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The map label for this gene is hsaA [H]

Identifier: 77465031

GI number: 77465031

Start: 49518

End: 50690

Strand: Reverse

Name: hsaA [H]

Synonym: RSP_3018

Alternate gene names: 77465031

Gene position: 50690-49518 (Counterclockwise)

Preceding gene: 77465032

Following gene: 77465030

Centisome position: 5.38

GC content: 65.9

Gene sequence:

>1173_bases
ATGTTCGACGATACCACCCCGATGATCGAGCGCGTCCGCGCGATCCTTCCCCGGATTGCGGCGAATGCCTCCAGAGCGGA
GGAGATGCGCGAGGTGCCGAAGGAGAACATCGACCTGCTCAAGAGCACCGGCCTGCACCGCGCCTTCCAGCCGAAGGCCT
ATGGCGGGCTCGAGATGCCGTTGCCGGACTTCGAGAATTGCATCGCGCTGATCGCCACCGCCTGCGGGTCCACCGCCTGG
GCCTTCTCGCTCCTGGCCGAGCACGCGCACCAGATCGCGCTCTATTCGAAGCAATTGCAGGACGAGATCTGGCAGGACGA
TCCCGACACGGTCTGCTCCTCTTCGATCGCCCCTTACGGAAAAGCCCAGGACGTCGAGGGCGGCGTCCGCTTCTCGGGCG
AGTTCGGCTGGTCGTCGGGCTGCGATCACGCGCAATGGGCGATCCTTGGCTTCCTGCGCGACACGCCCGAAGGCGGCAAG
GTCTACAGCTTCGCCATCCTTCCCCGCAGCGACTATGAGATCAAGGACACCTGGTTCACCGCCGGGATGCGGGGCACCGG
CTCGAAGACCCTCGTGGTCAGGGACGCCTTCGTGCCCGAGCACCGGATCGAGACCGTGCCGGCGCTGATGACTCTGACCT
CGGCGGGCGGCGGCCTCTATCCCGGAAGCACCACCTATCATGTGCCCTTCATCTACGTCTTCGCCAGCTGCTTCTCCGCC
GTGTCTCTGGGCATCGCGGAGCGGATGATCCAGCTTTACACCGAGCGGACACGGAACCGCGTCCGCGCCTACACCGGCGC
CAAGGTGAGTCAGTCCATTCCCGCCTGCATGCGTCTGGCGGAAAGCACGCATCAGGTGGCCGCGGGCCGGGCCTTCCTCG
AGAAGACCTGGGAGGACATGCGCGACCACGCCGAGCGCCGCGTCTTTCCGGACGAGACCAGGATGGCCTTCTGGCGGACC
AATCAGGCCTATGCGGTGAAGATGTTCGTCGCTGCGGTGGACCGGCTGTTCGAAGCGTCCGGCGGATCGTCCTGGTTCGA
CGATGCCGAAGGGCAGCGCCTGTTCCGCGACGCGCACATGACTGCCGCCCACGCCTACACCGACTACGACATCTGCGCCC
AGATCCTCGGGCGGGCGCTGATGGGGCTGGAGCGGGATCCCTCCCTGTTCTGA

Upstream 100 bases:

>100_bases
TCGAGGGCCCGGGCCATGTGGCCCTCACATCCGATGCCCGCTTCCGCGAGGCGGCCGTCGAATTCCTGTCCAAGTGACGC
CTCCAGGGAGGAGAAACCCC

Downstream 100 bases:

>100_bases
AACACCCGCCGCCCGGCCCCGCCCCCGGGCCGGGCCGCTCTCCCCTTGCCTCAACCAAACCGCAGAGGTCCCGATGACCG
ATACCTTCCCCGCCGCCAAG

Product: putative acyl-CoA dehydrogenase

Products: NA

Alternate protein names: 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione 4-hydroxylase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MFDDTTPMIERVRAILPRIAANASRAEEMREVPKENIDLLKSTGLHRAFQPKAYGGLEMPLPDFENCIALIATACGSTAW
AFSLLAEHAHQIALYSKQLQDEIWQDDPDTVCSSSIAPYGKAQDVEGGVRFSGEFGWSSGCDHAQWAILGFLRDTPEGGK
VYSFAILPRSDYEIKDTWFTAGMRGTGSKTLVVRDAFVPEHRIETVPALMTLTSAGGGLYPGSTTYHVPFIYVFASCFSA
VSLGIAERMIQLYTERTRNRVRAYTGAKVSQSIPACMRLAESTHQVAAGRAFLEKTWEDMRDHAERRVFPDETRMAFWRT
NQAYAVKMFVAAVDRLFEASGGSSWFDDAEGQRLFRDAHMTAAHAYTDYDICAQILGRALMGLERDPSLF

Sequences:

>Translated_390_residues
MFDDTTPMIERVRAILPRIAANASRAEEMREVPKENIDLLKSTGLHRAFQPKAYGGLEMPLPDFENCIALIATACGSTAW
AFSLLAEHAHQIALYSKQLQDEIWQDDPDTVCSSSIAPYGKAQDVEGGVRFSGEFGWSSGCDHAQWAILGFLRDTPEGGK
VYSFAILPRSDYEIKDTWFTAGMRGTGSKTLVVRDAFVPEHRIETVPALMTLTSAGGGLYPGSTTYHVPFIYVFASCFSA
VSLGIAERMIQLYTERTRNRVRAYTGAKVSQSIPACMRLAESTHQVAAGRAFLEKTWEDMRDHAERRVFPDETRMAFWRT
NQAYAVKMFVAAVDRLFEASGGSSWFDDAEGQRLFRDAHMTAAHAYTDYDICAQILGRALMGLERDPSLF
>Mature_390_residues
MFDDTTPMIERVRAILPRIAANASRAEEMREVPKENIDLLKSTGLHRAFQPKAYGGLEMPLPDFENCIALIATACGSTAW
AFSLLAEHAHQIALYSKQLQDEIWQDDPDTVCSSSIAPYGKAQDVEGGVRFSGEFGWSSGCDHAQWAILGFLRDTPEGGK
VYSFAILPRSDYEIKDTWFTAGMRGTGSKTLVVRDAFVPEHRIETVPALMTLTSAGGGLYPGSTTYHVPFIYVFASCFSA
VSLGIAERMIQLYTERTRNRVRAYTGAKVSQSIPACMRLAESTHQVAAGRAFLEKTWEDMRDHAERRVFPDETRMAFWRT
NQAYAVKMFVAAVDRLFEASGGSSWFDDAEGQRLFRDAHMTAAHAYTDYDICAQILGRALMGLERDPSLF

Specific function: Catalyzes the o-hydroxylation of 3-hydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy- 9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Also catalyzes the o-hydroxylation of a range of

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013107
- InterPro:   IPR006092
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF08028 Acyl-CoA_dh_2; PF02771 Acyl-CoA_dh_N [H]

EC number: NA

Molecular weight: Translated: 43438; Mature: 43438

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDDTTPMIERVRAILPRIAANASRAEEMREVPKENIDLLKSTGLHRAFQPKAYGGLEMP
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
LPDFENCIALIATACGSTAWAFSLLAEHAHQIALYSKQLQDEIWQDDPDTVCSSSIAPYG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
KAQDVEGGVRFSGEFGWSSGCDHAQWAILGFLRDTPEGGKVYSFAILPRSDYEIKDTWFT
CCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEE
AGMRGTGSKTLVVRDAFVPEHRIETVPALMTLTSAGGGLYPGSTTYHVPFIYVFASCFSA
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHH
VSLGIAERMIQLYTERTRNRVRAYTGAKVSQSIPACMRLAESTHQVAAGRAFLEKTWEDM
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDHAERRVFPDETRMAFWRTNQAYAVKMFVAAVDRLFEASGGSSWFDDAEGQRLFRDAHM
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
TAAHAYTDYDICAQILGRALMGLERDPSLF
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFDDTTPMIERVRAILPRIAANASRAEEMREVPKENIDLLKSTGLHRAFQPKAYGGLEMP
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
LPDFENCIALIATACGSTAWAFSLLAEHAHQIALYSKQLQDEIWQDDPDTVCSSSIAPYG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
KAQDVEGGVRFSGEFGWSSGCDHAQWAILGFLRDTPEGGKVYSFAILPRSDYEIKDTWFT
CCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEE
AGMRGTGSKTLVVRDAFVPEHRIETVPALMTLTSAGGGLYPGSTTYHVPFIYVFASCFSA
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHH
VSLGIAERMIQLYTERTRNRVRAYTGAKVSQSIPACMRLAESTHQVAAGRAFLEKTWEDM
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDHAERRVFPDETRMAFWRTNQAYAVKMFVAAVDRLFEASGGSSWFDDAEGQRLFRDAHM
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
TAAHAYTDYDICAQILGRALMGLERDPSLF
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]