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The map label for this gene is ilvG [H]

Identifier: 77465169

GI number: 77465169

Start: 203970

End: 205622

Strand: Reverse

Name: ilvG [H]

Synonym: RSP_3155

Alternate gene names: 77465169

Gene position: 205622-203970 (Counterclockwise)

Preceding gene: 77465172

Following gene: 77465168

Centisome position: 21.8

GC content: 73.56

Gene sequence:

>1653_bases
ATGAACGACCGCCGCCCCTTCCTGCTTCGCGGAGCGGATCTTCTCGTCTCCACGCTGGTCGATTGCGGGGTGACCCGGAT
CTTCAGCCTGTCGGGCAACCAGATCATGCCGCTCTACGACGCCTGTCTGGGCTCCGGGATCGAGATCGTCCATGTGCGTC
ACGAGGCGGCCGCCGTTTTCATGGCCGAGGGCTGGGCGCAGCTTACGGGCGAAGTGGGCGTGGCGCTGGTCACGGCGGGC
CCGGGGGCTGCCAATGCGGTGGGGCCGCTGATGAGCGCGCGCCAGTCCGAGACGCCGCTGCTGCTCCTTAGCGGGGACTC
GCCCCGGGCGCAGGACGGGATGGGGGCGTTCCAGACCCTCGATCAGGTGGCCGCGACCGCGCCCTTCACCAAACATTCCG
CGCGCGTGGGCTCGGTGCCGACGCTGGCGCACGAGCTCAGGGCGGCGCTGACGCTGGCCCGGGCGGGGCGGCCGGGGCCG
GTGCATCTGGCGCTGCCGCAGGATCTTCTGACCCAGCGCGCCGAGGGTGTGCCGAGCAGCAGCCGGACCGCAGCCGCCAC
GGTGCCGGCCAGCGAGCGCGAGACCGCGAGGCTCGCGGCCGAGATCGCGGAAGCGCGCCGCCCGCTGATCGTGACGAGCG
GCCTCTTCAGCCCGACCCGCGGCGGCGATCTGGCCCGCAGGCTCGGTCAGAGGCTCGCCGTTCCGGTCGTGGCGCTGGAG
AGCCCGCGCGGCCTGCGCGATCCGGCCCAGCCCGGCCTGCGGCAGCGGATGGCCGAGGCGGATCTCGTGATCTCGCTCGG
CAAGTCCGTCGATTACATGCTCGACTTCGGTCGGGCCACCTCCGCGGACTGCGGCTGGATCGTGGTCGAGCCCGAGGACG
GGGCGGGCGAAGAGGCCGTCCGCAACCTCGGCCCGAAGCTGCGCCGCCTGATCGCGGCTGATCCGCGGGCGCTGGCGCAG
GGGCTGGCGGACCTGCCCGAGGCCGGCTGCGACGCGGCGCGCCGCGACTGGTGCGCCCGCTTCGGACGGCGGCCCGCGCC
GCCCGCACCGGATGAGACGCCGGGGCCGATCGATTCCGGGCTGCTCTGCGCCACCCTGTCCGACGTTCTGGGCGCGGACG
GGGGCGAGACGATCCTCGTCTCGGACGGCGGCGAGTTCGGCCAGTGGGCGCAGGCCCTCGTGCAGGCCGACCGACGGCTC
ATCAACGGGCCCGCAGGCGGGATCGGCGGGGCGCTCGGGCATGCGGTGGCGGCAAGCCTCGCCTGCCCCGAGGCCCGTAT
CGCGGTCGCCAGCGGGGATGGCAGCATCGGCTTTCATCTGGCAGAACTCGAGACGGCGGTGCGCGCGGGCGCGGCCTTCG
TGGTGGTGATCGGCAACGACCGCCGCTGGAATGCCGAGCATCTGCTGCAGCTTCGCGAATTCGGCCCCGACCGCGTCCAT
GGATGCGAGCTTTCGGGCGCCCGTTACGATCTGGTCGCCGCGGCCCTCGGGGGCACGGGCGCCCATGTGACATGCCGGTC
CGAGCTTCGGGGCGCCCTGCGGCGCGCCTTCGCCGCCGGCGGAGTGGTTCTGGTGAATGTGGAGATCGAGGGCCGCGCAG
CCCCTTCCGGAGAGGAGCCGGAAGCGGCGGAGACGGCGCAGCCCGACGGATGA

Upstream 100 bases:

>100_bases
GTCAATGGGAACAGGTGGAGCCTCGCCGAACCGCTCCGCCCCCTTCGTTCGAGACCAGATCGCGGCCGATGGCCGTGCAC
CAGCCGGAGGCATGATCCCG

Downstream 100 bases:

>100_bases
TGGCCAGGGGTGCGCGCTTCGGCGCGCCGGCCGAGCAAACGGGAGGAAACATGACACACAGCGAGCCCTTGCTGTCGGTC
CGGGGGGTCACGATCCAGTA

Product: thiamine pyrophosphate-requiring enzyme

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MNDRRPFLLRGADLLVSTLVDCGVTRIFSLSGNQIMPLYDACLGSGIEIVHVRHEAAAVFMAEGWAQLTGEVGVALVTAG
PGAANAVGPLMSARQSETPLLLLSGDSPRAQDGMGAFQTLDQVAATAPFTKHSARVGSVPTLAHELRAALTLARAGRPGP
VHLALPQDLLTQRAEGVPSSSRTAAATVPASERETARLAAEIAEARRPLIVTSGLFSPTRGGDLARRLGQRLAVPVVALE
SPRGLRDPAQPGLRQRMAEADLVISLGKSVDYMLDFGRATSADCGWIVVEPEDGAGEEAVRNLGPKLRRLIAADPRALAQ
GLADLPEAGCDAARRDWCARFGRRPAPPAPDETPGPIDSGLLCATLSDVLGADGGETILVSDGGEFGQWAQALVQADRRL
INGPAGGIGGALGHAVAASLACPEARIAVASGDGSIGFHLAELETAVRAGAAFVVVIGNDRRWNAEHLLQLREFGPDRVH
GCELSGARYDLVAAALGGTGAHVTCRSELRGALRRAFAAGGVVLVNVEIEGRAAPSGEEPEAAETAQPDG

Sequences:

>Translated_550_residues
MNDRRPFLLRGADLLVSTLVDCGVTRIFSLSGNQIMPLYDACLGSGIEIVHVRHEAAAVFMAEGWAQLTGEVGVALVTAG
PGAANAVGPLMSARQSETPLLLLSGDSPRAQDGMGAFQTLDQVAATAPFTKHSARVGSVPTLAHELRAALTLARAGRPGP
VHLALPQDLLTQRAEGVPSSSRTAAATVPASERETARLAAEIAEARRPLIVTSGLFSPTRGGDLARRLGQRLAVPVVALE
SPRGLRDPAQPGLRQRMAEADLVISLGKSVDYMLDFGRATSADCGWIVVEPEDGAGEEAVRNLGPKLRRLIAADPRALAQ
GLADLPEAGCDAARRDWCARFGRRPAPPAPDETPGPIDSGLLCATLSDVLGADGGETILVSDGGEFGQWAQALVQADRRL
INGPAGGIGGALGHAVAASLACPEARIAVASGDGSIGFHLAELETAVRAGAAFVVVIGNDRRWNAEHLLQLREFGPDRVH
GCELSGARYDLVAAALGGTGAHVTCRSELRGALRRAFAAGGVVLVNVEIEGRAAPSGEEPEAAETAQPDG
>Mature_550_residues
MNDRRPFLLRGADLLVSTLVDCGVTRIFSLSGNQIMPLYDACLGSGIEIVHVRHEAAAVFMAEGWAQLTGEVGVALVTAG
PGAANAVGPLMSARQSETPLLLLSGDSPRAQDGMGAFQTLDQVAATAPFTKHSARVGSVPTLAHELRAALTLARAGRPGP
VHLALPQDLLTQRAEGVPSSSRTAAATVPASERETARLAAEIAEARRPLIVTSGLFSPTRGGDLARRLGQRLAVPVVALE
SPRGLRDPAQPGLRQRMAEADLVISLGKSVDYMLDFGRATSADCGWIVVEPEDGAGEEAVRNLGPKLRRLIAADPRALAQ
GLADLPEAGCDAARRDWCARFGRRPAPPAPDETPGPIDSGLLCATLSDVLGADGGETILVSDGGEFGQWAQALVQADRRL
INGPAGGIGGALGHAVAASLACPEARIAVASGDGSIGFHLAELETAVRAGAAFVVVIGNDRRWNAEHLLQLREFGPDRVH
GCELSGARYDLVAAALGGTGAHVTCRSELRGALRRAFAAGGVVLVNVEIEGRAAPSGEEPEAAETAQPDG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=543, Percent_Identity=30.5709023941068, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=25.177304964539, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=29.4014084507042, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=27.8388278388278, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=24.2805755395683, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=25.5639097744361, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787096, Length=535, Percent_Identity=24.1121495327103, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17542570, Length=578, Percent_Identity=28.0276816608997, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=25.044404973357, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=25.044404973357, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=27.3851590106007, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=530, Percent_Identity=23.0188679245283, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922626, Length=543, Percent_Identity=24.6777163904236, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56945; Mature: 56945

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRRPFLLRGADLLVSTLVDCGVTRIFSLSGNQIMPLYDACLGSGIEIVHVRHEAAAVF
CCCCCCEEEECHHHHHHHHHHHCHHEEEECCCCEECHHHHHHHCCCEEEEEEEHHHHEEH
MAEGWAQLTGEVGVALVTAGPGAANAVGPLMSARQSETPLLLLSGDSPRAQDGMGAFQTL
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHH
DQVAATAPFTKHSARVGSVPTLAHELRAALTLARAGRPGPVHLALPQDLLTQRAEGVPSS
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCC
SRTAAATVPASERETARLAAEIAEARRPLIVTSGLFSPTRGGDLARRLGQRLAVPVVALE
CCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEC
SPRGLRDPAQPGLRQRMAEADLVISLGKSVDYMLDFGRATSADCGWIVVEPEDGAGEEAV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCCHHHH
RNLGPKLRRLIAADPRALAQGLADLPEAGCDAARRDWCARFGRRPAPPAPDETPGPIDSG
HHHCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LLCATLSDVLGADGGETILVSDGGEFGQWAQALVQADRRLINGPAGGIGGALGHAVAASL
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ACPEARIAVASGDGSIGFHLAELETAVRAGAAFVVVIGNDRRWNAEHLLQLREFGPDRVH
CCCCCEEEEECCCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCC
GCELSGARYDLVAAALGGTGAHVTCRSELRGALRRAFAAGGVVLVNVEIEGRAAPSGEEP
CCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
EAAETAQPDG
CCCCCCCCCC
>Mature Secondary Structure
MNDRRPFLLRGADLLVSTLVDCGVTRIFSLSGNQIMPLYDACLGSGIEIVHVRHEAAAVF
CCCCCCEEEECHHHHHHHHHHHCHHEEEECCCCEECHHHHHHHCCCEEEEEEEHHHHEEH
MAEGWAQLTGEVGVALVTAGPGAANAVGPLMSARQSETPLLLLSGDSPRAQDGMGAFQTL
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHH
DQVAATAPFTKHSARVGSVPTLAHELRAALTLARAGRPGPVHLALPQDLLTQRAEGVPSS
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCC
SRTAAATVPASERETARLAAEIAEARRPLIVTSGLFSPTRGGDLARRLGQRLAVPVVALE
CCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEC
SPRGLRDPAQPGLRQRMAEADLVISLGKSVDYMLDFGRATSADCGWIVVEPEDGAGEEAV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEECCCCCCHHHH
RNLGPKLRRLIAADPRALAQGLADLPEAGCDAARRDWCARFGRRPAPPAPDETPGPIDSG
HHHCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LLCATLSDVLGADGGETILVSDGGEFGQWAQALVQADRRLINGPAGGIGGALGHAVAASL
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ACPEARIAVASGDGSIGFHLAELETAVRAGAAFVVVIGNDRRWNAEHLLQLREFGPDRVH
CCCCCEEEEECCCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCC
GCELSGARYDLVAAALGGTGAHVTCRSELRGALRRAFAAGGVVLVNVEIEGRAAPSGEEP
CCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
EAAETAQPDG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]