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The map label for this gene is selU [H]

Identifier: 77465606

GI number: 77465606

Start: 699059

End: 700111

Strand: Direct

Name: selU [H]

Synonym: RSP_3604

Alternate gene names: 77465606

Gene position: 699059-700111 (Clockwise)

Preceding gene: 77465605

Following gene: 77465607

Centisome position: 74.13

GC content: 71.7

Gene sequence:

>1053_bases
ATGGCCTATCGTGCAACCGACCTTTCGCAGCTCATCGAGCACGGGTTCGACGATGTGATCGACGTGCGCTCGCCTGCCGA
ATGGCAGGAGGACCGCGTGCCCGGAGCGATCAACCTGCCCGTCCTCGACAATGAGGAGCGCGCCCGGGTCGGAACGATCT
ACAAGCAGGTCAACCCCTTCTCGGCCCGCAAGATCGGCGCGGCGCTGGTGGCCCGCAATGCCGCGCGTCACATCGAGGAG
GTTCTGGCCGACCGTCCGGGCGGCTGGCGCCCGCTCGTCTACTGCTGGCGCGGCGGGCAGAGGTCGAACTCCTTCGCCAT
GATCCTCGGCCAGATCGGCTGGCGGGTCGAGGTGCTGGAAGGCGGCTACAAGAGCTGGCGCGGCCTCGTGGTGCAGGAGC
TCTACGACCGGCCGGTCCGGGCGCCCGTGGTGGTGATCGACGGAAACACCGGGGCCGCGAAGACCGAGATCCTGACGCAT
CTGGCCGCCTTCGGCGTGCAGGTCCTCGATCTCGAGGGGCTCGCCAATCACCGCGGCTCGCTCTTCGGCGCCTTGGCGGG
CGGTCAGCCCGCGCAGCGCGGCTTCGAAAGCCGCCTCGCCGTGGCGCTGGCCCGGCTCGATCCGACGCAGCCGGTGGTGG
TCGAGGCGGAATCGAGCAAGATCGGCGAGATCCGGCTGCCGCCGGGCCTCTGGAAGGCGATGCAGGCCGCGCCGCGGATC
GAGGTCGCCGCCCCCGTCGCGGCCCGCGCGGCCTATCTCGTGCGCGCCTATCGCGATCTGGTCGAGGACGGCCCGCGGCT
CGCCGCCGTCATCGACCTTCTGCGCCCCGTCCATCCGGCAAAACGCATCGAGGAATGGCACGCGCACGCCGCCGCGGGCC
GGTTCGAGGAGCTCGCGGCCGATCTGATGCAGCGGCATTACGATCCGCGCTACGCCAAGCACCGCGCCCGCTCGGCCCCG
CCGCGCGCGGCGGTCGAGACCGACCGGCTGGACGATCTGCCGGCCCTTGCGGCCCGGATCGCCGCGCAGGTCGCGCGGCT
GGCGGAGGGCTGA

Upstream 100 bases:

>100_bases
ATCTTACCGCGTAGGAAAGAGACCACGCCTGCGACGCTGCCGTCCCGTCGCGAGTGGCAGGGCTGCTGGCAAAGGGGCGC
GGGGCGGGCTAGGAAGGCGC

Downstream 100 bases:

>100_bases
ATGGAGCGCGGCGCGCGCGATCTGGTGCTGATCGGCGGCGGGCACGCCCATGCGCTGGTGCTGCTCGGCTGGAAGCCGGT
GCCAGACCTGCGCGTCACCC

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAYRATDLSQLIEHGFDDVIDVRSPAEWQEDRVPGAINLPVLDNEERARVGTIYKQVNPFSARKIGAALVARNAARHIEE
VLADRPGGWRPLVYCWRGGQRSNSFAMILGQIGWRVEVLEGGYKSWRGLVVQELYDRPVRAPVVVIDGNTGAAKTEILTH
LAAFGVQVLDLEGLANHRGSLFGALAGGQPAQRGFESRLAVALARLDPTQPVVVEAESSKIGEIRLPPGLWKAMQAAPRI
EVAAPVAARAAYLVRAYRDLVEDGPRLAAVIDLLRPVHPAKRIEEWHAHAAAGRFEELAADLMQRHYDPRYAKHRARSAP
PRAAVETDRLDDLPALAARIAAQVARLAEG

Sequences:

>Translated_350_residues
MAYRATDLSQLIEHGFDDVIDVRSPAEWQEDRVPGAINLPVLDNEERARVGTIYKQVNPFSARKIGAALVARNAARHIEE
VLADRPGGWRPLVYCWRGGQRSNSFAMILGQIGWRVEVLEGGYKSWRGLVVQELYDRPVRAPVVVIDGNTGAAKTEILTH
LAAFGVQVLDLEGLANHRGSLFGALAGGQPAQRGFESRLAVALARLDPTQPVVVEAESSKIGEIRLPPGLWKAMQAAPRI
EVAAPVAARAAYLVRAYRDLVEDGPRLAAVIDLLRPVHPAKRIEEWHAHAAAGRFEELAADLMQRHYDPRYAKHRARSAP
PRAAVETDRLDDLPALAARIAAQVARLAEG
>Mature_349_residues
AYRATDLSQLIEHGFDDVIDVRSPAEWQEDRVPGAINLPVLDNEERARVGTIYKQVNPFSARKIGAALVARNAARHIEEV
LADRPGGWRPLVYCWRGGQRSNSFAMILGQIGWRVEVLEGGYKSWRGLVVQELYDRPVRAPVVVIDGNTGAAKTEILTHL
AAFGVQVLDLEGLANHRGSLFGALAGGQPAQRGFESRLAVALARLDPTQPVVVEAESSKIGEIRLPPGLWKAMQAAPRIE
VAAPVAARAAYLVRAYRDLVEDGPRLAAVIDLLRPVHPAKRIEEWHAHAAAGRFEELAADLMQRHYDPRYAKHRARSAPP
RAAVETDRLDDLPALAARIAAQVARLAEG

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=259, Percent_Identity=36.6795366795367, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 38362; Mature: 38231

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYRATDLSQLIEHGFDDVIDVRSPAEWQEDRVPGAINLPVLDNEERARVGTIYKQVNPF
CCCCHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCEEECCEECCCCHHHHHHHHHHCCCH
SARKIGAALVARNAARHIEEVLADRPGGWRPLVYCWRGGQRSNSFAMILGQIGWRVEVLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHEEECCCCEEEEEC
GGYKSWRGLVVQELYDRPVRAPVVVIDGNTGAAKTEILTHLAAFGVQVLDLEGLANHRGS
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCEEEEEHHHHHHCCCC
LFGALAGGQPAQRGFESRLAVALARLDPTQPVVVEAESSKIGEIRLPPGLWKAMQAAPRI
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECCHHHHHHHHHCCCC
EVAAPVAARAAYLVRAYRDLVEDGPRLAAVIDLLRPVHPAKRIEEWHAHAAAGRFEELAA
EECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHH
DLMQRHYDPRYAKHRARSAPPRAAVETDRLDDLPALAARIAAQVARLAEG
HHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYRATDLSQLIEHGFDDVIDVRSPAEWQEDRVPGAINLPVLDNEERARVGTIYKQVNPF
CCCHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCEEECCEECCCCHHHHHHHHHHCCCH
SARKIGAALVARNAARHIEEVLADRPGGWRPLVYCWRGGQRSNSFAMILGQIGWRVEVLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHEEECCCCEEEEEC
GGYKSWRGLVVQELYDRPVRAPVVVIDGNTGAAKTEILTHLAAFGVQVLDLEGLANHRGS
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCEEEEEHHHHHHCCCC
LFGALAGGQPAQRGFESRLAVALARLDPTQPVVVEAESSKIGEIRLPPGLWKAMQAAPRI
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECCHHHHHHHHHCCCC
EVAAPVAARAAYLVRAYRDLVEDGPRLAAVIDLLRPVHPAKRIEEWHAHAAAGRFEELAA
EECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHH
DLMQRHYDPRYAKHRARSAPPRAAVETDRLDDLPALAARIAAQVARLAEG
HHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]