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The map label for this gene is potF [H]

Identifier: 77461852

GI number: 77461852

Start: 6307443

End: 6308543

Strand: Direct

Name: potF [H]

Synonym: Pfl01_5632

Alternate gene names: 77461852

Gene position: 6307443-6308543 (Clockwise)

Preceding gene: 77461851

Following gene: 77461854

Centisome position: 97.97

GC content: 61.13

Gene sequence:

>1101_bases
ATGAGCAGACTCAAGCAATTCATCGCGCCGGCGTTGTGCGCGGCGCTGCTGAGCGGCGCCGCACACGCCGAAGAACGCAC
CCTGCGCGTCTACAACTGGTTCGACTACATCACCCCCAAGGCGCTGGAAGATTTCAAGGCGCAGAACAGCCAGACCAAAC
TGGTCTACGACATCTTCGACACCAACGAAGCACTGGAAGCCAAGCTGCTGACCGGCAACTCCGGCTACGACGTGGTGGTG
CCGTCCAACGTGTTCCTCGCCAAGCAGATCGAGGCCGGGGTATTCCAGCCGCTGGATCGCAGCCAGTTGCCGAACTGGAA
CCACCTCGACCCGAAACTGATGAAGCTGATCGAAGCCAACGATCCGGGCAACCAGTTCGCCGTGCCGTACATGTACGGCA
CCATCCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCCGCGCTGGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATC
TTCAAGGAAGAGAACATCAGCAAGCTCAAGCAGTGCGGTGTGGCCCTGCTCGATTCGCCGTCGGAGATCCTGCCGCTGGC
CCTGCAACACCTCGGCCTGGATCCCAACAGCAAGAACCCGGCGGACTACGCCAAGGCTGAAGCGCTGTTGATGAAGATTC
GCCCGTACGTGACCTACTTCCATTCGTCCAAGTACATGGCCGACATCGCCAACGGCGACATCTGCGTCGCGGTCGGTTAT
TCGGGCAGCTTCTCGCAAGCGGCCAACCGCGCCAAGGAAGCGAAGAACGGCGTGGTGGTGGACATGCGCCTGCCGAAAGA
AGGCGCCCCGATCTGGTTCGACATGCTCGCGATCCCGAAAGGCGCGAAGAACCCGCAGGACGCCTACACCTTCATCAACT
ACCTGCTGCAACCGCAGGTGATCGCGCCGGTCAGCGATTTTGTCGGTTATCCGAACCCGAACAAGGACGCCACCGAACTG
GTCGACCCGGCGATTCGCAACAATCCGAATCTGTACCCGACCGATGCGGCGATGAATACGCTGTACACCCTTCAGCCATT
ACCGCGCGATGCGGAACGGGCACGCACGCGGGCGTGGACGAAGATCAAATCCGGCACCTGA

Upstream 100 bases:

>100_bases
TCGCGGCCCTCGGCAAACCGACCCTGTTCGTCATGGAAGGTGGCTACGCGGTGGAAGAAATCGGCATCAACGCGGTGAAC
GTGCTTGAAGGTTTCGAACA

Downstream 100 bases:

>100_bases
AACTTGCTCATCCAACCCGGACCCGCCCCGGCGGGTCTTTGGATACCGACGCTGCGTCTTCACTCACCCCAGGCTTGCCG
CAAACGGGCCAGGGCCGACT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSRLKQFIAPALCAALLSGAAHAEERTLRVYNWFDYITPKALEDFKAQNSQTKLVYDIFDTNEALEAKLLTGNSGYDVVV
PSNVFLAKQIEAGVFQPLDRSQLPNWNHLDPKLMKLIEANDPGNQFAVPYMYGTILIGFNPAKVKAALGDNAPVDSWDLI
FKEENISKLKQCGVALLDSPSEILPLALQHLGLDPNSKNPADYAKAEALLMKIRPYVTYFHSSKYMADIANGDICVAVGY
SGSFSQAANRAKEAKNGVVVDMRLPKEGAPIWFDMLAIPKGAKNPQDAYTFINYLLQPQVIAPVSDFVGYPNPNKDATEL
VDPAIRNNPNLYPTDAAMNTLYTLQPLPRDAERARTRAWTKIKSGT

Sequences:

>Translated_366_residues
MSRLKQFIAPALCAALLSGAAHAEERTLRVYNWFDYITPKALEDFKAQNSQTKLVYDIFDTNEALEAKLLTGNSGYDVVV
PSNVFLAKQIEAGVFQPLDRSQLPNWNHLDPKLMKLIEANDPGNQFAVPYMYGTILIGFNPAKVKAALGDNAPVDSWDLI
FKEENISKLKQCGVALLDSPSEILPLALQHLGLDPNSKNPADYAKAEALLMKIRPYVTYFHSSKYMADIANGDICVAVGY
SGSFSQAANRAKEAKNGVVVDMRLPKEGAPIWFDMLAIPKGAKNPQDAYTFINYLLQPQVIAPVSDFVGYPNPNKDATEL
VDPAIRNNPNLYPTDAAMNTLYTLQPLPRDAERARTRAWTKIKSGT
>Mature_365_residues
SRLKQFIAPALCAALLSGAAHAEERTLRVYNWFDYITPKALEDFKAQNSQTKLVYDIFDTNEALEAKLLTGNSGYDVVVP
SNVFLAKQIEAGVFQPLDRSQLPNWNHLDPKLMKLIEANDPGNQFAVPYMYGTILIGFNPAKVKAALGDNAPVDSWDLIF
KEENISKLKQCGVALLDSPSEILPLALQHLGLDPNSKNPADYAKAEALLMKIRPYVTYFHSSKYMADIANGDICVAVGYS
GSFSQAANRAKEAKNGVVVDMRLPKEGAPIWFDMLAIPKGAKNPQDAYTFINYLLQPQVIAPVSDFVGYPNPNKDATELV
DPAIRNNPNLYPTDAAMNTLYTLQPLPRDAERARTRAWTKIKSGT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=365, Percent_Identity=53.1506849315069, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1787367, Length=316, Percent_Identity=35.4430379746835, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787711, Length=333, Percent_Identity=26.7267267267267, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40442; Mature: 40310

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKQFIAPALCAALLSGAAHAEERTLRVYNWFDYITPKALEDFKAQNSQTKLVYDIFD
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCHHHHHHHHCCCCCCEEEEEEEC
TNEALEAKLLTGNSGYDVVVPSNVFLAKQIEAGVFQPLDRSQLPNWNHLDPKLMKLIEAN
CCCCCEEEEEECCCCCEEEECCCEEEHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHCC
DPGNQFAVPYMYGTILIGFNPAKVKAALGDNAPVDSWDLIFKEENISKLKQCGVALLDSP
CCCCCEEEEEEEEEEEEECCHHHEEEECCCCCCCCCCEEEEECCCHHHHHHCCEEEECCC
SEILPLALQHLGLDPNSKNPADYAKAEALLMKIRPYVTYFHSSKYMADIANGDICVAVGY
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEEECC
SGSFSQAANRAKEAKNGVVVDMRLPKEGAPIWFDMLAIPKGAKNPQDAYTFINYLLQPQV
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
IAPVSDFVGYPNPNKDATELVDPAIRNNPNLYPTDAAMNTLYTLQPLPRDAERARTRAWT
CCCHHHHCCCCCCCCCHHHHHCHHHHCCCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHH
KIKSGT
HCCCCC
>Mature Secondary Structure 
SRLKQFIAPALCAALLSGAAHAEERTLRVYNWFDYITPKALEDFKAQNSQTKLVYDIFD
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCHHHHHHHHCCCCCCEEEEEEEC
TNEALEAKLLTGNSGYDVVVPSNVFLAKQIEAGVFQPLDRSQLPNWNHLDPKLMKLIEAN
CCCCCEEEEEECCCCCEEEECCCEEEHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHCC
DPGNQFAVPYMYGTILIGFNPAKVKAALGDNAPVDSWDLIFKEENISKLKQCGVALLDSP
CCCCCEEEEEEEEEEEEECCHHHEEEECCCCCCCCCCEEEEECCCHHHHHHCCEEEECCC
SEILPLALQHLGLDPNSKNPADYAKAEALLMKIRPYVTYFHSSKYMADIANGDICVAVGY
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEEECC
SGSFSQAANRAKEAKNGVVVDMRLPKEGAPIWFDMLAIPKGAKNPQDAYTFINYLLQPQV
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
IAPVSDFVGYPNPNKDATELVDPAIRNNPNLYPTDAAMNTLYTLQPLPRDAERARTRAWT
CCCHHHHCCCCCCCCCHHHHHCHHHHCCCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHH
KIKSGT
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]