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The map label for this gene is cobB [H]

Identifier: 78043052

GI number: 78043052

Start: 219050

End: 219766

Strand: Direct

Name: cobB [H]

Synonym: CHY_0257

Alternate gene names: 78043052

Gene position: 219050-219766 (Clockwise)

Preceding gene: 78042633

Following gene: 78043646

Centisome position: 9.12

GC content: 50.07

Gene sequence:

>717_bases
GTGGAAGAAGCGGTTAAGATGCTACTTACGGCCCGTCACGCTATTGCCTTTACCGGAGCGGGAGTTAGCACCGAAAGCGG
GATTCCGGATTTTCGTGGAAATTCTGGATTGTGGGAACAGTATCCGGTGGAGAAAGTGGCCTCAAGAAGAGCCCTTATGG
AGAATCCGGCGTTTTTTTTAAATTTTTACCGGGAGCGGTTTAAAAGCTATGCCAATGTTAAGCCCAATCGGGCCCATGAA
GCCCTGGCGCGGATGGAAAAAGCGGGAATAATAAAAGGCATTGTGACCCAAAACATTGATGGTTTGCACCAGAAAGCAGG
CAGTAAAAACGTTATAGAAATCCATGGCACCTTAAAACGGGTACGGTGCGACCGGTGCGGAAAATATTACTTGCCGGAGA
AGTTAGACGAAGAAGAAGTACCCCGGTGTAATTGCGGCGGTGTTATCCGTCCCGATGTAGTTTTATTTGGGGAAGCTTTA
CCCCGGCGGGAGTGGCAAATAGCCCTGGAACTGGCTGAGCGTTCGGATCTTGTCCTGGTGGTGGGGTCGTCTCTGGTAGT
TACGCCGGCCAATCAAATTCCCGGGCTGGTTCTCTTAGAAGGAGGAAAGGCAATAATTGTCAATAAAGACCCGACGCCCC
TGGATGACCAGGCGCTGGTTCTTAGAGGATATGCCGGGGAAATTCTTTCCAAGCTGGCGGATATGTTAGGTGTTTAA

Upstream 100 bases:

>100_bases
TAGCGTAAAAGCCGAAAAACAATTTCCCGGTCCGTCATCCTTTTCACCTCCAATATTGGTTCTAATTTATCATATGAAAA
TTATTAACGGAGGGGATTTT

Downstream 100 bases:

>100_bases
GCAGGCCCGAAGGAACCCTTCGGGTTTTATAATATAGTTTGGGGTGTTTTGTATGGAACGTTTTTTGTTAAAAGCAAATT
ATACGCCCAAGGGAGATCAA

Product: Sir2 family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MEEAVKMLLTARHAIAFTGAGVSTESGIPDFRGNSGLWEQYPVEKVASRRALMENPAFFLNFYRERFKSYANVKPNRAHE
ALARMEKAGIIKGIVTQNIDGLHQKAGSKNVIEIHGTLKRVRCDRCGKYYLPEKLDEEEVPRCNCGGVIRPDVVLFGEAL
PRREWQIALELAERSDLVLVVGSSLVVTPANQIPGLVLLEGGKAIIVNKDPTPLDDQALVLRGYAGEILSKLADMLGV

Sequences:

>Translated_238_residues
MEEAVKMLLTARHAIAFTGAGVSTESGIPDFRGNSGLWEQYPVEKVASRRALMENPAFFLNFYRERFKSYANVKPNRAHE
ALARMEKAGIIKGIVTQNIDGLHQKAGSKNVIEIHGTLKRVRCDRCGKYYLPEKLDEEEVPRCNCGGVIRPDVVLFGEAL
PRREWQIALELAERSDLVLVVGSSLVVTPANQIPGLVLLEGGKAIIVNKDPTPLDDQALVLRGYAGEILSKLADMLGV
>Mature_238_residues
MEEAVKMLLTARHAIAFTGAGVSTESGIPDFRGNSGLWEQYPVEKVASRRALMENPAFFLNFYRERFKSYANVKPNRAHE
ALARMEKAGIIKGIVTQNIDGLHQKAGSKNVIEIHGTLKRVRCDRCGKYYLPEKLDEEEVPRCNCGGVIRPDVVLFGEAL
PRREWQIALELAERSDLVLVVGSSLVVTPANQIPGLVLLEGGKAIIVNKDPTPLDDQALVLRGYAGEILSKLADMLGV

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912664, Length=246, Percent_Identity=36.9918699186992, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI13787215, Length=246, Percent_Identity=36.9918699186992, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI300795542, Length=228, Percent_Identity=38.5964912280702, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI300797577, Length=253, Percent_Identity=29.6442687747036, Blast_Score=117, Evalue=6e-27,
Organism=Homo sapiens, GI6912662, Length=267, Percent_Identity=31.0861423220974, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7657575, Length=260, Percent_Identity=31.5384615384615, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI63054862, Length=183, Percent_Identity=37.7049180327869, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI6912660, Length=202, Percent_Identity=36.1386138613861, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI300797597, Length=243, Percent_Identity=28.8065843621399, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI13775602, Length=217, Percent_Identity=30.8755760368664, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI13775600, Length=196, Percent_Identity=32.6530612244898, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI300797705, Length=192, Percent_Identity=32.2916666666667, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI7706712, Length=261, Percent_Identity=28.735632183908, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI215982798, Length=199, Percent_Identity=30.6532663316583, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI308199517, Length=229, Percent_Identity=35.3711790393013, Blast_Score=122, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17541892, Length=250, Percent_Identity=34.8, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17567771, Length=264, Percent_Identity=31.0606060606061, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71990482, Length=285, Percent_Identity=27.0175438596491, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990487, Length=287, Percent_Identity=26.8292682926829, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17505510, Length=246, Percent_Identity=28.8617886178862, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325242, Length=263, Percent_Identity=31.5589353612167, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324599, Length=276, Percent_Identity=26.8115942028986, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320397, Length=235, Percent_Identity=27.2340425531915, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24645650, Length=255, Percent_Identity=28.2352941176471, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648389, Length=215, Percent_Identity=31.6279069767442, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28571445, Length=199, Percent_Identity=32.6633165829146, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137536, Length=234, Percent_Identity=27.3504273504274, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28571443, Length=132, Percent_Identity=34.0909090909091, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571441, Length=132, Percent_Identity=34.0909090909091, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650933, Length=266, Percent_Identity=26.6917293233083, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26224; Mature: 26224

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEAVKMLLTARHAIAFTGAGVSTESGIPDFRGNSGLWEQYPVEKVASRRALMENPAFFL
CHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
NFYRERFKSYANVKPNRAHEALARMEKAGIIKGIVTQNIDGLHQKAGSKNVIEIHGTLKR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECHHHHH
VRCDRCGKYYLPEKLDEEEVPRCNCGGVIRPDVVLFGEALPRREWQIALELAERSDLVLV
HHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHEEECCCCCEEEE
VGSSLVVTPANQIPGLVLLEGGKAIIVNKDPTPLDDQALVLRGYAGEILSKLADMLGV
ECCCEEEECHHHCCCEEEEECCCEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEEAVKMLLTARHAIAFTGAGVSTESGIPDFRGNSGLWEQYPVEKVASRRALMENPAFFL
CHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
NFYRERFKSYANVKPNRAHEALARMEKAGIIKGIVTQNIDGLHQKAGSKNVIEIHGTLKR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECHHHHH
VRCDRCGKYYLPEKLDEEEVPRCNCGGVIRPDVVLFGEALPRREWQIALELAERSDLVLV
HHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHEEECCCCCEEEE
VGSSLVVTPANQIPGLVLLEGGKAIIVNKDPTPLDDQALVLRGYAGEILSKLADMLGV
ECCCEEEECHHHCCCEEEEECCCEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]