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The map label for this gene is aspA [H]

Identifier: 77461846

GI number: 77461846

Start: 6298662

End: 6300098

Strand: Direct

Name: aspA [H]

Synonym: Pfl01_5625

Alternate gene names: 77461846

Gene position: 6298662-6300098 (Clockwise)

Preceding gene: 77461844

Following gene: 77461847

Centisome position: 97.83

GC content: 61.52

Gene sequence:

>1437_bases
ATGATTTCTATCATGTCCTCCGCTGCATCATTCCGCACAGAAAACGACCTGCTTGGCACCCTCGAAGTCCCTGCTCAAGC
GTATTACGGCATCCAGACCCTGCGAGCGGTGAACAACTTCCGTCTCTCCGGCGTTCCGATTTCGCACTACCCGAAGCTGG
TTGTCGGTCTGGCGATGGTCAAACAGGCTGCCGCTGACGCCAACCGCGAGCTGGGTCACCTGAGCGAAGCCAAGCACGCT
GCCATCAGCGAAGCCTGTGCCCGTCTGATCCGCGGCGATTTCCACGAAGAGTTCGTGGTCGACATGATTCAAGGTGGCGC
CGGTACCTCCACCAACATGAACGCCAACGAAGTGATCGCCAACATCGCGCTGGAAGCGATGGGTCACCAGAAAGGCGAAT
ACCAGTACCTGCACCCGAACGACGACGTGAACATGGCGCAGTCGACCAACGACGCCTATCCGACCGCGATCCGTCTGGGT
CTGCTGCTGGGTCACGACGCGCTGCTGGCCAGCCTCGACAGCCTGATTCAGGCGTTCGCGGCCAAGGGTGAAGAATTCAA
TCACGTCCTGAAGATGGGCCGTACCCAACTGCAAGACGCCGTACCGATGACCCTGGGTCAGGAATTCCGTGCTTTCGCCA
CCACCATGGGCGAAGACCTGGCCCGTCTGAAGACGCTGGCCCCGGAACTGCTGACTGAAGTGAACCTGGGCGGCACCGCG
ATCGGTACCGGCATCAACGCTGACCCGCGTTACCAGGCCCTGGCCGTACAGCGTCTGGCGCTGATCAGCGGTCAACCGCT
GGTTCCAGCCGCCGACCTGATCGAAGCCACCTCCGACATGGGCGCCTTTGTACTGTTCTCTGGCATGCTCAAGCGCACCG
CGGTCAAACTGTCGAAGATCTGCAACGACCTGCGCCTGCTGTCCAGCGGCCCGCGCACCGGCATCAACGAAATCAACCTG
CCGGCGCGTCAGCCAGGCAGCTCGATCATGCCAGGCAAGGTCAACCCGGTAATCCCGGAAGCCGTGAACCAGGTGGCATT
CCAAGTCATCGGTAACGATCTGGCGCTGACCATGGCAGCCGAAGGCGGCCAACTGCAGCTGAACGTGATGGAGCCGCTGA
TCGCCTTCAAGATCCTCGACTCGATCCGCCTGCTGCAACGCGCCATGGACATGCTGCGCGAGCACTGCATCGTCGGCATC
ACCGCCAACGAAGCGCGCTGCCGCGAACTGGTCGAACACTCGATCGGCCTGGTCACCGCACTGAACCCGTACATCGGCTA
CAAAAACGCCACCCGCATCGCCCGTATCGCCCTTGAAAGCGGCCGCGGCGTACTGGAACTGGTGCGCGAAGAAGGTCTGC
TCGACGAAGCCATGCTCGCCGACATCCTGCGCCCGGAAAACATGATTGCTCCGCGTCTGGTGCCTCTGAAGGCCTGA

Upstream 100 bases:

>100_bases
ATGCCGAATGTGCATAGGGCAGTGTTTAACAGCATTGGCCCAAAAATTCCCACAAGCCTAGCATTCGCAGCGTTCCGGCA
CAGACCGGGTCCATATCGAG

Downstream 100 bases:

>100_bases
TCCGACGCGTTACTTGTAGCACCGCTCACCAGGTCGAGGGACTAGACACCTCTCACCTTTTGAGGGCTTGGGGATCGTTC
CCCGAGCCCTTTTTTTTAAC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MISIMSSAASFRTENDLLGTLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMVKQAAADANRELGHLSEAKHA
AISEACARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIANIALEAMGHQKGEYQYLHPNDDVNMAQSTNDAYPTAIRLG
LLLGHDALLASLDSLIQAFAAKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTMGEDLARLKTLAPELLTEVNLGGTA
IGTGINADPRYQALAVQRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDLRLLSSGPRTGINEINL
PARQPGSSIMPGKVNPVIPEAVNQVAFQVIGNDLALTMAAEGGQLQLNVMEPLIAFKILDSIRLLQRAMDMLREHCIVGI
TANEARCRELVEHSIGLVTALNPYIGYKNATRIARIALESGRGVLELVREEGLLDEAMLADILRPENMIAPRLVPLKA

Sequences:

>Translated_478_residues
MISIMSSAASFRTENDLLGTLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMVKQAAADANRELGHLSEAKHA
AISEACARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIANIALEAMGHQKGEYQYLHPNDDVNMAQSTNDAYPTAIRLG
LLLGHDALLASLDSLIQAFAAKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTMGEDLARLKTLAPELLTEVNLGGTA
IGTGINADPRYQALAVQRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDLRLLSSGPRTGINEINL
PARQPGSSIMPGKVNPVIPEAVNQVAFQVIGNDLALTMAAEGGQLQLNVMEPLIAFKILDSIRLLQRAMDMLREHCIVGI
TANEARCRELVEHSIGLVTALNPYIGYKNATRIARIALESGRGVLELVREEGLLDEAMLADILRPENMIAPRLVPLKA
>Mature_478_residues
MISIMSSAASFRTENDLLGTLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMVKQAAADANRELGHLSEAKHA
AISEACARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIANIALEAMGHQKGEYQYLHPNDDVNMAQSTNDAYPTAIRLG
LLLGHDALLASLDSLIQAFAAKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTMGEDLARLKTLAPELLTEVNLGGTA
IGTGINADPRYQALAVQRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDLRLLSSGPRTGINEINL
PARQPGSSIMPGKVNPVIPEAVNQVAFQVIGNDLALTMAAEGGQLQLNVMEPLIAFKILDSIRLLQRAMDMLREHCIVGI
TANEARCRELVEHSIGLVTALNPYIGYKNATRIARIALESGRGVLELVREEGLLDEAMLADILRPENMIAPRLVPLKA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=469, Percent_Identity=41.1513859275053, Blast_Score=356, Evalue=3e-98,
Organism=Escherichia coli, GI87082375, Length=463, Percent_Identity=57.8833693304536, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=42.1166306695464, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17553882, Length=468, Percent_Identity=42.0940170940171, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565146, Length=331, Percent_Identity=41.6918429003021, Blast_Score=265, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324993, Length=469, Percent_Identity=41.36460554371, Blast_Score=349, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24640177, Length=473, Percent_Identity=41.014799154334, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24640179, Length=469, Percent_Identity=41.1513859275053, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=40.301724137931, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI78710009, Length=466, Percent_Identity=39.2703862660944, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24583245, Length=466, Percent_Identity=36.9098712446352, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51486; Mature: 51486

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIMSSAASFRTENDLLGTLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMV
CCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHH
KQAAADANRELGHLSEAKHAAISEACARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
NIALEAMGHQKGEYQYLHPNDDVNMAQSTNDAYPTAIRLGLLLGHDALLASLDSLIQAFA
HHHHHHHCCCCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTMGEDLARLKTLAPELLTEVNLGGTA
HCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IGTGINADPRYQALAVQRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKI
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
CNDLRLLSSGPRTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFQVIGNDLALTMAA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEC
EGGQLQLNVMEPLIAFKILDSIRLLQRAMDMLREHCIVGITANEARCRELVEHSIGLVTA
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCHHHH
LNPYIGYKNATRIARIALESGRGVLELVREEGLLDEAMLADILRPENMIAPRLVPLKA
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MISIMSSAASFRTENDLLGTLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMV
CCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHH
KQAAADANRELGHLSEAKHAAISEACARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
NIALEAMGHQKGEYQYLHPNDDVNMAQSTNDAYPTAIRLGLLLGHDALLASLDSLIQAFA
HHHHHHHCCCCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTMGEDLARLKTLAPELLTEVNLGGTA
HCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IGTGINADPRYQALAVQRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKI
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
CNDLRLLSSGPRTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFQVIGNDLALTMAA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEC
EGGQLQLNVMEPLIAFKILDSIRLLQRAMDMLREHCIVGITANEARCRELVEHSIGLVTA
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCHHHH
LNPYIGYKNATRIARIALESGRGVLELVREEGLLDEAMLADILRPENMIAPRLVPLKA
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]