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The map label for this gene is ddl

Identifier: 78043171

GI number: 78043171

Start: 1205125

End: 1206063

Strand: Direct

Name: ddl

Synonym: CHY_1346

Alternate gene names: 78043171

Gene position: 1205125-1206063 (Clockwise)

Preceding gene: 78045227

Following gene: 78044143

Centisome position: 50.18

GC content: 39.19

Gene sequence:

>939_bases
ATGAAAATTGGCGTTATTTGCGGCGGGCTTTCTTCCGAAAGAGAAGTTTCTCTACGTACCGGCGACGCGATTTTTAGAGC
TTTATTAAAAAAGGGATACAACGTTGTAAAAATCGATATGGACAGAAATATTGCCGAAACATTAAAAAAGGAAAATATCG
ATTTTGCCTTTATTGCCCTTCACGGGAAGTACGGGGAAGACGGAGCCATCCAGGGGCTACTTGAAATTATGGATATCCCC
TATACTGGCTCCGGAATACTTGCCAGTAGTTTAGCAATTGATAAAATAATGACAAAGAAAATTTTAAAAGCCGAGGGAAT
TCCGACACCAAACTATATTGCTTTTAGCTTTGATGAAAACCCCAATTTTGAAGCTATCAGTTCTGAAATACTGCAAACAT
TGAAACTTCCCGTGGTAATAAAAGCTCCCCGCGAAGGTTCAACTATTGGCATTGAGTTTGTTTTTAGCAAACAAGAGCTA
CCGAAAGCTATTAAAAAAGTTTTAGAAATAGATAAACAGCTTTTGGTTGAAGAATTTATTGAAGGGGTAGAAGTTACCGC
TTCAGTATTGGGCAATTCTAATCCCGTTGTTTTACCCTTAATTGAGATCGTCAGCAAGACTCGTTTTTACGATTACGAAG
CTAAATATACCCCAGGATTAAGTGAACATATCATACCTCCAAGAATAGCTCCGGAATTAAGTCAAAAAGCTATTGAATAC
GCCCGGAAAACGTATAAAGCTTTAGGTTGCCGCGGATTTGCGCGAGTGGATTTTATGATTGATGTAAGAAAAAATGAAGC
CTATGTCTTGGAAGTAAATACTATTCCGGGAATGACCGCCACCAGTTTATTTCCCGACGCAGCAAAAGCTCAGGGAATAA
GCTTTGAGGACCTGGTGGAAAAAATACTTATGCTCGGTTTAGAGGGGAGGCAAAAATAA

Upstream 100 bases:

>100_bases
TCAAGAAGAAGTTTTCAGGTAGAAATTTATTCCTATTTGTTATACGATTATAGTGTAATAAATAAAATATATTATAAAAC
ATTTTTTTGGAGGTAAAAAA

Downstream 100 bases:

>100_bases
AAATAGCATGAATAAATTATTTAAAATAATCTCGGAAATTGACAGGCTCGGATATAAAGCTTACTCTCGCCTTAAAGGTA
GTTACACTTTTCCGCAATTT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKIGVICGGLSSEREVSLRTGDAIFRALLKKGYNVVKIDMDRNIAETLKKENIDFAFIALHGKYGEDGAIQGLLEIMDIP
YTGSGILASSLAIDKIMTKKILKAEGIPTPNYIAFSFDENPNFEAISSEILQTLKLPVVIKAPREGSTIGIEFVFSKQEL
PKAIKKVLEIDKQLLVEEFIEGVEVTASVLGNSNPVVLPLIEIVSKTRFYDYEAKYTPGLSEHIIPPRIAPELSQKAIEY
ARKTYKALGCRGFARVDFMIDVRKNEAYVLEVNTIPGMTATSLFPDAAKAQGISFEDLVEKILMLGLEGRQK

Sequences:

