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The map label for this gene is manC [H]

Identifier: 77461903

GI number: 77461903

Start: 6368062

End: 6369504

Strand: Direct

Name: manC [H]

Synonym: Pfl01_5683

Alternate gene names: 77461903

Gene position: 6368062-6369504 (Clockwise)

Preceding gene: 77461902

Following gene: 77461904

Centisome position: 98.91

GC content: 63.69

Gene sequence:

>1443_bases
ATGCTGATCCCCGTGATTCTGTCCGGCGGTGCCGGCACCCGTTTGTGGCCGGTGTCCCGGGAGGGCCATCCCAAGCCGTT
CATGACCCTGCCCGACGGCCAGTCGCTGCTGGGCAAGACCTACCGGCGCGCCGCCGCGCTGCTGGACGGCTGGGGCGACA
TTGTCACGGTGACCAACCGCGAGTATTACTTCCAGAGCAAGGATCACTATCAGGCCGCGCACCTGTCCCGCCATCGCGGG
CACTTCCTGCTTGAGCCCACCGGGCGCAACACCGCGCCGGCCATTGCGGCGGCGGCACTGTCGCTGCAAGCCCTGCACGG
TGATGCGGCAATCATGGTGGTGATGCCCGCCGACCATTTGATCGTCAATGAAGAGGCGCTCAAAAGCGCGGTCGAACACG
CGGTCAATCTGGCGAAGGACGGCTATCTGGTGACCTTCGGCGTGGTGCCGACCGCCCCGGAAACCGGTTTCGGTTACATC
GAGACCGGCGCACCGCTCAACGACCGGGGCGCGGCCAAGGTGCAGCGTTTCGTGGAAAAACCCGACCTGCAAACCGCCAC
CCATTACCTGGAAAGCGGCAACTTCCTGTGGAATTCGGGGATGTTCTGCTTCTCCACCGCAACGGTGATTGCCGAACTGG
CGCTGCACGCCCCCGAGTTGCTGGAACAGACGCGCGCCTGCATGGCCGCCAGCGAACCGGTGGAAACCGCCGGTTGTCTG
CAACAGGAGTTGTCCACGCAGCTGTTCGCCGAGATCACCGACATCTCCATTGACTACGCCTTGATGGAACGCTCGGAGAA
AGTCGTGGTGGTACCCGCCGGTTTCGACTGGAGCGACATCGGATCGTGGAGCGCGGTCGCCGCGCTGGTGCCGGCCGACA
CCGATAACAACCGAGCCAGCGGCGAGGCGATCTTCATCGACAGCCGCAACAACTTCGTTCAGAGCGAAGGCCGGCTGGTG
GCCGCCGTCGGTGTGGAAGACCTGATCGTCATCGACACCGCCGACGCGGTACTGGTAGCCCACGCCGACCGTGCGCAGGA
TGTGCGGCGGGTGGCCAAGCAGCTCAAGGACAAATCCCACGAAGCCTATCGCCTGCACCGCACGGTCAGCCGTCCGTGGG
GCACCTACACGGTGCTCGAGGAAGGCCCGCGCTTCAAGATCAAACGCATCGTGGTCAAACCCGGCGGCAAGCTGTCGCTG
CAGATGCACCATCACCGCAATGAACACTGGGTGGTGGTCGAAGGCATGGCCAAGGTCACCAACAATGGCAGCGGCACGAC
GCTGGTGGCGAAGAACGAATCGACGTTCATCGCCGCCGGTCACAAGCACCGTCTGGAGAACCCCGGCGTGATCGACCTGG
TGATCATCGAAGTGCAAAGTGGCGAATACCTGGGAGAGGACGACATCGTCCGCTTCGAAGACCAATACGGCAGGACGGTG
TGA

Upstream 100 bases:

>100_bases
GGCCCAGCGCGTGCTGGGCTGGAAACCGAAAACCGACCTCGACACCCTGATCCGCATGATGATGGATGCGGACATGAAAC
GCGTCGCCAAGGAGTAGGCC

Downstream 100 bases:

>100_bases
ATGCTGCTTTCCCTGTACCGCTCGCTGCACGACTATCGGGGTTTCATCCTCGGTAGCGTCAAGCGGGAGTTTCAAGCGCG
GTATCGCAATTCGCTGTTCG

Product: mannose-1-phosphate guanylyltransferase (GDP)

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLIPVILSGGAGTRLWPVSREGHPKPFMTLPDGQSLLGKTYRRAAALLDGWGDIVTVTNREYYFQSKDHYQAAHLSRHRG
HFLLEPTGRNTAPAIAAAALSLQALHGDAAIMVVMPADHLIVNEEALKSAVEHAVNLAKDGYLVTFGVVPTAPETGFGYI
ETGAPLNDRGAAKVQRFVEKPDLQTATHYLESGNFLWNSGMFCFSTATVIAELALHAPELLEQTRACMAASEPVETAGCL
QQELSTQLFAEITDISIDYALMERSEKVVVVPAGFDWSDIGSWSAVAALVPADTDNNRASGEAIFIDSRNNFVQSEGRLV
AAVGVEDLIVIDTADAVLVAHADRAQDVRRVAKQLKDKSHEAYRLHRTVSRPWGTYTVLEEGPRFKIKRIVVKPGGKLSL
QMHHHRNEHWVVVEGMAKVTNNGSGTTLVAKNESTFIAAGHKHRLENPGVIDLVIIEVQSGEYLGEDDIVRFEDQYGRTV

Sequences:

>Translated_480_residues
MLIPVILSGGAGTRLWPVSREGHPKPFMTLPDGQSLLGKTYRRAAALLDGWGDIVTVTNREYYFQSKDHYQAAHLSRHRG
HFLLEPTGRNTAPAIAAAALSLQALHGDAAIMVVMPADHLIVNEEALKSAVEHAVNLAKDGYLVTFGVVPTAPETGFGYI
ETGAPLNDRGAAKVQRFVEKPDLQTATHYLESGNFLWNSGMFCFSTATVIAELALHAPELLEQTRACMAASEPVETAGCL
QQELSTQLFAEITDISIDYALMERSEKVVVVPAGFDWSDIGSWSAVAALVPADTDNNRASGEAIFIDSRNNFVQSEGRLV
AAVGVEDLIVIDTADAVLVAHADRAQDVRRVAKQLKDKSHEAYRLHRTVSRPWGTYTVLEEGPRFKIKRIVVKPGGKLSL
QMHHHRNEHWVVVEGMAKVTNNGSGTTLVAKNESTFIAAGHKHRLENPGVIDLVIIEVQSGEYLGEDDIVRFEDQYGRTV
>Mature_480_residues
MLIPVILSGGAGTRLWPVSREGHPKPFMTLPDGQSLLGKTYRRAAALLDGWGDIVTVTNREYYFQSKDHYQAAHLSRHRG
HFLLEPTGRNTAPAIAAAALSLQALHGDAAIMVVMPADHLIVNEEALKSAVEHAVNLAKDGYLVTFGVVPTAPETGFGYI
ETGAPLNDRGAAKVQRFVEKPDLQTATHYLESGNFLWNSGMFCFSTATVIAELALHAPELLEQTRACMAASEPVETAGCL
QQELSTQLFAEITDISIDYALMERSEKVVVVPAGFDWSDIGSWSAVAALVPADTDNNRASGEAIFIDSRNNFVQSEGRLV
AAVGVEDLIVIDTADAVLVAHADRAQDVRRVAKQLKDKSHEAYRLHRTVSRPWGTYTVLEEGPRFKIKRIVVKPGGKLSL
QMHHHRNEHWVVVEGMAKVTNNGSGTTLVAKNESTFIAAGHKHRLENPGVIDLVIIEVQSGEYLGEDDIVRFEDQYGRTV

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O9 antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=484, Percent_Identity=44.0082644628099, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 52472; Mature: 52472

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIPVILSGGAGTRLWPVSREGHPKPFMTLPDGQSLLGKTYRRAAALLDGWGDIVTVTNR
CEEEEEEECCCCCEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
EYYFQSKDHYQAAHLSRHRGHFLLEPTGRNTAPAIAAAALSLQALHGDAAIMVVMPADHL
EEEECCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHEEECCCEEEEEEECCCCE
IVNEEALKSAVEHAVNLAKDGYLVTFGVVPTAPETGFGYIETGAPLNDRGAAKVQRFVEK
EECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHC
PDLQTATHYLESGNFLWNSGMFCFSTATVIAELALHAPELLEQTRACMAASEPVETAGCL
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
QQELSTQLFAEITDISIDYALMERSEKVVVVPAGFDWSDIGSWSAVAALVPADTDNNRAS
HHHHHHHHHHHHHHCEEEEHHHCCCCEEEEEECCCCHHHCCCCCEEEEEEECCCCCCCCC
GEAIFIDSRNNFVQSEGRLVAAVGVEDLIVIDTADAVLVAHADRAQDVRRVAKQLKDKSH
CCEEEEECCCCEECCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
EAYRLHRTVSRPWGTYTVLEEGPRFKIKRIVVKPGGKLSLQMHHHRNEHWVVVEGMAKVT
HHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCEEEEEEECCCCCCEEEEEEEEECC
NNGSGTTLVAKNESTFIAAGHKHRLENPGVIDLVIIEVQSGEYLGEDDIVRFEDQYGRTV
CCCCCCEEEEECCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEHHCCCCC
>Mature Secondary Structure
MLIPVILSGGAGTRLWPVSREGHPKPFMTLPDGQSLLGKTYRRAAALLDGWGDIVTVTNR
CEEEEEEECCCCCEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
EYYFQSKDHYQAAHLSRHRGHFLLEPTGRNTAPAIAAAALSLQALHGDAAIMVVMPADHL
EEEECCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHEEECCCEEEEEEECCCCE
IVNEEALKSAVEHAVNLAKDGYLVTFGVVPTAPETGFGYIETGAPLNDRGAAKVQRFVEK
EECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHC
PDLQTATHYLESGNFLWNSGMFCFSTATVIAELALHAPELLEQTRACMAASEPVETAGCL
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHH
QQELSTQLFAEITDISIDYALMERSEKVVVVPAGFDWSDIGSWSAVAALVPADTDNNRAS
HHHHHHHHHHHHHHCEEEEHHHCCCCEEEEEECCCCHHHCCCCCEEEEEEECCCCCCCCC
GEAIFIDSRNNFVQSEGRLVAAVGVEDLIVIDTADAVLVAHADRAQDVRRVAKQLKDKSH
CCEEEEECCCCEECCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
EAYRLHRTVSRPWGTYTVLEEGPRFKIKRIVVKPGGKLSLQMHHHRNEHWVVVEGMAKVT
HHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCEEEEEEECCCCCCEEEEEEEEECC
NNGSGTTLVAKNESTFIAAGHKHRLENPGVIDLVIIEVQSGEYLGEDDIVRFEDQYGRTV
CCCCCCEEEEECCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7515042; 9004408 [H]