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The map label for this gene is metF [H]

Identifier: 77461502

GI number: 77461502

Start: 5942362

End: 5943207

Strand: Direct

Name: metF [H]

Synonym: Pfl01_5281

Alternate gene names: 77461502

Gene position: 5942362-5943207 (Clockwise)

Preceding gene: 77461501

Following gene: 77461503

Centisome position: 92.3

GC content: 61.11

Gene sequence:

>846_bases
ATGTCCCAAGACCGTCGCTACAGCTTCGAGTTCTTCCCGACCAAGACCGATGCTGGGCATGAAAAACTGCTCGCCACTGC
CCGTCAGCTGGCCACCTATAAGCCTGACTTCTTTTCCTGCACCTACGGCGCTGGCGGTTCGACCCGTGACCGCACGCTGA
ACACCGTTCTGCAGCTGGAAAGCGAAGTCAAAATCCCCGCCGCACCGCACCTGTCGTGCGTCGGCGACAGCAAGGACGAC
CTGCGCGGCCTGCTGAACGAGTACAAGGCCGCCGGCATCAAGCGCATCGTCGCCCTGCGCGGTGACCTGCCGTCCGGCAT
GGGCATGACCAGCGGCGAGCTGCGTCACGCCAATGAACTGGTTGAATTCATTCGTGAAGAAACCGGCAATCATTTCCACA
TCGAAGTCGCCGCCTACCCGGAGATGCATCCGCAAGCGCGCAACTACGAAGACGATCTCGCCAACTTCGTGCGCAAGGCC
CGTGCCGGCGCCGACAGCGCGATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTCGACCGTTTGCAGGC
GCTGGGCGTGGACATTCCGGTGGTACCGGGGATCATGCCGATCACCAACTACAGCAAACTCGCGCGCTTCTCCGATGCCT
GCGGTGCGGAAATCCCGCGCTGGATCCGCAAGCAGCTGGAAGCCTACGGCGATGACAGCCAAAGCATTCAGCGCTTTGGC
GAACAAGTCGTCACGGAAATGTGCGAACGCCTGCTGCAAGGCGGCGCGCCCGGCCTGCACTTCTATTCCATGAACCAGGC
CGAACCAAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTAA

Upstream 100 bases:

>100_bases
CAAAACAGTGTCAGGCCGCAAGCTTCAGGCACAAGAGACCAGCGCACCTCCGCTGCTCTTGCAGCTTGAAGCTTGCAGCT
TGTAGCTGGAGGTCCCCTCC

Downstream 100 bases:

>100_bases
AAAGACGTAAACGCAGAACCCGAAGATCGCAGCCACGACGGCGCCTACAGCGCTTTCGCCGCTGCGATCTTTTGCTTTTA
ACGCCCGAAACAGAGCTTCT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSQDRRYSFEFFPTKTDAGHEKLLATARQLATYKPDFFSCTYGAGGSTRDRTLNTVLQLESEVKIPAAPHLSCVGDSKDD
LRGLLNEYKAAGIKRIVALRGDLPSGMGMTSGELRHANELVEFIREETGNHFHIEVAAYPEMHPQARNYEDDLANFVRKA
RAGADSAITQYFFNADSYFYFVDRLQALGVDIPVVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDSQSIQRFG
EQVVTEMCERLLQGGAPGLHFYSMNQAEPSLAIWNNLKLPR

Sequences:

>Translated_281_residues
MSQDRRYSFEFFPTKTDAGHEKLLATARQLATYKPDFFSCTYGAGGSTRDRTLNTVLQLESEVKIPAAPHLSCVGDSKDD
LRGLLNEYKAAGIKRIVALRGDLPSGMGMTSGELRHANELVEFIREETGNHFHIEVAAYPEMHPQARNYEDDLANFVRKA
RAGADSAITQYFFNADSYFYFVDRLQALGVDIPVVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDSQSIQRFG
EQVVTEMCERLLQGGAPGLHFYSMNQAEPSLAIWNNLKLPR
>Mature_280_residues
SQDRRYSFEFFPTKTDAGHEKLLATARQLATYKPDFFSCTYGAGGSTRDRTLNTVLQLESEVKIPAAPHLSCVGDSKDDL
RGLLNEYKAAGIKRIVALRGDLPSGMGMTSGELRHANELVEFIREETGNHFHIEVAAYPEMHPQARNYEDDLANFVRKAR
AGADSAITQYFFNADSYFYFVDRLQALGVDIPVVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDSQSIQRFGE
QVVTEMCERLLQGGAPGLHFYSMNQAEPSLAIWNNLKLPR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=272, Percent_Identity=34.9264705882353, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1790377, Length=270, Percent_Identity=39.2592592592593, Blast_Score=220, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI25147441, Length=266, Percent_Identity=35.3383458646617, Blast_Score=166, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25147436, Length=266, Percent_Identity=34.9624060150376, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6321313, Length=280, Percent_Identity=31.0714285714286, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6325234, Length=275, Percent_Identity=33.0909090909091, Blast_Score=144, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24662658, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31515; Mature: 31384

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDRRYSFEFFPTKTDAGHEKLLATARQLATYKPDFFSCTYGAGGSTRDRTLNTVLQLE
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
SEVKIPAAPHLSCVGDSKDDLRGLLNEYKAAGIKRIVALRGDLPSGMGMTSGELRHANEL
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VEFIREETGNHFHIEVAAYPEMHPQARNYEDDLANFVRKARAGADSAITQYFFNADSYFY
HHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
FVDRLQALGVDIPVVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDSQSIQRFG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHH
EQVVTEMCERLLQGGAPGLHFYSMNQAEPSLAIWNNLKLPR
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHCCCCCCC
>Mature Secondary Structure 
SQDRRYSFEFFPTKTDAGHEKLLATARQLATYKPDFFSCTYGAGGSTRDRTLNTVLQLE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
SEVKIPAAPHLSCVGDSKDDLRGLLNEYKAAGIKRIVALRGDLPSGMGMTSGELRHANEL
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VEFIREETGNHFHIEVAAYPEMHPQARNYEDDLANFVRKARAGADSAITQYFFNADSYFY
HHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
FVDRLQALGVDIPVVPGIMPITNYSKLARFSDACGAEIPRWIRKQLEAYGDDSQSIQRFG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHH
EQVVTEMCERLLQGGAPGLHFYSMNQAEPSLAIWNNLKLPR
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]