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The map label for this gene is opuD [H]

Identifier: 77465094

GI number: 77465094

Start: 122532

End: 124187

Strand: Reverse

Name: opuD [H]

Synonym: RSP_3080

Alternate gene names: 77465094

Gene position: 124187-122532 (Counterclockwise)

Preceding gene: 77465095

Following gene: 77465092

Centisome position: 13.17

GC content: 66.73

Gene sequence:

>1656_bases
ATGGCCGATGCCACGATAAGAACCGAGCCGCTGCCCGAGGAGGCGATACCCGCCCCCGAAGGCCCCACCCAGATCATCGA
CACCGACTACGAGATCGGTCAGGACAACATCAGCTACCGACGACGCTTCGTCTTCGAACTCGACATCCACAACGTCGTCT
TCAGCGTGTCGGCCCTGACCATCGTGGCCTTCACCTTCCTCACGCTGATGTTCCAGACCACGCTCGGTCCGGTCTTCGCC
GCCGTCCGCGACGCGCTGACCTCGAACCTCGCCTGGTTCTTCCTGCTGGCGGGCAATGTCTTCGTCCTGCTCTGCGTCGT
GCTGATCTTCCTGCCGCTGGGCCGGATCCGGCTGGGCGGACCCGACGCCACGCCCGACTACAGCCGCCTCTCGTGGTTCT
CGATGCTGTTCGCGGCCGGCATGGGCATCGGCCTCATGTTCTACGGCGTGTCCGAGCCGCTGGGCAACTACACCGCCGCC
TTCACCGGCCCGGTGTTCGAGAACGGCGTGCGCACCGACTGGTCGCCGCTCAACGGGGCGCCCGGCGATACCGAGGCCGC
CCGGCGTCTCGCCATGGCGGCCACGATCTTCCACTGGGCGCTGCACCCGTGGGCGATCTACGCGGTCGTCGCCCTCTCGC
TCGCGCTCTTTGCCTACAACAAGGGCCTGCCGCTGACGCTGCGCTCGGTCTTCTATCCGATCTTCGGCGAGCGCGTCTGG
GGCTGGCCGGGCCATGTCATCGACATCCTCGCCGTCTTCGCCACGATCTTCGGCCTCTCGACCTCGCTCGGGATCGGCGC
GCAGCAGGCGGGGGCCGGGCTCGACTTCCTGTTCGGCCTGCCCTCGACCGTCGGGGTGACCGTCTTTCTCGTGATCGTCA
TCACCGGCATCGCGACGGCTTCTGTCATCGCGGGCATGGACAAGGGCGTGAAACGCCTGTCCGAGGCGAACATGGCGCTG
GCCTTCCTGCTGCTGATGTTCGTGATCCTCGTCGGGCCCACGCTGCAGATCGTGCGCGGCTTCTTCCTGAACCTTCTGGC
TTATGTGGAATATCTGCCGGCGCTGTCGAACCCGTTCGGGCGCACCGACGACAACTTCCGCCACGGCTGGACCGCCTTCT
ACTGGGCCTGGTGGATCAGCTGGTCGCCGTTCGTGGGCATGTTCATCGCCCGTGTGAGCCGCGGCCGCTCGGTGCGCGAG
TTCCTGATCGCGGTGCTGCTCATCCCGTCCATCGTCTCGACGATCTGGATGACCGCCCTGGGCGGCACCGCCATCAGTCA
GGTGGTGAACGAGGGGCTGAGCTCGGTGCAGGATGCAGCCCTCGAGATCCAGCTCTTCGAGATGCTCTCGCACCTGCCGC
TCACGCAGATCACCTCGATGATCGGGATCGTGCTGGTGATCGTCTTCTTCGTCACCTCGTCGGACTCGGGCTCGCTGGTG
ATCGACACGATCGCGGCGGGCGGCAAGGTCAATGCGCCGGTGCCGCAGCGGGTGTTCTGGGCCACCTTCGAGGGGCTGGT
GGCCATCGCGCTCCTGCTCGGCGGGGGGCTGGCCGCGCTGCAGGCGATGGCGGTCTCAACCGGCTTCCCCTTCACCATCG
TGCTGCTCGGCGCCTGCTACGCCCTGATCCGGGGTCTTGCGGCCGAACCGCGCTGA

Upstream 100 bases:

>100_bases
CATTCCGCGTGCCCGGGAGAATTGCGCGCCACCGGAGGGACCTCAGCGGGCTTTGGCCTCCGTCCCTTCGCACGACCGAC
ATGACCGGAGGGACCGACCG

Downstream 100 bases:

>100_bases
CAGAAACCGGCGCGCCTTAAGGGGCGCGCCGGATGCTCTTCCGGGCGCCCGGCGCTCAGCCCTTCCCAAGCGCCGCGATG
AGCTGATCGAGCACCGCGGT

Product: BCCT family choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MADATIRTEPLPEEAIPAPEGPTQIIDTDYEIGQDNISYRRRFVFELDIHNVVFSVSALTIVAFTFLTLMFQTTLGPVFA
AVRDALTSNLAWFFLLAGNVFVLLCVVLIFLPLGRIRLGGPDATPDYSRLSWFSMLFAAGMGIGLMFYGVSEPLGNYTAA
FTGPVFENGVRTDWSPLNGAPGDTEAARRLAMAATIFHWALHPWAIYAVVALSLALFAYNKGLPLTLRSVFYPIFGERVW
GWPGHVIDILAVFATIFGLSTSLGIGAQQAGAGLDFLFGLPSTVGVTVFLVIVITGIATASVIAGMDKGVKRLSEANMAL
AFLLLMFVILVGPTLQIVRGFFLNLLAYVEYLPALSNPFGRTDDNFRHGWTAFYWAWWISWSPFVGMFIARVSRGRSVRE
FLIAVLLIPSIVSTIWMTALGGTAISQVVNEGLSSVQDAALEIQLFEMLSHLPLTQITSMIGIVLVIVFFVTSSDSGSLV
IDTIAAGGKVNAPVPQRVFWATFEGLVAIALLLGGGLAALQAMAVSTGFPFTIVLLGACYALIRGLAAEPR

Sequences:

>Translated_551_residues
MADATIRTEPLPEEAIPAPEGPTQIIDTDYEIGQDNISYRRRFVFELDIHNVVFSVSALTIVAFTFLTLMFQTTLGPVFA
AVRDALTSNLAWFFLLAGNVFVLLCVVLIFLPLGRIRLGGPDATPDYSRLSWFSMLFAAGMGIGLMFYGVSEPLGNYTAA
FTGPVFENGVRTDWSPLNGAPGDTEAARRLAMAATIFHWALHPWAIYAVVALSLALFAYNKGLPLTLRSVFYPIFGERVW
GWPGHVIDILAVFATIFGLSTSLGIGAQQAGAGLDFLFGLPSTVGVTVFLVIVITGIATASVIAGMDKGVKRLSEANMAL
AFLLLMFVILVGPTLQIVRGFFLNLLAYVEYLPALSNPFGRTDDNFRHGWTAFYWAWWISWSPFVGMFIARVSRGRSVRE
FLIAVLLIPSIVSTIWMTALGGTAISQVVNEGLSSVQDAALEIQLFEMLSHLPLTQITSMIGIVLVIVFFVTSSDSGSLV
IDTIAAGGKVNAPVPQRVFWATFEGLVAIALLLGGGLAALQAMAVSTGFPFTIVLLGACYALIRGLAAEPR
>Mature_550_residues
ADATIRTEPLPEEAIPAPEGPTQIIDTDYEIGQDNISYRRRFVFELDIHNVVFSVSALTIVAFTFLTLMFQTTLGPVFAA
VRDALTSNLAWFFLLAGNVFVLLCVVLIFLPLGRIRLGGPDATPDYSRLSWFSMLFAAGMGIGLMFYGVSEPLGNYTAAF
TGPVFENGVRTDWSPLNGAPGDTEAARRLAMAATIFHWALHPWAIYAVVALSLALFAYNKGLPLTLRSVFYPIFGERVWG
WPGHVIDILAVFATIFGLSTSLGIGAQQAGAGLDFLFGLPSTVGVTVFLVIVITGIATASVIAGMDKGVKRLSEANMALA
FLLLMFVILVGPTLQIVRGFFLNLLAYVEYLPALSNPFGRTDDNFRHGWTAFYWAWWISWSPFVGMFIARVSRGRSVREF
LIAVLLIPSIVSTIWMTALGGTAISQVVNEGLSSVQDAALEIQLFEMLSHLPLTQITSMIGIVLVIVFFVTSSDSGSLVI
DTIAAGGKVNAPVPQRVFWATFEGLVAIALLLGGGLAALQAMAVSTGFPFTIVLLGACYALIRGLAAEPR

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=521, Percent_Identity=35.700575815739, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1788102, Length=448, Percent_Identity=28.7946428571429, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1786224, Length=448, Percent_Identity=26.7857142857143, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 59503; Mature: 59372

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADATIRTEPLPEEAIPAPEGPTQIIDTDYEIGQDNISYRRRFVFELDIHNVVFSVSALT
CCCCCCCCCCCCHHCCCCCCCCCCEECCCHHCCCCCHHHHHHEEEEEEHHHHHHHHHHHH
IVAFTFLTLMFQTTLGPVFAAVRDALTSNLAWFFLLAGNVFVLLCVVLIFLPLGRIRLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
PDATPDYSRLSWFSMLFAAGMGIGLMFYGVSEPLGNYTAAFTGPVFENGVRTDWSPLNGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCCCCCCCCC
PGDTEAARRLAMAATIFHWALHPWAIYAVVALSLALFAYNKGLPLTLRSVFYPIFGERVW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
GWPGHVIDILAVFATIFGLSTSLGIGAQQAGAGLDFLFGLPSTVGVTVFLVIVITGIATA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
SVIAGMDKGVKRLSEANMALAFLLLMFVILVGPTLQIVRGFFLNLLAYVEYLPALSNPFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RTDDNFRHGWTAFYWAWWISWSPFVGMFIARVSRGRSVREFLIAVLLIPSIVSTIWMTAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGTAISQVVNEGLSSVQDAALEIQLFEMLSHLPLTQITSMIGIVLVIVFFVTSSDSGSLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
IDTIAAGGKVNAPVPQRVFWATFEGLVAIALLLGGGLAALQAMAVSTGFPFTIVLLGACY
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHH
ALIRGLAAEPR
HHHHHHCCCCC
>Mature Secondary Structure 
ADATIRTEPLPEEAIPAPEGPTQIIDTDYEIGQDNISYRRRFVFELDIHNVVFSVSALT
CCCCCCCCCCCHHCCCCCCCCCCEECCCHHCCCCCHHHHHHEEEEEEHHHHHHHHHHHH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HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]