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The map label for this gene is betI

Identifier: 77461463

GI number: 77461463

Start: 5899685

End: 5900278

Strand: Direct

Name: betI

Synonym: Pfl01_5242

Alternate gene names: 77461463

Gene position: 5899685-5900278 (Clockwise)

Preceding gene: 77461461

Following gene: 77461464

Centisome position: 91.63

GC content: 60.44

Gene sequence:

>594_bases
ATGCCCAAGGTCGGTATGCAACCCATCCGCCGCCAACAACTGATCGAAGCCACTTTGCAGGCGGTTGATCAGGTCGGAAT
GGGGGACGCCAGCATTGCGCTGATCGCCCGTTTGGCCGGTGTCTCGAATGGCATCATCAGTCACTATTTTCAGGACAAGA
ACGGCCTGATTGCCGCCACGATGCGGTATCTGATGAGCGTCCTCAGCGAGAACGTCACCGCGCGCCGTCAGGCGCTGGCA
GACAGCAGCCCCCGGGCGCATCTGCAGGTGATCATCGAAGGCAACTTCGACGCCAGCCAGGTCAATGGCCCGGCAATGAA
AACCTGGCTGGCCTTCTGGGCCACCAGCATGCACCAGCCGTCTTTGCACAGGTTGCAGCGGATCAACGATCACCGTCTGT
ATTCCAACCTGTGCTGCGAGTTCCGCCGTGTGTTGCCGCTCGAAGATGCGCGCACCGCCGCCCGTGGACTGGCAGCTCTG
ATCGACGGCTTGTGGTTACGCGGCGCGCTGTCGGGAGACGCTTTCGACACTGCGCAGGCGCAACAGATCGCTTACGAATA
CATGGATTTCCAATTGGCCAAGCAGGTGAGCTAG

Upstream 100 bases:

>100_bases
TTATTCTTTGTTGATTGAACGTTCAATCAAAACAAAATAGACTGGCCCTCAATCCGATCGGCGTTTGTCGTCCGTCGGAA
GGCCTAAGGAGATGTGCAAG

Downstream 100 bases:

>100_bases
AGCACAGAAAAAACGCTCGGCCCCTGAACTGTCACCGACCAGCGATGCCACAGCTACATGGCTCACCCGGTGACCAACGC
CAACCACTAATGCACTTGCG

Product: transcriptional regulator BetI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MPKVGMQPIRRQQLIEATLQAVDQVGMGDASIALIARLAGVSNGIISHYFQDKNGLIAATMRYLMSVLSENVTARRQALA
DSSPRAHLQVIIEGNFDASQVNGPAMKTWLAFWATSMHQPSLHRLQRINDHRLYSNLCCEFRRVLPLEDARTAARGLAAL
IDGLWLRGALSGDAFDTAQAQQIAYEYMDFQLAKQVS

Sequences:

>Translated_197_residues
MPKVGMQPIRRQQLIEATLQAVDQVGMGDASIALIARLAGVSNGIISHYFQDKNGLIAATMRYLMSVLSENVTARRQALA
DSSPRAHLQVIIEGNFDASQVNGPAMKTWLAFWATSMHQPSLHRLQRINDHRLYSNLCCEFRRVLPLEDARTAARGLAAL
IDGLWLRGALSGDAFDTAQAQQIAYEYMDFQLAKQVS
>Mature_196_residues
PKVGMQPIRRQQLIEATLQAVDQVGMGDASIALIARLAGVSNGIISHYFQDKNGLIAATMRYLMSVLSENVTARRQALAD
SSPRAHLQVIIEGNFDASQVNGPAMKTWLAFWATSMHQPSLHRLQRINDHRLYSNLCCEFRRVLPLEDARTAARGLAALI
DGLWLRGALSGDAFDTAQAQQIAYEYMDFQLAKQVS

Specific function: Repressor involved in choline regulation of the bet genes

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786505, Length=188, Percent_Identity=59.5744680851064, Blast_Score=226, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BETI_PSEPF (Q3K5H5)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350970.1
- ProteinModelPortal:   Q3K5H5
- STRING:   Q3K5H5
- GeneID:   3716193
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5242
- eggNOG:   COG1309
- HOGENOM:   HBG684746
- OMA:   INHYFGG
- ProtClustDB:   PRK00767
- BioCyc:   PFLU205922:PFL_5242-MONOMER
- HAMAP:   MF_00768
- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR017757
- InterPro:   IPR001647
- Gene3D:   G3DSA:1.10.357.10
- TIGRFAMs:   TIGR03384

Pfam domain/function: PF00440 TetR_N; SSF46689 Homeodomain_like; SSF48498 TetR_like_C

EC number: NA

Molecular weight: Translated: 21827; Mature: 21696

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01081 HTH_TETR_1; PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVGMQPIRRQQLIEATLQAVDQVGMGDASIALIARLAGVSNGIISHYFQDKNGLIAAT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
MRYLMSVLSENVTARRQALADSSPRAHLQVIIEGNFDASQVNGPAMKTWLAFWATSMHQP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCH
SLHRLQRINDHRLYSNLCCEFRRVLPLEDARTAARGLAALIDGLWLRGALSGDAFDTAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QQIAYEYMDFQLAKQVS
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKVGMQPIRRQQLIEATLQAVDQVGMGDASIALIARLAGVSNGIISHYFQDKNGLIAAT
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
MRYLMSVLSENVTARRQALADSSPRAHLQVIIEGNFDASQVNGPAMKTWLAFWATSMHQP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCH
SLHRLQRINDHRLYSNLCCEFRRVLPLEDARTAARGLAALIDGLWLRGALSGDAFDTAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QQIAYEYMDFQLAKQVS
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA