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The map label for this gene is pknA [H]

Identifier: 77465142

GI number: 77465142

Start: 173637

End: 174758

Strand: Direct

Name: pknA [H]

Synonym: RSP_3129

Alternate gene names: 77465142

Gene position: 173637-174758 (Clockwise)

Preceding gene: 77465140

Following gene: 77465143

Centisome position: 18.41

GC content: 71.48

Gene sequence:

>1122_bases
ATGACCGAGCCGCGCCTTCCCAACGGCACGCGCATCGCCGGCCATGTCATCACGGACCAGATCGGCCGGGGCGGGTTCGG
GATCACCTACCGGGCGAACGTGCTCGAGACCGGCGCCACCGTCGCGCTGAAGGAATACTTCCCCTGCGACATGGCCCATC
GCGATGCGCAGGGCTTCGTCCGCCCGCTGCCCAGGGCCGAGCGGATGTACGAGGAGGGTCTGCGGGCCTTCATCACCGAG
GCGAACATCCTGAAGGCGCTGCCGCAGCAGGCCGGTCTGGTGCGGGTGCGCGGCGCCTTCGAGAAGTTCGGCACCGCCTA
TTGCGCGATGGATTTCATCGAGGGCGAGCCGCTCAGCCGTCTCGCGCAGGCCACGCTCCAGCGCACCGGCTTCATCGCCG
AGGATCTGCTGCGCAGCTTCCTCGCCCCGATCTGCCTCGCGCTCGAGACGGTTCATTCGACGAACCTCGTCCACCGCGAT
GTGAAGCCCGCCAACATCATGATCTCGCGCCGCGACCAGCAGCCGATCCTGATCGACTTCGGCGCCGCGCGCCCCTCGGG
GCAGCGCGGGTCGCTCGCGATGTTCACCCGCAGCTACGCCCCGGTCGAGCTGTTCCCCCGCGCGCAGGTGCGTTCGGCGC
AGCCCTTGGAGGAGGGGCCGTGGAGCGACATCTATTCGCTGTCCGTCATGCTCTACGAGATGGCGACGCGGCAGCTGCCC
CCCAGCGCGCCCGACCGGGCGGCGGCGGTCGCCGCGGGGGCGCCCGACCCTTACGTCCCGCTGACGGCGCTTCTTGCGCA
GCGGCGGCTGCGCGGCAGCTACTCGCCCGCCTTCGCCTCGGCGGTCGATGCCGGCTGCGCGCTCTGGCCCGAGGCGCGGC
CGCGGACGGTACGCCAGTTCGCCTACATGGCCGGCGTCGAGATGGGGTCCGCTCCGCCGCCGACGCCGCGCCGAACCGTC
ACCGGGGCCGCTCCCTCGCCCTCCTCGTCGGTTCCGCGCAGCCAGCGACGCCCCGTGAACCGCCAGTCCATCGCCATGGT
GCTCCTGATGCTGCTGATCGCGGGGGCGGCGGTGTCCTATGGCGTCGTCTCGCGCCAGCCCGACTGGGTGGCCTACCGAT
GA

Upstream 100 bases:

>100_bases
CTGCTGCACCGGAGGCCAGCCCGGCACCTGCACGACGACCCGCAGCCCGCGAGGGGCATCCGTCCAGGGACAACGCTCCG
GCCAGAGCGGACGAGATCTG

Downstream 100 bases:

>100_bases
TGAGACTTCTCTGCGCGCTTGTCGGATTGCTCTGGGCCGCGGCCGCCGCGGCACAGGATTTCAGCGCCGTCGCCCGGCTG
ACCGACGCCTCGGTGGGCCG

Product: serine/threonine protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTEPRLPNGTRIAGHVITDQIGRGGFGITYRANVLETGATVALKEYFPCDMAHRDAQGFVRPLPRAERMYEEGLRAFITE
ANILKALPQQAGLVRVRGAFEKFGTAYCAMDFIEGEPLSRLAQATLQRTGFIAEDLLRSFLAPICLALETVHSTNLVHRD
VKPANIMISRRDQQPILIDFGAARPSGQRGSLAMFTRSYAPVELFPRAQVRSAQPLEEGPWSDIYSLSVMLYEMATRQLP
PSAPDRAAAVAAGAPDPYVPLTALLAQRRLRGSYSPAFASAVDAGCALWPEARPRTVRQFAYMAGVEMGSAPPPTPRRTV
TGAAPSPSSSVPRSQRRPVNRQSIAMVLLMLLIAGAAVSYGVVSRQPDWVAYR

Sequences:

>Translated_373_residues
MTEPRLPNGTRIAGHVITDQIGRGGFGITYRANVLETGATVALKEYFPCDMAHRDAQGFVRPLPRAERMYEEGLRAFITE
ANILKALPQQAGLVRVRGAFEKFGTAYCAMDFIEGEPLSRLAQATLQRTGFIAEDLLRSFLAPICLALETVHSTNLVHRD
VKPANIMISRRDQQPILIDFGAARPSGQRGSLAMFTRSYAPVELFPRAQVRSAQPLEEGPWSDIYSLSVMLYEMATRQLP
PSAPDRAAAVAAGAPDPYVPLTALLAQRRLRGSYSPAFASAVDAGCALWPEARPRTVRQFAYMAGVEMGSAPPPTPRRTV
TGAAPSPSSSVPRSQRRPVNRQSIAMVLLMLLIAGAAVSYGVVSRQPDWVAYR
>Mature_372_residues
TEPRLPNGTRIAGHVITDQIGRGGFGITYRANVLETGATVALKEYFPCDMAHRDAQGFVRPLPRAERMYEEGLRAFITEA
NILKALPQQAGLVRVRGAFEKFGTAYCAMDFIEGEPLSRLAQATLQRTGFIAEDLLRSFLAPICLALETVHSTNLVHRDV
KPANIMISRRDQQPILIDFGAARPSGQRGSLAMFTRSYAPVELFPRAQVRSAQPLEEGPWSDIYSLSVMLYEMATRQLPP
SAPDRAAAVAAGAPDPYVPLTALLAQRRLRGSYSPAFASAVDAGCALWPEARPRTVRQFAYMAGVEMGSAPPPTPRRTVT
GAAPSPSSSVPRSQRRPVNRQSIAMVLLMLLIAGAAVSYGVVSRQPDWVAYR

Specific function: Probably required for both normal cellular growth and differentiation. Inactivation of pknA leads to colonies that appear light green and rough in the absence of combined nitrogen [H]

COG id: COG0515

COG function: function code RTKL; Serine/threonine protein kinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI207029299, Length=189, Percent_Identity=26.4550264550265, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI41872673, Length=222, Percent_Identity=29.2792792792793, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI19424132, Length=232, Percent_Identity=28.448275862069, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI282402177, Length=212, Percent_Identity=29.7169811320755, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI217330634, Length=225, Percent_Identity=28, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI89903018, Length=225, Percent_Identity=28.4444444444444, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI30795215, Length=179, Percent_Identity=30.1675977653631, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI261244921, Length=232, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI282402175, Length=179, Percent_Identity=30.1675977653631, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI261244929, Length=232, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI222418622, Length=232, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI261244925, Length=232, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI57165436, Length=232, Percent_Identity=27.1551724137931, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI261244927, Length=232, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI261244923, Length=232, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI19923407, Length=232, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI86562808, Length=326, Percent_Identity=23.3128834355828, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508247, Length=220, Percent_Identity=28.1818181818182, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI72000923, Length=256, Percent_Identity=26.171875, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI72000919, Length=256, Percent_Identity=26.171875, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI72000921, Length=256, Percent_Identity=26.171875, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325104, Length=239, Percent_Identity=27.6150627615063, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321894, Length=227, Percent_Identity=27.3127753303965, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319328, Length=242, Percent_Identity=26.8595041322314, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324805, Length=204, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR017441
- InterPro:   IPR017442
- InterPro:   IPR008271 [H]

Pfam domain/function: PF00069 Pkinase [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 40680; Mature: 40549

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS00108 PROTEIN_KINASE_ST ; PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPRLPNGTRIAGHVITDQIGRGGFGITYRANVLETGATVALKEYFPCDMAHRDAQGFV
CCCCCCCCCCEEEHHHHHHHCCCCCCCEEEECHHHHCCCCEEHHHHCCCCHHHCCHHHHH
RPLPRAERMYEEGLRAFITEANILKALPQQAGLVRVRGAFEKFGTAYCAMDFIEGEPLSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCHHHH
LAQATLQRTGFIAEDLLRSFLAPICLALETVHSTNLVHRDVKPANIMISRRDQQPILIDF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
GAARPSGQRGSLAMFTRSYAPVELFPRAQVRSAQPLEEGPWSDIYSLSVMLYEMATRQLP
CCCCCCCCCCCEEEEECCCCCCEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PSAPDRAAAVAAGAPDPYVPLTALLAQRRLRGSYSPAFASAVDAGCALWPEARPRTVRQF
CCCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
AYMAGVEMGSAPPPTPRRTVTGAAPSPSSSVPRSQRRPVNRQSIAMVLLMLLIAGAAVSY
HHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVVSRQPDWVAYR
HHHCCCCCCCCCC
>Mature Secondary Structure 
TEPRLPNGTRIAGHVITDQIGRGGFGITYRANVLETGATVALKEYFPCDMAHRDAQGFV
CCCCCCCCCEEEHHHHHHHCCCCCCCEEEECHHHHCCCCEEHHHHCCCCHHHCCHHHHH
RPLPRAERMYEEGLRAFITEANILKALPQQAGLVRVRGAFEKFGTAYCAMDFIEGEPLSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCHHHH
LAQATLQRTGFIAEDLLRSFLAPICLALETVHSTNLVHRDVKPANIMISRRDQQPILIDF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
GAARPSGQRGSLAMFTRSYAPVELFPRAQVRSAQPLEEGPWSDIYSLSVMLYEMATRQLP
CCCCCCCCCCCEEEEECCCCCCEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PSAPDRAAAVAAGAPDPYVPLTALLAQRRLRGSYSPAFASAVDAGCALWPEARPRTVRQF
CCCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
AYMAGVEMGSAPPPTPRRTVTGAAPSPSSSVPRSQRRPVNRQSIAMVLLMLLIAGAAVSY
HHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVVSRQPDWVAYR
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7505448; 11759840 [H]