Click here to switch to the map view.

The map label for this gene is bhuA [H]

Identifier: 77465071

GI number: 77465071

Start: 94964

End: 96898

Strand: Reverse

Name: bhuA [H]

Synonym: RSP_3056

Alternate gene names: 77465071

Gene position: 96898-94964 (Counterclockwise)

Preceding gene: 77465072

Following gene: 77465069

Centisome position: 10.28

GC content: 68.27

Gene sequence:

>1935_bases
ATGTCCATCCTGTCGCCCCGAACGATCCGGCTTCTGGCCGGGAGCGCCGCCCTGAGCGGGTCCTGCGTCGCCGCCCATGC
GCAGGATCTGGCCTTCTCGCTCGATCCCATCGTGGTGCAGGCGCGCGACGATTTCGCCGAAGCCGCCGACCGCGCCACCT
CGATGTATGTCGCCGATGCCGAGCTGGAGCGCGCGCGCACCGGAGACCTCAAGGACGTCTTCGCGGGCATCGCCTCTGTC
TCGGTCGGAGGGGCGCTGCCTCTCACGCAGAAGATCTTCGTGAACGGCGTCGACATGCTGAATCTCGGCGTCAGCATCGA
CGGGGCCGCGCAGAACAACCGCGCCTTCCACCATGTCTCGGCCAATGCGATCGATCCGGGGCTGCTCAAGCAGGTGCGCG
TGGATGCCACGATCTCGCCGGCCGATGCCGGGCCCCATGCGCTCGCGGGCTCGGTCGTGTTCGAGACGGTCGATGCCGCC
GACATCCTCGGCGAGGTGCAGCGCTTCGGCGGCACCCTGCGGCTGAGCTACGGCGACAACGGCAAGACGGCGCAGGGCGC
CCTGACCCTCGCCGGGCGCGAGGGCGGCTTCGAGTGGCTGGCTTACGCCAAGCGCGCCACGGGCGACGATTACGAGGATG
GAGACGGGGCCACGCGGCTCGGGACGGCGGCCAATCTCAAGAGCGCGCTGGGAAAGCTCGCCTATGAGAGCGACGGCGGC
CACCGGTTCGAGCTGTCGGCGCTGCGCCTGAGGGACGACGAGCTGCGCCAGTTCCGCGCGAATTTCGGCGGTCTGGGCGG
GGTGGTCGATCAGCTGCGCGTCTACGACACGACGCGGGAAAGCTGGTCGCTCTCCTACGAGAACACGCAAGGCGAGGGGA
TGTGGGATCCGAGCCTCCGGCTCGGCTATTCGGAGAGCGACGTCGTCATTCCCCTGCCCTACGACAGCAACGGCCTGTCG
GGCACATGGTCGGCCACGCTGCAGAACGATTTTCACCTGAACGCCACCGACCGGATCTCGGCCGGCCTCGACTGGCAGCG
CCGGTTCGGGGAATATTCGAGCCCGACCTTCGGCGAGTTCCTCGATGAGACATCGCACAACCTCGGCCTCTTCGTGCAGG
CCCGGCTCGAGCCCACCGATCGCTGGCGCCTTTCGTTCGGCGGCCGCCTCGACCGGCAGGAGTTCGAGGGCGTCGATGGC
AGCGACCTCGGCCACTCGGGGCCCTCGGGCAACCTCTCCGCAAGCTACGCCCTGACCGAGCGCCTCACGTTGCGCGGCGG
CCTGTCCTCGATCTTCGGCGGCATCGACATCGAGGACAATTACATCTTCCGGCCGAGCTGGAGCTACGACAGCCTCCGGC
CGTCGCGGGCGCGGAACGCGAGCCTCGGCTTCGACTGGGACGTGGGCGCCTTCAAGGTCGGAGGCGAGCTCTTCCTGACC
GAGATCAACCGCGCGCGCAGTGCCACCGAAATGTTCGACTTCGAGAGCCGCGGCTTCAACCTCGGCGCGACCTACGGCTG
GGACGGTGGCTTTGCGCGCGTCACCCTCTCCCACAGCGAGGTGAAGGTGGACGGCGCAAAGGCCGCGGGCTACGAGGCGC
TCGACTTCGGCGCCCCTCTGGGCACGGTGGCCGCGGTCGAGCTTCAGCAGGACACGGCAGTTGCGGGGCTGCGGCTCGGC
GGCGGGCTCGATCTCGCGCTCGACCACGACATGCCCGCTACGGCCGAACGGGATCTCGCGGGCTATTCGGTGGTGAACCT
CTTTGCCGAATATGTGCCGCCTGAGGCGCAGAACCTCACGCTCCGGCTGCAGGTCGACAACCTATTCGACAAGACCTATG
CGGACCGCGCGACCTACGGCGCGGAATACAGCGACGTCACCTCGCTGAAGGAGCCGGGCCGGACGATCACGCTCAGCGCG
GTCACGCGCTTCTGA

Upstream 100 bases:

>100_bases
TTAACATGCGATTGCATCTAACGGAGCGTCCCGCTAAGGTGCCATGAAGCCAGGTTTCCCCAGCGCGCATCCGTTTCGCA
TCAGCTGACCCTGGAGACCC

Downstream 100 bases:

>100_bases
GGGCGGCGGTCGCGGCCGTCAGGGATAGAGCCGGATCAGCAGCGCCGCCAGCGCATAGCCCTCTTCATGGCTGAGGCGGG
CGCCGAGCTCTTCGTGGATG

Product: TonB dependent-iron siderophore receptor

Products: NA

Alternate protein names: Brucella heme uptake protein A [H]

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MSILSPRTIRLLAGSAALSGSCVAAHAQDLAFSLDPIVVQARDDFAEAADRATSMYVADAELERARTGDLKDVFAGIASV
SVGGALPLTQKIFVNGVDMLNLGVSIDGAAQNNRAFHHVSANAIDPGLLKQVRVDATISPADAGPHALAGSVVFETVDAA
DILGEVQRFGGTLRLSYGDNGKTAQGALTLAGREGGFEWLAYAKRATGDDYEDGDGATRLGTAANLKSALGKLAYESDGG
HRFELSALRLRDDELRQFRANFGGLGGVVDQLRVYDTTRESWSLSYENTQGEGMWDPSLRLGYSESDVVIPLPYDSNGLS
GTWSATLQNDFHLNATDRISAGLDWQRRFGEYSSPTFGEFLDETSHNLGLFVQARLEPTDRWRLSFGGRLDRQEFEGVDG
SDLGHSGPSGNLSASYALTERLTLRGGLSSIFGGIDIEDNYIFRPSWSYDSLRPSRARNASLGFDWDVGAFKVGGELFLT
EINRARSATEMFDFESRGFNLGATYGWDGGFARVTLSHSEVKVDGAKAAGYEALDFGAPLGTVAAVELQQDTAVAGLRLG
GGLDLALDHDMPATAERDLAGYSVVNLFAEYVPPEAQNLTLRLQVDNLFDKTYADRATYGAEYSDVTSLKEPGRTITLSA
VTRF

Sequences:

>Translated_644_residues
MSILSPRTIRLLAGSAALSGSCVAAHAQDLAFSLDPIVVQARDDFAEAADRATSMYVADAELERARTGDLKDVFAGIASV
SVGGALPLTQKIFVNGVDMLNLGVSIDGAAQNNRAFHHVSANAIDPGLLKQVRVDATISPADAGPHALAGSVVFETVDAA
DILGEVQRFGGTLRLSYGDNGKTAQGALTLAGREGGFEWLAYAKRATGDDYEDGDGATRLGTAANLKSALGKLAYESDGG
HRFELSALRLRDDELRQFRANFGGLGGVVDQLRVYDTTRESWSLSYENTQGEGMWDPSLRLGYSESDVVIPLPYDSNGLS
GTWSATLQNDFHLNATDRISAGLDWQRRFGEYSSPTFGEFLDETSHNLGLFVQARLEPTDRWRLSFGGRLDRQEFEGVDG
SDLGHSGPSGNLSASYALTERLTLRGGLSSIFGGIDIEDNYIFRPSWSYDSLRPSRARNASLGFDWDVGAFKVGGELFLT
EINRARSATEMFDFESRGFNLGATYGWDGGFARVTLSHSEVKVDGAKAAGYEALDFGAPLGTVAAVELQQDTAVAGLRLG
GGLDLALDHDMPATAERDLAGYSVVNLFAEYVPPEAQNLTLRLQVDNLFDKTYADRATYGAEYSDVTSLKEPGRTITLSA
VTRF
>Mature_643_residues
SILSPRTIRLLAGSAALSGSCVAAHAQDLAFSLDPIVVQARDDFAEAADRATSMYVADAELERARTGDLKDVFAGIASVS
VGGALPLTQKIFVNGVDMLNLGVSIDGAAQNNRAFHHVSANAIDPGLLKQVRVDATISPADAGPHALAGSVVFETVDAAD
ILGEVQRFGGTLRLSYGDNGKTAQGALTLAGREGGFEWLAYAKRATGDDYEDGDGATRLGTAANLKSALGKLAYESDGGH
RFELSALRLRDDELRQFRANFGGLGGVVDQLRVYDTTRESWSLSYENTQGEGMWDPSLRLGYSESDVVIPLPYDSNGLSG
TWSATLQNDFHLNATDRISAGLDWQRRFGEYSSPTFGEFLDETSHNLGLFVQARLEPTDRWRLSFGGRLDRQEFEGVDGS
DLGHSGPSGNLSASYALTERLTLRGGLSSIFGGIDIEDNYIFRPSWSYDSLRPSRARNASLGFDWDVGAFKVGGELFLTE
INRARSATEMFDFESRGFNLGATYGWDGGFARVTLSHSEVKVDGAKAAGYEALDFGAPLGTVAAVELQQDTAVAGLRLGG
GLDLALDHDMPATAERDLAGYSVVNLFAEYVPPEAQNLTLRLQVDNLFDKTYADRATYGAEYSDVTSLKEPGRTITLSAV
TRF

Specific function: Heme transporter playing an important role in stationary-phase iron acquisition and required for maintenance of chronic infection in mice [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tonB-dependent receptor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 69202; Mature: 69071

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILSPRTIRLLAGSAALSGSCVAAHAQDLAFSLDPIVVQARDDFAEAADRATSMYVADA
CCCCCCCEEEEEECCCCCCCCEEEHHHHHHEEECCCEEEEECHHHHHHHHHHHEEEEECC
ELERARTGDLKDVFAGIASVSVGGALPLTQKIFVNGVDMLNLGVSIDGAAQNNRAFHHVS
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECEEECCCCCCCCEEEEEE
ANAIDPGLLKQVRVDATISPADAGPHALAGSVVFETVDAADILGEVQRFGGTLRLSYGDN
CCCCCHHHHHHEEECEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
GKTAQGALTLAGREGGFEWLAYAKRATGDDYEDGDGATRLGTAANLKSALGKLAYESDGG
CCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHEEECCCC
HRFELSALRLRDDELRQFRANFGGLGGVVDQLRVYDTTRESWSLSYENTQGEGMWDPSLR
CEEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCEE
LGYSESDVVIPLPYDSNGLSGTWSATLQNDFHLNATDRISAGLDWQRRFGEYSSPTFGEF
ECCCCCCEEEECCCCCCCCCCEEEEEECCCEEECCHHHHCCCCCHHHHCCCCCCCHHHHH
LDETSHNLGLFVQARLEPTDRWRLSFGGRLDRQEFEGVDGSDLGHSGPSGNLSASYALTE
HHHCCCCCEEEEEEECCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEE
RLTLRGGLSSIFGGIDIEDNYIFRPSWSYDSLRPSRARNASLGFDWDVGAFKVGGELFLT
HEEECCCHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEE
EINRARSATEMFDFESRGFNLGATYGWDGGFARVTLSHSEVKVDGAKAAGYEALDFGAPL
EHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEECCEEEECCCCCCCCEEEECCCCC
GTVAAVELQQDTAVAGLRLGGGLDLALDHDMPATAERDLAGYSVVNLFAEYVPPEAQNLT
CCEEEEEEECCCEEEEEEECCCEEEEECCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCEE
LRLQVDNLFDKTYADRATYGAEYSDVTSLKEPGRTITLSAVTRF
EEEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
SILSPRTIRLLAGSAALSGSCVAAHAQDLAFSLDPIVVQARDDFAEAADRATSMYVADA
CCCCCCEEEEEECCCCCCCCEEEHHHHHHEEECCCEEEEECHHHHHHHHHHHEEEEECC
ELERARTGDLKDVFAGIASVSVGGALPLTQKIFVNGVDMLNLGVSIDGAAQNNRAFHHVS
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECEEECCCCCCCCEEEEEE
ANAIDPGLLKQVRVDATISPADAGPHALAGSVVFETVDAADILGEVQRFGGTLRLSYGDN
CCCCCHHHHHHEEECEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
GKTAQGALTLAGREGGFEWLAYAKRATGDDYEDGDGATRLGTAANLKSALGKLAYESDGG
CCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHEEECCCC
HRFELSALRLRDDELRQFRANFGGLGGVVDQLRVYDTTRESWSLSYENTQGEGMWDPSLR
CEEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCEE
LGYSESDVVIPLPYDSNGLSGTWSATLQNDFHLNATDRISAGLDWQRRFGEYSSPTFGEF
ECCCCCCEEEECCCCCCCCCCEEEEEECCCEEECCHHHHCCCCCHHHHCCCCCCCHHHHH
LDETSHNLGLFVQARLEPTDRWRLSFGGRLDRQEFEGVDGSDLGHSGPSGNLSASYALTE
HHHCCCCCEEEEEEECCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEE
RLTLRGGLSSIFGGIDIEDNYIFRPSWSYDSLRPSRARNASLGFDWDVGAFKVGGELFLT
HEEECCCHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEE
EINRARSATEMFDFESRGFNLGATYGWDGGFARVTLSHSEVKVDGAKAAGYEALDFGAPL
EHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEECCEEEECCCCCCCCEEEECCCCC
GTVAAVELQQDTAVAGLRLGGGLDLALDHDMPATAERDLAGYSVVNLFAEYVPPEAQNLT
CCEEEEEEECCCEEEEEEECCCEEEEECCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCEE
LRLQVDNLFDKTYADRATYGAEYSDVTSLKEPGRTITLSAVTRF
EEEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA