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The map label for this gene is dhfrIII [H]

Identifier: 77461582

GI number: 77461582

Start: 6022221

End: 6022733

Strand: Reverse

Name: dhfrIII [H]

Synonym: Pfl01_5361

Alternate gene names: 77461582

Gene position: 6022733-6022221 (Counterclockwise)

Preceding gene: 77461585

Following gene: 77461580

Centisome position: 93.54

GC content: 60.82

Gene sequence:

>513_bases
ATGACTAAATCACTCCCCCTCAGCCTGATTGCAGCCCTCGGTGAAAACCGTGTGATCGGCGTCGACAACAGCATGCCCTG
GCACCTGCCGGGGGACTTCAAATACTTCAAGGCCACGACGCTCGGCAAGCCGATCATCATGGGCCGCAAGACCTGGGACT
CGCTGGGTCGCCCGTTGCCGGGCCGTCTGAATATCGTGGTCAGCCGTCAGGCCGGTCTGGTGCTGGAGGGCGCGGAAGTC
TATCCGTCGCTGGAAGCGGCCGTGGTTCGCGCCGAAGAATGGGCGAAAGATCAGGGCGTCGATGAGTTGATGCTGATTGG
CGGGGCGCAGTTGTATGCGCAGGGGCTGGCGCAGGCGGATCGTCTGTATCTGACCCGTGTGGCACTGAGCCCGGAAGGCG
ATGCGTGGTTTCCGGAGTTCGATCTGAACCAGTGGAAGCTGGTGTCGAACGTGCCGAATCCGGCTGAAGGCGACAAACCG
GCTTACAATTTCGAAGTCTGGGAAAAAGCCTGA

Upstream 100 bases:

>100_bases
AGTCAGTTCAGTCACGCGGCATCCCGTTGCTTGATTGAGCCGTGAGCATAACCGCTGTGGCGCTTATCGGGGTAAGGGCG
GCTCTGGTATCCTCGCCGGC

Downstream 100 bases:

>100_bases
ACCTGGGGCGAGGGAGCACGCTCCCTCGCCACAACGTCAATCAAGCCTGAGCAAGCTCCGCATGCTCATCGGCATCCAGC
AGTGCCTTGTCGGTCTGCTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MTKSLPLSLIAALGENRVIGVDNSMPWHLPGDFKYFKATTLGKPIIMGRKTWDSLGRPLPGRLNIVVSRQAGLVLEGAEV
YPSLEAAVVRAEEWAKDQGVDELMLIGGAQLYAQGLAQADRLYLTRVALSPEGDAWFPEFDLNQWKLVSNVPNPAEGDKP
AYNFEVWEKA

Sequences:

>Translated_170_residues
MTKSLPLSLIAALGENRVIGVDNSMPWHLPGDFKYFKATTLGKPIIMGRKTWDSLGRPLPGRLNIVVSRQAGLVLEGAEV
YPSLEAAVVRAEEWAKDQGVDELMLIGGAQLYAQGLAQADRLYLTRVALSPEGDAWFPEFDLNQWKLVSNVPNPAEGDKP
AYNFEVWEKA
>Mature_169_residues
TKSLPLSLIAALGENRVIGVDNSMPWHLPGDFKYFKATTLGKPIIMGRKTWDSLGRPLPGRLNIVVSRQAGLVLEGAEVY
PSLEAAVVRAEEWAKDQGVDELMLIGGAQLYAQGLAQADRLYLTRVALSPEGDAWFPEFDLNQWKLVSNVPNPAEGDKPA
YNFEVWEKA

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=181, Percent_Identity=34.2541436464088, Blast_Score=91, Evalue=5e-19,
Organism=Homo sapiens, GI28827793, Length=183, Percent_Identity=33.879781420765, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1786233, Length=164, Percent_Identity=42.6829268292683, Blast_Score=134, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17505899, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324810, Length=136, Percent_Identity=31.6176470588235, Blast_Score=68, Evalue=8e-13,
Organism=Drosophila melanogaster, GI116008026, Length=148, Percent_Identity=33.7837837837838, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647458, Length=148, Percent_Identity=33.7837837837838, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18752; Mature: 18620

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSLPLSLIAALGENRVIGVDNSMPWHLPGDFKYFKATTLGKPIIMGRKTWDSLGRPLP
CCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHHCCCCC
GRLNIVVSRQAGLVLEGAEVYPSLEAAVVRAEEWAKDQGVDELMLIGGAQLYAQGLAQAD
CCEEEEEECCCCEEEECHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
RLYLTRVALSPEGDAWFPEFDLNQWKLVSNVPNPAEGDKPAYNFEVWEKA
HEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
TKSLPLSLIAALGENRVIGVDNSMPWHLPGDFKYFKATTLGKPIIMGRKTWDSLGRPLP
CCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHHCCCCC
GRLNIVVSRQAGLVLEGAEVYPSLEAAVVRAEEWAKDQGVDELMLIGGAQLYAQGLAQAD
CCEEEEEECCCCEEEECHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
RLYLTRVALSPEGDAWFPEFDLNQWKLVSNVPNPAEGDKPAYNFEVWEKA
HEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]