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The map label for this gene is proA

Identifier: 78042736

GI number: 78042736

Start: 328296

End: 329543

Strand: Direct

Name: proA

Synonym: CHY_0383

Alternate gene names: 78042736

Gene position: 328296-329543 (Clockwise)

Preceding gene: 78044812

Following gene: 78044322

Centisome position: 13.67

GC content: 48.72

Gene sequence:

>1248_bases
ATGGATGAAGTGTTAAATTTAGCTAAGAAAGCCAAAGAGGCTTCGAAAAAACTTGCTCAGCTAAGTACCGAACAAAAAAA
CCGGGCGCTTTTAAAGATAGCCCAATACCTGGAAGAGAATATGGAGAAAATTTTAACCGAAAACCAAAAGGATCTGGCGG
AAGCGAAAAAAGGCGGCCTATCGCCGGCTTTTATTGAAAGGCTTACGTTAAACGAGAAAAGGATTTTGGATATGGCGGAA
GGGGTAAGGCAGGTAGCCAAACTTCCCGACCCCGTTGGTGAAGTTCTCGGAATGACCCGCCGACCCAATGGCCTTGTAAT
TGGTCAGGTGCGGGTTCCCCTGGGGGTGGTAGGGATTATCTATGAATCCCGGCCAAATGTAACCGTAGATGCAGCGGCTT
TGTGCCTTAAAGCAGGAAATGCGGTTATCCTTCGGGGTGGGAAGGAAGCTTTTAACTCAAACCTTGCTTTAGTTACTCTT
ATGGAAGAAGCGCTCCGGAGCGAGGAAATTCCCGAAGGGGCAGTAGGGATGATTAAAACCACCAGCCGGGATGCGGCTAA
TTATTTAATGCGGCTAAACGGTTATTTAGACGTTTTAATCCCCCGGGGTGGTGCCGGTTTAATTAAAACGGTGGTGGAAA
ACAGTACAGTTCCGGTAATTGAAACGGGAGTAGGTAACTGCCACGTTTACGTGGAAGAAGACGCGGACCTGGAAATGGCT
GAAAGGATAATCATTAATGCCAAATGCCAGCGGCCGGCGGTATGTAACGCCATGGAAACCCTGCTGGTGCACGAAAAAAT
TGCTCCGGTTTTCCTACCCCAAATTGGGAAGGCCTTAAAAGAAAATGGTGTGGAAATTAGGGGGTGTGAGGTAACCCGCC
GGTATATTCCCGACGCCTTACCGGCAACGGAAGAAGATTACTACACGGAATTTTTAGACCTTATCTTGGCGGTGCGGGTG
GTTCGGGATTTAGATGAGGCAATTGCCCATATTACCAAGTACGGTTCGGGTCACTCGGAAGCAATTGTTACCCGGGATTA
CTTTAAAGCGAGAAGGTTTACCGAGGAAGTGGATGCGGCGGCGGTTTATGTCAATGCTTCTACCCGCTTTACCGATGGCT
TTGAATTTGGTTTTGGAGCGGAGATTGGGATTTCCACCCAGAAACTGCACGCTCGGGGGCCAATGGGCTTAAAAGAGTTA
ACCACCACCAAGTACGTTATCTACGGCACCGGCCAGATCCGGGGCTAA

Upstream 100 bases:

>100_bases
GGGGGCAAATCAAATATCCGAAAGGAGGCGAAAACCTGGTCTGGGGGCCGACTTGGTTGAAAATAAAAAACAGTCAAGTT
TTTTGAACCAGGATGGTTAG

Downstream 100 bases:

>100_bases
ACTCCGTTCCCATATTTACGTCCCATACCGCAAACTAAGGGACGGTTCTTTTTTTTTTAGGCGTATTGACGCAAATATAA
TAAGGTGTTTGACAGATGTT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MDEVLNLAKKAKEASKKLAQLSTEQKNRALLKIAQYLEENMEKILTENQKDLAEAKKGGLSPAFIERLTLNEKRILDMAE
GVRQVAKLPDPVGEVLGMTRRPNGLVIGQVRVPLGVVGIIYESRPNVTVDAAALCLKAGNAVILRGGKEAFNSNLALVTL
MEEALRSEEIPEGAVGMIKTTSRDAANYLMRLNGYLDVLIPRGGAGLIKTVVENSTVPVIETGVGNCHVYVEEDADLEMA
ERIIINAKCQRPAVCNAMETLLVHEKIAPVFLPQIGKALKENGVEIRGCEVTRRYIPDALPATEEDYYTEFLDLILAVRV
VRDLDEAIAHITKYGSGHSEAIVTRDYFKARRFTEEVDAAAVYVNASTRFTDGFEFGFGAEIGISTQKLHARGPMGLKEL
TTTKYVIYGTGQIRG

Sequences:

>Translated_415_residues
MDEVLNLAKKAKEASKKLAQLSTEQKNRALLKIAQYLEENMEKILTENQKDLAEAKKGGLSPAFIERLTLNEKRILDMAE
GVRQVAKLPDPVGEVLGMTRRPNGLVIGQVRVPLGVVGIIYESRPNVTVDAAALCLKAGNAVILRGGKEAFNSNLALVTL
MEEALRSEEIPEGAVGMIKTTSRDAANYLMRLNGYLDVLIPRGGAGLIKTVVENSTVPVIETGVGNCHVYVEEDADLEMA
ERIIINAKCQRPAVCNAMETLLVHEKIAPVFLPQIGKALKENGVEIRGCEVTRRYIPDALPATEEDYYTEFLDLILAVRV
VRDLDEAIAHITKYGSGHSEAIVTRDYFKARRFTEEVDAAAVYVNASTRFTDGFEFGFGAEIGISTQKLHARGPMGLKEL
TTTKYVIYGTGQIRG
>Mature_415_residues
MDEVLNLAKKAKEASKKLAQLSTEQKNRALLKIAQYLEENMEKILTENQKDLAEAKKGGLSPAFIERLTLNEKRILDMAE
GVRQVAKLPDPVGEVLGMTRRPNGLVIGQVRVPLGVVGIIYESRPNVTVDAAALCLKAGNAVILRGGKEAFNSNLALVTL
MEEALRSEEIPEGAVGMIKTTSRDAANYLMRLNGYLDVLIPRGGAGLIKTVVENSTVPVIETGVGNCHVYVEEDADLEMA
ERIIINAKCQRPAVCNAMETLLVHEKIAPVFLPQIGKALKENGVEIRGCEVTRRYIPDALPATEEDYYTEFLDLILAVRV
VRDLDEAIAHITKYGSGHSEAIVTRDYFKARRFTEEVDAAAVYVNASTRFTDGFEFGFGAEIGISTQKLHARGPMGLKEL
TTTKYVIYGTGQIRG

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=407, Percent_Identity=39.8034398034398, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI62912457, Length=407, Percent_Identity=39.8034398034398, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=52.2058823529412, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17569967, Length=411, Percent_Identity=37.4695863746959, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17569969, Length=411, Percent_Identity=37.4695863746959, Blast_Score=282, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6324899, Length=434, Percent_Identity=38.0184331797235, Blast_Score=285, Evalue=1e-77,
Organism=Drosophila melanogaster, GI21357643, Length=406, Percent_Identity=40.8866995073892, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_CARHZ (Q3AF39)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359245.1
- ProteinModelPortal:   Q3AF39
- SMR:   Q3AF39
- STRING:   Q3AF39
- GeneID:   3726908
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0383
- NMPDR:   fig|246194.3.peg.599
- TIGR:   CHY_0383
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   CHYD246194:CHY_0383-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45490; Mature: 45490

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEVLNLAKKAKEASKKLAQLSTEQKNRALLKIAQYLEENMEKILTENQKDLAEAKKGGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
SPAFIERLTLNEKRILDMAEGVRQVAKLPDPVGEVLGMTRRPNGLVIGQVRVPLGVVGII
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEECCEEEEEEE
YESRPNVTVDAAALCLKAGNAVILRGGKEAFNSNLALVTLMEEALRSEEIPEGAVGMIKT
EECCCCEEHHHHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
TSRDAANYLMRLNGYLDVLIPRGGAGLIKTVVENSTVPVIETGVGNCHVYVEEDADLEMA
CCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCHHHH
ERIIINAKCQRPAVCNAMETLLVHEKIAPVFLPQIGKALKENGVEIRGCEVTRRYIPDAL
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCC
PATEEDYYTEFLDLILAVRVVRDLDEAIAHITKYGSGHSEAIVTRDYFKARRFTEEVDAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCE
AVYVNASTRFTDGFEFGFGAEIGISTQKLHARGPMGLKELTTTKYVIYGTGQIRG
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCEEEEEEECCEECC
>Mature Secondary Structure
MDEVLNLAKKAKEASKKLAQLSTEQKNRALLKIAQYLEENMEKILTENQKDLAEAKKGGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
SPAFIERLTLNEKRILDMAEGVRQVAKLPDPVGEVLGMTRRPNGLVIGQVRVPLGVVGII
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEECCEEEEEEE
YESRPNVTVDAAALCLKAGNAVILRGGKEAFNSNLALVTLMEEALRSEEIPEGAVGMIKT
EECCCCEEHHHHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
TSRDAANYLMRLNGYLDVLIPRGGAGLIKTVVENSTVPVIETGVGNCHVYVEEDADLEMA
CCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCHHHH
ERIIINAKCQRPAVCNAMETLLVHEKIAPVFLPQIGKALKENGVEIRGCEVTRRYIPDAL
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCC
PATEEDYYTEFLDLILAVRVVRDLDEAIAHITKYGSGHSEAIVTRDYFKARRFTEEVDAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCE
AVYVNASTRFTDGFEFGFGAEIGISTQKLHARGPMGLKELTTTKYVIYGTGQIRG
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCEEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA