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The map label for this gene is yvoF [H]

Identifier: 78042659

GI number: 78042659

Start: 243332

End: 243820

Strand: Direct

Name: yvoF [H]

Synonym: CHY_0277

Alternate gene names: 78042659

Gene position: 243332-243820 (Clockwise)

Preceding gene: 78044467

Following gene: 78045229

Centisome position: 10.13

GC content: 45.19

Gene sequence:

>489_bases
ATGAGGAGGTTAAAGGAATATCCCAAGCCGGGACCGGAGAACTCGATGTACTACTGGCGCAGTTATGTAAACTTTTTTAA
AGTGGCTAAAAATTTTATATTTATCAGCATAGGCAGGTATTTGCCATTTCTCGGATTAAAGCGGTGGCTTTACCGGTACG
GTGTTGGGATGAAAATTGGTAAAAATGTGAGCCTTGGCTTAATGATGATGCCCGATGTGCTTTTTCCCGAATTAATCGAA
ATTGGGGACAACACCATTATTGGTTACAATGCTACGATTTTAACCCACGAGTTTCGTGTGGAGAGCTTTAAAACCGGGCC
AGTTAAAATTGGCCGGGATGTTTTAATTGGTGCAAACGCTACAATTTTGGCGGGAGTGGAAATTGGGGATGGAGCAATGA
TTGGAGCGGGAGCGGTGGTGACGAAAGATATTCCTCCCGGGGTGTTAGCGGTGGGAGTGCCCGCCCGGGTGGTGAAAAAA
ATTGAGTAA

Upstream 100 bases:

>100_bases
ATTTGCGGGAGAAGATGGCCCGGTATGTAAGGGAGAAATCCGGAATTAAAACTATTGCCGGTAAGGATGGTTTAAAACTT
GAACTGAATCCGGAGGGAGT

Downstream 100 bases:

>100_bases
AATTACCGGGGTAATCGCTGACTACTGGCAAAACCCCCGCAAAAAGCTTTATGTTTTTTTGCTGCTGCTGGTAAATTTAT
TTGGCAGTATCTGGGGGTAC

Product: hexapeptide transferase family protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MRRLKEYPKPGPENSMYYWRSYVNFFKVAKNFIFISIGRYLPFLGLKRWLYRYGVGMKIGKNVSLGLMMMPDVLFPELIE
IGDNTIIGYNATILTHEFRVESFKTGPVKIGRDVLIGANATILAGVEIGDGAMIGAGAVVTKDIPPGVLAVGVPARVVKK
IE

Sequences:

>Translated_162_residues
MRRLKEYPKPGPENSMYYWRSYVNFFKVAKNFIFISIGRYLPFLGLKRWLYRYGVGMKIGKNVSLGLMMMPDVLFPELIE
IGDNTIIGYNATILTHEFRVESFKTGPVKIGRDVLIGANATILAGVEIGDGAMIGAGAVVTKDIPPGVLAVGVPARVVKK
IE
>Mature_162_residues
MRRLKEYPKPGPENSMYYWRSYVNFFKVAKNFIFISIGRYLPFLGLKRWLYRYGVGMKIGKNVSLGLMMMPDVLFPELIE
IGDNTIIGYNATILTHEFRVESFKTGPVKIGRDVLIGANATILAGVEIGDGAMIGAGAVVTKDIPPGVLAVGVPARVVKK
IE

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=120, Percent_Identity=40, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1786664, Length=117, Percent_Identity=38.4615384615385, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=116, Percent_Identity=38.7931034482759, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 17956; Mature: 17956

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLKEYPKPGPENSMYYWRSYVNFFKVAKNFIFISIGRYLPFLGLKRWLYRYGVGMKIG
CCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHCCCEEEC
KNVSLGLMMMPDVLFPELIEIGDNTIIGYNATILTHEFRVESFKTGPVKIGRDVLIGANA
CCCCEEEEECCHHHHHHHHHCCCCEEEECCEEEEEEEEEECCCCCCCEEECCEEEEECCC
TILAGVEIGDGAMIGAGAVVTKDIPPGVLAVGVPARVVKKIE
EEEEEEEECCCCEECCCEEEECCCCCCEEEECCCHHHHHHCC
>Mature Secondary Structure
MRRLKEYPKPGPENSMYYWRSYVNFFKVAKNFIFISIGRYLPFLGLKRWLYRYGVGMKIG
CCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHCCCEEEC
KNVSLGLMMMPDVLFPELIEIGDNTIIGYNATILTHEFRVESFKTGPVKIGRDVLIGANA
CCCCEEEEECCHHHHHHHHHCCCCEEEECCEEEEEEEEEECCCCCCCEEECCEEEEECCC
TILAGVEIGDGAMIGAGAVVTKDIPPGVLAVGVPARVVKKIE
EEEEEEEECCCCEECCCEEEECCCCCCEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]