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The map label for this gene is lsrK [H]

Identifier: 77465507

GI number: 77465507

Start: 584793

End: 586322

Strand: Direct

Name: lsrK [H]

Synonym: RSP_3506

Alternate gene names: 77465507

Gene position: 584793-586322 (Clockwise)

Preceding gene: 77465506

Following gene: 77465508

Centisome position: 62.01

GC content: 72.16

Gene sequence:

>1530_bases
ATGCCCTATTTCCTCGCCATCGACGCCGGAACCGGCAGCGGCCGCGCCGTCCTCTTCGACGAGACCGGCGCCGAGATCGC
GGCCTCGGGTCAGGAATGGTGGCATGTGGCCGACCCGCGCTACCCCGGCTCTATGGATTTCGACACCGAAGGCAACTGGG
CCATTCTCGCCCGCTGCTGTCGCGAGGTGATCGCGCGGAGCGGCGTCGATCCCGCAGAGATCCGCGCGGTCAGCGCGACC
GCCATGCGCGAGGCCTTCGTGCTGCATGACGCGACAGGCCGCGAGATCTGGGCCTGCGCCAATGTCGATGCCCGCGCGGG
CGCCGAGGTGCGCGAGCTGAAGGAAGACCATCCGGGGCTTGAGGCTCAGATCTATGCGCGCTCGGGCCAGACCTACGCGC
TCGGCGCGCTGCCGCGGCTGGCCTGGCTCCGCCGCCACGCACCCGAGACGCTGGCGCGGGCGCGCAGCCTCACCATGCTG
TCCGACTGGATCCTCTACCGGCTCTCGGGCGAGCTGACGCTGGAGCCGTCGAACGGCGGCACCACCGGCCTTCTCGATCT
GCGCACCCGCGCGCCCCTGACCGACGCGCTGGCCACCGTGGGGCTGCCCACAGACCTCTTCCCGCGTGGCGTCACCACCG
GCGAGGTGGTGGGCGAGGTGCAGACGGCGGCCGCCGCCGAGACCGGCCTCGCCCCCGGCACGCTGGTCGTGGCGGGCGGC
GGCGATTGCCAGACGGGCGCGCTTGGCTTGGGCATCGTGCGCGAGGGCGACTGCGCCGTGCTCGGCGGCACCTTCTGGCA
GCAGGTGGTCAATGTTCCGCTGACGCTCACCGATCCGACCATGAACCTGCGGATCAACCCGCACATGGTGGCGGGCCTGA
ATCAGGCCGAGGCCATCAGCTTCTTCGTGGGCGCCGTGATGCGCTGGTTCCGCGACAGCTTCGGACAGGGCCGCGACTAT
ACCGCCCTCGAGGCGGATTCGATGCAGGTCCCTCCGGGGGCGCACGGGATCATTCCGATCTTCTCCGACGTGATGCGCTA
CGGCGACTGGTTCCACGCCGCGCCGTCGCTGCTGAACCTCTCGCTCGATCCGGCGGCCTCGGGGCCTGCCGCGATCTTCC
GCGCCCTGCAGGAGAATGCGGCCATCGTCGCGCGGCGCAACCTCGAGAGCGTCTTCGCCCTCACCGGCCACCGGCCCGAG
CGGCTGGTCTTCGCGGGCGGCGCCGCAAAATCGGCGCACTGGTCGCAGATCCTGGCCGACGTCACCGGGCTCACCGTCGT
CACGCCGGAGGTCAAGGAGGCCACCGCGCAGGGCTGCGCCTCGGCCGCGGCCACCGGTGCGGGCCATTTCGCCTCGCTCG
CCGAGGCGGGCGGTGCCTGGATGCGGATCGACCGCACCTTCACGCCGGATCCCGCGCTCCGCCCGCTCTACGACGAGGCC
GGCGACCGCTGGGCCCGCGCCTATGCCGCGCAGCGCGGCCTCGTGCAGGACGGCACCGCCACCGCCATGTGGCGCGCCCC
CGGCACCTGA

Upstream 100 bases:

>100_bases
CGGCCCCATCCTCTCGGCCCTGCGCGGCGAGATCCTCGACATTCTCATCACCGACGAGCCCACGGCGCTCGCCCTTCTCG
ACGCGGCGGAGGCCTGACCC

Downstream 100 bases:

>100_bases
CGATTCTCCCTTCCAGAAAGGACTAGGCCATGCTGATCCAACTCGTGAACATCAAGGTGCAGGACGGCACCCGCGAGACC
TTCCTCGAAGCCTTCCGGCT

Product: autoinducer-2 (AI-2) kinase

Products: NA

Alternate protein names: AI-2 kinase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPYFLAIDAGTGSGRAVLFDETGAEIAASGQEWWHVADPRYPGSMDFDTEGNWAILARCCREVIARSGVDPAEIRAVSAT
AMREAFVLHDATGREIWACANVDARAGAEVRELKEDHPGLEAQIYARSGQTYALGALPRLAWLRRHAPETLARARSLTML
SDWILYRLSGELTLEPSNGGTTGLLDLRTRAPLTDALATVGLPTDLFPRGVTTGEVVGEVQTAAAAETGLAPGTLVVAGG
GDCQTGALGLGIVREGDCAVLGGTFWQQVVNVPLTLTDPTMNLRINPHMVAGLNQAEAISFFVGAVMRWFRDSFGQGRDY
TALEADSMQVPPGAHGIIPIFSDVMRYGDWFHAAPSLLNLSLDPAASGPAAIFRALQENAAIVARRNLESVFALTGHRPE
RLVFAGGAAKSAHWSQILADVTGLTVVTPEVKEATAQGCASAAATGAGHFASLAEAGGAWMRIDRTFTPDPALRPLYDEA
GDRWARAYAAQRGLVQDGTATAMWRAPGT

Sequences:

>Translated_509_residues
MPYFLAIDAGTGSGRAVLFDETGAEIAASGQEWWHVADPRYPGSMDFDTEGNWAILARCCREVIARSGVDPAEIRAVSAT
AMREAFVLHDATGREIWACANVDARAGAEVRELKEDHPGLEAQIYARSGQTYALGALPRLAWLRRHAPETLARARSLTML
SDWILYRLSGELTLEPSNGGTTGLLDLRTRAPLTDALATVGLPTDLFPRGVTTGEVVGEVQTAAAAETGLAPGTLVVAGG
GDCQTGALGLGIVREGDCAVLGGTFWQQVVNVPLTLTDPTMNLRINPHMVAGLNQAEAISFFVGAVMRWFRDSFGQGRDY
TALEADSMQVPPGAHGIIPIFSDVMRYGDWFHAAPSLLNLSLDPAASGPAAIFRALQENAAIVARRNLESVFALTGHRPE
RLVFAGGAAKSAHWSQILADVTGLTVVTPEVKEATAQGCASAAATGAGHFASLAEAGGAWMRIDRTFTPDPALRPLYDEA
GDRWARAYAAQRGLVQDGTATAMWRAPGT
>Mature_508_residues
PYFLAIDAGTGSGRAVLFDETGAEIAASGQEWWHVADPRYPGSMDFDTEGNWAILARCCREVIARSGVDPAEIRAVSATA
MREAFVLHDATGREIWACANVDARAGAEVRELKEDHPGLEAQIYARSGQTYALGALPRLAWLRRHAPETLARARSLTMLS
DWILYRLSGELTLEPSNGGTTGLLDLRTRAPLTDALATVGLPTDLFPRGVTTGEVVGEVQTAAAAETGLAPGTLVVAGGG
DCQTGALGLGIVREGDCAVLGGTFWQQVVNVPLTLTDPTMNLRINPHMVAGLNQAEAISFFVGAVMRWFRDSFGQGRDYT
ALEADSMQVPPGAHGIIPIFSDVMRYGDWFHAAPSLLNLSLDPAASGPAAIFRALQENAAIVARRNLESVFALTGHRPER
LVFAGGAAKSAHWSQILADVTGLTVVTPEVKEATAQGCASAAATGAGHFASLAEAGGAWMRIDRTFTPDPALRPLYDEAG
DRWARAYAAQRGLVQDGTATAMWRAPGT

Specific function: Catalyzes the phosphorylation of autoinducer 2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of 4,5-dihydroxy-2,3- pentanedione

COG id: COG1070

COG function: function code G; Sugar (pentulose and hexulose) kinases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=464, Percent_Identity=22.6293103448276, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787789, Length=517, Percent_Identity=52.2243713733075, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789168, Length=487, Percent_Identity=25.2566735112936, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790005, Length=507, Percent_Identity=27.6134122287968, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790361, Length=487, Percent_Identity=27.5154004106776, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789138, Length=384, Percent_Identity=22.65625, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535599, Length=462, Percent_Identity=23.3766233766234, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018484 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: NA

Molecular weight: Translated: 54005; Mature: 53874

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYFLAIDAGTGSGRAVLFDETGAEIAASGQEWWHVADPRYPGSMDFDTEGNWAILARCC
CCEEEEEECCCCCCCEEEEECCCCCEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHH
REVIARSGVDPAEIRAVSATAMREAFVLHDATGREIWACANVDARAGAEVRELKEDHPGL
HHHHHHCCCCHHHHEEHHHHHHHHHHEEECCCCCEEEEECCCCHHCCCHHHHHHHHCCCC
EAQIYARSGQTYALGALPRLAWLRRHAPETLARARSLTMLSDWILYRLSGELTLEPSNGG
EEEEEEECCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCEEEECCCCC
TTGLLDLRTRAPLTDALATVGLPTDLFPRGVTTGEVVGEVQTAAAAETGLAPGTLVVAGG
CEEEEEECCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECC
GDCQTGALGLGIVREGDCAVLGGTFWQQVVNVPLTLTDPTMNLRINPHMVAGLNQAEAIS
CCCCCCCEEEEEEECCCEEEECHHHHHHHHCCCEEEECCCEEEEECCCEEECCCHHHHHH
FFVGAVMRWFRDSFGQGRDYTALEADSMQVPPGAHGIIPIFSDVMRYGDWFHAAPSLLNL
HHHHHHHHHHHHHCCCCCCCEEECCCCEECCCCCCCCHHHHHHHHHHCCHHHCCCHHEEE
SLDPAASGPAAIFRALQENAAIVARRNLESVFALTGHRPERLVFAGGAAKSAHWSQILAD
ECCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHH
VTGLTVVTPEVKEATAQGCASAAATGAGHFASLAEAGGAWMRIDRTFTPDPALRPLYDEA
HCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHH
GDRWARAYAAQRGLVQDGTATAMWRAPGT
HHHHHHHHHHHCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
PYFLAIDAGTGSGRAVLFDETGAEIAASGQEWWHVADPRYPGSMDFDTEGNWAILARCC
CEEEEEECCCCCCCEEEEECCCCCEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHH
REVIARSGVDPAEIRAVSATAMREAFVLHDATGREIWACANVDARAGAEVRELKEDHPGL
HHHHHHCCCCHHHHEEHHHHHHHHHHEEECCCCCEEEEECCCCHHCCCHHHHHHHHCCCC
EAQIYARSGQTYALGALPRLAWLRRHAPETLARARSLTMLSDWILYRLSGELTLEPSNGG
EEEEEEECCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCEEEECCCCC
TTGLLDLRTRAPLTDALATVGLPTDLFPRGVTTGEVVGEVQTAAAAETGLAPGTLVVAGG
CEEEEEECCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECC
GDCQTGALGLGIVREGDCAVLGGTFWQQVVNVPLTLTDPTMNLRINPHMVAGLNQAEAIS
CCCCCCCEEEEEEECCCEEEECHHHHHHHHCCCEEEECCCEEEEECCCEEECCCHHHHHH
FFVGAVMRWFRDSFGQGRDYTALEADSMQVPPGAHGIIPIFSDVMRYGDWFHAAPSLLNL
HHHHHHHHHHHHHCCCCCCCEEECCCCEECCCCCCCCHHHHHHHHHHCCHHHCCCHHEEE
SLDPAASGPAAIFRALQENAAIVARRNLESVFALTGHRPERLVFAGGAAKSAHWSQILAD
ECCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHH
VTGLTVVTPEVKEATAQGCASAAATGAGHFASLAEAGGAWMRIDRTFTPDPALRPLYDEA
HCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHH
GDRWARAYAAQRGLVQDGTATAMWRAPGT
HHHHHHHHHHHCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA