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The map label for this gene is trxB [C]

Identifier: 78043252

GI number: 78043252

Start: 484181

End: 485461

Strand: Direct

Name: trxB [C]

Synonym: CHY_0560

Alternate gene names: 78043252

Gene position: 484181-485461 (Clockwise)

Preceding gene: 78043808

Following gene: 78043765

Centisome position: 20.16

GC content: 47.15

Gene sequence:

>1281_bases
ATGAGCGACAAAGTTATATATTTAAGCCAAGAACAGTTTGACGAAGTGGTATTAAAAAGCGATGTTCCGGTAATTGTGGA
TTTTTATTCCGAAGATTGTCCACCCTGCGAGGCACTGGCTCCGGCTTACGAGAAGCTTGCCCAGCAGTACGGGGATAAAT
TTAAGTTTGTCAAAATCTACCGGCAGCAAAACCGACCTTTAGCCGAAAAGCTGGGAGTTAAGGGTAGCCCCACAGTTCTG
TTTTATGTCAACGGGCAAGAAGTGGGCCAGAGGTTAACCGGCTATATTTCCAAGCGTCAGCTAAGGGAAGCCATGGAAAA
AGCTTTTGGCCAGAAACTTTTGCCGGAAGTTCCGGAAACGGTTAAGTATGATGTTATTGTTCTTGGTGGCGGTCCGGCAG
GTTTAAGTGCGGCTCTGTATACCGCCCGGGCCAAGTTAAGAACCATTGTGGTGGATGAAAGTGTTCCCGGTGGCCAGGCG
GCTACCACCTACCATATTGCTAACTACCCCGGTACCCCCGGAACGATTAGAGGTAAGGAACTAACTCAAAATATGCTTAA
TCAGGCCTTAGCTTTTGGTGCAGAAGTTGATGATTTAAAAGAAGTATTAAAAGTTGAATTAACCGGAGAGGTTAAGAGGG
TTATCACCGAAGATAAGATTTATGAAGCTCCGGCCATCATTTTAGCCACCGGAGCAGAACCAAGAAAACTTCCGGCGGAA
GGGGAAGACCTTTTCCGGGGCCGCGGCGTCCACTACTGTGCTACCTGTGATGGGGCAATGTACCAGGGCATGAAAGTAGT
GGTGGTTGGCGGTGGCAACTCAGCCGTAGAAGAAGCGGTATTTTTGACCCGTTTTGCCACCGAAGTTACGATTATTCACC
AGTTTGACCACTTCCAGGCTTCCAAAGTTGCCCAGGAAGAAGCTTTTGCCAATCCCAAAATAAAGGTTATCTGGGATTCG
GAAGTACGTAAGGTTGTTGGGGATAAACATGTAACCGGCGTTGTGATTGAAAATCTGAAAACCAAAGAACTTTCTACCGT
GCCTACCGATGGGGTGTTTGTTTACATTGGAACTCAGCCTAAAACCAATTTGTTTGCCGGACAGGTAGAGATGAACGAAT
GGGGTTATATTATTACCGATGAAGAAATGCGGACCAATATACCGGGAGTTTTTGCGGCGGGAGATTTAAGGCAGAAGTCA
GTACGGCAGGCGGTAACCGCTGCGGCCGATGGGGTAATTGCGGCAGTAAATGTGGAGAGATATCTTGCCAGTAAAAAGTA
A

Upstream 100 bases:

>100_bases
GTGTAAATCCGGGGGAGCAGCAAGTGGATGCCCGGACTGCGAGACCCTGGAAAAAATAAAGCAAAAGCTCTAAAAATTTA
AACTCAAGGGGGAGAGGAAA

Downstream 100 bases:

>100_bases
ATTTAGTATTATTGAAAAGGGGCACCTCCGCGGGTGCCCTTAAATAATAGTTTATTGGTATAATAAGATTAATACCCCCC
GTTAAACATAGATGTGTTAT

Product: thioredoxin/thioredoxin-disulfide reductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSDKVIYLSQEQFDEVVLKSDVPVIVDFYSEDCPPCEALAPAYEKLAQQYGDKFKFVKIYRQQNRPLAEKLGVKGSPTVL
FYVNGQEVGQRLTGYISKRQLREAMEKAFGQKLLPEVPETVKYDVIVLGGGPAGLSAALYTARAKLRTIVVDESVPGGQA
ATTYHIANYPGTPGTIRGKELTQNMLNQALAFGAEVDDLKEVLKVELTGEVKRVITEDKIYEAPAIILATGAEPRKLPAE
GEDLFRGRGVHYCATCDGAMYQGMKVVVVGGGNSAVEEAVFLTRFATEVTIIHQFDHFQASKVAQEEAFANPKIKVIWDS
EVRKVVGDKHVTGVVIENLKTKELSTVPTDGVFVYIGTQPKTNLFAGQVEMNEWGYIITDEEMRTNIPGVFAAGDLRQKS
VRQAVTAAADGVIAAVNVERYLASKK

Sequences:

>Translated_426_residues
MSDKVIYLSQEQFDEVVLKSDVPVIVDFYSEDCPPCEALAPAYEKLAQQYGDKFKFVKIYRQQNRPLAEKLGVKGSPTVL
FYVNGQEVGQRLTGYISKRQLREAMEKAFGQKLLPEVPETVKYDVIVLGGGPAGLSAALYTARAKLRTIVVDESVPGGQA
ATTYHIANYPGTPGTIRGKELTQNMLNQALAFGAEVDDLKEVLKVELTGEVKRVITEDKIYEAPAIILATGAEPRKLPAE
GEDLFRGRGVHYCATCDGAMYQGMKVVVVGGGNSAVEEAVFLTRFATEVTIIHQFDHFQASKVAQEEAFANPKIKVIWDS
EVRKVVGDKHVTGVVIENLKTKELSTVPTDGVFVYIGTQPKTNLFAGQVEMNEWGYIITDEEMRTNIPGVFAAGDLRQKS
VRQAVTAAADGVIAAVNVERYLASKK
>Mature_425_residues
SDKVIYLSQEQFDEVVLKSDVPVIVDFYSEDCPPCEALAPAYEKLAQQYGDKFKFVKIYRQQNRPLAEKLGVKGSPTVLF
YVNGQEVGQRLTGYISKRQLREAMEKAFGQKLLPEVPETVKYDVIVLGGGPAGLSAALYTARAKLRTIVVDESVPGGQAA
TTYHIANYPGTPGTIRGKELTQNMLNQALAFGAEVDDLKEVLKVELTGEVKRVITEDKIYEAPAIILATGAEPRKLPAEG
EDLFRGRGVHYCATCDGAMYQGMKVVVVGGGNSAVEEAVFLTRFATEVTIIHQFDHFQASKVAQEEAFANPKIKVIWDSE
VRKVVGDKHVTGVVIENLKTKELSTVPTDGVFVYIGTQPKTNLFAGQVEMNEWGYIITDEEMRTNIPGVFAAGDLRQKSV
RQAVTAAADGVIAAVNVERYLASKK

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=342, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI21389617, Length=342, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI226437568, Length=342, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=39.8089171974522, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87081763, Length=404, Percent_Identity=31.1881188118812, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082331, Length=106, Percent_Identity=41.5094339622642, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788936, Length=95, Percent_Identity=36.8421052631579, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320560, Length=317, Percent_Identity=37.8548895899054, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6321898, Length=319, Percent_Identity=37.3040752351097, Blast_Score=203, Evalue=4e-53,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 46676; Mature: 46545

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKVIYLSQEQFDEVVLKSDVPVIVDFYSEDCPPCEALAPAYEKLAQQYGDKFKFVKIY
CCCCEEEECHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEHHEE
RQQNRPLAEKLGVKGSPTVLFYVNGQEVGQRLTGYISKRQLREAMEKAFGQKLLPEVPET
HHCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VKYDVIVLGGGPAGLSAALYTARAKLRTIVVDESVPGGQAATTYHIANYPGTPGTIRGKE
CEEEEEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCHHH
LTQNMLNQALAFGAEVDDLKEVLKVELTGEVKRVITEDKIYEAPAIILATGAEPRKLPAE
HHHHHHHHHHHCCCCHHHHHHHHEEHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
GEDLFRGRGVHYCATCDGAMYQGMKVVVVGGGNSAVEEAVFLTRFATEVTIIHQFDHFQA
CHHHHHCCCEEEEEECCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHEEEEEEECCCHHH
SKVAQEEAFANPKIKVIWDSEVRKVVGDKHVTGVVIENLKTKELSTVPTDGVFVYIGTQP
HHHHHHHHHCCCCEEEEECHHHHHHHCCCCEEEHEECCCCCCCCCCCCCCCEEEEECCCC
KTNLFAGQVEMNEWGYIITDEEMRTNIPGVFAAGDLRQKSVRQAVTAAADGVIAAVNVER
CCCEEEEEEEECCCCEEEECHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEEHHH
YLASKK
HHCCCC
>Mature Secondary Structure 
SDKVIYLSQEQFDEVVLKSDVPVIVDFYSEDCPPCEALAPAYEKLAQQYGDKFKFVKIY
CCCEEEECHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEHHEE
RQQNRPLAEKLGVKGSPTVLFYVNGQEVGQRLTGYISKRQLREAMEKAFGQKLLPEVPET
HHCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VKYDVIVLGGGPAGLSAALYTARAKLRTIVVDESVPGGQAATTYHIANYPGTPGTIRGKE
CEEEEEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCHHH
LTQNMLNQALAFGAEVDDLKEVLKVELTGEVKRVITEDKIYEAPAIILATGAEPRKLPAE
HHHHHHHHHHHCCCCHHHHHHHHEEHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
GEDLFRGRGVHYCATCDGAMYQGMKVVVVGGGNSAVEEAVFLTRFATEVTIIHQFDHFQA
CHHHHHCCCEEEEEECCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHEEEEEEECCCHHH
SKVAQEEAFANPKIKVIWDSEVRKVVGDKHVTGVVIENLKTKELSTVPTDGVFVYIGTQP
HHHHHHHHHCCCCEEEEECHHHHHHHCCCCEEEHEECCCCCCCCCCCCCCCEEEEECCCC
KTNLFAGQVEMNEWGYIITDEEMRTNIPGVFAAGDLRQKSVRQAVTAAADGVIAAVNVER
CCCEEEEEEEECCCCEEEECHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEEHHH
YLASKK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]