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The map label for this gene is rpfG [H]

Identifier: 78042857

GI number: 78042857

Start: 1810781

End: 1812433

Strand: Direct

Name: rpfG [H]

Synonym: CHY_2028

Alternate gene names: 78042857

Gene position: 1810781-1812433 (Clockwise)

Preceding gene: 78043410

Following gene: 78043265

Centisome position: 75.4

GC content: 37.27

Gene sequence:

>1653_bases
ATGTTAAATAATCCTATACCTGCCTATATTAGAGTTAACAATCTTAAAAACCCTCTGCAAAAGGTAACCGAATTTCATCA
GCTACTGGCAAAATTTAAAGACCCCGATGACATCTTAAGGGAAGTATGTTTTTACCTTAAAGATTTATGGCAGGCCTTAG
GATGTAATATTGTCATCCCCAAAAACCCCAAATATGAATTTGAACTAAAGTCCCATTATTCATCTGCCGCTAAACCACTT
CCGGAAAACATCAATGAAAACCAGGGGCTCAGTATTTATGTTTTTAAGGAAAAACACCCTGTTTTAATAAACGATACCAC
CACTTCTCCCCTTACCAACAAAGAAATAGTTAACTACTACGGTCACAAAAGTGCTATTGCAGCTCCTATGGTCGGAAAGT
CTAAAACTTTGGGTGTAATTATAGTATTTCATAAAGAAAAAGAGTTTTTTACCATTGAAGACTTAGAACTTTTGACATTT
TTTGCAAATCATACGGCTTACGTCATAGAAAATCTACAGCTCTTATACCGGCTGGAAAAAGGTATATATCAAGATTTCTT
AACCGGCGTTTATAATTACCGTTACCTGCAGGAATGCCTTTTAAAAATAGCCGAAGACCAAACAAAGCTTCCACTTTCTG
TTATCATGATCGACCTGGACAATTTTAAAAAGTTTAATGACATGTTTGGCCACAGAGCCGGTGACCAAATCATTAAAACC
ACCGCAAAAATCATAAAAAGTTCCGTACGCAAAAACGACCTTGTTTTTCGCTACGGTGGTGATGAGTTTGCCGTTTTACT
TCCAGCAACTGACTACCAAACTGCTTTAAACGTAGCCCAAAGAATATCAGAAAAAATCAAACAATATACTTATGTTATTG
AGAAAAATCGTTTTCGCGGTTTTATTAGAGCCTCGGTAGGAGCTTCAACTTTAGGGATTGCTCCGACCCCGCAAATGGTT
TTGGATCTTGCCGATAAAGCCATGTACCAGATTAAAAGAAGCCGCTTTAACATTTTAAACCACATTGCTATAGAAGAAGA
AATACTTTCCGATTTTAACCAGGCGGAAAAAACCTTATTTAAAACGTTAAAAGTTTTATTATCCTTAATTGACTTTAAAG
ACCACTATACTTTAGAACATTCCCGTCAGGTAAGCATCTTGTCTTACCATGTGGCCAAAAACTTAAACCTTGACCAGGAA
AGCCTCAAAATTATTTATTTAGCAGGACTTTTACACGATGTGGGTAAAATCTGGATTGATGAAGCAATTATCAATAAACC
GGGACCTCTAAACCTTTTAGAATCAGGTGCAATTAAAAAACATCCAGTTATCGGCGCCGAAATATTAAAGCCCATATCTT
TTTTAAAACCTTTGATTCCAATAATTTACCACCACCATGAAAAATACGATGGCAGCGGTTATCCCGACGGTCTTTCGGGA
AAAAATATCCCTTTACTTGCGCGGATAATTACTTTGGCCGATAGCTTTGACGCCATGACCTCAACCCGCCCTTACCGTGA
ACCTTTAAATTTCAAAGAAGCCATCCTTGAAATAAAAAAATGCAGTGGCAGCCACTTTGACCCCGACTTGCTGGATGTCT
TTATAAAAACCTTATGCCAGCTAAAAAGCGAAACTACGGAGATTTTTAGCTAA

Upstream 100 bases:

>100_bases
AAAAACCAATGTATACATTTTCACAATTTTTGCAAATAAAAAAGGATTTTACCATCTTATGTAGAAATACTCAATATAAA
GTACAAGCGGGAGGATAATT

Downstream 100 bases:

>100_bases
TCGGTTTTTAAATACCCCTTTTTTGACGTATCCAAAACCAAGAGCTACACCTTCATCGCAGATTAAATACCAGCCTTCCG
ACCACGGTACCGAAAAGCTT

Product: GGDEF/GAF/HDIG domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MLNNPIPAYIRVNNLKNPLQKVTEFHQLLAKFKDPDDILREVCFYLKDLWQALGCNIVIPKNPKYEFELKSHYSSAAKPL
PENINENQGLSIYVFKEKHPVLINDTTTSPLTNKEIVNYYGHKSAIAAPMVGKSKTLGVIIVFHKEKEFFTIEDLELLTF
FANHTAYVIENLQLLYRLEKGIYQDFLTGVYNYRYLQECLLKIAEDQTKLPLSVIMIDLDNFKKFNDMFGHRAGDQIIKT
TAKIIKSSVRKNDLVFRYGGDEFAVLLPATDYQTALNVAQRISEKIKQYTYVIEKNRFRGFIRASVGASTLGIAPTPQMV
LDLADKAMYQIKRSRFNILNHIAIEEEILSDFNQAEKTLFKTLKVLLSLIDFKDHYTLEHSRQVSILSYHVAKNLNLDQE
SLKIIYLAGLLHDVGKIWIDEAIINKPGPLNLLESGAIKKHPVIGAEILKPISFLKPLIPIIYHHHEKYDGSGYPDGLSG
KNIPLLARIITLADSFDAMTSTRPYREPLNFKEAILEIKKCSGSHFDPDLLDVFIKTLCQLKSETTEIFS

Sequences:

>Translated_550_residues
MLNNPIPAYIRVNNLKNPLQKVTEFHQLLAKFKDPDDILREVCFYLKDLWQALGCNIVIPKNPKYEFELKSHYSSAAKPL
PENINENQGLSIYVFKEKHPVLINDTTTSPLTNKEIVNYYGHKSAIAAPMVGKSKTLGVIIVFHKEKEFFTIEDLELLTF
FANHTAYVIENLQLLYRLEKGIYQDFLTGVYNYRYLQECLLKIAEDQTKLPLSVIMIDLDNFKKFNDMFGHRAGDQIIKT
TAKIIKSSVRKNDLVFRYGGDEFAVLLPATDYQTALNVAQRISEKIKQYTYVIEKNRFRGFIRASVGASTLGIAPTPQMV
LDLADKAMYQIKRSRFNILNHIAIEEEILSDFNQAEKTLFKTLKVLLSLIDFKDHYTLEHSRQVSILSYHVAKNLNLDQE
SLKIIYLAGLLHDVGKIWIDEAIINKPGPLNLLESGAIKKHPVIGAEILKPISFLKPLIPIIYHHHEKYDGSGYPDGLSG
KNIPLLARIITLADSFDAMTSTRPYREPLNFKEAILEIKKCSGSHFDPDLLDVFIKTLCQLKSETTEIFS
>Mature_550_residues
MLNNPIPAYIRVNNLKNPLQKVTEFHQLLAKFKDPDDILREVCFYLKDLWQALGCNIVIPKNPKYEFELKSHYSSAAKPL
PENINENQGLSIYVFKEKHPVLINDTTTSPLTNKEIVNYYGHKSAIAAPMVGKSKTLGVIIVFHKEKEFFTIEDLELLTF
FANHTAYVIENLQLLYRLEKGIYQDFLTGVYNYRYLQECLLKIAEDQTKLPLSVIMIDLDNFKKFNDMFGHRAGDQIIKT
TAKIIKSSVRKNDLVFRYGGDEFAVLLPATDYQTALNVAQRISEKIKQYTYVIEKNRFRGFIRASVGASTLGIAPTPQMV
LDLADKAMYQIKRSRFNILNHIAIEEEILSDFNQAEKTLFKTLKVLLSLIDFKDHYTLEHSRQVSILSYHVAKNLNLDQE
SLKIIYLAGLLHDVGKIWIDEAIINKPGPLNLLESGAIKKHPVIGAEILKPISFLKPLIPIIYHHHEKYDGSGYPDGLSG
KNIPLLARIITLADSFDAMTSTRPYREPLNFKEAILEIKKCSGSHFDPDLLDVFIKTLCQLKSETTEIFS