>Translated_312_residues
MKIGVICGGLSSEREVSLRTGDAIFRALLKKGYNVVKIDMDRNIAETLKKENIDFAFIALHGKYGEDGAIQGLLEIMDIP
YTGSGILASSLAIDKIMTKKILKAEGIPTPNYIAFSFDENPNFEAISSEILQTLKLPVVIKAPREGSTIGIEFVFSKQEL
PKAIKKVLEIDKQLLVEEFIEGVEVTASVLGNSNPVVLPLIEIVSKTRFYDYEAKYTPGLSEHIIPPRIAPELSQKAIEY
ARKTYKALGCRGFARVDFMIDVRKNEAYVLEVNTIPGMTATSLFPDAAKAQGISFEDLVEKILMLGLEGRQK
>Mature_312_residues
MKIGVICGGLSSEREVSLRTGDAIFRALLKKGYNVVKIDMDRNIAETLKKENIDFAFIALHGKYGEDGAIQGLLEIMDIP
YTGSGILASSLAIDKIMTKKILKAEGIPTPNYIAFSFDENPNFEAISSEILQTLKLPVVIKAPREGSTIGIEFVFSKQEL
PKAIKKVLEIDKQLLVEEFIEGVEVTASVLGNSNPVVLPLIEIVSKTRFYDYEAKYTPGLSEHIIPPRIAPELSQKAIEY
ARKTYKALGCRGFARVDFMIDVRKNEAYVLEVNTIPGMTATSLFPDAAKAQGISFEDLVEKILMLGLEGRQK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=310, Percent_Identity=40, Blast_Score=220, Evalue=7e-59,
Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=30.8781869688385, Blast_Score=183, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_CARHZ (Q3ACF5)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_360179.1
- ProteinModelPortal:   Q3ACF5
- SMR:   Q3ACF5
- STRING:   Q3ACF5
- GeneID:   3728690
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1346
- NMPDR:   fig|246194.3.peg.1390
- TIGR:   CHY_1346
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- ProtClustDB:   PRK01372
- BioCyc:   CHYD246194:CHY_1346-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 34463; Mature: 34463

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVICGGLSSEREVSLRTGDAIFRALLKKGYNVVKIDMDRNIAETLKKENIDFAFIAL
CEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEEE
HGKYGEDGAIQGLLEIMDIPYTGSGILASSLAIDKIMTKKILKAEGIPTPNYIAFSFDEN
ECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
PNFEAISSEILQTLKLPVVIKAPREGSTIGIEFVFSKQELPKAIKKVLEIDKQLLVEEFI
CCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
EGVEVTASVLGNSNPVVLPLIEIVSKTRFYDYEAKYTPGLSEHIIPPRIAPELSQKAIEY
HHHHHHHHHHCCCCCEEEHHHHHHHHHHEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHH
ARKTYKALGCRGFARVDFMIDVRKNEAYVLEVNTIPGMTATSLFPDAAKAQGISFEDLVE
HHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCCHHHCCCCHHHHCCCCHHHHHH
KILMLGLEGRQK
HHHHHCCCCCCC
>Mature Secondary Structure
MKIGVICGGLSSEREVSLRTGDAIFRALLKKGYNVVKIDMDRNIAETLKKENIDFAFIAL
CEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEEE
HGKYGEDGAIQGLLEIMDIPYTGSGILASSLAIDKIMTKKILKAEGIPTPNYIAFSFDEN
ECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
PNFEAISSEILQTLKLPVVIKAPREGSTIGIEFVFSKQELPKAIKKVLEIDKQLLVEEFI
CCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
EGVEVTASVLGNSNPVVLPLIEIVSKTRFYDYEAKYTPGLSEHIIPPRIAPELSQKAIEY
HHHHHHHHHHCCCCCEEEHHHHHHHHHHEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHH
ARKTYKALGCRGFARVDFMIDVRKNEAYVLEVNTIPGMTATSLFPDAAKAQGISFEDLVE
HHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCCHHHCCCCHHHHCCCCHHHHHH
KILMLGLEGRQK
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA