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The map label for this gene is thyA [H]

Identifier: 77461594

GI number: 77461594

Start: 6035683

End: 6036654

Strand: Reverse

Name: thyA [H]

Synonym: Pfl01_5373

Alternate gene names: 77461594

Gene position: 6036654-6035683 (Counterclockwise)

Preceding gene: 77461595

Following gene: 77461592

Centisome position: 93.76

GC content: 60.49

Gene sequence:

>972_bases
ATGAAGCAATATCTCGATCTGGTGTCGCACGTCATCAACAACGGCACCAAACAGGCCAACCGTACCGGCGTGAACACCAT
CAGCTTTCCGGGCGCCATGTTGCGCTTCGACCTGCAGGAAGGTTTCCCGGCGATCACCACCCGCAAGATGGCCTTCAAAT
CGGCCATCGGCGAGATGTGCGGCTTTCTGCGTGGCGTGAACAACGCCGCCGAATTCCGTGCGCTGGGCTGCAAGGTCTGG
GATCAGAACGCCAACGAAAACGCCCAGTGGCTGGCCAACCCGTTCCGCCAGGGCGAAGACGACCTCGGCGAAATCTACGG
CGTGCAATGGCGCAAATGGCCGGCGTACAAGCAGATCCCGCTGAGCAACACCGCGGCCATCGAACAAACCTTGAGCAACG
GCTACAAGCAGATTGCCCAGGGCGAAGAAGACGGCCAGGCCTACGTGGTCTTGTACAAAGCCATCGATCAAGTGCGCCAG
TGCGTCGATACGATCATCAAGGACCCGGGCAGCCGCCGCATCCTGTTCCACGGCTGGAACTGCGCCCAGCTCGATGAAAT
GGCCCTGCCGCCGTGCCATCTGCTGTACCAGTTCCACCCGAATGTCGAGACCAAAGAGATCTCCCTGACCCTCTACATCC
GTTCCAACGACCTGGGCCTGGGCACGCCGTTCAATCTCACCGAAGGCGCCGCGCTGCTGAGCCTGATCGGTCGCCTGACC
GGCTACACGCCGCGCTGGTTCACCTATTTCATCGGTGACGCCCACGTCTACGAAAACCACCTGGACATGCTCAACGAACA
GCTCAAGCGCGAGCCGTTCGCCATGCCGAAACTGAAGATTTCGGATCGCGTGCCGGAGTTTGCCAAGACCGGTGTTTATC
AGCCGGAATGGCTGGAACTGGTGGAGCCGAGTGACTTCTCGCTGGAAGGCTACGAGCACCATGCGCCGATGACCGCGCCG
ATGGCGGTCTGA

Upstream 100 bases:

>100_bases
GGCTGGCCTACCACCGCGCCCCGGCGGCGCCGGTTGCACCGACAGCTTAAATATTCGAACCCCGGCGACGTTTGCCGGGG
CTCAAGGACACAGGTAACTC

Downstream 100 bases:

>100_bases
AGCACGAGTGACAAACAACGGAGGGAGCCAAATGGCTCCCTCCGTTGTTTCTGGCGCTTAATGACCGTGGCTTCTGCCCA
CATGCGAATGCTCAACCTCC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQYLDLVSHVINNGTKQANRTGVNTISFPGAMLRFDLQEGFPAITTRKMAFKSAIGEMCGFLRGVNNAAEFRALGCKVW
DQNANENAQWLANPFRQGEDDLGEIYGVQWRKWPAYKQIPLSNTAAIEQTLSNGYKQIAQGEEDGQAYVVLYKAIDQVRQ
CVDTIIKDPGSRRILFHGWNCAQLDEMALPPCHLLYQFHPNVETKEISLTLYIRSNDLGLGTPFNLTEGAALLSLIGRLT
GYTPRWFTYFIGDAHVYENHLDMLNEQLKREPFAMPKLKISDRVPEFAKTGVYQPEWLELVEPSDFSLEGYEHHAPMTAP
MAV

Sequences:

>Translated_323_residues
MKQYLDLVSHVINNGTKQANRTGVNTISFPGAMLRFDLQEGFPAITTRKMAFKSAIGEMCGFLRGVNNAAEFRALGCKVW
DQNANENAQWLANPFRQGEDDLGEIYGVQWRKWPAYKQIPLSNTAAIEQTLSNGYKQIAQGEEDGQAYVVLYKAIDQVRQ
CVDTIIKDPGSRRILFHGWNCAQLDEMALPPCHLLYQFHPNVETKEISLTLYIRSNDLGLGTPFNLTEGAALLSLIGRLT
GYTPRWFTYFIGDAHVYENHLDMLNEQLKREPFAMPKLKISDRVPEFAKTGVYQPEWLELVEPSDFSLEGYEHHAPMTAP
MAV
>Mature_323_residues
MKQYLDLVSHVINNGTKQANRTGVNTISFPGAMLRFDLQEGFPAITTRKMAFKSAIGEMCGFLRGVNNAAEFRALGCKVW
DQNANENAQWLANPFRQGEDDLGEIYGVQWRKWPAYKQIPLSNTAAIEQTLSNGYKQIAQGEEDGQAYVVLYKAIDQVRQ
CVDTIIKDPGSRRILFHGWNCAQLDEMALPPCHLLYQFHPNVETKEISLTLYIRSNDLGLGTPFNLTEGAALLSLIGRLT
GYTPRWFTYFIGDAHVYENHLDMLNEQLKREPFAMPKLKISDRVPEFAKTGVYQPEWLELVEPSDFSLEGYEHHAPMTAP
MAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=322, Percent_Identity=35.0931677018634, Blast_Score=197, Evalue=8e-51,
Organism=Escherichia coli, GI1789191, Length=323, Percent_Identity=34.6749226006192, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71993377, Length=323, Percent_Identity=30.9597523219814, Blast_Score=167, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6324648, Length=329, Percent_Identity=28.2674772036474, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17137556, Length=321, Percent_Identity=34.8909657320872, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36568; Mature: 36568

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLVSHVINNGTKQANRTGVNTISFPGAMLRFDLQEGFPAITTRKMAFKSAIGEMC
CHHHHHHHHHHHCCCCCHHCCCCCCEEECCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHH
GFLRGVNNAAEFRALGCKVWDQNANENAQWLANPFRQGEDDLGEIYGVQWRKWPAYKQIP
HHHHCCCCHHHHHHHCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
LSNTAAIEQTLSNGYKQIAQGEEDGQAYVVLYKAIDQVRQCVDTIIKDPGSRRILFHGWN
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
CAQLDEMALPPCHLLYQFHPNVETKEISLTLYIRSNDLGLGTPFNLTEGAALLSLIGRLT
HHHHHHHCCCHHHHHEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHVYENHLDMLNEQLKREPFAMPKLKISDRVPEFAKTGVYQPEWLEL
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCHHHHCCCCCCHHHHH
VEPSDFSLEGYEHHAPMTAPMAV
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLVSHVINNGTKQANRTGVNTISFPGAMLRFDLQEGFPAITTRKMAFKSAIGEMC
CHHHHHHHHHHHCCCCCHHCCCCCCEEECCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHH
GFLRGVNNAAEFRALGCKVWDQNANENAQWLANPFRQGEDDLGEIYGVQWRKWPAYKQIP
HHHHCCCCHHHHHHHCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
LSNTAAIEQTLSNGYKQIAQGEEDGQAYVVLYKAIDQVRQCVDTIIKDPGSRRILFHGWN
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
CAQLDEMALPPCHLLYQFHPNVETKEISLTLYIRSNDLGLGTPFNLTEGAALLSLIGRLT
HHHHHHHCCCHHHHHEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHVYENHLDMLNEQLKREPFAMPKLKISDRVPEFAKTGVYQPEWLEL
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCHHHHCCCCCCHHHHH
VEPSDFSLEGYEHHAPMTAPMAV
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]