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The map label for this gene is moaE [H]

Identifier: 78043166

GI number: 78043166

Start: 701074

End: 701745

Strand: Direct

Name: moaE [H]

Synonym: CHY_0792

Alternate gene names: 78043166

Gene position: 701074-701745 (Clockwise)

Preceding gene: 78044096

Following gene: 78042637

Centisome position: 29.19

GC content: 44.49

Gene sequence:

>672_bases
GTGCCCCGGAAGTATGCCCGTAATATCGGAAGTTTTACTTTAGCTGGGCAGAAAAAACTTTTAGCTTCCAAAGTAATGGT
TGTGGGCTTGGGCGGACTTGGTGGCTATGTTTTGGAAGAACTGTGTCGAGCAGGCGTGGGAGAAATAGTGGGGGTTGATG
GCGATGCCTTTGAGGAATCCAATTTAAACCGCCAGCTTTTGGCTACAGAAAAAAATCTTGGGCAGAAAAAAGCCAATAAA
GCCAGAAAAAGGGCGGCGGAGATCAATCAAACGGTGGTGGTAAGCGGTTTTGTAACCAAGGTCGAAAATCTTCCGGAAAC
TGCCTGGCAGGGAGTAGAGCTTGTCTTTGATTGTTTAGATAATATAGAAAGTCGTTTTTACCTCGAAGAAAAAACAAATA
ACCTTGGACTGCCCCTGGTGCACGGAGCAATAGGAGGCTGGTACGGCCAGGTCGGGATTGTTTGGCCCGGAAGTAACCTT
TTAACCAAGATTTACCGGGAACGGAAAAAAGGTATAGAACAAACCCTGGGGAACCCTCCTTTTACTCCGGCGGTGGCGGC
AAGTTTAATGGTAGCTTTGGGGATTAATTTGCTTCTGGGAACGTTGGAAAAAGAAAGCGTATTTTACTTTTTTGATTTAA
AAAATATGGAGTTTGAGAAAATAAGCTTTTAA

Upstream 100 bases:

>100_bases
AGGTATTAAGGAATTATCCCTGGAAACAACCGAGAGAATTTCCCGGGAAACCGGTATTGCTCTACGCGCTATTGAATATT
TTGCCCTCGAAAACGGGGTT

Downstream 100 bases:

>100_bases
AATTGTATTACCAATCACAAAGACTGCTTAATTTACCTTTTTCTTTTTTTCCTGAAAAACCAGTGTGTAAACAAATGTTT
ACAGATTGACAAGTTGATTT

Product: molybdopterin converting factor subunit 2

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MPRKYARNIGSFTLAGQKKLLASKVMVVGLGGLGGYVLEELCRAGVGEIVGVDGDAFEESNLNRQLLATEKNLGQKKANK
ARKRAAEINQTVVVSGFVTKVENLPETAWQGVELVFDCLDNIESRFYLEEKTNNLGLPLVHGAIGGWYGQVGIVWPGSNL
LTKIYRERKKGIEQTLGNPPFTPAVAASLMVALGINLLLGTLEKESVFYFFDLKNMEFEKISF

Sequences:

>Translated_223_residues
MPRKYARNIGSFTLAGQKKLLASKVMVVGLGGLGGYVLEELCRAGVGEIVGVDGDAFEESNLNRQLLATEKNLGQKKANK
ARKRAAEINQTVVVSGFVTKVENLPETAWQGVELVFDCLDNIESRFYLEEKTNNLGLPLVHGAIGGWYGQVGIVWPGSNL
LTKIYRERKKGIEQTLGNPPFTPAVAASLMVALGINLLLGTLEKESVFYFFDLKNMEFEKISF
>Mature_222_residues
PRKYARNIGSFTLAGQKKLLASKVMVVGLGGLGGYVLEELCRAGVGEIVGVDGDAFEESNLNRQLLATEKNLGQKKANKA
RKRAAEINQTVVVSGFVTKVENLPETAWQGVELVFDCLDNIESRFYLEEKTNNLGLPLVHGAIGGWYGQVGIVWPGSNLL
TKIYRERKKGIEQTLGNPPFTPAVAASLMVALGINLLLGTLEKESVFYFFDLKNMEFEKISF

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=155, Percent_Identity=32.9032258064516, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI87082356, Length=157, Percent_Identity=31.8471337579618, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787048, Length=172, Percent_Identity=31.9767441860465, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789177, Length=148, Percent_Identity=33.1081081081081, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=159, Percent_Identity=32.0754716981132, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193203301, Length=163, Percent_Identity=28.8343558282209, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320598, Length=181, Percent_Identity=30.939226519337, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321903, Length=142, Percent_Identity=35.2112676056338, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322639, Length=185, Percent_Identity=28.6486486486486, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325323, Length=146, Percent_Identity=30.1369863013699, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582879, Length=155, Percent_Identity=34.8387096774194, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24660640, Length=181, Percent_Identity=30.939226519337, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 24472; Mature: 24340

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKYARNIGSFTLAGQKKLLASKVMVVGLGGLGGYVLEELCRAGVGEIVGVDGDAFEES
CCHHHHHCCCCEEECCHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCEEECCCCCCCCC
NLNRQLLATEKNLGQKKANKARKRAAEINQTVVVSGFVTKVENLPETAWQGVELVFDCLD
CCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHH
NIESRFYLEEKTNNLGLPLVHGAIGGWYGQVGIVWPGSNLLTKIYRERKKGIEQTLGNPP
HHHHHEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
FTPAVAASLMVALGINLLLGTLEKESVFYFFDLKNMEFEKISF
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCC
>Mature Secondary Structure 
PRKYARNIGSFTLAGQKKLLASKVMVVGLGGLGGYVLEELCRAGVGEIVGVDGDAFEES
CHHHHHCCCCEEECCHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCEEECCCCCCCCC
NLNRQLLATEKNLGQKKANKARKRAAEINQTVVVSGFVTKVENLPETAWQGVELVFDCLD
CCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHH
NIESRFYLEEKTNNLGLPLVHGAIGGWYGQVGIVWPGSNLLTKIYRERKKGIEQTLGNPP
HHHHHEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
FTPAVAASLMVALGINLLLGTLEKESVFYFFDLKNMEFEKISF
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]