVAILDADKEGYLRSERSLIQTIGRAARNVNGKVIMYADTVTASMQ
KAIDETNRRRKLQMEYNRKHGITPQTVQKAVRDVIEATRAVTAELPEVKRDFIQKMSAKEFKQYVEKLTREMKEAAKALE
FEKAAMLRDLIIELRAQKAVKK

Sequences:

>Translated_662_residues
MERFLLKANYTPKGDQPKAIKELTEGIEKGLKMQTLLGVTGSGKTFTMANVIANVNKPTLVIAPNKTLAAQLCAEFREFF
PENAVEYFVSYYDYYQPEAYLPATDTYIEKDSAINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPQEYRELLLSV
RKGQIYEREAILRKLVDIQYERNEYDLTRGKFRVRGDVIEVFPASYTDRAVRIELFGDEVDRILEFDTLTGEIIGELKHV
AIFPASHFATSKEKLERAIKSIEEELEERLRYFEERGKLLEAQRLKQRTLYDIEMLREVGYTKGIENYSRHLTGRKPGEP
PYTLIDYFPKDFLMIIDESHITIPQIRGMYEGDRSRKEALVEYGFRLPSAFDNRPLKFHEFEARINQVVFVSATPGPYEL
KHSQKIVEQIIRPTGLVDPEVEVRPTRGQVDDLYGEIKERVARNERVLVTTLTKKMAEDLTEYFREMGVKVRYLHSDIDT
LERVEILRDLRLGVFDVLVGINLLREGLDLPEVSLVAILDADKEGYLRSERSLIQTIGRAARNVNGKVIMYADTVTASMQ
KAIDETNRRRKLQMEYNRKHGITPQTVQKAVRDVIEATRAVTAELPEVKRDFIQKMSAKEFKQYVEKLTREMKEAAKALE
FEKAAMLRDLIIELRAQKAVKK
>Mature_662_residues
MERFLLKANYTPKGDQPKAIKELTEGIEKGLKMQTLLGVTGSGKTFTMANVIANVNKPTLVIAPNKTLAAQLCAEFREFF
PENAVEYFVSYYDYYQPEAYLPATDTYIEKDSAINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPQEYRELLLSV
RKGQIYEREAILRKLVDIQYERNEYDLTRGKFRVRGDVIEVFPASYTDRAVRIELFGDEVDRILEFDTLTGEIIGELKHV
AIFPASHFATSKEKLERAIKSIEEELEERLRYFEERGKLLEAQRLKQRTLYDIEMLREVGYTKGIENYSRHLTGRKPGEP
PYTLIDYFPKDFLMIIDESHITIPQIRGMYEGDRSRKEALVEYGFRLPSAFDNRPLKFHEFEARINQVVFVSATPGPYEL
KHSQKIVEQIIRPTGLVDPEVEVRPTRGQVDDLYGEIKERVARNERVLVTTLTKKMAEDLTEYFREMGVKVRYLHSDIDT
LERVEILRDLRLGVFDVLVGINLLREGLDLPEVSLVAILDADKEGYLRSERSLIQTIGRAARNVNGKVIMYADTVTASMQ
KAIDETNRRRKLQMEYNRKHGITPQTVQKAVRDVIEATRAVTAELPEVKRDFIQKMSAKEFKQYVEKLTREMKEAAKALE
FEKAAMLRDLIIELRAQKAVKK

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=662, Percent_Identity=60.8761329305136, Blast_Score=806, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_CARHZ (Q3AFF4)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359130.1
- ProteinModelPortal:   Q3AFF4
- SMR:   Q3AFF4
- STRING:   Q3AFF4
- GeneID:   3728448
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0258
- NMPDR:   fig|246194.3.peg.231
- TIGR:   CHY_0258
- eggNOG:   COG0556
- HOGENOM:   HBG703949
- OMA:   AAQLCNE
- ProtClustDB:   PRK05298
- BioCyc:   CHYD246194:CHY_0258-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 76401; Mature: 76401

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFLLKANYTPKGDQPKAIKELTEGIEKGLKMQTLLGVTGSGKTFTMANVIANVNKPTL
CCCEEEECCCCCCCCCCHHHHHHHHHHHHCCEEHHHHEECCCCCEEEHHHHHHCCCCCEE
VIAPNKTLAAQLCAEFREFFPENAVEYFVSYYDYYQPEAYLPATDTYIEKDSAINDEIDK
EEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHHHH
LRHSATAALFERRDVIIVASVSCIYGLGDPQEYRELLLSVRKGQIYEREAILRKLVDIQY
HHHHHHHHHHHCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
ERNEYDLTRGKFRVRGDVIEVFPASYTDRAVRIELFGDEVDRILEFDTLTGEIIGELKHV
CCCCCCCCCCCEEEECCEEEECCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
AIFPASHFATSKEKLERAIKSIEEELEERLRYFEERGKLLEAQRLKQRTLYDIEMLREVG
EEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTKGIENYSRHLTGRKPGEPPYTLIDYFPKDFLMIIDESHITIPQIRGMYEGDRSRKEAL
HHCCHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCHHHHHHH
VEYGFRLPSAFDNRPLKFHEFEARINQVVFVSATPGPYELKHSQKIVEQIIRPTGLVDPE
HHHHCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
VEVRPTRGQVDDLYGEIKERVARNERVLVTTLTKKMAEDLTEYFREMGVKVRYLHSDIDT
CEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
LERVEILRDLRLGVFDVLVGINLLREGLDLPEVSLVAILDADKEGYLRSERSLIQTIGRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
ARNVNGKVIMYADTVTASMQKAIDETNRRRKLQMEYNRKHGITPQTVQKAVRDVIEATRA
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VTAELPEVKRDFIQKMSAKEFKQYVEKLTREMKEAAKALEFEKAAMLRDLIIELRAQKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KK
CC
>Mature Secondary Structure
MERFLLKANYTPKGDQPKAIKELTEGIEKGLKMQTLLGVTGSGKTFTMANVIANVNKPTL
CCCEEEECCCCCCCCCCHHHHHHHHHHHHCCEEHHHHEECCCCCEEEHHHHHHCCCCCEE
VIAPNKTLAAQLCAEFREFFPENAVEYFVSYYDYYQPEAYLPATDTYIEKDSAINDEIDK
EEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHHHH
LRHSATAALFERRDVIIVASVSCIYGLGDPQEYRELLLSVRKGQIYEREAILRKLVDIQY
HHHHHHHHHHHCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
ERNEYDLTRGKFRVRGDVIEVFPASYTDRAVRIELFGDEVDRILEFDTLTGEIIGELKHV
CCCCCCCCCCCEEEECCEEEECCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
AIFPASHFATSKEKLERAIKSIEEELEERLRYFEERGKLLEAQRLKQRTLYDIEMLREVG
EEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTKGIENYSRHLTGRKPGEPPYTLIDYFPKDFLMIIDESHITIPQIRGMYEGDRSRKEAL
HHCCHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCHHHHHHH
VEYGFRLPSAFDNRPLKFHEFEARINQVVFVSATPGPYELKHSQKIVEQIIRPTGLVDPE
HHHHCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
VEVRPTRGQVDDLYGEIKERVARNERVLVTTLTKKMAEDLTEYFREMGVKVRYLHSDIDT
CEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
LERVEILRDLRLGVFDVLVGINLLREGLDLPEVSLVAILDADKEGYLRSERSLIQTIGRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
ARNVNGKVIMYADTVTASMQKAIDETNRRRKLQMEYNRKHGITPQTVQKAVRDVIEATRA
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VTAELPEVKRDFIQKMSAKEFKQYVEKLTREMKEAAKALEFEKAAMLRDLIIELRAQKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA