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The map label for this gene is pilJ [H]

Identifier: 77461526

GI number: 77461526

Start: 5970928

End: 5972988

Strand: Reverse

Name: pilJ [H]

Synonym: Pfl01_5305

Alternate gene names: 77461526

Gene position: 5972988-5970928 (Counterclockwise)

Preceding gene: 77461527

Following gene: 77461525

Centisome position: 92.77

GC content: 63.85

Gene sequence:

>2061_bases
ATGATCAAAGCAAAAACAGGCAAGCCCGCAGAAGGCTCGCGCAGCCGCTCGCAGATCATCGTGCTGTTCATCGCACTGAT
CGTGTTCATCATGCTGCTGTTCGCCAACTTCGCTTACCTCAACACCCAGGCCAACTACGACAAGCAGTACATCGGCCACG
CCGGCGAGCTGCGGGTGCTGTCGCAGCGGATCGCCAAGAACGCCACCGAAGCCGCCGCCGGCAAGGCTGCCGCGTTCAAG
CTGCTCAGCGATGCGCGCAACGATTTTGCCCAGCGCTGGGGCTACCTGAAGAAGGGCGACCCGGCCACCGGCCTGCCGCC
GGCGCCGTCCGTCGTGCGCCCGGAAATGCGCGCCGTGCAGCTGGACTGGGAACGCCTGCTGAAAAACACCGACGCGATTC
TCTCCAGCGAACAGACCGTGCTGTCCCTGCATCAGGTGGCCGCGACCCTGGCCGAAACCGTGCCGCAGTTGCAGATCGAA
TACGAAAAAGTCGTCGAGATTCTCCTGCAACGCGGTGCCCCGGCGGCGCAGGTGGCGATGGCCCAGCGTCAGTCGCTGCT
GGCCGAACGGATTCTCGGTGCGGTGAACACCGTGCTGTCGGGCGATGAAAACTCCCAGCAGGCCGCCGACGCCTTCGGTC
GCGATGCCGCGCGCTTCGGTCAGGTGCTCAATGGCATGTTGCAAGGCAACCCGGCGCTGAAAGTCAGCCAGGTCGAAGAC
CGCGACGCCCGTGCGCGTCTGAGCGAGATTTCCGAGCTGTTCCAGTTCGTGTCCGGTTCGGTGGATGAAATCCTAGAAAC
CTCGCCGGAGCTGTTCAAGGTTCGTGAGTCGGCCACCAACATCTTCAGCCTGTCGCAGACCCTGCTCGACGAAGCCTCGC
ACCTGGCCAGCCGTTTCGAAAACCTGGCAGGCGGGCGCAGCACCGACACCATCGGCGGTTATGTGCTGGGCCTGCTGGCG
CTGGCCTCGATCATTCTGATCGGCATGGTCATGGTCCGGGAAACCAACCGTCAACTGCGCGAGACCGCCGAGAAAAACGA
GCGCAACCAGAACGCGATCATGCGCCTGCTCGACGAGATCGAAGACCTCGCCGACGGCGACCTGACCGTGACTGCCTCGG
TGACCGAAGACTTCACCGGAACCATCGCCGACTCGATCAACTATTCAGTCGACCAGTTGCGCGATCTGGTCGCCACCATC
AACCTCACTGCCGGACAGGTCGCCGCTGCGGTGCAGGAAACCCAGGCCACCGCCATGCACCTGGCCCAAGCCTCGGAGCA
TCAGGCCCAGCAGATTTCCGAAGCCTCGACCGCGATCAGCGACATGGCCCAATCCATCGATCAGGTGTCGGCCAACGCCG
CCGAATCCTCGGCAGTGGCCGAGCGTTCGGTGGAGATCGCCAACAAGGGCAACGAGGTGGTGCACAACACCATCCACGGT
ATGGACAACATCCGCGAACAGATCCAGGACACCGCCAAGCGCATCAAGCGCCTGGGCGAGTCGTCCCAGGAAATCGGCGA
CATCGTCAGCCTGATCGATGACATTGCCGACCAGACCAACATCCTCGCCCTCAACGCGGCGATTCAGGCGTCGATGGCCG
GTGATGCCGGGCGCGGTTTCGCGGTTGTGGCCGACGAAGTACAGCGGCTGGCCGAGCGTTCGTCCGCCGCGACCCGGCAG
ATCGAGACGCTGGTGCGGGCGATCCAGACCGACACCAACGAAGCCGTGATTTCCATGGAGCAGACCACCACCGAAGTGGT
GCGCGGCGCGCGGCTGGCGCAGGATGCGGGTGTGGCCCTGGAAGAAATCGAAGGCGTGTCCAAGACCCTCGCGGCGCTGA
TCCAGAGCATTTCCAACGCGGCGCAGCAGCAGACGTCTTCGGCCGGGCAGATTTCCCTGACGATGAACGTGATCCAGCAG
ATCACCTCGCAGACCTCGTCCGGTTCCACGGCCACCGCCGAGAGCATCGGCAACCTGGCGAAAATGGCCAGCCAGTTGCG
TCGCTCGGTGTCCGGGTTCACCTTGCCGACGACGCCTGCGCCCGTCGCGGACAAGGCGTGA

Upstream 100 bases:

>100_bases
GGGTATTTCCGGCGCGAGCAGAACTGGCGGGTGTTCAGCCTGTTTGCGCTGGCGCAGTCACCGAGTTTCATGCACGTCGC
GGTTTAACAGGCGAGGACCG

Downstream 100 bases:

>100_bases
GGCCTGCGGGCGAGTTCATGTCGAGCGGAGTGGTTATGGGTGACCGGCACGACTACGTGGCCCTCGAATGGGTCAAGGGT
GAAATCGCCGAGACGCTGAG

Product: chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MIKAKTGKPAEGSRSRSQIIVLFIALIVFIMLLFANFAYLNTQANYDKQYIGHAGELRVLSQRIAKNATEAAAGKAAAFK
LLSDARNDFAQRWGYLKKGDPATGLPPAPSVVRPEMRAVQLDWERLLKNTDAILSSEQTVLSLHQVAATLAETVPQLQIE
YEKVVEILLQRGAPAAQVAMAQRQSLLAERILGAVNTVLSGDENSQQAADAFGRDAARFGQVLNGMLQGNPALKVSQVED
RDARARLSEISELFQFVSGSVDEILETSPELFKVRESATNIFSLSQTLLDEASHLASRFENLAGGRSTDTIGGYVLGLLA
LASIILIGMVMVRETNRQLRETAEKNERNQNAIMRLLDEIEDLADGDLTVTASVTEDFTGTIADSINYSVDQLRDLVATI
NLTAGQVAAAVQETQATAMHLAQASEHQAQQISEASTAISDMAQSIDQVSANAAESSAVAERSVEIANKGNEVVHNTIHG
MDNIREQIQDTAKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSAATRQ
IETLVRAIQTDTNEAVISMEQTTTEVVRGARLAQDAGVALEEIEGVSKTLAALIQSISNAAQQQTSSAGQISLTMNVIQQ
ITSQTSSGSTATAESIGNLAKMASQLRRSVSGFTLPTTPAPVADKA

Sequences:

>Translated_686_residues
MIKAKTGKPAEGSRSRSQIIVLFIALIVFIMLLFANFAYLNTQANYDKQYIGHAGELRVLSQRIAKNATEAAAGKAAAFK
LLSDARNDFAQRWGYLKKGDPATGLPPAPSVVRPEMRAVQLDWERLLKNTDAILSSEQTVLSLHQVAATLAETVPQLQIE
YEKVVEILLQRGAPAAQVAMAQRQSLLAERILGAVNTVLSGDENSQQAADAFGRDAARFGQVLNGMLQGNPALKVSQVED
RDARARLSEISELFQFVSGSVDEILETSPELFKVRESATNIFSLSQTLLDEASHLASRFENLAGGRSTDTIGGYVLGLLA
LASIILIGMVMVRETNRQLRETAEKNERNQNAIMRLLDEIEDLADGDLTVTASVTEDFTGTIADSINYSVDQLRDLVATI
NLTAGQVAAAVQETQATAMHLAQASEHQAQQISEASTAISDMAQSIDQVSANAAESSAVAERSVEIANKGNEVVHNTIHG
MDNIREQIQDTAKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSAATRQ
IETLVRAIQTDTNEAVISMEQTTTEVVRGARLAQDAGVALEEIEGVSKTLAALIQSISNAAQQQTSSAGQISLTMNVIQQ
ITSQTSSGSTATAESIGNLAKMASQLRRSVSGFTLPTTPAPVADKA
>Mature_686_residues
MIKAKTGKPAEGSRSRSQIIVLFIALIVFIMLLFANFAYLNTQANYDKQYIGHAGELRVLSQRIAKNATEAAAGKAAAFK
LLSDARNDFAQRWGYLKKGDPATGLPPAPSVVRPEMRAVQLDWERLLKNTDAILSSEQTVLSLHQVAATLAETVPQLQIE
YEKVVEILLQRGAPAAQVAMAQRQSLLAERILGAVNTVLSGDENSQQAADAFGRDAARFGQVLNGMLQGNPALKVSQVED
RDARARLSEISELFQFVSGSVDEILETSPELFKVRESATNIFSLSQTLLDEASHLASRFENLAGGRSTDTIGGYVLGLLA
LASIILIGMVMVRETNRQLRETAEKNERNQNAIMRLLDEIEDLADGDLTVTASVTEDFTGTIADSINYSVDQLRDLVATI
NLTAGQVAAAVQETQATAMHLAQASEHQAQQISEASTAISDMAQSIDQVSANAAESSAVAERSVEIANKGNEVVHNTIHG
MDNIREQIQDTAKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSAATRQ
IETLVRAIQTDTNEAVISMEQTTTEVVRGARLAQDAGVALEEIEGVSKTLAALIQSISNAAQQQTSSAGQISLTMNVIQQ
ITSQTSSGSTATAESIGNLAKMASQLRRSVSGFTLPTTPAPVADKA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=291, Percent_Identity=31.2714776632302, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1788195, Length=328, Percent_Identity=28.9634146341463, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1788194, Length=283, Percent_Identity=30.0353356890459, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787690, Length=366, Percent_Identity=27.5956284153005, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789453, Length=275, Percent_Identity=30.1818181818182, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 73412; Mature: 73412

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAKTGKPAEGSRSRSQIIVLFIALIVFIMLLFANFAYLNTQANYDKQYIGHAGELRVL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
SQRIAKNATEAAAGKAAAFKLLSDARNDFAQRWGYLKKGDPATGLPPAPSVVRPEMRAVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LDWERLLKNTDAILSSEQTVLSLHQVAATLAETVPQLQIEYEKVVEILLQRGAPAAQVAM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AQRQSLLAERILGAVNTVLSGDENSQQAADAFGRDAARFGQVLNGMLQGNPALKVSQVED
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEECCCCC
RDARARLSEISELFQFVSGSVDEILETSPELFKVRESATNIFSLSQTLLDEASHLASRFE
HHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLAGGRSTDTIGGYVLGLLALASIILIGMVMVRETNRQLRETAEKNERNQNAIMRLLDEI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EDLADGDLTVTASVTEDFTGTIADSINYSVDQLRDLVATINLTAGQVAAAVQETQATAMH
HHHCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAQASEHQAQQISEASTAISDMAQSIDQVSANAAESSAVAERSVEIANKGNEVVHNTIHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MDNIREQIQDTAKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCH
AVVADEVQRLAERSSAATRQIETLVRAIQTDTNEAVISMEQTTTEVVRGARLAQDAGVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHCCHH
EEIEGVSKTLAALIQSISNAAQQQTSSAGQISLTMNVIQQITSQTSSGSTATAESIGNLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KMASQLRRSVSGFTLPTTPAPVADKA
HHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKAKTGKPAEGSRSRSQIIVLFIALIVFIMLLFANFAYLNTQANYDKQYIGHAGELRVL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
SQRIAKNATEAAAGKAAAFKLLSDARNDFAQRWGYLKKGDPATGLPPAPSVVRPEMRAVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LDWERLLKNTDAILSSEQTVLSLHQVAATLAETVPQLQIEYEKVVEILLQRGAPAAQVAM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AQRQSLLAERILGAVNTVLSGDENSQQAADAFGRDAARFGQVLNGMLQGNPALKVSQVED
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEECCCCC
RDARARLSEISELFQFVSGSVDEILETSPELFKVRESATNIFSLSQTLLDEASHLASRFE
HHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLAGGRSTDTIGGYVLGLLALASIILIGMVMVRETNRQLRETAEKNERNQNAIMRLLDEI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EDLADGDLTVTASVTEDFTGTIADSINYSVDQLRDLVATINLTAGQVAAAVQETQATAMH
HHHCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAQASEHQAQQISEASTAISDMAQSIDQVSANAAESSAVAERSVEIANKGNEVVHNTIHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MDNIREQIQDTAKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCH
AVVADEVQRLAERSSAATRQIETLVRAIQTDTNEAVISMEQTTTEVVRGARLAQDAGVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHCCHH
EEIEGVSKTLAALIQSISNAAQQQTSSAGQISLTMNVIQQITSQTSSGSTATAESIGNLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KMASQLRRSVSGFTLPTTPAPVADKA
HHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]