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Integral Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=194, Percent_Identity=35.5670103092784, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=35.8490566037736, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=34.9112426035503, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081881, Length=122, Percent_Identity=39.344262295082, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=31.9526627218935, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=201, Percent_Identity=29.3532338308458, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788381, Length=142, Percent_Identity=32.3943661971831, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787802, Length=168, Percent_Identity=30.3571428571429, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87081974, Length=132, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788085, Length=178, Percent_Identity=26.4044943820225, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787541, Length=155, Percent_Identity=31.6129032258064, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=208, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 62845; Mature: 62845

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNPIPAYIRVNNLKNPLQKVTEFHQLLAKFKDPDDILREVCFYLKDLWQALGCNIVIP
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEC
KNPKYEFELKSHYSSAAKPLPENINENQGLSIYVFKEKHPVLINDTTTSPLTNKEIVNYY
CCCCEEEEEHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHH
GHKSAIAAPMVGKSKTLGVIIVFHKEKEFFTIEDLELLTFFANHTAYVIENLQLLYRLEK
CCCHHHCCCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GIYQDFLTGVYNYRYLQECLLKIAEDQTKLPLSVIMIDLDNFKKFNDMFGHRAGDQIIKT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHH
TAKIIKSSVRKNDLVFRYGGDEFAVLLPATDYQTALNVAQRISEKIKQYTYVIEKNRFRG
HHHHHHHHHCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHE
FIRASVGASTLGIAPTPQMVLDLADKAMYQIKRSRFNILNHIAIEEEILSDFNQAEKTLF
HHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLKVLLSLIDFKDHYTLEHSRQVSILSYHVAKNLNLDQESLKIIYLAGLLHDVGKIWID
HHHHHHHHHHHCCCCCEECCCCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAIINKPGPLNLLESGAIKKHPVIGAEILKPISFLKPLIPIIYHHHEKYDGSGYPDGLSG
HHHHCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KNIPLLARIITLADSFDAMTSTRPYREPLNFKEAILEIKKCSGSHFDPDLLDVFIKTLCQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LKSETTEIFS
HHHHHHHHCC
>Mature Secondary Structure
MLNNPIPAYIRVNNLKNPLQKVTEFHQLLAKFKDPDDILREVCFYLKDLWQALGCNIVIP
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEC
KNPKYEFELKSHYSSAAKPLPENINENQGLSIYVFKEKHPVLINDTTTSPLTNKEIVNYY
CCCCEEEEEHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHH
GHKSAIAAPMVGKSKTLGVIIVFHKEKEFFTIEDLELLTFFANHTAYVIENLQLLYRLEK
CCCHHHCCCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GIYQDFLTGVYNYRYLQECLLKIAEDQTKLPLSVIMIDLDNFKKFNDMFGHRAGDQIIKT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHH
TAKIIKSSVRKNDLVFRYGGDEFAVLLPATDYQTALNVAQRISEKIKQYTYVIEKNRFRG
HHHHHHHHHCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHE
FIRASVGASTLGIAPTPQMVLDLADKAMYQIKRSRFNILNHIAIEEEILSDFNQAEKTLF
HHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLKVLLSLIDFKDHYTLEHSRQVSILSYHVAKNLNLDQESLKIIYLAGLLHDVGKIWID
HHHHHHHHHHHCCCCCEECCCCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAIINKPGPLNLLESGAIKKHPVIGAEILKPISFLKPLIPIIYHHHEKYDGSGYPDGLSG
HHHHCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KNIPLLARIITLADSFDAMTSTRPYREPLNFKEAILEIKKCSGSHFDPDLLDVFIKTLCQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LKSETTEIFS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]