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The map label for this gene is potF [H]

Identifier: 77461625

GI number: 77461625

Start: 6066945

End: 6068072

Strand: Reverse

Name: potF [H]

Synonym: Pfl01_5404

Alternate gene names: 77461625

Gene position: 6068072-6066945 (Counterclockwise)

Preceding gene: 77461626

Following gene: 77461624

Centisome position: 94.25

GC content: 58.07

Gene sequence:

>1128_bases
TTGGGCCCAACTAAATCAGAGGACCTCCACTTGCCTATTTTTTCTTCTTTGCGCAATGCCCTGCTGGCCGCTGCCGGCCT
GACGTTCGCTGTCGGTGCCCAAGCCGCCGGTACTGTGCATATTTATAACTGGTCGGATTACATCGGCGAGACCACCCTGG
CCGATTTCCAGAAAGAAACCGGGATCAAACCGGTCTACGACGTGTTCGATTCCAACGAAACCCTGGAAGGCAAACTGCTC
GCCGGGCGTACCGGCTACGACGTGGTCGTGCCGTCGAACCACTTCCTCGGCAAGCAGATCAAGGCGGGCGCGTTCCAGAA
GCTCGACAAGGCGCAACTGCCGAACTATTCCAACCTCGATCCGGTGCTGCTCAAGCGCCTGGAACAGAACGATCCGGGCA
ACCTGTACGCCGTACCGTACCTGTGGGGCACCAACGGCATCGGCTACAACGTCGATAAAGTGAAGGCCGTGCTCGGCGTC
GACAAGATCGACTCCTGGAGCGTGCTTTTCGAGCCGGAGAACATCAAGAAGCTGCACAGCTGCGGCGTGGCGTTCCTCGA
TTCGGCCGATGAAATGATGCCGACCGTGCTCAACTACATGGGCCTGAACGCCAACAGCACCAATCCCAAGGATTACGAGA
AGGCCACGGCCAAACTGCTGGCGGTGCGTCCGTACGTGACCTACTTCCACTCCTCGAAATACATCGGCGATCTGGCCAAC
GGCGACATCTGCGTGGCCATCGGTTTCTCCGGCGACATCTTCCAGGCCAAGAATCGCGCCGAAGAAGCCAAGAAGGGCGT
GAACATCGCCTACTCGATCCCGAAAGAGGGCGGCGCGCTGTGGTTCGACATGCTGGCGATTCCGAAGGATTCGGCCAACG
TCAAAGAGGCCCATGCCTTCATCAACTATTTGCTGAAACCTGAGGTGATCGCCCAGGTCAGTGATTACGTCGGTTACGCC
AACCCCAATCCGGGTTCGGACAAGCTGATGGAGCAGTCCATCCGCACCGACGAGTCGGTTTATCCACCGCAGGCCGTGCT
CGACAAGACCTACGTGTCGATCGAGTTGCCGCCGAACATTCAGCGTTTGATGACCCGCAGCTGGACCAAGGTCAAGTCGG
GTAAATAG

Upstream 100 bases:

>100_bases
ATTAAATGACAGAAAGTTTTGCTGGAACGGTTTTTCGAAGGTAAGTTGCGCGCCGGTTTTGTTGCCGGGCAACCGTGACT
GTCATGCAGCACGGGGCAAC

Downstream 100 bases:

>100_bases
CTTCAACGTACAAACTATCCTGGGTTCGTCTTGCGTGGGCGAACTGCACTCTTTTTTTCTGGGAGTTTTGTAAATGGCAG
TTGCCTCCGGCGCCTATAAG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MGPTKSEDLHLPIFSSLRNALLAAAGLTFAVGAQAAGTVHIYNWSDYIGETTLADFQKETGIKPVYDVFDSNETLEGKLL
AGRTGYDVVVPSNHFLGKQIKAGAFQKLDKAQLPNYSNLDPVLLKRLEQNDPGNLYAVPYLWGTNGIGYNVDKVKAVLGV
DKIDSWSVLFEPENIKKLHSCGVAFLDSADEMMPTVLNYMGLNANSTNPKDYEKATAKLLAVRPYVTYFHSSKYIGDLAN
GDICVAIGFSGDIFQAKNRAEEAKKGVNIAYSIPKEGGALWFDMLAIPKDSANVKEAHAFINYLLKPEVIAQVSDYVGYA
NPNPGSDKLMEQSIRTDESVYPPQAVLDKTYVSIELPPNIQRLMTRSWTKVKSGK

Sequences:

>Translated_375_residues
MGPTKSEDLHLPIFSSLRNALLAAAGLTFAVGAQAAGTVHIYNWSDYIGETTLADFQKETGIKPVYDVFDSNETLEGKLL
AGRTGYDVVVPSNHFLGKQIKAGAFQKLDKAQLPNYSNLDPVLLKRLEQNDPGNLYAVPYLWGTNGIGYNVDKVKAVLGV
DKIDSWSVLFEPENIKKLHSCGVAFLDSADEMMPTVLNYMGLNANSTNPKDYEKATAKLLAVRPYVTYFHSSKYIGDLAN
GDICVAIGFSGDIFQAKNRAEEAKKGVNIAYSIPKEGGALWFDMLAIPKDSANVKEAHAFINYLLKPEVIAQVSDYVGYA
NPNPGSDKLMEQSIRTDESVYPPQAVLDKTYVSIELPPNIQRLMTRSWTKVKSGK
>Mature_374_residues
GPTKSEDLHLPIFSSLRNALLAAAGLTFAVGAQAAGTVHIYNWSDYIGETTLADFQKETGIKPVYDVFDSNETLEGKLLA
GRTGYDVVVPSNHFLGKQIKAGAFQKLDKAQLPNYSNLDPVLLKRLEQNDPGNLYAVPYLWGTNGIGYNVDKVKAVLGVD
KIDSWSVLFEPENIKKLHSCGVAFLDSADEMMPTVLNYMGLNANSTNPKDYEKATAKLLAVRPYVTYFHSSKYIGDLANG
DICVAIGFSGDIFQAKNRAEEAKKGVNIAYSIPKEGGALWFDMLAIPKDSANVKEAHAFINYLLKPEVIAQVSDYVGYAN
PNPGSDKLMEQSIRTDESVYPPQAVLDKTYVSIELPPNIQRLMTRSWTKVKSGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=361, Percent_Identity=56.5096952908587, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1787367, Length=314, Percent_Identity=36.3057324840764, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787711, Length=300, Percent_Identity=24.6666666666667, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41171; Mature: 41040

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTKSEDLHLPIFSSLRNALLAAAGLTFAVGAQAAGTVHIYNWSDYIGETTLADFQKET
CCCCCCCCEECHHHHHHHHHHHHHHCCHHEECCCCCCEEEEEECHHHHCCHHHHHHHHHC
GIKPVYDVFDSNETLEGKLLAGRTGYDVVVPSNHFLGKQIKAGAFQKLDKAQLPNYSNLD
CCCHHHHHCCCCCCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
PVLLKRLEQNDPGNLYAVPYLWGTNGIGYNVDKVKAVLGVDKIDSWSVLFEPENIKKLHS
HHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHH
CGVAFLDSADEMMPTVLNYMGLNANSTNPKDYEKATAKLLAVRPYVTYFHSSKYIGDLAN
CCCHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECHHHHHHCCCCCCCCCCC
GDICVAIGFSGDIFQAKNRAEEAKKGVNIAYSIPKEGGALWFDMLAIPKDSANVKEAHAF
CCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHH
INYLLKPEVIAQVSDYVGYANPNPGSDKLMEQSIRTDESVYPPQAVLDKTYVSIELPPNI
HHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCEEEEEECCCCH
QRLMTRSWTKVKSGK
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
GPTKSEDLHLPIFSSLRNALLAAAGLTFAVGAQAAGTVHIYNWSDYIGETTLADFQKET
CCCCCCCEECHHHHHHHHHHHHHHCCHHEECCCCCCEEEEEECHHHHCCHHHHHHHHHC
GIKPVYDVFDSNETLEGKLLAGRTGYDVVVPSNHFLGKQIKAGAFQKLDKAQLPNYSNLD
CCCHHHHHCCCCCCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
PVLLKRLEQNDPGNLYAVPYLWGTNGIGYNVDKVKAVLGVDKIDSWSVLFEPENIKKLHS
HHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHH
CGVAFLDSADEMMPTVLNYMGLNANSTNPKDYEKATAKLLAVRPYVTYFHSSKYIGDLAN
CCCHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECHHHHHHCCCCCCCCCCC
GDICVAIGFSGDIFQAKNRAEEAKKGVNIAYSIPKEGGALWFDMLAIPKDSANVKEAHAF
CCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHH
INYLLKPEVIAQVSDYVGYANPNPGSDKLMEQSIRTDESVYPPQAVLDKTYVSIELPPNI
HHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCEEEEEECCCCH
QRLMTRSWTKVKSGK
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]