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The map label for this gene is thiM

Identifier: 78042771

GI number: 78042771

Start: 659332

End: 660135

Strand: Direct

Name: thiM

Synonym: CHY_0748

Alternate gene names: 78042771

Gene position: 659332-660135 (Clockwise)

Preceding gene: 78043716

Following gene: 78043238

Centisome position: 27.45

GC content: 44.03

Gene sequence:

>804_bases
ATGCTTAACACCCTATGGGAAATCAGGGAGAAGGTCCGGGACTTATCTCCTCTGGTGGTAAATTTAACCAATAATGTGGT
GACTAATTTTACCGCAAATGTACTTTTAGCGGCCGGGGCTTCGCCGATCATGTCGGAAGGGGTAGAAGAAGCTGACGATT
TAGTGAAAATTGCTAATGCAGTGGTCATAAATATCGGAACCCTTCACTCCCGTCAGGTTGAGTACTTTAAGAAAGCAGTT
TATTTGGCCAATAAATATCAAAAGCCTTTGCTGTTAGACCCGGTGGGTTTGGGAGCAACCACATACCGCAACGAAACGAC
CTTTGAACTTTTAAATTCTGGAAATTTCACATTAATCAGGGGTAATTACGGTGAAATAAGCTTTTTGGCGGGAAATTCTG
CCCAGGTTAAAGGGGTGGACAGCCAAACGTCCGATTTTGCAGCCGAAAATCTTACCGAAGTGGCTAAACGCTACAAAACT
GTTGTGGTGGCAACTGGGCCGGTAGATTACGTTATTGCTGAAGGAGAATTGTATTTAAACCGTACCGGGGATATAATGCT
TCAAAAAGTGACCGGAACCGGGTGTGCTCTAACCTCATTGATTGGTGCTTTTGTTGGGGTAATTGATGAACCGGCTCTTG
CGGCCTTAGCGGCTTTAGCTTTTTACGGAGCGGCTTCCGAAAAAGCCCGGAAAATAAGTGCCGGTCCCGGGAGTTTTCTG
GTAAATTTTATAGATTCTCTTTATAATTTAACTAAAGAAGAGTTTTTGGAGCTAACTACGGAAAAAGTTCAAGGGCTACG
GTGA

Upstream 100 bases:

>100_bases
AGGAAAATGCGAATAGAGCGCGGCTCTCAAGACATAAAGAGCTGTTCCATGTGCGGGGAACTTTGTGCCATGAAAATTAT
GAATGAAAGGGGCGGAAAAA

Downstream 100 bases:

>100_bases
GAAAATGGAAGTTACAAAGATTGGGGAAGAAGGGGTTATAGCTATTGCCAAGAAAGCTACTGAAGTATCCCCCGGCGTTA
TTTTGGGAATCGGGGATGAT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLNTLWEIREKVRDLSPLVVNLTNNVVTNFTANVLLAAGASPIMSEGVEEADDLVKIANAVVINIGTLHSRQVEYFKKAV
YLANKYQKPLLLDPVGLGATTYRNETTFELLNSGNFTLIRGNYGEISFLAGNSAQVKGVDSQTSDFAAENLTEVAKRYKT
VVVATGPVDYVIAEGELYLNRTGDIMLQKVTGTGCALTSLIGAFVGVIDEPALAALAALAFYGAASEKARKISAGPGSFL
VNFIDSLYNLTKEEFLELTTEKVQGLR

Sequences:

>Translated_267_residues
MLNTLWEIREKVRDLSPLVVNLTNNVVTNFTANVLLAAGASPIMSEGVEEADDLVKIANAVVINIGTLHSRQVEYFKKAV
YLANKYQKPLLLDPVGLGATTYRNETTFELLNSGNFTLIRGNYGEISFLAGNSAQVKGVDSQTSDFAAENLTEVAKRYKT
VVVATGPVDYVIAEGELYLNRTGDIMLQKVTGTGCALTSLIGAFVGVIDEPALAALAALAFYGAASEKARKISAGPGSFL
VNFIDSLYNLTKEEFLELTTEKVQGLR
>Mature_267_residues
MLNTLWEIREKVRDLSPLVVNLTNNVVTNFTANVLLAAGASPIMSEGVEEADDLVKIANAVVINIGTLHSRQVEYFKKAV
YLANKYQKPLLLDPVGLGATTYRNETTFELLNSGNFTLIRGNYGEISFLAGNSAQVKGVDSQTSDFAAENLTEVAKRYKT
VVVATGPVDYVIAEGELYLNRTGDIMLQKVTGTGCALTSLIGAFVGVIDEPALAALAALAFYGAASEKARKISAGPGSFL
VNFIDSLYNLTKEEFLELTTEKVQGLR

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=239, Percent_Identity=40.1673640167364, Blast_Score=156, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325042, Length=279, Percent_Identity=35.1254480286738, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_CARHZ (Q3AE32)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359602.1
- ProteinModelPortal:   Q3AE32
- SMR:   Q3AE32
- STRING:   Q3AE32
- GeneID:   3726904
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0748
- NMPDR:   fig|246194.3.peg.781
- TIGR:   CHY_0748
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   CLSK717776
- BioCyc:   CHYD246194:CHY_0748-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28780; Mature: 28780

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 120-120 BINDING 165-165 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTLWEIREKVRDLSPLVVNLTNNVVTNFTANVLLAAGASPIMSEGVEEADDLVKIANA
CCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHEECCCCHHHHHCHHHHHHHHHHHHH
VVINIGTLHSRQVEYFKKAVYLANKYQKPLLLDPVGLGATTYRNETTFELLNSGNFTLIR
HEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEECCCCCHHHHCCCCCEEEEE
GNYGEISFLAGNSAQVKGVDSQTSDFAAENLTEVAKRYKTVVVATGPVDYVIAEGELYLN
CCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCEEEEEECCEEEEE
RTGDIMLQKVTGTGCALTSLIGAFVGVIDEPALAALAALAFYGAASEKARKISAGPGSFL
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHH
VNFIDSLYNLTKEEFLELTTEKVQGLR
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNTLWEIREKVRDLSPLVVNLTNNVVTNFTANVLLAAGASPIMSEGVEEADDLVKIANA
CCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHEECCCCHHHHHCHHHHHHHHHHHHH
VVINIGTLHSRQVEYFKKAVYLANKYQKPLLLDPVGLGATTYRNETTFELLNSGNFTLIR
HEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEECCCCCHHHHCCCCCEEEEE
GNYGEISFLAGNSAQVKGVDSQTSDFAAENLTEVAKRYKTVVVATGPVDYVIAEGELYLN
CCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCEEEEEECCEEEEE
RTGDIMLQKVTGTGCALTSLIGAFVGVIDEPALAALAALAFYGAASEKARKISAGPGSFL
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHH
VNFIDSLYNLTKEEFLELTTEKVQGLR
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA