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The map label for this gene is eptA [H]

Identifier: 77465099

GI number: 77465099

Start: 130099

End: 131748

Strand: Direct

Name: eptA [H]

Synonym: RSP_3085

Alternate gene names: 77465099

Gene position: 130099-131748 (Clockwise)

Preceding gene: 77465096

Following gene: 77465100

Centisome position: 13.8

GC content: 69.03

Gene sequence:

>1650_bases
ATGTTCCCATTTCCGCTCAGCGTCGGCCGGGCTCCGGCAACGGCTCCCGCACGGGGTCGCGCGCTTCCCTCCGTCTTCCA
GATCAATCTTGCGGTGACGAGCTTCCTGCTGATCGCGGACAATGCGACCTTCTGGGCCCGTGCGCTCGGGATCTTCGGGC
CGGGGGCAGAGCTTCTGCTGTTCGGAACGGCGATCTGGGCGCTCACCTTCTTCATCGTCGCCCTGTTCGGGACGGGGCCG
CTGCGCCGGCCGATGCTGGCGCTGCTGCTGCTGCTGGGCGCCGCGACCGGTTACTTCCAGGATCGGCTGGGCGTGACGAT
GGACCGGGACATGATCGAGAACGTGATGACGACGACGCTGTCCGAGGGGCGCCATCTCGTCACGCCGGGCTTCCTGATCC
ATCTGGCGATCTTCGGCCTCTTGCCCGCGCTGGTCGTGCTGCGCCTGCCCGTGCGCGAGGGCGGCTGGCGGGCGGCGGCG
CGCAGCGGGCTTTCGGCGGTCATGGCGCTCGCCCTCGGGGCGGGTCTCGTGATGGCCGACTTCAAGACCCTCTCGGCGGT
CCTGCGCGAGCACAAGGAGCTGGTGTCGGCCTGGCAGCCCGCGATGCCGCTCGGCTCGGCGCTGCGCTATGCCAAGCTGC
GCGTGCGCACCCACGATCTGACGGTGGCGGCGCTTGGCACCGATGCGCAGAAGGGGCCGCTCCTCGCGGCGGCGCCGAAG
CCGGTTCTGACCGTCCTCGTGGTGGGCGAGACCGCGCGGGCGCAGAACTGGGGGCTGAACGGCTACGAGCGCGACACGAC
GCCCAAGCTGCGCGCCCGCGGCGTGGTGAATTTCTCCGACGTCGAGAGCTGCGGCACGGCGACGGCCGTGTCGATGCCCT
GCATGTTCTCGAACCTCACGCGCAAGGGCTACAGCCACGAGAAGGGCCTCGCGCAGGAGAACCTGCTCGATGTGCTGGCC
CATGCGGGCGTGGCGGTGGAGTGGTGGGACAACAACACGGGCGACAAGGACATCGCGGCGCGCCTGCCGTCGGCGCGGGT
GCCCGAGACGGTGGATGCCTGCGGCGAGGGCGAATGCACCGATGCGGCCTTCCTGCCGCTCCTCGACCGGACGCTCGCCG
GCATGAAGGACGACACGGTGTTGGTGCTGCACCAGATCGGCAGCCACGGGCCCGCCTATCACCTGCGCTACCCCAAGGCC
TTCGAGCGGTTCTCGCCCGCCTGCCAGAGCGCGGAATTCTCGCGCTGCACGGACGAGGAGATCCGCAATGCCTATGACAA
CAGCCTGGCCTTCACCGATCATATCCTCGCCGCGATGATCGACCGGCTGGCCGCGCAGGACCGCGTGATCCCGGCGCTGG
TCTATGTCTCGGATCACGGCGAGTCCCTGGGCGAGAACGGGCTCTATCTGCATGGCGCGCCGCGCTTCATGGCGCCCGAC
ACGCAGACCCATGTGCCGATGGTGATGTGGCTCTCCGAGGCGTTCCGGTCGACCATGCGCCTCGATGTGGGCTGCCTGCA
GGCGCATGCGGCCGAGCCCGCAAGCCATGACAACCTGTTCCATTCGGTGCTCGGGCTGATGGACATCCGCACCGAGGTGC
GCGACACGAGCCTCGACCGTGTCTCGTCCTGCCGCGCTTCCGCTTCCTGA

Upstream 100 bases:

>100_bases
CGAGCCAGGGCCTCGGTCGCGCAGGGCGACACTGCGACATTTCAGCCTGTTGTCAGCTTGCGCCGATAGCCGGGGCGCAA
CGGGCAAGAGGTCTCAGCAC

Downstream 100 bases:

>100_bases
GGGCCCGCCCATGACCGACCGCATCGATCTGCCCCGGCCTCCGCAGGCTCAGGGCGCCCGCCATCTCCTCGCCTCGGCCC
GTCATTCGCTGGCCGGCCTG

Product: phosphatidylethanolamine:Kdo2-lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MFPFPLSVGRAPATAPARGRALPSVFQINLAVTSFLLIADNATFWARALGIFGPGAELLLFGTAIWALTFFIVALFGTGP
LRRPMLALLLLLGAATGYFQDRLGVTMDRDMIENVMTTTLSEGRHLVTPGFLIHLAIFGLLPALVVLRLPVREGGWRAAA
RSGLSAVMALALGAGLVMADFKTLSAVLREHKELVSAWQPAMPLGSALRYAKLRVRTHDLTVAALGTDAQKGPLLAAAPK
PVLTVLVVGETARAQNWGLNGYERDTTPKLRARGVVNFSDVESCGTATAVSMPCMFSNLTRKGYSHEKGLAQENLLDVLA
HAGVAVEWWDNNTGDKDIAARLPSARVPETVDACGEGECTDAAFLPLLDRTLAGMKDDTVLVLHQIGSHGPAYHLRYPKA
FERFSPACQSAEFSRCTDEEIRNAYDNSLAFTDHILAAMIDRLAAQDRVIPALVYVSDHGESLGENGLYLHGAPRFMAPD
TQTHVPMVMWLSEAFRSTMRLDVGCLQAHAAEPASHDNLFHSVLGLMDIRTEVRDTSLDRVSSCRASAS

Sequences:

>Translated_549_residues
MFPFPLSVGRAPATAPARGRALPSVFQINLAVTSFLLIADNATFWARALGIFGPGAELLLFGTAIWALTFFIVALFGTGP
LRRPMLALLLLLGAATGYFQDRLGVTMDRDMIENVMTTTLSEGRHLVTPGFLIHLAIFGLLPALVVLRLPVREGGWRAAA
RSGLSAVMALALGAGLVMADFKTLSAVLREHKELVSAWQPAMPLGSALRYAKLRVRTHDLTVAALGTDAQKGPLLAAAPK
PVLTVLVVGETARAQNWGLNGYERDTTPKLRARGVVNFSDVESCGTATAVSMPCMFSNLTRKGYSHEKGLAQENLLDVLA
HAGVAVEWWDNNTGDKDIAARLPSARVPETVDACGEGECTDAAFLPLLDRTLAGMKDDTVLVLHQIGSHGPAYHLRYPKA
FERFSPACQSAEFSRCTDEEIRNAYDNSLAFTDHILAAMIDRLAAQDRVIPALVYVSDHGESLGENGLYLHGAPRFMAPD
TQTHVPMVMWLSEAFRSTMRLDVGCLQAHAAEPASHDNLFHSVLGLMDIRTEVRDTSLDRVSSCRASAS
>Mature_549_residues
MFPFPLSVGRAPATAPARGRALPSVFQINLAVTSFLLIADNATFWARALGIFGPGAELLLFGTAIWALTFFIVALFGTGP
LRRPMLALLLLLGAATGYFQDRLGVTMDRDMIENVMTTTLSEGRHLVTPGFLIHLAIFGLLPALVVLRLPVREGGWRAAA
RSGLSAVMALALGAGLVMADFKTLSAVLREHKELVSAWQPAMPLGSALRYAKLRVRTHDLTVAALGTDAQKGPLLAAAPK
PVLTVLVVGETARAQNWGLNGYERDTTPKLRARGVVNFSDVESCGTATAVSMPCMFSNLTRKGYSHEKGLAQENLLDVLA
HAGVAVEWWDNNTGDKDIAARLPSARVPETVDACGEGECTDAAFLPLLDRTLAGMKDDTVLVLHQIGSHGPAYHLRYPKA
FERFSPACQSAEFSRCTDEEIRNAYDNSLAFTDHILAAMIDRLAAQDRVIPALVYVSDHGESLGENGLYLHGAPRFMAPD
TQTHVPMVMWLSEAFRSTMRLDVGCLQAHAAEPASHDNLFHSVLGLMDIRTEVRDTSLDRVSSCRASAS

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=471, Percent_Identity=39.4904458598726, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI87082286, Length=477, Percent_Identity=26.8343815513627, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI87082223, Length=255, Percent_Identity=29.0196078431373, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59296; Mature: 59296

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPFPLSVGRAPATAPARGRALPSVFQINLAVTSFLLIADNATFWARALGIFGPGAELLL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCHHHHH
FGTAIWALTFFIVALFGTGPLRRPMLALLLLLGAATGYFQDRLGVTMDRDMIENVMTTTL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHCCCEECHHHHHHHHHHHH
SEGRHLVTPGFLIHLAIFGLLPALVVLRLPVREGGWRAAARSGLSAVMALALGAGLVMAD
HCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
FKTLSAVLREHKELVSAWQPAMPLGSALRYAKLRVRTHDLTVAALGTDAQKGPLLAAAPK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCEEEEEECCCCCCCCEEEECCC
PVLTVLVVGETARAQNWGLNGYERDTTPKLRARGVVNFSDVESCGTATAVSMPCMFSNLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHH
RKGYSHEKGLAQENLLDVLAHAGVAVEWWDNNTGDKDIAARLPSARVPETVDACGEGECT
HCCCCHHCCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCCCCCHHHHHCCCCCCC
DAAFLPLLDRTLAGMKDDTVLVLHQIGSHGPAYHLRYPKAFERFSPACQSAEFSRCTDEE
CHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCEEEECCCHHHHHHCHHHCCCCHHCCCHHH
IRNAYDNSLAFTDHILAAMIDRLAAQDRVIPALVYVSDHGESLGENGLYLHGAPRFMAPD
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCC
TQTHVPMVMWLSEAFRSTMRLDVGCLQAHAAEPASHDNLFHSVLGLMDIRTEVRDTSLDR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSSCRASAS
HHHHHCCCC
>Mature Secondary Structure
MFPFPLSVGRAPATAPARGRALPSVFQINLAVTSFLLIADNATFWARALGIFGPGAELLL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCHHHHH
FGTAIWALTFFIVALFGTGPLRRPMLALLLLLGAATGYFQDRLGVTMDRDMIENVMTTTL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHCCCEECHHHHHHHHHHHH
SEGRHLVTPGFLIHLAIFGLLPALVVLRLPVREGGWRAAARSGLSAVMALALGAGLVMAD
HCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
FKTLSAVLREHKELVSAWQPAMPLGSALRYAKLRVRTHDLTVAALGTDAQKGPLLAAAPK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCEEEEEECCCCCCCCEEEECCC
PVLTVLVVGETARAQNWGLNGYERDTTPKLRARGVVNFSDVESCGTATAVSMPCMFSNLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHH
RKGYSHEKGLAQENLLDVLAHAGVAVEWWDNNTGDKDIAARLPSARVPETVDACGEGECT
HCCCCHHCCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCCCCCHHHHHCCCCCCC
DAAFLPLLDRTLAGMKDDTVLVLHQIGSHGPAYHLRYPKAFERFSPACQSAEFSRCTDEE
CHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCEEEECCCHHHHHHCHHHCCCCHHCCCHHH
IRNAYDNSLAFTDHILAAMIDRLAAQDRVIPALVYVSDHGESLGENGLYLHGAPRFMAPD
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCC
TQTHVPMVMWLSEAFRSTMRLDVGCLQAHAAEPASHDNLFHSVLGLMDIRTEVRDTSLDR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSSCRASAS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]